its role in the connection of wind power to power networks
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... for increased proteolysis, apoptosis and glial cell activation Copper homeostasis is important in the brain, therefore the role of copper binding or loss of its binding could be related to specific ... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... understanding what causes the loss of appropriate metal binding might be crucial for the understanding of the role of amyloidogenic proteins in a number of neurodegenerative disorders Copper binds to...
- 14
- 586
- 0
Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
Ngày tải lên :
08/03/2014, 16:20
... 20 lL of the enzyme/inhibitor mixture to 180 lL of substrate (4NPGlc), incubating for 30 at 37 °C, and measuring the release of 4NP Protein assays and protein sequencing Total protein in crude ... containing arbutin or salicin as substrate; 100% C initial, increasing D to 20% (10 min), 50% (15 min), 100% (5 min), held at 100% (5 min); cyanodin glycosides; 5% B/95% A initial (5 min), increasing ... from a single gene Expression of cbg-1 in P pastoris The cDNA sequence encoding the entire human liver cbg-1 cDNA was inserted into the expression vector pHIL-S1 in frame with the P pastoris phosphatase...
- 10
- 775
- 0
Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
Ngày tải lên :
23/03/2014, 17:21
... mislocalization of all three enzymes in these cells clearly shows the involvement of PEX14 in the targeting of PTS-1, PTS-2 and I-PTS proteins to the glycosome To further analyse the effect of the destruction ... N-terminal domain of the polypeptide of each of these organisms is followed by a stretch of hydrophobic amino acids that is presumably involved in the protein’s anchoring to the membrane Also in ... proteins from the cell at lower concentrations of digitonin than in untreated cells (Fig 4) We interpret this observation as the retainment of newly synthesized proteins in the cytosol due to the...
- 9
- 549
- 0
Báo cáo y học: " Th2 cytokines and asthma Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists" pot
Ngày tải lên :
12/08/2014, 18:20
... involves its interaction with receptors consisting of a heterodimer of high-affinity IL-4Rα and either the common γ chain or the IL-13 receptor α chain Binding of IL-4 results in the tyrosine phosphorylation ... autoregulatory or homeostatic mechanism Acting as a decoy to bind and neutralize circulating cytokine, coupled with the high specificity and high affinity of binding for the cytokine, makes the ... phosphorylation of signal transduction molecules including motifs similar to those involved in insulin signaling, the insulin receptor substrate (IRS)-1, IRS2 and signal transducer and activator of transcription...
- 5
- 365
- 0
báo cáo khoa học: " The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis" potx
Ngày tải lên :
11/08/2014, 11:21
... localized to the tonoplast and are proposed to play a role in the efflux of sulfate into the cytoplasm [16] Seeds of the sultr4;1 mutant have an enhanced sulfate content [17] The role of the two ... Altogether, these results bring new insights to the regulation and the function of SULTR1;3 in Pi-deficient plants and identify PHR1 as an important regulator of SULTR1;3 It is interesting to ... level of sulfate and sulfur-containing compounds in various cell types within the roots While the promoter of SULTR2;1 contains a sequence homologous to the PHR1-binding site, induction of Rouached...
- 10
- 427
- 0
Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc
Ngày tải lên :
20/02/2014, 11:20
... lmolÆmin)1Æmg protein)1 The inset in (A) represents the linear transformation of the curve (B) Mg2+ dependency was determined as described in (A) in the presence of increasing concentrations of ... which synthesizes components of the cuticle, may offer a useful target for studies into the mechanism of the development and molting process in the roundworms Investigation into the effects of fluoride ... PPases in the hypodermal cells of sectioned adult worms in immunohistochemical staining), and that the inhibition of PPasecatalyzed PPi hydrolysis by these inhibitors, is likely to prevent the synthesis...
- 13
- 691
- 0
Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
Ngày tải lên :
18/02/2014, 04:20
... well as in the proteolytic processing of cell surface receptors [4,5] MT1-MMP is also involved in the activation of pro-MMP2 and pro-MMP13, leading to the indirect increase in its repertoire of substrates ... sequence for the interaction with the PDZ proteins: Mint-3, AMPA binding protein and glutamate receptor interacting protein (GRIP) It would therefore be interesting to determine whether this motif ... intracellular traffic of MT1-MMP AAA573 to the cell surface It would be interesting to assess the role of GRASP65 with respect to MT1MMP trafficking to the cell surface MT1-MMP, similar to all the other MMPs,...
- 18
- 603
- 0
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
Ngày tải lên :
21/02/2014, 01:21
... The increase in expression levels of galA in the CREA mutant, indicates that at least one of the detected putative CREA binding sites in the promoter of galA is functional Hydrolysis of arabinogalactans ... chains of pectin This is in agreement with a previous study in which the synergy of enzymes degrading the pectin side chains was studied [34] GALA had a positive effect on the activity of the other ... with both the pectinolytic genes encoding main chain cleaving enzymes as well as with the arabinanolytic genes [1] The absence of expression of galA in the presence of pectic galactan indicates...
- 9
- 669
- 0
Báo cáo khoa học: Acylation of lysophosphatidylcholine plays a key role in the response of monocytes to lipopolysaccharide ppt
Ngày tải lên :
31/03/2014, 01:20
... suggests that the signaling pathway from the IFN-c receptor to the acyltransferase involves tyrosine kinases but not protein kinase C Such a finding is not unexpected as the Janus family of cytoplasmic ... (data not shown) The stimulation of LPCAT by IFN-c could be inhibited by the tyrosine kinase inhibitor tyrphostin (2.5 lM) but not by the protein kinase C inhibitor bisindolylmalamide (1 lM) (data ... alter the cell membrane lipid environment so as to favor the assembly of a signaling complex which can then activate the cellular response [15] To elucidate the role of LPCAT in the priming and...
- 7
- 322
- 0
Báo cáo lâm nghiệp: " Sweet chestnut silviculture in an ecological extreme of its range in the west of Spain (Extremadura)" potx
Ngày tải lên :
08/08/2014, 18:22
... (14) complexity of the sumoundings (indicating the degree of simplicity of the orography of the surrounding area; Blanco et al, 1989), (15) wind protection coefficient (according to the fluid mechanics; ... explains more than floristic 70% of the variance, while CV and climatic < set climatic CV2 account together for 75% of the variance, the information being much greater in the latter case (the inertia, ... 1954) (these last seven variables were evaluated by calculating the average of the data of each soil layer, giving each soil layer a weight according to its thickness and to its depth, following...
- 14
- 253
- 0
Báo cáo khoa học: "SemiPro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis" pptx
Ngày tải lên :
09/08/2014, 08:22
... Expression of pro-inflammatory cytokines in the jejunum and colon IL-1b In general, there was weak-moderate IL-1b staining in the jejunal crypts There was predominantly weak staining of the villi The intensity ... changes Therefore the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the intestines during six weeks of fractionated radiotherapy We hypothesise ... although the staining was not considered to be very strong cytokines in the intestines leading to greater tissue damage This study also demonstrated decreased proinflammatory cytokine levels in the intestines...
- 8
- 335
- 0
Báo cáo y học: "Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration" ppt
Ngày tải lên :
09/08/2014, 10:20
... block of tissue (incorporating AF and NP in continuity) was fixed in 10% neutral buffered formalin and processed to paraffin wax Sections were taken for haematoxylin and eosin staining to score the ... decrease in MTL of 0.85 kbp per decade of life in non-degenerate discs (Figure 1a) Interestingly, the MTL differed according to the degree of degeneration in two discs from the same individual ... cells into the degenerate disc to achieve success Competing interests 10 11 The authors declare that they have no competing interests 12 Authors' contributions CLM participated in the design of the...
- 12
- 617
- 0
Báo cáo y học: "Pro/con debate: Octreotide has an important role in the treatment of gastrointestinal bleeding of unknown origin" pptx
Ngày tải lên :
12/08/2014, 23:24
... relevant to current practice considering the proven superiority of protonpump inhibitors [10] The panel of the Nonvariceal Upper GI Bleeding Consensus did not support the routine use of somatostatin ... the initial to 12 hours of the 24 hour intravenous infusions [26] An older, underpowered RCT showed no difference in outcomes between omeprazole and a combination of somatostatin and ranitidine ... since then More contemporary RCTs, totaling 242 patients, have shown no benefits attributable to somatostatin or octreotide, either alone or with ranitidine, compared to the control group administration...
- 4
- 395
- 0
Báo cáo y học: " Beyond the first 25 years: The International AIDS Society and its role in the global response to AIDS" pps
Ngày tải lên :
13/08/2014, 09:20
... any professional society, and the IAS is looking at ways to strategically expand its role in this area in order to strengthen the capacity of the HIV workforce The IAS has been increasingly involved ... activists and others working on the frontlines of the response The IAS encourages all professionals working in the HIV/AIDS field to become members We are convinced that in the struggle against this ... rapidly since its launch in July 2004 It is fully indexed on PubMed and available online [3] The IAS is also increasingly involved in the development and delivery of a range of education and training...
- 3
- 201
- 0
HUMAN CARBOXYLESTERASE 2 SPLICE VARIANTS: EXPRESSION, ACTIVITY, AND ROLE IN THE METABOLISM OF IRINOTECAN AND CAPECITABINE
Ngày tải lên :
24/08/2014, 12:18
... be transcribed into RNA which is then translated into protein A key element of this process is the splicing of premRNA into mature mRNA Splicing is the process whereby non-coding intronic sequences ... glycol The amount of protein was quantified using the Protein the BioRad Protein Assay with bovine serum albumin used for a standard curve The purity of the protein, from the media and the cells, ... There is a higher response rate in solid tumors to irinotecan as compared to other camptothecins This also suggests a role for local, intracellular activation of irinotecan, 16 because the other...
- 130
- 354
- 0
CHARACTERISATION OF GLUTAMYL TRANSPEPTIDASE AND ELUCIDATING ITS ROLES IN THE PATHOGENESIS OF HELICOBACTER PYLORI
Ngày tải lên :
09/09/2015, 17:58
... inducing IL-8 generation in various cell types Analyzing GGT entry into host cells and its probable downstream effects Examining the role of GGT in vacuolation induction Exploring the potential ... the interaction of unphosphorylated CagA with various host proteins For example, CagA binds to the adaptor protein Grb2 which can then lead to a cascade of signalling events resulting in the ... against GGT Determining the subcellular localization of GGT in H pylori Investigating the mechanism by which GGT produces reactive oxygen species (ROS) Assessing the ability of GGT in inducing...
- 281
- 306
- 0
Development of a novel toll like receptor based two hybrid assay for detecting protein protein interactions and its application in the study of CD14 dimerization and FcyRIIA activation
Ngày tải lên :
12/09/2015, 08:20
... domain in its C-terminal portion and a death domain (DD) in its Nterminal portion Activation of TLR or IL-1R recruits MyD88 to the receptor The TIR domain of MyD88 binds to the TIR domains of ... domain-containing adaptors and TLR signaling MyD88 is an essential TIR domain-containing adaptor for the induction of inflammatory cytokines via all the TLRs TIRAP/Mal is a second TIR domain-containing ... sensitive in the detection of protein-protein interactions in transfected yeasts It allows the identification of binding partners for a known protein by expressing the protein in fusion with BD domain...
- 236
- 494
- 0
Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA pDN42 was ... GalNAc-T1, might be involved in the catalytic function of the enzyme To investigate whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 ... amounted to 40% of the parent enzyme activity In a more in- depth evaluation of the function of the three serine mutants, the kinetic properties of the mutant enzymes were compared to those of the...
- 9
- 435
- 0
Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
Ngày tải lên :
30/03/2014, 04:20
... E290) and the S-domain (residues T168 and K169) (Fig 1B) To identify the contact residues involved in the initial binding of Glc to the super-open state (Fig 1C) of this two domain hinge-bending enzyme, ... to the binding of Glc to the super-open conformation have not been identified The characterization of these residues is important for our understanding of how substrate binding is coupled to the ... further supporting the conclusion that more studies are required to settle the issue of a possible MgATP2) induced conformational change in GK Residues involved in the binding of D-glucose to the...
- 15
- 522
- 0
báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin" ppt
Ngày tải lên :
19/06/2014, 22:20
... found in the inflamed meninges and perivascular cuffs of most active MS lesions examined [24] Thus, it would be valuable to further understand the role of DCs within the inflammatory milieu of CNS ... required to migrate This could indicate a possible role for NgR1/2 outside the CNS, perhaps in the activation of DCs or in homing of DCs to specific tissues This is supported by the findings of the ... Journal of Neuroinflammation 2011, 8:113 http://www.jneuroinflammation.com/content/8/1/113 repeat and Ig domain-containing, Nogo receptor-interacting protein) [12] However, due to the findings of...
- 12
- 666
- 0