isolation of phages from natural sources

... throughout the project Professors and staffs of the Department of Organic Chemistry and Faculty of Chemical Engineering for their help and useful advice My friends in the Laboratory of Organic Chemistry ... medicine, treatment of cessation of breathing for newborn babies and as an antidote against the depression of breathing by overdoses of heroin Caffeine was found in tea with a content of 3-4 % Tea has ... Vdd= volume of filtrate after extracting mt= mass of green tea • Standard curves of caffeine (using UV-VIS method) A=absorption C=concentration of caffeine (ppm) 81 • Standard curves of EGCG (using

Ngày tải lên: 13/02/2021, 08:48

... throughout the project Professors and staffs of the Department of Organic Chemistry and Faculty of Chemical Engineering for their help and useful advice My friends in the Laboratory of Organic Chemistry ... medicine, treatment of cessation of breathing for newborn babies and as an antidote against the depression of breathing by overdoses of heroin Caffeine was found in tea with a content of 3-4 % Tea has ... Vdd= volume of filtrate after extracting mt= mass of green tea • Standard curves of caffeine (using UV-VIS method) A=absorption C=concentration of caffeine (ppm) 81 • Standard curves of EGCG (using

Ngày tải lên: 04/04/2021, 00:00

... Structures of sesquiterpene lactones 75 3.4.2 Skeletal structures of flavonoids, isoflavonoids and neoflavonoids 76 3.4.3 Skeletal structures of subgroups of flavonoids 76 HMBC and. .. of ... Chapter Isolation and characterization of components from ethyl acetate fraction of Ixeris sonchifolia Hance 48 55 3.1 Introduction 56 3.2 Materials and methods 56 3.2.1 Isolation of the ... Effects of fractions to isolated from Ixeris sonchifolia Hance extract on the viability of H2O2-exposed PC12 cells 42 2.3.3 Evaluation of antioxidant activities of fractions to isolated from Ixeris

Ngày tải lên: 11/09/2015, 10:06

... view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvularia allergens, and of the ... to the varying amounts of allergenic and nonallergenic proteins. Moreover, natural extracts had various drawbacks such as chances of contamination from other allergen sources being prone to proteolysis, ... mechanism of allergy (Adapted from Holt et al.,1999) 23 Chapter Figure 1.2: Factors responsible for atopy (adapted from Umetsu et al., 2002) Figure 1.3: Progression of allergic sensitization from

Ngày tải lên: 14/09/2015, 08:47

... approach is one of the most effective approaches towards large scale expressed proteome profiling. In this technology, a library of directionally cloned partial DNA sequences from randomly selected ... sequences from the cDNAs corresponding majority of the mRNAs help in elucidating the primary structure of the expressed proteins (Yamamoto and Sasaki, 1997). Moreover, identification of differentially ... regulation and modifications of extracellular proteins (Oda et al., 2006). In this approach, a 1D or 2D SDS PAGE of the total protein is used to generate a proteomic profile of the organism. These

Ngày tải lên: 14/09/2015, 09:09

... proofreading activity. A 50µl reaction consisted of 0.5µl of each primer (100µM), 5µl of Expand Long Template Buffer 1, 2µl of dNTP (10µM), 3µl of cDNA and 1µl of Expand Long Template Enzyme mix containing ... 6µl of sterile water, out of which 1µl was used as the template in the PCR. A ten-reaction master mix consisted of 10µl of 10X Buffer, 2µl of primers specific for the Ek/LIC Vector, 2µl of dNTP ... First three letters are taken from genus (Cur from Curvularia) followed by the first letter of the species (l from lunata) followed by a unique Arabic number. Lengths of the allergen sequences are

Ngày tải lên: 14/09/2015, 09:10

... dust as well. A total of 12 samples from bedroom floors, samples from bedroom carpets, 13 samples from living room floors, 17 samples from living room carpets and samples from kitchen floors were ... from indoor environments in Singapore Levels of SOD allergen were tested... The concentrations of the individual allergens were expressed as ng/m3/week of dust after extrapolating from ... standardization of allergen extracts by aiding in proper quantitation of allergens by comparing the strength of reactivity to the source, in order to administer proper amount of allergen. Hence,

Ngày tải lên: 14/09/2015, 09:15

... different degrees of acetylation depending on their sources of origin and mode of isolation The average degree of acetylation (DA) of chitin samples was determined from data of elemental analysis ... composition of raw material of crustacean shells Chitin was isolated from six sources, two kinds of marine shrimp shells, crab female and crab male shells, cuttlefish pens and lobster shells, all from ... of internal deacetylation units resonate at 3.4 ppm The absence of methyl proton resonance from protein between 1.0 and 1.5 in 1H NMR spectra of chitin gives a good indication of the purity of

Ngày tải lên: 25/08/2016, 11:21

... CO2-rich natural gas reservoirs will become a source of raw material for the petrochemical industry Through conversion of natural gas into syngas, a range of important chemicals can be produced, of ... by the Ministry of Natural Resources and Environment in 2014, in the period 1994 - 2010 total greenhouse gas emissions in Vietnam increased rapidly from 103.8 million metric tons of carbon dioxide ... a wide range of chemicals Potential utilisation and conversion of CO2-rich natural gas Syngas, or synthesis gas, is the mixture comprising of hydrogen, carbon monoxide and very often some carbon

Ngày tải lên: 12/01/2020, 00:26

... searches of the natural environment for particular species of algae and other microorganisms of interest to a broad range of laboratories around the world Page 10 of 12 of fluorescent versions of VHHs ... Chlamydomonas cells Colorimetric analysis of the effects of duration of incubation on the progression of binding of VHH B11 (blue line) at a concentration of 20 nM to living C reinhardtii cells ... protection from numerous biotic and abiotic environmental factors In the case of eukaryotic algae, relatively little is known of the composition, structure or mechanisms of assembly of cell walls

Ngày tải lên: 27/05/2020, 01:17

... sensitivity pattern of L monocytogenes from different samples of cattle sources was carried out in Aizawl district of Mizoram It is mainly a hilly state of North-eastern region It extends from 21°56'N ... 2829-2843 Table.1 Distribution of different samples of cattle sources collected from Aizawl district of Mizoram Sl No State Aizawl (Mizoram) Type of sample Number of samples Cattle faeces Raw cow ... wise prevalence of L monocytogenes isolated from different samples of cattle source from Aizawl (Mizoram) district Sl No State Type of sample Number of sample tested in winter Number of sample tested

Ngày tải lên: 09/07/2020, 01:35

... 2186-2208 Source of organism From farm Leaf surface imprints PPFMs bacterial colonies appear Purification of bacterial colony Plate.1 Isolation of phyllospheric methylotrophic bacteria from rice leaf ... leaves of field-grown crops averaged about 106 cfu of PPFMs per leaflet and typically >80% of the viable bacteria recovered from leaves were PPFMs (Omer et al., 2004) Materials and Methods Isolation ... the features of the leaf print (Plate.1) Total 33 isolates were isolated from phyllosphere of paddy crop grown at different locations of middle Gujarat agro climatic zone III Out of 33 isolates,

Ngày tải lên: 27/09/2020, 13:22

... was employed for isolation of actinomycetes (Table-2) Isolation of actinomycetes is the first and the most important step of actinomycetes resource development The primary aim of such investigations ... division of outside sources Various media and methods were employed for the isolation of actinomycetes, in order to standardize the isolation procedure few preliminary investigations such of media ... were inhibited by isolates (27%) The zone of inhibition in case of gram+ve bacteria ranges from 1.0 mm to 15mm and in case of gram–ve bacteria ranges from 1.0 mm to 11 mm Among the tested gram+ve

Ngày tải lên: 28/06/2021, 09:36

... fucans or fucoidans from the body wall of the Vietnamese sea cucumber S horrens Glycosaminoglycans from different species of holothuria vary in pattern of sulfation, presence of branching, and ... glycosaminoglycans from sea cucumber S horrens * Isolation of glycosaminoglycans: glycosaminoglycans were isolated from the body wall of sea cucumber S horrens using a modification of a previously ... 206 Isolation, purification and structural characteristics of glycosaminoglycans from sea Masuda, H., Kyogashima, M., and Ishii, T - Isolation and partial characterization of fucan sulfateses from

Ngày tải lên: 28/06/2021, 15:16

... The results of the isolation showed that 35 strains of aerobic bacteria 44 Isolation of marine bacteria from sponges in the South-central coastal region of Viet Nam … were isolated from 20 sponge ... about 59.79% of bacterial strains isolated from sponge showed alginate lyase activity [16] The content of alginate from brown seaweed, especially the 20-40% high from S mcclurei from which the ... [10] Alginate from S mcclurei was extracted as previously described in [9] 2.2 Sample collection 42 Isolation of marine bacteria from sponges in the South-central coastal region of Viet Nam …

Ngày tải lên: 28/06/2021, 15:47

... continuous process of trial and error, and only by accepting the possibility of failure can a scientist make progress [22] Thus, this study offers an overview of retraction offered from various perspectives, ... 1955 Change of venue and the conflict of laws The University of Chicago Law Review , 22(405) (University of Chicago Law School) October 1, 1966 October 1, 1959 On the primary site of nuclear RNA ... Characteristics of retracted articles based on retraction data Table Publishers with the largest number of retracted papers Total no of retracted papers No of journals with retractions No of fields

Ngày tải lên: 17/10/2022, 15:51

... of essential oils from different parts of plants (leaf, root and citrus peel) on the properties of fish gelatin film Thus, the objective of this study was to comparatively study the effects of ... found on the surface of films containing essential oils in spite of high amount of oil added Addition of all types of essential oils led to the change in mechanical properties of films Films with ... uniformity of film network formation (Pe´roval et al 2002) Therefore, essential oils from different sources affected the mechanical property of film differently Water vapour permeability WVP of films

Ngày tải lên: 02/11/2022, 08:50

... conditions for the successful isolation and initial characterization of urinary exosomal miRNA from human urine As a proof of principle, we also examined urine samples from individuals with nephrotic ... best exosome isolation methods for proteomic analysis and RNA profiling[17] However, to the best of our knowledge, there have been no in-depth studies investigating the isolation of urinary exosomal ... centrifugation (A) Electron micrograph of a urinary exosome (B) Histogram of the exosome diameter Size analysis of 10 fields of view showing that 92.7% of the exosomes were between 30-120nm http://www.ijbs.com

Ngày tải lên: 04/12/2022, 15:14

... °C for further processing RNA isolation was carried out Isolation and characterization of microglia from postmortem CNS tissue The isolation of viable microglia from post-mortem human CNS tissue ... microglia Since the development of our procedure for the isolation of human microglia in 2012 [25], we performed microglia isolations from over a hundred brain donors from the Netherlands Brain Bank ... from WM of MS donors (Fig 4a) CD11b expression was also elevated in microglia from WM of MS donors, but did not reach significance (p = 0.067) The same analysis of CD45 and CD11b expression of

Ngày tải lên: 04/12/2022, 15:15

... Therefore, screening for strong bioflocculants from natural sources is needed. Journal of Water and Environment Technology, Vol.3, No.1, 2005 - 146 - Two types of microbial flocculants exist: ... disruption is not effective for the isolation of bioflocculants. Thus, NaOH extraction was the most effective method for the extraction of bioflocculants from activated sludge, followed by steaming ... ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid. In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide corresponded to that of

Ngày tải lên: 05/09/2013, 09:08