isolation of viruses in cell cultures

... may be ‘trapped’ in chronicity by an incapacitating sick role The lingering effects of ‘institutionalism’ among discharged patients of that era offer indirect corroboration (Wing and Brown, 1970) ... of evidence inconveniently lying elsewhere The rumour of recovery from schizophrenia in other cultures made innocent rounds in early ethnographic circles only to be roundly discredited in clinical ... odds of recovery ratio of roughly 1.5 favouring the non-industrial group During this period, too, independent reports from other ‘developing’ regions continued to appear, employing a host of methods...

Ngày tải lên: 01/11/2013, 09:20

... aphasia in 31% in Ibadan and 38% in Berlin; sexual dysfunction in 45% in Ibadan and 80% in Berlin; and post stroke emotional disorder in 75% in Ibadan and 68% in Berlin In the subset of five respondents ... validity, internal consistency reliability and factorial validity The grouping of items of HRQOL measures into domains makes analysis and interpretation easier Similarly, this grouping of domains into ... Journal of Alternative Medicine Research 2009, 1:383-390 Owolabi MO: Comparative analysis of the seed of life model: Examining the mind-brain puzzle and programming Journal of Alternative Medicine...

Ngày tải lên: 12/08/2014, 00:20

... expression of some cell cycle related genes In a single cell, the amplitude and phase of a cell cycle related gene are considered two independent parameters in a sinusoidal model Within a cell population, ... the number of time points in exp2 One often neglected but important factor in interpreting and analyzing cell cycle related gene expression data is the quality of synchrony of the cell culture ... true of the estimated ampli- In this paper, we have analyzed the effect of the scattering of phase angles of a cell cycle related gene in a cell population on the amplitude of expression of this...

Ngày tải lên: 13/08/2014, 23:20

... amplitudes in two different experiments In Table they are trying to explain differences between different experiments in terms of variation in the phase angles of peaking of gene expression in different ... Wolfinger the Replotting of the K values of [1] two experiments analyzed Replotting of the K values of the two experiments analyzed by Liu, Gaido and Wolfinger [1] was a sinusoidal expression in ... determining something about cells For example, "different conditions" means growing cells in one case at 25°C and in another case at 37°C and seeing what happens to them or what is different in...

Ngày tải lên: 13/08/2014, 23:20

... sparked initial curiosity about viruses in the environment Since then, the literature has reflected an explosion of interest in the prevalence of viruses in various environments and the effects of ... capable of producing the toxin and increasing their virulence Equally compelling evidence of lysogenic conversion outside of the marine venue was provided in a recent study on the contribution of ... host VIRUSES IN SOILS Historical overview In contrast to growing knowledge regarding the viral ecology of marine ecosystems, the ecological role of viruses in soil microbial communities remains...

Ngày tải lên: 13/11/2014, 10:53

... domain is crucial for the activation of STAT proteins Typically, the SH2 domain plays an important role in mediating protein-protein interaction by binding to specific phospho-tyrosine containing ... functional domains with Stat3 as an example The N-terminal domain is involved in stabilising the interaction with two STAT dimers during tetramersiation The coiled-coil domain is involved in interaction ... report indicating that point mutations in the linker domain of Stat1 results in the abolishment of its transcriptional activity following IFN-γ induction (Yang et al., 1999) 1.4.5 The SH2 domain...

Ngày tải lên: 11/09/2015, 16:06

... involved in the control of developmental timing in C elegans uncovered a ~22nucleotide single-stranded non-coding RNA as the product of the lin-4 gene Lin-4 RNA repressed the protein levels of ... Screening of predicted targets of miR-210 59 4.5 Screening of predicted targets of miR-193a 63 Discussion 67 5.1 Differential expression of miRNAs during cell cycle phases 67 5.2 Role of miR-210 in ... of the phase the cell has 4n chromosomes The cell then continues into the G2 phase and is growing to prepare for cell division During mitosis (M phase), the cell seperates into two daughter cells...

Ngày tải lên: 05/10/2015, 22:31

... involved in the control of developmental timing in C elegans uncovered a ~22nucleotide single-stranded non-coding RNA as the product of the lin-4 gene Lin-4 RNA repressed the protein levels of ... Screening of predicted targets of miR-210 59 4.5 Screening of predicted targets of miR-193a 63 Discussion 67 5.1 Differential expression of miRNAs during cell cycle phases 67 5.2 Role of miR-210 in ... of the phase the cell has 4n chromosomes The cell then continues into the G2 phase and is growing to prepare for cell division During mitosis (M phase), the cell seperates into two daughter cells...

Ngày tải lên: 06/10/2015, 20:35

... postoperative insulin resistance Insulin resistance in type diabetes Tyrosine phosphorylation of the insulin receptor increased over the same concentration range of insulin in cells from patients ... recovery cells were incubated with indicated concentration of insulin for 10 Mean ± SE, n ¼ subjects In this and the following figures, the insulin-stimulated effect was obtained by setting the ... incubation of the cells (Fig 3) Maximal insulinstimulated increase in tyrosine phosphorylation of the insulin receptor was 10.6 ± 2.3 and 9.6 ± 4.2-fold (n ¼ 5) directly and after overnight incubation,...

Ngày tải lên: 07/03/2014, 16:20

... assays following the method of Bradford (1976) using bovine serum albumin as a standard The final protein content of the intracellular fraction in 100 ml of liquid medium was mg Preparation of mycelial ... spruce contains more isorhapontin than astringin (Fig 3) The rapid decline of the levels of glycosides was described in in vitro maintained excised bark discs of Sitka spruce following the fungal ... decrease in concentrations of stilbene glycosides The decrease in isorhapontin (occurring in the highest concentrations in embryogenic cell cultures) , astringin and piceid levels was observed in WF-elicited...

Ngày tải lên: 07/08/2014, 10:21

... precursor cells [34] but may also appear in cultured epithelial cells [35] To distinguish between different populations of cells, double labeling of cellular cytokeratin and vimentin was performed Cells ... number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not altered in ... showing distinct sensitivity Thus, the impact of a-amylase on cell growth in vitro depends on cellular conditions, origin, e.g rat strain, and distinct cellular characteristics The rat primary cells...

Ngày tải lên: 10/08/2014, 10:21

... Live/Dead staining, a method involving rinsing of cell cultures, mainly measured firmly attached cell attachment and viability These results emphasize the importance of consideration of the type of assay ... effect of Cd2+ ion on cell detachment is more prominent than that on cell viability Determination of effect of different media and acid treatment on size and integrity of in- house synthesized QDs In ... toxicity of QDs in Caco-2 cell cultures, treated QDs were diluted to working concentrations in cell culture medium (pH 7.4) and incubated with Caco-2 cells cultured in a 96-well plate for 24 hours in...

Ngày tải lên: 11/08/2014, 00:22

... and maintaining all transduced cell lines AK and RD were instrumental in providing support to the research in the form of both instruction in and preparation of all lentivirus vectors used in this ... transduced cells were maintained in complete L-15 with 35 μg/mL hygromycin (Invitrogen) For infection of cells, complete L-15 without any antibiotics was used Cells were incubated at 28°C without CO2 Infection ... appear in the control lanes lacking hRz TCID50 immunofluorescence assays We determined the effectiveness of each ribozyme in suppressing overall infectious virus production using an immunoflourescence-based...

Ngày tải lên: 12/08/2014, 04:21

... of phosphatases from inactivation [26] Inhibitor of serine-threonine phosphatases [27] Inhibitor of tyrosine phosphatases [26] Broad range inhibitor of serine-threonine kinases Inhibits binding ... combination of inhibitors of protein kinases to assess possible involvement of these kinases in UV-B signaling pathway leading to catharanthine accumulation The C roseus cells were treated with inhibitors ... dimers by coupling catharanthine obtained from cell cultures with vindoline obtained from the cultivated plants The production of catharanthine by C roseus cell cultures has been one of the most...

Ngày tải lên: 12/08/2014, 05:20

... gradient in Arabidopsis pistils was implicated in the misguidance of pollen tubes suggesting a role for GABA in intercellular signaling [43] β-Alanine is a non-protein amino acid synthesized mainly ... increased in abundance later than most amino acids The concentration of asparagine also increased in Pseudomonas syringae-infected tomato leaves, suggesting that asparagine, and not glutamine is ... tobacco cell cultures, PLA2 was also induced in response to elicitor treatment [29] In opium poppy cells, only Ophosphocholine increased in response to elicitor treatment The first step in choline...

Ngày tải lên: 12/08/2014, 05:20

... 0.5 μg/mL epinephrine, 6.5 ng/mL triiodothyronine, μg/mL insulin, ng/mL retinoic acid, 10 μg/mL transferrin, and 0.001% gentamycin (v/v) 16HBE14o- cells were maintained in Dubelco's Minimum Essential ... of small airway epithelial cells Lineage Lineage verification of small airway epithelial cells A & B; Gene expression of CCSP (CC-10) in small airway (SAEC) cell cultures, large airway cell cultures ... Figure Morphology of epithelial cells is maintained over passage Morphology of epithelial cells is maintained over passage Phase contrast micrographs showing no morphological variation in bronchial...

Ngày tải lên: 12/08/2014, 14:20

... IFN-γ inducing cytokine, which induced secretion of endogenous IFN-γ in A549/PBMC co -cultures due to a specific interaction between lymphocytes and A549 cells IL-12 also induced an increase in IP-10 ... the cell type in PBMCs interacting with the A549 cell line, secretion of IFN-γ was studied in lymphocyte/A549 and monocyte/A549 co -cultures As shown in Table 1., lymphocytes exclusively interact ... amounts of leucocytes in lung tissue in COPD patients interact with several cell types including lung epithelial cells as well as endothelial cells in a similar manner increasing IP-10 secretion...

Ngày tải lên: 12/08/2014, 15:21

... expression of an E2F variant containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing ... for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins Mdm2 (as well as c-Abl) binds to the C-domain All pocket proteins ... encoding a pRBbinding protein with properties of the transcription factor E2F Cell 70:337–350 Helin K, Harlow E, Fattaey A (1993) Inhibition of E2F-1 transactivation by direct binding of the retinoblastoma...

Ngày tải lên: 25/10/2013, 21:20

... of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ginseng roots ... Suspended cells of P vietnamensis were initiated through callus induction from the cultivated plant root [5] The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric ... suspension cultures of P vietnamensis for biomass and production of ginseng saponin (secondary metabolite) The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial sucrose...

Ngày tải lên: 12/02/2014, 10:20