journal of oral and maxillofacial surgery medicine and pathology elsevier
Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
... order of magnitude below the s H of AfM1PDH. Discussion Kinetic mechanism of Af M1PDH and Af M2DH The theory developed by Cook and Cleland is used to deduce the kinetic mechanism of AfM1PDH and AfM2DH ... binding of Man-ol1P and NAD + by AfM1PDH is not ordered, and that there is also randomness in the binding of substrate and coen- zyme in each direction of the reaction catalyzed by AfM2DH. By way of ... also reported random binding of NAD + and Man-ol1P [29]. It is reasonable to assume that random binding of reactants by AfM1PDH and AfM2DH occurs in rapid equilibrium, and the absence of curvature...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Methylene analogues of adenosine 5¢-tetraphosphate Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases pot
... from human and the narrow-leafed lupin. ppCH 2 ppA and pCH 2 pppA, inhibited the human enzyme with K i val- ues of 1.6 and 2.3 nm, respectively, and the lupin enzyme with K i values of 30 and 34 ... calculated according to the method of Dixon and Webb [46] from the slopes of plots v ⁄ v i against [I] (where v and v i are velocities in the absence and presence of inhibitor, respectively, and [I] is the inhibitor ... Mark J. Thompson 3 and G. Michael Blackburn 3 1 Department of Biochemistry and Biotechnology, Agricultural University, Poznan ´ , Poland 2 Department of Biophysics, Institute of Experimental Physics,...
Ngày tải lên: 16/03/2014, 14:20