isolation purification and culture of murine spermatogonial stem cells

Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... in uninduced murine ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human ... nature and extent of covalent histone modiﬁcations and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We ... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time...

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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Ngày tải lên : 12/08/2014, 11:23

... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout (IFN-gR KO) mice were cultured ... Materials and methods Isolation and expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1...

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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Ngày tải lên : 11/09/2015, 10:06

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... trace of total soyasaponin and soyasapogenol A and B extracts 38 Fig DNA cell-cycle histograms of control (untreated) cells and TS-, SG-A-, and SG-B-treated cells for 24, 48, and ... ISOLATION, PURIFICATION AND DETECTION OF SOYASAPONINS AND THEIR ASSOCIATED BIOACTIVITIES IN CULTURED HEPATOCARCINOMA CELLS ZHANG WEI MPA (MSC), MUST; APPLY...

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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf

Ngày tải lên : 18/06/2014, 15:20

... http://www.translational-medicine.com/content/7/1/40 Q-Banding Cells in log phase were cultured with 50 μL of colchicine for hours and mitotic cells were gently blown with a pipette Cells were centrifuged at 235 × g for 10 minutes and the ... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... endotoxin and exert cytotoxic effects on primary cultures with an increase of lipidic intracellular droplets [29] Liberase is a blend of highly purified enzymes used to improve the isolation and cultures...

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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx

Ngày tải lên : 10/08/2014, 05:21

... cell culture, the attached cells stretched and took the shape of a typical spindle-shaped fibroblast phenotype These adherent cells could be readily expanded in vitro by successive cycles of trypsinization, ... of cell therapies Semin Thorac Cardiovasc Surg 2008, 20:94-101 Chen L, Tredget EE, Wu PY, Wu Y: Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and ... hypoxia stress, cells viability was detected by MTT assay and flow cytometry measurement, respectively Cells (1 × 104 cells) were cultured in serum-free medium for 24 h with 0.01 μmol/L of simvastatin,...

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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt

Ngày tải lên : 14/08/2014, 08:20

... the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C -cells of the thyroid as well as ... Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells ... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of...

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Purification and cloning of PCR products

Ngày tải lên : 25/10/2013, 22:20

... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

Ngày tải lên : 18/02/2014, 04:20

... effects of MSCs in vitro mediator of the cells paracrine anti-fibrotic effects These findings help to improve our understanding of the cellular and molecular basis of MSCs’ anti-inflammatory and paracrine ... presence and absence of IL-10 *P < 0.05 and **P < 0.01 versus Cont group (D) Representative western blots for collagen I and III in the presence and absence of 0.1 lM angiotensin II and IL-10 ... Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan...

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the dialects and culture of the kinki region - kinki japanese

Ngày tải lên : 27/03/2014, 12:26

... Asi iN -U 橋 d h ensa oH-O -AYU 篭車一 F-u Uk ル KU ー K-s Utu b-U uS cuUk h a n E anD? k-S uTU B-u Us CUu H - ・ka N e And? 月 R 靴ぶす rn a I cny ad big rde cosik hptcs dg o tI rn a g o mrig o d onn ... a δi ， l s ocsoal，o h u o t i rvr w e h dos o of r o h c r w o ie y u hne f o l e h n e rp y u f o t t l k h gvs o cag.Iy u i e e v rt i ae i Koof g aot aig o o r rgt otd o Jpns c s s n yt ，o ... te ci n a s ses ts i Nvrhls，ery vr aaya ad fie ay n s k ad eetees nal eeyslrmn n ofc ld i O a a n truhu asihsland osek tnad aaee o ue n hogotKna a ere t pa sadr Jpns f s i r bsns，lhuh e r rnnito...

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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt

Ngày tải lên : 30/03/2014, 13:20

... Enzyme activities of COS-7 cells, transfected with the short and long splice variants of mouse fucokinase (mFK) and human fucokinase (hFK, AJ441184) (B) Fucokinase activities of HeLa cells transfected ... expression levels of pyrophosphorylase and long and short splice variants of fucokinase Primers and probes were designed using the PRIMEREXPRESS program (Version 1, PE Applied Biosystems, Foster ... amounts of the three mRNAs analyzed were based on standard curves (Applied Biosystems User Bulletin 2) prepared by a serial dilution of control cDNA Results Cloning of putative mouse L-fucokinase and...

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temple university press customizing the body the art and culture of tattooing mar 2008

Ngày tải lên : 11/06/2014, 15:14

... (Laumann and Derick, 2006) The movement of tattooing into the realm of popular culture displays certain features of the contemporary culture industry and reveals how fad-like phenomena emerge Culture ... art, prison art, tiki art, anime and manga, pulp art, and tattooing Inspired by the dadaists and surrealists of the 1920s and 1930s, advocates and practitioners of lowbrow art reject the constraints ... provide an account of the changes that have occurred within the artistic and cultural world of tattooing and the larger world of body alteration in the past decade and a half One change and a fairly...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression of EB signature...

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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot

Ngày tải lên : 20/06/2014, 23:20

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc

Ngày tải lên : 07/08/2014, 20:23

... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...

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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx

Ngày tải lên : 08/08/2014, 17:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult-derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the functioning, and physioand ... regeneration and repair [43] Systemic injection provides a model of choice for delivering adult derived-neural progenitor and stem cells for the treatment of neurological diseases and injuries,...

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Báo cáo y học: "The P2X7 receptor is a candidate product of murine and human lupus susceptibility loci: a hypothesis and comparison of murine allelic products" potx

Ngày tải lên : 09/08/2014, 06:22

... P2X7-dependent exposure of PS on New Zealand Black (NZB) lymphocytes, New Zealand White (NZW) lymphocytes and (NZB × NZW)F1 (NZB/W) lymphocytes To enable the direct comparison of responses of cells from NZB ... P2X7-stimulated shedding of CD62L by lymphocytes To enable the direct comparison of responses of cells from New Zealand Black (NZB) mice, lymphocytes New Zealand White (NZW) mice and (NZB × NZW)F1 (NZB/W) ... flow cytometric and IL-1β secretion experiments, and wrote the first draft of the manuscript JHM designed allele-specific primers and typed the P2RX7 genes of NZB mice and NZW mice, and 16 17 Tsao...

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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx

Ngày tải lên : 09/08/2014, 10:20

... the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and ... involvement of Wnts is complicated by their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of ... is the homing of stem cells to sites of injury and subsequent wound healing Although some tissue repair may be accomplished by the division of indigenous differentiated cells, such cells are most...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in ... space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells...

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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Ngày tải lên : 10/08/2014, 05:21

... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... acetylation of histone H4; H3K4me1/2/3, (mono-, di- and tri) methylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone ... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine...

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