isolation purification and culture of murine spermatogonial stem cells

... tissue-specific stem cells and decreases their regenerative ability We therefore examined whether aging affected the quantity and quality of cardiac stem cells using cells obtained from 26 patients of various ... right atria and cultured cardiosphere-derived cells (CDCs), which are a type of cardiac stem cell Then we investigated growth rate, senescence, DNA damage, and the growth factor production of CDCs ... autologous stem cell transplantation in elderly patients Resident cardiac stem cells exist in adult human hearts and inherently mediate cardiogenesis and angiogenesis1–3 Recently, cardiac stem cells

Ngày tải lên: 04/12/2022, 14:53

... increased self-renewal of organ and embryonic stem cells and the serum-free establishment of human organoids from healthy and diseased intestine and liver Moreover, the lipophilicity of Wnt3a protein ... of adult stem cells and offer advantages for all cell culture uses of Wnt3a protein Department of Cell Biology, Erasmus University Medical Center, PO Box 2040, Rotterdam 3000 CA, The Netherlands ... concentrations of Wnt3a protein and offers advantages for all tissue culture uses of Wnt3a protein This is demonstrated by the considerably improved performance of Wnt3a liposomes in embryonic stem cell culture

Ngày tải lên: 04/12/2022, 15:06

... Molecular and proteomic characterization of human mesenchymal stem cells derived from amniotic fluid: comparison to bone marrow mesenchymal stem cells Stem Cells Dev 2007, 16:931–952 Page of 10 19 ... http://www.translational-medicine.com/content/12/1/56 Page of 10 Figure Timing of adherence and confluence of primary cultured cells In 10% aPRP, MNCs adhered the soonest, and the adherence time gradually increased as the concentration of aPRP ... isolation of mesenchymal stem cells from umbilical cord blood Stem Cells 2004, 22:625–634 Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH: Isolation of multipotent mesenchymal stem cells from umbilical

Ngày tải lên: 14/12/2017, 16:57

... stages of liver stem cells which have the capacity for unlimited proliferation and mutilineage differentiation, and such kind of liver stem cells change their expression markers in dependence of differentiation ... hepatoblast-like cells To characterize the cells cultured under the influence of 10 µM SB431542 in combination with Y27632 (0, 1, 2, 5, 10 µM), the and EpCAM, and moderately for Dlk1 and ICAM AFP-positive cells ... of HLPCs may exist and have a variety of property in different conditions Culture conditions determined in this study were able to culture types of hPSC-HLPCs characterized as HSC-like cells and

Ngày tải lên: 04/12/2022, 15:13

... densities of 106, 105, 104, and 103 cells per 100 μl PBS by using the right and left sides of the same mouse Similarly, the mice in group and were injected with primary cells cultured in Medium M and ... Figure Tumorigenicity of primary culture cells of breast tumors in mouse models (A-B) Tumorigenesis comparison among cells cultured in Medium M and Medium DB and BCC lines (C) and (D): tthe tumor ... the isolation and propagation of human breast cancer cells from primary tumour biopsies Biomedical Research And Therapy, 2(2): 207-219 219 Isolation and propagation of human breast cancer cells

Ngày tải lên: 16/12/2017, 03:41

... Sharma et al., (2013), Chand and Chandra (2014), Deep et al., (2014), Taware et al., (2014) and Koley and Mahapatra (2015) Effect of culture media on growth and sporulation of Alternaria brassicae ... Journal of Plant., 3(2): 356-360 How to cite this article: Valvi, H.T, J.J Kadam and Bangar, V.R 2019 Isolation, Pathogenicity and Effect of Different Culture Media on Growth and Sporulation of Alternaria ... Growth and sporulation of A brassicae were studied in vitro using eight synthetic and nonsynthetic culture media The data from Table 1, PLATE-IV and Figure revealed that of the eight culture

Ngày tải lên: 09/01/2020, 17:13

... concentration of 20 μg/ml, measured at a frequency of 500 MHz at a temperature of 70 oC RESULTS AND DISCUSSION 3.1 Isolation and purification Figure Fractionation of glycosaminoglycans from body wall of ... pattern of sulfation of N-Acetyl-Galactosamine and fucose residues IR and NMR spectra of two frations showed that sulfate groups were primarily occupied at C4 of pyranose residues in F2 and C6, C2 and/ or ... Suzuki, K., 206 Isolation, purification and structural characteristics of glycosaminoglycans from sea Masuda, H., Kyogashima, M., and Ishii, T - Isolation and partial characterization of fucan sulfateses

Ngày tải lên: 28/06/2021, 15:16

... in the absence of MEFs, and the gene expression profile of MEFs interferes with those of ES cells and embryoid bodies (EBs) Even 9-day-old EBs are still contaminated by MEFs, and MEF-specific ... (EGFP) expression of single α-MHC+ cells with different morphologies (subpanels a, c, and e) Detection of α-cardiac actinin (subpanels b, d, and f) and connexion43 (subpanels g and h) was performed ... heart failure [1,2] Recently, genetic strategies for differentiation of stem cells and nonmuscle cells through expression of developmental control genes that specify cardiac cell identity have

Ngày tải lên: 14/08/2014, 20:22

... embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent cells Conferral of ... B.L Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture Cell 70, 841-7 (1992) Kanatsu-Shinohara, M et al Generation of pluripotent stem cells from neonatal ... Oct4 in the reprogramming of murine somatic cells to pluripotent cells Cell Stem Cell 6, 167-74 (2010) Kim, J.B et al Pluripotent stem cells induced from adult neural stem cells by reprogramming

Ngày tải lên: 09/09/2015, 18:56

... embryonic stem cells (hESCs) derived from isolation and serial sub -culture of ICMs from 5-day old human blastocysts hold the promise of revolutionizing the future of medicine by the creation of early ... efficient and safe xenofree cryopreservation method for the storage of human embryonic stem cells Stem Cells 22: 779-89 Richards M, Tan SP, Tan JH, Chan WK, Bongso A (2004) The transcriptome profile of ... WK and Bongso A (2005) A Cluster Analyses of the SAGE expression profiles of Human Embryonic Stem Cells indicate possible role of POU5F1 in cell cycle progression and identifies a robust set of

Ngày tải lên: 16/09/2015, 15:55

... Soleimani,M., and Nadri,S (2009) A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow Nat Protoc 4, 102-105 Sommer,L., and Rao,M (2002) Neural stem cells and regulation of ... apoptosis Unlike other cells, the cell cycle of stem cells is characterized by a very short G1 phase and a high proportion of cells in S-phase (White and Dalton, 2005) However, during stem cell differentiation, ... 3.3.2 Knockdown of ERp29 decreased the expression of Pae3 and Par6 48 3.4 Knockdown of ERp29 increased apoptosis of BMSCs … 51 3.5 Knockdown of ERp29 decreased proliferation of BMSCs ……………

Ngày tải lên: 02/10/2015, 17:15

... 1979, Ng and Idler reported the presence of two types of gonadotropin in plaice and flounder pituitaries Subsequently, two different forms of GtH, GtH-I and GtH-II, were isolated from salmon and shown ... sensitization of rat pituitary cells to gonadotropin-releasing hormone: involvement of protein kinase C- and calcium-dependent signaling pathways Endocrinology 139:3796-3802 Croft, L., Schandroff, S., ... The studies of the amino acid (Itoh et al., 1988) and the cDNA sequences (Sekine et al., 1989) revealed close similarity of GtH-I and GtH-II to the mammalian FSH and LH, respectively, and thus established

Ngày tải lên: 08/11/2015, 17:15

... crassa Fungal culture 2.2.1 Mycelia Culture 2.2.2 Conidia Culture 32 33 ii CONTENTS 2.3 PAGE Isolation of N crassa Proteins 2.3.1 Isolation of Mycelia Proteins 2.3.2 Isolation of Conidia Proteins ... and inhibited by nicotinamide (Pekala and Anderson, 1978; Yost and Anderson, 1981; Kim et al., 1993) Most mammalian NADase activity is associated with the membrane and. .. transfer of ... Cycling Assay 81 Discussions 4.1 Isolation and Characterization of Conidial NADase from N crassa 83 4.2 Identification of NADase Sequence 85 4.3 Cloning and Expression of Gi|85106032 86 4.4 NAD+ Cycling

Ngày tải lên: 08/11/2015, 17:15

... in uninduced murine ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We ... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time...

Ngày tải lên: 07/03/2014, 12:20

... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout (IFN-gR KO) mice were cultured ... Materials and methods Isolation and expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1...

Ngày tải lên: 12/08/2014, 11:23

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... trace of total soyasaponin and soyasapogenol A and B extracts 38 Fig DNA cell-cycle histograms of control (untreated) cells and TS-, SG-A-, and SG-B-treated cells for 24, 48, and ... ISOLATION, PURIFICATION AND DETECTION OF SOYASAPONINS AND THEIR ASSOCIATED BIOACTIVITIES IN CULTURED HEPATOCARCINOMA CELLS ZHANG WEI MPA (MSC), MUST; APPLY...

Ngày tải lên: 11/09/2015, 10:06

... cell culture, the attached cells stretched and took the shape of a typical spindle-shaped fibroblast phenotype These adherent cells could be readily expanded in vitro by successive cycles of trypsinization, ... of cell therapies Semin Thorac Cardiovasc Surg 2008, 20:94-101 Chen L, Tredget EE, Wu PY, Wu Y: Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and ... hypoxia stress, cells viability was detected by MTT assay and flow cytometry measurement, respectively Cells (1 × 104 cells) were cultured in serum-free medium for 24 h with 0.01 μmol/L of simvastatin,...

Ngày tải lên: 10/08/2014, 05:21

... the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C -cells of the thyroid as well as ... Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells ... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of...

Ngày tải lên: 14/08/2014, 08:20

... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...

Ngày tải lên: 25/10/2013, 22:20

... effects of MSCs in vitro mediator of the cells paracrine anti-fibrotic effects These findings help to improve our understanding of the cellular and molecular basis of MSCs’ anti-inflammatory and paracrine ... presence and absence of IL-10 *P < 0.05 and **P < 0.01 versus Cont group (D) Representative western blots for collagen I and III in the presence and absence of 0.1 lM angiotensin II and IL-10 ... Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan...

Ngày tải lên: 18/02/2014, 04:20