iviat to identify virulence factors of porphyromonas gingivalis
Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Ngày tải lên :
07/08/2014, 18:21
... showed coefficient of similarities from 63% to 80% level The discriminatory power (D-value) of this RAPD fingerprinting assay was 0.86 Vero cell cytotoxic assay Cytotoxic effects of E coli O157:H7 ... multiplex PCR for identifying the presence of several virulence factors Conventional Culture Method After secondary selective enrichment procedures with 90 ml of mEC broth, one loopful of the broth ... taken in the conical tube (Becton Dickinson, USA), and placed into the ice for transport to the laboratory within h Ten ml of each sample was transferred into 90 ml of BPW for preliminary enrichment...
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Báo cáo khoa học: " Respiratory viral infections detected by multiplex PCR among pediatric patients with lower respiratory tract infections seen at an urban hospital in Delhi from 2005 to 2007" docx
Ngày tải lên :
12/08/2014, 04:22
... Ward of All India Institute of Medical Sciences (AIIMS) Hospital, New Delhi, India The demographic profile of child, clinical symptoms and risk factors were recorded in a predesigned proforma ... showing band of 683 bp, Influenza A of 105 bp, Influenza B of 503 bp Lane 3: Marker 100 bp ladder Lane 4: Amplicon for PIV1 showing band of 84 bp, PIV2 of 197 bp, PIV3 of 266 bp, and hMPV of 440 bp ... respiratory viruses Of these 106 children with ALRI, 64 presented to the OPD and 42 were admitted to the ward Of the 64 children who presented to OPD, 52 had ALRI and 12 had severe ALRI Of the...
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Báo cáo khoa học: "Impact of coccidial infection on vaccine- and vvIBDV in lymphoid tissues of SPF chickens as detected by RT-PCR" doc
Ngày tải lên :
12/08/2014, 18:21
... one and 25% of the follicles was assigned a value of 1, 26 to 50% was assigned a value of 2, 51 to 75% was assigned a value of 3, and 76 to 100% affected follicles was assigned a value of Serology ... involved 1–25% of the follicles, a value of was assigned, 26–50% of follicles a value of was assigned, 51–75% of follicles a value of was assigned, and 76–100% of follicles a value of was assigned ... The objective of our study was investigation of the effect of the presence of coccidia on the tissue distribution of vaccine and vvIBDV In a previous paper, we documented indications of vvIBDV replication...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
Ngày tải lên :
05/09/2013, 10:15
... Bureau, the concentrations of phytoplankton were increased from autumn to spring of 2005 to 2006 The abundance of microcystin – degrading bacteria was positively related to the chlorophyll concentration ... reactions required µL of extracted DNA, 10 pmol of each primer, 30 pmol of TaqMan probe, 25 µL of TaqMan Gene Expression Master Mix (Applied Biosystems Co USA), made up to a final volume of 50 µL using ... and the abundance ratio of the microcystin-degrading bacteria increased to approximately 0.005% of the total bacteria The number of cells increased in fall, and the number of microcystin-degrading...
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Cloning genes by PCR
Ngày tải lên :
25/10/2013, 22:20
... In essence the procedure is identical to the generation of a library by ligation of a double-strand adaptor The adaptor is added to the 5′- and 3′-ends of each molecule in the library whether ... It is convenient to use PCR as a tool to rapidly screen random clones to determine the quality of a cDNA library Essentially random plaques are transferred with a toothpick to a PCR mix and universal ... included to amplify the full-length product B Isolation of unknown DNA sequences It is often of interest to isolate and clone unknown DNA fragments that lie adjacent to already cloned regions of DNA...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
Ngày tải lên :
18/02/2014, 13:20
... contrast to Ste20p, Cla4p does not bind to Erg4p, Cbr1p or Ncp1p A total of 105 cells of the indicated plasmid combinations were spotted either onto medium lacking histidine and leucine to select ... consisting of light petroleum ⁄ diethyl ether (49 : 1, v ⁄ v) To visualize separated bands, TLC plates were dipped into a charring solution consisting of 0.63 g of MnCl2Æ4H2O, 60 mL of water, 60 mL of ... protein concentration of 10 mL of culture with an attenuance at 600 nm of was determined and cells were incubated for h at 90 °C together with 0.6 mL of methanol, 0.4 mL of 0.5% pyrogallol dissolved...
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Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot
Ngày tải lên :
07/03/2014, 04:20
... activity of the glutaredoxin, rendering it less toxic to the cell Remarkably, the phospho-mimic substitution S134D was able to restore the toxicity of the glutaredoxin, supporting the relevance of ... forms of the BUD32 sequence, both fused to the His epitope Cells were grown until exponential phase in galactose medium to induce the ectopic expression of the plasmid-borne genes, and aliquots of ... physiological target of Sch9p, representing one of the effectors of this protein kinase known to be involved in multiple cellular processes A B Fig Phosphorylation at Ser258 of Bud32p is related to Sch9p...
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Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc
Ngày tải lên :
07/03/2014, 16:20
... Laboratorie Europe, Exeter, UK) or Ac-CCK-33-Gly (Cambridge Research Biochemicals, Stockton, UK) as substrate, 20 lL yeast extract, various inhibitors and activators in a total volume of 30 lL ... The nature of the protease performing the cleavage of synthetic human CCK-33 to CCK-22 was analysed by inclusion of a number of inhibitors with the extract from BJ2168 Only the addition of a metal ... MITOP program predicts that Cym1p localizes to the mitochondria via an N-terminal signal peptide [25] To investigate whether the intracellular localization of Cym1p is due to residues 1–30 of...
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Báo cáo Y học: Acceleration of granulocyte colony-stimulating factor-induced neutrophilic nuclear lobulation by overexpression of Lyn tyrosine kinase docx
Ngày tải lên :
17/03/2014, 17:20
... reported to be associated with the G-CSF receptor, but their physiological roles are not clearly understood In avian hematopoietic Lyn-de®cient cells, ectopic expression of the human G-CSF receptor ... Tryptophan 650 of human granulocyte colony-stimulating factor (G-CSF) receptor, implicated in the activation of JAK2, is also required for G-CSF-mediated activation of signaling complexes of the ... factor-induced dierentiation of myeloid cells J Biol Chem 272, 25184±25189 49 Rodel, J.E & Link, D.C (1996) Suppression of apoptosis during cytokine deprivation of 32D cells is not sucient to...
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Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc
Ngày tải lên :
22/03/2014, 17:20
... pathway As mTOR is one of several downstream targets of PI3K and because LY294002 is also an mTOR inhibitor [26], it is possible that mTOR is also involved in LPS-induced Oct-2 expression To test ... rapamycin for the treatment of inflammatory diseases As G-CSF can function as either an anti-inflammatory or a proinflammatory cytokine, understanding the mechanism of the effect of rapamycin on G-CSF ... transfected with 7.5 lg of empty vector or plasmid encoding DN-mTOR (D) or RR-mTOR (E), then, 24 h later, were incubated for h with or without LPS in the absence or presence of 200 ngÆmL)1 of rapamycin;...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
Ngày tải lên :
23/03/2014, 11:20
... used at 10 mm The blocking of the ATPase activating effect of glucose was not the result of a toxic effect of 2-deoxy-d-glucose, as subsequent incubation in the presence of 10 mm glucose resulted ... Dissociation of tubulin–H+-ATPase complex by glucose of ATP hydrolysis in the presence vs absence of 100 lm sodium orthovanadate Isolation and determination of HAT HAT was isolated into the Triton X-114 ... plasma membrane, and found them to be similar to those of acetylated tubulin in mammalian cells After alkaline treatment, yeast plasma membrane was depleted of total tubulin (determined with DM1A...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
Ngày tải lên :
18/06/2014, 15:20
... applied to the monitoring of immune responses in PBMC [7], in tumor specimens [8,9] or to the identification of antigenic epitopes [10-12] We sought to further develop these methods into a simple ... axes) Page of (page number not for citation purposes) Journal of Translational Medicine 2008, 6:58 However, the correlation of specific profiles of cytokine gene expression with markers of protection ... stimulation of cytokine gene expression could emerge as an important tool for the screening of cellular immune response to large panels of antigens or peptides and the rapid identification of novel...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
Ngày tải lên :
18/06/2014, 16:20
... annealing to each of 22 different Vb subgroups (36 Vb genes) to quantify expansion of T cell bearing specific Vb subgroups and subgroup genes in response to SAgs One of the important factors that ... This qRT-PCR method could also be used to characterize novel SAgs, to determine complete profiles of currently known SAgs, and to help understand the role of T cells bearing specific Vbs in certain ... the change in the fluorescence of the reporter dye (ΔRn) crossed the threshold To synchronize each experiment, the baseline was set automatically by the software To rule out DNA contamination...
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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
Ngày tải lên :
19/06/2014, 08:20
... http://www.virologyj.com/content/2/1/61 Table 2: Relationship of HSV CVL viral load with potential risk factors Cofactor Geometric mean number of copies of virus/ml of lavage fluid Present GUD Anti HSV-2 IgG HIV-1 infection BV Candida ... decreased the sensitivity of typing assay [24] The present study used assays based on Taqman-MGB probes, to identify the HSV type in a population of symptomatic and asymptomatic patients attending ... peak of the correct Tm in dissociation or melting curve analysis plots The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL The lower limit of quantitation of...
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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
Ngày tải lên :
20/06/2014, 04:20
... http://www.virologyj.com/content/2/1/61 Table 2: Relationship of HSV CVL viral load with potential risk factors Cofactor Geometric mean number of copies of virus/ml of lavage fluid Present GUD Anti HSV-2 IgG HIV-1 infection BV Candida ... decreased the sensitivity of typing assay [24] The present study used assays based on Taqman-MGB probes, to identify the HSV type in a population of symptomatic and asymptomatic patients attending ... peak of the correct Tm in dissociation or melting curve analysis plots The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL The lower limit of quantitation of...
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Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
Ngày tải lên :
07/08/2014, 14:22
... from µg to 100 pg and subjected to PCR reaction to determine the sensitivity of the PCR assay The detection limit of the PCR with 35 cycles was in the range up to ng of template DNA (Fig 2) To increase ... corresponded to nucleotides 110 to 132 and 297 to 319, respectively, and defined a 210bp DNA fragment on PCR reaction PCR mixture (50 µl) contained µl of 10 PCR buffer, µl of 25 mM MgCl2, µl of 10 ... swine genomic DNA The number of amplification cycles was one of the important factors for increasing the sensitivity of the PCR assay The increase in the number of amplification cycles may produce...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
Ngày tải lên :
07/08/2014, 18:21
... possible to detect fastidious and hard -to- culture bacteria like Ehrlichia spp without the need of isolation by conventional culture methods PCR makes it possible to identify the presence of DNA of ... region of 16S rRNA gene were used [1] The PCR mix for the first round PCR consisted of 2.5 µl of 10X PCR buffer, 2.5 µl of 25 mM MgCl , µl of 2.5 mM deoxynucleoside triphosphate (dNTPs), 2.5 U of ... according to the manufacturer’s instructions Purified amplicons were ligated into pGEM-T easy vector (Promega, USA) as per the instructions given by the manufacturer and transformed into TOP10F’...
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Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
Ngày tải lên :
07/08/2014, 18:21
... ces 003 rof C 27 ta noisnetxe lanif dna ces 09 rof C 27 pmet noisnetxe ,ces 54 rof C 06 pmet gnilaenna ,ces 54 rof C 49 ta noitarutaned ,ces 06 rof C 49 ta noitarutaned laitini ,sgniwollof eht ... dlof 8.1 naht ssel ot A3PYC decudni nicipmafir dna NCP ,enosahtemaxed ,684 -UR A3PYC gnicudni scitoibonex fo sessalc rehto eht naht rehgih hcum snoitcudni dlof 8.3 gniwohs A3PYC fo recudni tnetop ... stibihni dica ciadiakO ]9[ )Mµ 001( niloksrofyxoedid ,1 dna )Mµ 001( niloksrof ,)Mµ 004( BP htiw srh 61 rof sllec amotapeh nekcihc gnitaert retfa dnuof osla saw 73A3PYC fo slevel tpircsnart fo...
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