k l and s in human breast cancer

... were expressed in insect cells, human cathepsin V was expressed in NSO cells, and human cathepsin L was isolated from liver Western blotting SDS-PAGE was performed, and protein was transferred ... Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer Binbin Chen and Manu O Platt* Abstract Background: Cathepsins K, L, and S are cysteine proteases upregulated in cancer and ... was binary: off in normal tissue and on in cancerous tissue Cathepsin K in breast tissue had the lowest variability and consistently low baseline for cathepsin K activity in the normal tissue,...

Ngày tải lên: 18/06/2014, 22:20

... Sampson J, Martin TA, Lee-Jones L, Watkins G, DouglasJones A, Mokbel K, Mansel RE: Tuberin and hamartin are aberrantly expressed and linked to clinical outcome in human breast cancer: The role ... most series reported on membrane staining and did not include cytoplasmic staining as a separate category Thus, it is possible that lobular carcinoma cases with cytoplasmic staining were included ... polymerase chain reaction does present some drawbacks The technique is highly sensitive and any contaminating cells (lymphocytes, stromal tissue) will lead to readings that belong to non cancer cells...

Ngày tải lên: 09/08/2014, 07:21

... damage, including single strand breaks (SSB) and alkali labile sites (ALS), was measured using the alkaline SCGE assay in DLD-1 cells treated with DCQ (5 μM, hour) IR (2 Gy) or combinations under ... relatively non-cytotoxic single-strand breaks DNA single strand breaks or alkali labile sites are by far the largest number of lesions in DNA in general Therefore, the decrease in cell survival and induction ... England) The bands were quantified using LabWorks 4.0 software Results Cell cycle modulation in four human colon cancer cell lines under oxia To study cell cycle modulation by DCQ+IR, cells were...

Ngày tải lên: 09/08/2014, 10:21

... expression status of breast cancer cell lines was confirmed using immunoblotting (Fig 3B) For breast cancer clinical samples, ERa-positive breast tumor specimens had significantly lower miR-22 levels ... experiments and differences were assessed using Student s t test The Kruskal–Wallis one-way analysis of variance test was used to test the significance of association between ERa status and the levels ... to assess the correlation between miR-22 expression and ERa protein levels in breast cancer cell lines and surgical specimens It was interesting to find that downregulation of miR-22 expression...

Ngày tải lên: 22/03/2014, 21:20

... water SA-b-galactosidase assay Expression of senescence-associated-b-galactosidase (SAb-gal) is increased in senescent cells [36] To determine if a-amylase treatment causes a change in cell senescence, ... Exclusively vimentinpositive cells were considered as fibroblasts, cytokeratinpositive or vimentin- and cytokeratin-positive cells were counted as epithelial cells Detection of cellular a-amylase ... different sensitivities of epithelial cells and fibroblasts towards trypsin For cell counting and passaging, trypsin/EDTA (0.15%) was used to detach cells, and its reaction was stopped with fetal calf...

Ngày tải lên: 10/08/2014, 10:21

... References Hadsell DL, Bonnette SG: IGF and insulin action in the mammary gland: lessons from transgenic and knockout models J Mammary Gland Biol Neoplasia 2000, 5:19-30 Rinaldi S, Toniolo P, Muti ... Rosner M, Fink-Retter A, Gschwantler-Kaulich D, Czerwenka K, Kroiss R, Tea M, Pischinger K, K stler WJ, Attems J, Mueller R, Blaukopf C, Kubista E, Hengstschläger M, Singer CF: Intratumoral IGF-I ... Holth A, Hellesylt E, Flørenes VA, Davidson B: Diagnostic and prognostic role of the insulin growth factor pathway members insulin-like growth factor-II and insulin-like growth factor binding...

Ngày tải lên: 11/08/2014, 00:23

... Horseradish peroxidase IL-2 Interleukin IL-10 Interleukin 10 JNK c-Jun N-terminal kinases MAPK Mitogen-activated protein kinase MAPKKK Mitogen-activated protein kinase kinase kinase xiv MKK3 ... of intracellular responses MAPKs (Mitogen-activated protein kinases) are protein Ser/Thr kinases that can initiate signaling cascades in response to extracellular stimuli, and this is often mediated ... milk from the breast to nipple Lobular carcinoma is originating from lobules, the parts of the breast that produce milk Classification of breast cancer is based on several aspects such as histopathology,...

Ngày tải lên: 10/09/2015, 09:09

... that luminal cluster was generally uniform across all samples, but basal-like cluster contained at least two major subsets including Basal A and B Basal A cluster matched closely to the Perou s ... the lobules are tiny bulb-like glands, or sacs, where milk is produced The lobes, lobules and glands are connected by thin ducts These ducts channel milk to openings in the nipple The lobules are ... breast- cancer stem cells Basal-like breast cancers often display geneexpression patterns that are consistent with those of cells undergoing epithelial to mesenchymal transition Basal-like breast...

Ngày tải lên: 10/09/2015, 15:51

... health knowledge and skills In all the FGDs, women talked about seeing or hearing about breast cancer and breast health examinations on TV, radio, billboards, doctor s clinics and newspapers In addition, ... told that some husband s had misconceptions about breast cancer being a transmissible illness Men s knowledge about breast cancer and their attitudes towards their partner s breast cancer screening ... femininity” (3, 8) In our society a woman is manipulated as a toy, a man whose wife gets inflicted with breast cancer, this hits his masculinity and usually immediately his eyes starts wandering...

Ngày tải lên: 13/02/2014, 06:20

... similar in all three cell lines The differences in response to DENSPM treatment are presumably reflected in other genetic lesions in the cell lines One difference between MALME-3M and SK-MEL-28 cells ... of spermidine/ spermine N1-acetyltransferase (SSAT) in L5 6Br-C1 cells Cells were seeded in the absence or presence of 10 lM DENSPM The results are presented as mean values (n ¼ 6) and bars represent ... DENSPM treatment In L5 6Br-C1 cells, the DENSPM-induced increase in SSAT activity was not as extensive as in any of those two cell lines The depletion of the polyamine pools was however, similar...

Ngày tải lên: 17/03/2014, 23:20

... concentration used The absence of major toxicity to normal cell lines (human intestinal cells in this study and mouse intestinal cells and mouse mammary cells in previous studies) makes DCQ an interesting ... protein increased in response to DCQ in all three cell lines except in p53+/+ cells exposed to hypoxia (Figure 6A); p21 protein also increased in all cell lines except in p53+/+ cells exposed ... and in EMT6 mouse mammary carcinoma cells and Lewis Lung Carcinoma (LLC) cells [4,8] We also showed that DCQ inhibited cell proliferation and induced apoptosis in colon T-84 cancer cell lines...

Ngày tải lên: 09/08/2014, 09:20

... manufacturer s instructions (Pierce) ELISA assay For ELISA assays, × 104 U-937 and THP-1, as well as CALO and INBL, cells were plated in 48-well plates for days The cell culture supernatants were collected ... proteins in two human epithelial cervical cancer cell lines, CALO and INBL, using polyclonal antibodies against MICA/MICB and anti-NKG2D for western blot and flow cytometric analyses Our results show ... NKG2D staining (C) of CALO (left panels) and INBL (right panels) cells Note the strong cytoplasmic staining in both cell lines (Original magnification × 40) Figure Cervical cancer cell lines secrete...

Ngày tải lên: 10/08/2014, 10:21

... have related aminoacid sequences and are well conserved between species Class I-btubulin is the most commonly expressed isotype in human beings, and the most common isotype in cancer cells [42] ... high and low expressing tumors, staining was considered high in 11 (30.5%) out of 36 tumors in our series (similar results were obtained using as cut-off the median value) P53 expression levels ... response n .s n .s n .s = not significant; CR = complete response; PR = partial response; SD = stable disease; PD = disease progression #According to Elston and Ellis classification (Ref 31) *According...

Ngày tải lên: 10/08/2014, 10:21

... family genes are cancer- associated genes [3] JMJD2A is widely expressed in human tissues and cell lines, and high endogenous expression of JMJD2A mRNA was found in several cell types, including human ... Figure Knock down of JMJD2A resulted in suppressing tumor cell invasion A Cells in blank control group transversed the Transwell membrane B Cells in negative control group C Cells in siRNA group ... deficits in mice Neuroscience 2011, 184:54-63 11 Dai HY, Liu L, Qin SK, He XM, Li SY: Lobaplatin suppresses proliferation and induces apoptosis in the human colorectal carcinoma cell Line LOVO in vitro...

Ngày tải lên: 10/08/2014, 10:21

... reported in various tumors, including gliomas, breast tumors and stomach adenocarcinoma Hepatocellular carcinoma (HCC) patients with low ING4 expression had poorer overall survival and disease-free survival ... The detailed mechanisms and signaling pathways regulated by miR-15a and miR214 in pancreatic cancer deserve further study Materials and methods Cell cultures and clinical samples BxPC-3 human pancreatic ... viability study, cytotoxicity was determined in BxCP-3 cells treated with GEM using the CCK-8 assay Cells were plated at × 104 per well in a 96-well plate and allowed to adhere for hrs The cells...

Ngày tải lên: 10/08/2014, 22:21

... Barbatis C, Lea RW, Kalofoutis A, Korkolopoulou P: Analysis of interleukin (IL)-8 expression in human astrocytomas: associations with IL-6, cyclooxygenase-2, vascular endothelial growth factor, and ... days turning them at regular intervals to achieve nearly complete vasculogenesis and blood vessel development progressing mostly through angiogenesis On day 10, the eggs were candled and a small ... immunity plays a crucial role in angiogenesis, organogenesis, and the pathogenesis of various diseases including tumor development, growth and metastasis, atherosclerosis, sepsis, and rheumatoid...

Ngày tải lên: 11/08/2014, 00:22

... Journal of Medical Case Reports 2008, 2:126 Clinically, he had a lump centrally in the left breast, at a o'clock position, with skin tethering and mild inversion of the nipple Ultrasound (US) and ... III axillary clearance was performed Overall, one out of nine dissected lymph nodes was positive Our well-established protocols for wide local excisions rely on mm pathological clear margins rather ... axillary node clearance; BCS: breast conserving surgery; DCIS: ductal carcinoma in situ; FBC: female breast cancer; FNA: fine needle aspiration; IDC: invasive ductal carcinoma; MBC: male breast...

Ngày tải lên: 11/08/2014, 23:21

... liver cancer (Kuo et al., 2001) 17 Scutellaria barbata (SB) (Skullcap) contains flavonoids (scutallarein, scutellarin), sesquiterpenes, wogonin, lignin, resin etc It is known to have anti-inflammatory, ... and inflammatory responses It is produced by different activated cells including macrophages, granulocytes, T and B lymphocytes, natural killer (NK) cells, fibroblasts, and certain tumor cells ... disease fighting pattern are used simultaneously, assuming that groups of chemicals in these herbs produce a cluster of actions on various cells, cellular organs and multiple receptors, thus...

Ngày tải lên: 15/09/2015, 17:10

... prognostic indicator, seminal vesicle invasion, is defined as cancer invading into the muscular coat of the seminal vesicle (Ohori et al., 1993) In most cases, it occurs in glands with EPE (Epstein ... minute SDS-PAGE sodium-docedyl-sulphate polyacrylamide gel electrophoresis SHH Sonic Hedgehog shRNA short hairpin RNA siRNA silencing RNA SVI seminal vesicle involvement/invasion TGF transforming ... 2010) has indicated a downregulation of HS3ST3B1 in both prostate cancer cell lines and tissues The expression level of HS3ST3B1, a gene involved in heparan sulphate biosynthesis, was verified in...

Ngày tải lên: 05/10/2015, 22:31