its oncogenic role in prostate cancer

... Lorand 1989) Interaction with integrins makes it possible for TG2 to be involved in integrin-dependent signal transduction, including in the focal adhesion kinase (FAK) and GTP-binding proteins Raf-1 ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth...

Ngày tải lên: 24/08/2014, 10:58

... denitrifying conditions than Ps aeruginosa NirF also shares 34% sequence similarity with the beta-propeller domain of cd1, indicating a scaffolding role for an intermediate of heme d1 synthesis Roles ... and consumption in Paracoccus pantotrophus strains Starter cultures were grown aerobically in LB with shaking before inoculation of mineral salt medium containing 20 mM nitrate in a 1% v ⁄ v dilution ... similar to that in Asp141 of Met8P The idea of NirF being a dehydrogenase is appealing because of the presence of a putative nucleotide-binding motif in the N-terminal of the protein sequence and...

Ngày tải lên: 15/02/2014, 01:20

... Chapter 24 The Role of PARP Activation in Prostate Cancer 589 Luis A Espinoza VII VIII Contents Section Cell Adhesion Proteins in Prostate Cancer 617 Chapter 25 Integrins in Prostate Cancer Invasion ... of Prostate Cancer 331 Chapter 14 Stem Cells and Prostate Cancer 333 Vildan Bozok Çetintaş, Bur in Tezcanlı Kaymaz and Buket Kosova Chapter 15 Salinomycin-Induced Apoptosis in Human Prostate Cancer ... Prada Section Prostate Cancer Markers Chapter Testosterone Measurement and Prostate Cancer 157 Tine Hajdinjak Chapter Describing Prostate Cancer Dynamics: Second Look at PSADoubling Time and PSA-Specific...

Ngày tải lên: 22/03/2014, 22:20

... GIH cDNA and its role in Vg expression 1.8 1.6 1.4 1.2 0.8 0.6 0.4 0.2 GIH/actin Vg/actin GIH/actin Buffer Vg/actin GIH dsRNA Fig In uence of GIH-dsRNA on Pem-GIH and Vg expression in previtellogenic ... examined for its gonadinhibiting function by using a RNA interference (RNAi) technique RNAi, a post-transcription gene-silencing process in which dsRNA triggers sequence-specific suppression of its ... sequences show the coordinate of nucleotides and amino acids in corresponding lines The full-length cDNA encoding the putative GIH of P monodon was composed of 861 nucleotides containing a 5¢-UTR (93...

Ngày tải lên: 23/03/2014, 07:20

... produced by LNCaP -prostate cancer cell line Prostate cancer cell lines (LNCaP and DU145), as indicated, were maintained in culture as a source of positive-control prostate cancer exosomes (for ... of urine (for at least 18 hours at 37°C) (Figure 6c) Discussion We present the findings of a pilot study, investigating urinary exosomes in prostate cancer patients We had two main aims in the ... loading Bands for prostate- derived proteins PSA or PSMA were evident in patients (p1, p7, p8, p9, p10), indicating that at least some of the exosomes present in the urine were of prostate origin...

Ngày tải lên: 18/06/2014, 15:20

... Against HLA-A24-positive AMACR-positive Prostate Cancer Cell Lines To confirm that CTLs induced with AMACR peptides could exert cytotoxicity against AMACR-expressing prostate cancer cell lines in ... immunohistochemical staining In contrast, PSA was stained in both prostate cancer tissue and non-cancerous tissue (Figure 2C) These data indicated that AMACR had a mostly cancer- specific expression ... Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Surgically resected prostate cancer tissue...

Ngày tải lên: 18/06/2014, 15:20

... replicate in these cells Findings This report builds on and challenges the published data on the absence of A3G protein in prostate epithelial cells lines We demonstrate the presence of A3G in prostate ... cell lines specifically in LNCaP cells [3,18] In addition, the prostate cancer cell line 22Rv1 has been shown to be chronically infected with XMRV and produces highly infectious virus [19] Since ... media containing FBS were added After 1–2 weeks, XMRV infection was confirmed by detecting XMRV p30 protein by using goat polyclonal anti-Rauscher MLV p30 Gag in uninfected (−) and XMRV infected...

Ngày tải lên: 18/06/2014, 18:20

... MDA-7/IL-24 in prostate cancer cells We have stained MDA-7 in prostate cancer cells Shown in Figure 5A, PC-3 wild type cells stained for MDA-7, mostly in the cytosolic region and perinucleus areas ... TGase-4 is linked to the invasiveness of prostate cancer cells [12] and participates in the regulation of the interactions between prostate cancer cells and endothelial cells, the later involving the ... over-expressing TGase-4, staining intensity of MDA-7 is reduced B and D: co-localization of TGase-4 and MDA-7 in normal (B) and tumour (D) prostate tissues TGase-4 staining appears in both stroma and in...

Ngày tải lên: 18/06/2014, 19:20

... MDA-7/IL-24 in prostate cancer cells We have stained MDA-7 in prostate cancer cells Shown in Figure 5A, PC-3 wild type cells stained for MDA-7, mostly in the cytosolic region and perinucleus areas ... TGase-4 is linked to the invasiveness of prostate cancer cells [12] and participates in the regulation of the interactions between prostate cancer cells and endothelial cells, the later involving the ... over-expressing TGase-4, staining intensity of MDA-7 is reduced B and D: co-localization of TGase-4 and MDA-7 in normal (B) and tumour (D) prostate tissues TGase-4 staining appears in both stroma and in...

Ngày tải lên: 20/06/2014, 03:20

... remaining in the upper compartment were completely removed with gentle swabbing The filter was fixed and stained with haematoxylin and eosin The cells invading the lower surface of the filter in ... CD147), matrix metalloproteinase-1 (RA MMP-1), tissue inhibitor of metalloproteinases-1 (RA TIMP-1) and blank contrast (RA blank) in the lining and sublining layers in RA synovium (× 400) The ... normal tissues [25-27] is suggestive of its physiological role in some situations, perhaps in which increased MMP expression is involved in tissue remodeling In RA patients, however, we found that...

Ngày tải lên: 09/08/2014, 06:23

... value concerning the primary detection and the staging of prostate cancer [27] In principle, signal-generation is based on an increased choline metabolism in prostate cancer leading to an increased ... planning in prostate cancer Q J Nucl Med Mol Imag 2009, 53(2):245-268 28 Reske SN, Blumstein NM, Glatting G: [C-11]choline PET/CT imaging in occult local relapse of prostate cancer after radical prostatectomy ... [39] In such a geometrical setting it is defined as: TCP  e  n i SFi i SF i is the surviving fraction within the single subvolume with the running index i (ranging from to m = V/vi) Using the...

Ngày tải lên: 09/08/2014, 08:22

... inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate cancer cell lines Up to now our data revealed that ErPC3 is a potent inhibitor ... lost or inactivated in a variety of solid human tumors, including prostate cancer Increased levels of PIP3 trigger the recruitment of phosphatidylinositide-dependent serine/threonine kinase (PDK1) ... paradigmatic intravenously applicable alkylphosphocholine ErPC3 potently induces apoptosis in prostate cancer cells in vitro These findings corroborate earlier reports on high efficacy of ErPC3 in human...

Ngày tải lên: 09/08/2014, 09:20

... needing knowledge on the combination of underlying biological information The presented results were obtained in a preclinical study in prostate cancer xenografts, and suggest that the combination ... training, testing and validating the network showed curve flattening after a few training epochs, this indicated that no overtraining occurred However, if such a model is to be applied on clinical ... cannula with saline-diluted Gd-DTPA (Magnevist ® , Schering, Berlin, Germany) was inserted into the tail vein Dynamic T1-weighted (T1W) imaging was acquired by performing 20 minutes of dynamic...

Ngày tải lên: 09/08/2014, 09:20

... becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became a major concern in prostate cancer; ... participating in the non-self recognition of prostate cancer cells Thus we can hypothesize, according to results of Hurwitz et al [12] that the enhanced immune function, involving resting and terminally ... of life in men being treated for prostate cancer with radiotherapy: instrument validation and its relation to patient-assessed bother of symptoms Urology 1999, 53:359-366 Fiorino C, Sanguineti...

Ngày tải lên: 09/08/2014, 09:20

... FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein expression ... Saitoh JI, Takahashi T, Mitsuhashi N: Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival ... anti -prostate cancer activity in vitro and in vivo Clin Cancer Res 2004, 10:5282-5292 Ouchi H, Ishiguro H, Ikeda N, Hori M, Kubota Y, Uemura H: Genistein induces cell growth inhibition in prostate...

Ngày tải lên: 09/08/2014, 09:22

... Kemmerling L, Born EJ, Mini R, Greiner RH, Aebersold DM: Daily organ tracking in intensitymodulated radiotherapy of prostate cancer using an electronic portal imaging device with a dose saving acquisition ... summarized in Table Incidence of single symptoms (dysuria, incontinence, retention, frequency/urgency, hematuria) as well as the highest toxicity occurred in a given patient concluding all single ... rectum and bulb of the penis using MRI delineation for radiotherapy of the prostate Int J Radiat Oncol Biol Phys 2003, 57:1269-79 Sobin LH, Wittekind CH: The prostate In TNM Classification of Malignant...

Ngày tải lên: 09/08/2014, 09:22

... performed on-line by repositioning the target according to the internal markers, moving the treatment couch manually To calculate the minimum required margin width around the clinical target ... 90%inthe median values (lines percentile 75% values10%variability, prostate line), format, Measured deviations in and Measured deviations in LR, SI and AP directions in a box plot format, showing ... the interobserver variability is low Therefore implanted markers and EPID based methods are used for targeting in radiotherapy of prostate cancer with increasing frequency Our results provide information...

Ngày tải lên: 09/08/2014, 09:22

... participated in study design, data interpretation, manuscript drafting and revising for intellectual content SZ participated in study design, data interpretation and manuscript revising for intellectual ... Bolognesi A, Cattaneo GM, Calandrino R: Intraand inter-observer variability in contouring prostate and seminal vesicles: implications for conformal treatment planning Radiother Oncol 1998, 47:285-292 ... Shinohara N, Harabayashi T, Miyasaka K: Threedimensional intrafractional movement of prostate measured during real-time tumor-tracking radiotherapy in supine and prone treatment positions Int...

Ngày tải lên: 09/08/2014, 10:21