küpffer cells and endothelial cells
báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
Ngày tải lên :
20/06/2014, 01:20
... these cells seeded on it Figure tion Effects of composite scaffolds on the cells viability and funcEffects of composite scaffolds on the cells viability and function The induced osteoblasts and endothelial ... for cell process and spreading, adhesion and orientation Therefore, osteoblasts and endothelial cells were inclined to attach to the rougher surface and appeared better viability and strong cellular ... (arrows), and cellular projection (1500×) Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and...
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![Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc](https://media.store123doc.com/images/document/2014_08/12/medium_9S3XH52qK4.jpg)
Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc
Ngày tải lên :
11/08/2014, 08:21
... Materials and Methods section (n = 3) Effects of NF161 and NF177 on PGE2 and PGI2 production by pre-induced COX-2 IL-1α (10 ng/ml) was added and incubated for 20 h The cells were washed and NF161 ... contributions CD and MC participated in the study design, carried out the experiments and wrote the manuscript MG performed the ELISA experiments GR and MD designed and synthesized NF161 and NF177 RF ... was 51 ± cells/ microscope fields (n = 30) The direct effect on HUVEC was assessed by pretreated the cells with the 1, 8-naphtyridines (10-4M) and 10-7M PAF for 20 min, washed three times and challenged...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
Ngày tải lên :
14/02/2014, 18:20
... for 60 s, 22 cycles and 23 cycles for CYP1A2 and CYP2B1, respectively; and 94 °C for 30 s, 54 °C for 30 s, 72 °C for 60 s, 26 cycles, 25 cycles and 25 cycles for TNF-a, IL-6 and b-actin, respectively ... Zhang Y, Yang H & Yang LX (2007) [Expression of HMGB-1 and its extracellular release of cultured primary hepatic parenchymal cells and Kupffer cells induced by LPS] Zhonghua Gan Zang Bing Za Zhi ... liver and spleen macrophages in gadolinium chloride-induced elimination and repopulation J Leukoc Biol 52, 296–302 Xu DX, Wei W, Sun MF, Wu CY, Wang JP, Wei LZ & Zhou CF (2004) Kupffer cells and...
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Báo cáo y học: " Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress" doc
Ngày tải lên :
13/08/2014, 13:20
... modifications of rat liver cells induced by lead nitrate Ital J Zool 1998, 65:141-148 Arii S and Imamura M: Physiological role of sinusoidal endothelial cells and Kupffer cells and their implication ... released by endothelial cells or by other nonparenchymal liver cells It is worth noting that endothelial liver cells show the highest resistance to in vivo Pb(NO3)2 injection, whereas Kupffer cells ... and performed the flow cytometry LD conceived the study and participated in its design and coordination All the authors read and approved the final manuscript Hepatocytes, HepG2 and Kupffer cells...
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Báo cáo y học: "Characterization of Kupffer cells in livers of developing mice" ppt
Ngày tải lên :
13/08/2014, 13:20
... positive cells and standard deviation for each animal was calculated, and the overall mean number of cells with standard errors was calculated for each cell type and age The numbers of labelled cells ... Fluorescence images comparing F4/80 positive cells and albumin positive cells A: Merged image showing green F4/80 positive cells and red microsphere positive cells B: Same region as in ‘A’ photographed ... hepatocytes and nonhepatocytes in the rat liver parenchyma A stereological study J Cell Biol 1977, 72:441-455 Fahimi HD: Sinusoidal endothelial cells and perisinusoidal fat-storing cells: structure and...
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Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx
Ngày tải lên :
18/02/2014, 06:20
... contribute to endothelial dysfunction and microvascular disease and reduced androgen levels; (b) the insult on the endothelium resulting in endothelial injury and reduced NO synthase activity and NO ... including obesity-related androgen deficiency and obesity-induced endothelial dysfunction Obesity promotes inflammatory responses and contributes to endothelial dysfunction The role of endothelial function ... components and improves endothelial function [108– 111] Testosterone therapy in men with androgen deficiency demonstrated an improvement in erectile function [112–114] and increased muscle mass and decreased...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
Ngày tải lên :
21/02/2014, 01:21
... synthesis in cells cultured under standard conditions This effect was evident after 24 h of treatment and was still consistent in cells exposed to the ruthenium compound for 48 and 72 h, indicating ... gene expression and MEK/ERK phosphorylation (Eur J Biochem 269) 5869 ERK1/2 activation and activity was attained in a transformed endothelial cell line and it is now evident that the endothelial ... associates with tumor growth and metastasis [12], and in this context, NAMI-A has been shown to inhibit angiogenesis [41] and to induce apoptosis in endothelial cells [42] In conclusion, the...
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Báo cáo khoa học: Intermittent hypoxia is a key regulator of cancer cell and endothelial cell interplay in tumours pot
Ngày tải lên :
30/03/2014, 04:20
... hypoxia on cancer cells and endothelial cells within a tumour Fig Schematic representation of the effects of HIF-1 activation under intermittent hypoxia on cancer cells and endothelial cells within ... i.e the endothelial cells, must also be considered Indeed, the tumour environment induces faster endothelial cell proliferation than in normal tissue, and the turnover of endothelial cells in ... approximately 50–100 tumour cells [93] Therefore, the destruction of a few endothelial cells may induce the death of a large number of tumour cells Moreover, it was shown that endothelial cell suppression...
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Báo cáo y học: "Carotid intima-media thickness and endothelial function: useful surrogate markers for establishing cardiovascular risk in patients with inflammatory rheumatic disease" pdf
Ngày tải lên :
09/08/2014, 10:23
... of print] Gonzalez-Juanatey C, Llorca J, Garcia-Porrua C, SanchezAndrade A, Martín J, Gonzalez-Gay MA: Steroid therapy improves endothelial function in patients with biopsy-proven giant cell arteritis ... References Veldhuijzen van Zanten JJCS, Kitas GD: Inflammation, carotid intima-media thickness and atherosclerosis in rheumatoid arthritis Arthritis Res Ther 2008, 10:102 Gonzalez-Juanatey C,...
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Báo cáo y học: "Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers." pptx
Ngày tải lên :
11/08/2014, 08:22
... those of leukocytes and endothelial cells Therefore the study was undertaken to test the hypothesis that lower limb orthopaedic surgery results in changes to leukocyte and endothelial markers ... between 58 and 87 years old (mean age = 77 for both THR and TKR), and were all scheduled for elective surgery due to osteoarthritis patients were scheduled for THR (3 females and males) and patients ... vascular disease and during surgical procedures, which involve the application of a tourniquet, such as upper limb (e.g fasiectomy and carpal tunnel) and lower limb (e.g knee arthroplasty and TKR) orthopaedic...
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Báo cáo y học: "Involvement of Akt and endothelial nitric oxide synthase in ventilation-induced neutrophil infiltration: a prospective, controlled animal experiment" docx
Ngày tải lên :
13/08/2014, 08:20
... PI3-K and Akt pathway in an isolated mouse model of endotoxemia [14,15] Dimmeler and colleagues exposed human umbilical vein endothelial cells to shear stress in a cone-plate viscometer [19], and ... (Akt) expression ((a) and (b), top panel) and an antibody that recognizes total Akt protein expressions in lung tissue ((a) and (b), middle panel) from control nonventilated mice and from mice ventilated ... on endothelial nitric oxide synthase activation Phosphorylated endothelial nitric activation oxide synthase (eNOS) expressions ((a) and (b), top panel), total eNOS protein expressions ((a) and...
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Báo cáo y học: "Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury" potx
Ngày tải lên :
13/08/2014, 21:21
... numbers 315/99 and 69/2003A) and informed consent, human umbilical venous endothelial cells (HUVEC) and polymorphonuclear neutrophils (PMN) were derived from human umbilical veins and citrated ... Dickinson, San Jose, CA, USA) Cells were gated using forward and side scatter properties and staining with Distinct from true endothelial activation, severe sepsis leads to endothelial cell injury ... Authors’ contributions AP and BN conceived of the study, participated in its design and coordination, and drafted the manuscript VS and AH carried out the adhesion assays JR and HAH participated in...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... cells were suspended with 500 lL MACS buffer and applied onto MACS columns Magnetically selected positive cells and depleted cells were cultured independently as lymphatic endothelial cells and ... murine endothelial cells from various developmental stages and locations is important for dissecting molecular and cellular mechanisms of endothelial development and function Murine primary cells, ... dishes and cultured at 33 °C (day in Fig 1) For the initial weeks, primary cells, including both tsA58T Ag-negative cells (primarily fibroblasts) and tsA58T Ag-positive cells, proliferated, and ECs...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... constitutively expressed fgl2 at low levels in cells or tissues including cytotoxic T cells, trophoblasts, intestinal epithelial cells and endothelial cells remains to be defined We hypothesize that ... structural and functional characterization of the 5¢-flanking region of the mouse fgl2 gene in vascular endothelial cells Through a series of studies focusing on constitutive expression in endothelial cells, ... loading and first strand synthesis with forward primer, 5¢-ATGTTTGAGACCTTCAACAC-3¢, and reverse primer, 5¢-CACGTCACACTTCATGAT GGA-3¢ then excised from PCR2.1 by digestion with HindIII and SalI and...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
Ngày tải lên :
16/03/2014, 18:20
... vein endothelial cells (HUVECs) and rabbit coronary endothelial cells have reported a redistribution of cPLA2-a to the nuclear envelope [26,27] The localization of cPLA2-a in human endothelial cells ... above and with those seen 1281 cPLA2-a localization in endothelial cells A S Grewal et al B C Fig The location and relocation of cPLA2-a in Triton X-100- and digitonin-permeabilized cells (A) Cells ... cPLA2-a expression levels and subcellular distribution in EA.hy.926, HUVEC, A549 and HeLa cells (A) Total cell lysates from EA.hy.926, HUVEC, A549 and HeLa cells were prepared and 20 lg protein of...
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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt
Ngày tải lên :
23/03/2014, 09:20
... vein endothelial cells [15], vascular smooth muscle cells [13], lens epithelial cells [16], and some tumor cell lines such as B16 melanoma [17] and P19 embryonal carcinoma cells [18] Both GSK3b and ... biogenesis and mass within cells and tissues reflect changes in energy demand and hormonal status, and depend mainly on changes in the activity and ⁄ or expression of the transcription factors NRF1 and ... cerebellar cells [19] and enrichment of active GSK3b in mitochondria and nuclei has also been observed in neuronal cells [20] In the immortalized neuronal cell line SH-SY5Y, mitochondrial and active...
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Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf
Ngày tải lên :
30/03/2014, 01:20
... including MAP kinases and NF-jB [12,23,24] Previous studies have demonstrated that LPS and AGEs activate MAP kinases in various cells, including monocytes ⁄ macrophages and endothelial cells [12,24,25] ... effect of AGEs and LPS on endothelial cells, and, furthermore, the underlying signal mechanisms involved, have not been fully understood In the present study, we found that both AGE-HSA and LPS, in ... amplify the inflammatory response in endothelial cells In the present study, we show that stimulation of human endothelial cells with a combination of AGEs and LPS demonstrates a synergistic effect...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
Ngày tải lên :
30/03/2014, 13:20
... treatment Ouabain was added to the cells at various concentrations in fresh ECGM0.5, and the cells incubated for an additional 12 h For counting, cells were detached and dispersed in trypsin/ EDTA ... smooth muscle cells of the artery As this substance is a potent vasoconstrictor and growth promoter of vascular smooth muscle cells via the ETA receptor, and because vesicular fusion and release ... independently of ouabain Ouabain was added to the cells at various concentrations in ECGM-SF, and the cells were further incubated at 37 °C for 30 The cells were then lysed using a commercially available...
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báo cáo hóa học:"Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells" pdf
Ngày tải lên :
18/06/2014, 15:20
... CD66ae and CD66de mAbs, but they remained responsive to CD66b, CD66c, and CD63 mAbs (Fig 1, panel D) When cells were desensitized to CD66b and CD66c mAbs, the cells were unresponsive to CD66b and ... washed, and Ca2+ free buffer containing IgG (panel A), the CD66ae mAb and CD66b mAb (panel B), the CD66ae mAb and CD66c mAb (panel C), the CD66ae mAb and CD66de mAb (panel D), the CD66b mAb and CD66c ... to endothelial cells Because CEACAM-1, -3, -6, and -8 are highly homologous structurally, and can undergo a number of different http://www.translational-medicine.com/content/6/1/78 homotypic and...
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