isolation of headspace volatile compounds from samples

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... coefficient K of the target compounds The K values were determined by GC-MS analysis as follows: One drop (about mg) of the essential oil was added to the mixture of equal volume of the upper ... temperature Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase...

Ngày tải lên: 07/03/2014, 21:20

... potential (m3/mg of waste), Mi = the mass of solid waste in the ith section of the landfill (mg), ti = the age of the ith section of the section of the landfill (years), t = the age of the landfill ... Journal of Energy and Environment (IJEE), Volume 4, Issue 1, 2013, pp.85-92 Figure Graph of frequency against range of values of age of landfill (t) Figure represents range of values of the total ... toxicity from methane, carbon dioxide, and volatile organic compounds respectively The generation of methane and carbon dioxide from landfills has been well studied, and a very large body of literature...

Ngày tải lên: 05/09/2013, 16:11

... efficiency of C pneumoniae DNA extraction Sufficient recovery of final PCR product has been also seen when protein A from Staph aureus has been used for isolation of C pneumoniae from whole serum ... fluorescence microscope at x1350 magnification DNA isolation DNA isolation from whole serum Briefly, 1.0 ml of whole serum was mixed with 0.5 ml of lysis buffer (0.2 M Tris-HCl buffer, pH 7,2 supplemented ... µl of DNA was added to reaction mixture containing 1x PCR buffer (Silex, Moscow, RF) containing 10 mM Tris-HCl, pH 8,3 , 2.5 mM MgCl2, 15 pmol of each primer, 200 µM of each of dNTPs and U of...

Ngày tải lên: 26/10/2012, 08:57

... Change of 2-AP and Other Volatile Compounds in Processing Bun from Rice 3.4 Investigating the changes of 2-AP and other volatile compounds of rice in Bun processing Table The volatile compounds ... Quantification of 2-AP in pandan leaf: Fig System of the SPME extraction 732 Content of - AP in pandan leaf extracted by SDE was calculated as follows: Research on the Change of 2-AP and Other Volatile Compounds ... and collidine as internal standard Duration of extraction was 30 minutes from apparition of the first drop of water in the bottom of the condensed tube Volatile compound extracts were then concentrated...

Ngày tải lên: 28/08/2013, 16:28

... Change of 2-AP and Other Volatile Compounds in Processing Bun from Rice 3.4 Investigating the changes of 2-AP and other volatile compounds of rice in Bun processing Table The volatile compounds ... Quantification of 2-AP in pandan leaf: Fig System of the SPME extraction 732 Content of - AP in pandan leaf extracted by SDE was calculated as follows: Research on the Change of 2-AP and Other Volatile Compounds ... and collidine as internal standard Duration of extraction was 30 minutes from apparition of the first drop of water in the bottom of the condensed tube Volatile compound extracts were then concentrated...

Ngày tải lên: 19/03/2014, 16:20

... threshold of volatiles, synergistic effect between volatiles and the interaction of non -volatile with volatile flavour compounds (Nisperos-Carriedo and 21 Shaw, 1990) The volatile compounds present ... Diagram for the isolation of headspace flavour compounds of orange juice by SPME (Jia et al., 1998) Figure 5.1 59 Chromatogram (top) and aromagram (below) of aroma active compounds of Pontianak ... Chemical composition 61 3.4.2 Volatile compounds in citrus juices 63 References 75 CHAPTER 4: CHARACTERIZATION OF VOLATILE COMPOUNDS IN PEEL OIL OF SELECTED CITRUS FRUITS FROM ASIA 80 4.1 Abstract...

Ngày tải lên: 11/09/2015, 09:11

... from a pair of samples The 27 pairs of samples and the compounds found in each pair resulted in 192 pairs of data for the extractable organic compounds Effects of purging on nonvolatile compounds ... Henry's Law constant Nonvolatile Organic Data The effect of purging on nonvolatile compounds also was tested statistically Data for nonvolatile compounds in 27 pairs of samples collected before ... AND DISCUSSION Volatile Organic Compound Data A total of 302 samples were analyzed for up to 19 volatile organic compounds Concentrations of all VOCs were anomalously low in two samples, which...

Ngày tải lên: 05/03/2014, 21:20

... properties to Program Offices (e.g., Office of Water, Office of Solid Waste and Emergency Response, Office of Prevention, Pesticides and Toxic Substances) and Regional Offices Also, SPARC has been used ... number of molecular structures The values of these latter parameters, in most instances, must be derived from measurements or from the expert judgment of specialists in that particular area of chemistry ... interactions of the P with the two states δrespKa describes the change in the delocalization of π electrons of the two states due to P This delocalization of π electrons is assumed to be into or out of...

Ngày tải lên: 22/03/2014, 14:20

... IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band (indicated ... isolation of large amounts of pure Ndh complex from maize chloroplasts for further structural and functional studies This may include identification of further Ndh subunits, as well as determination of ... containing peptides of Ndh subunits were dried and used for MALDI-TOF-MS in reflective mode MALDI-TOF-MS analysis of Ndh peptides The measurements were carried out on a Reflex III TOF system (Bruker...

Ngày tải lên: 23/03/2014, 13:20

... three-dimensional structures of certain domains of nitric oxide synthase isoforms are known [53±57], we are not aware of the three-dimensional structure of the calmodulin binding sites of any isoform being reported ... spectrum) Data from Y + fragmentations give sequence information from the N-terminal end of the peptide (see schematic arrows below the spectrum) For a review of fragmentations of MH+ ions of peptides ... 82±101 of caveolin-1 in the form of glutathione S-transferase fusion proteins have been shown to attenuate the activities of all three isoforms of NOS [62] A novel 10-kDa protein inhibitor of nNOS,...

Ngày tải lên: 31/03/2014, 15:20

... catalog of GS–MS (Table 4) We estimated the most of abundant volatile compounds and their % area of mushrooms in the chromatogram were as follows: first flush of flavor or aroma volatiles of L edodes ... are of desirable taste and aroma One of the objectives of this research is the determination of volatiles of L edodes and Pleurotus spp (Oyster mushrooms) by estimating with the identification of ... Eicosamethylcyclodecasiloxane a 4.32 % Area of volatile compounds in the chromatogram K and Na contents of L edodes tent to increase after the first flush stage Average levels of K and Na of L edodes were 2348.24...

Ngày tải lên: 28/06/2014, 11:20

... extract fatty acid from the bacterial cell-wall and identified with microbial identification system (MIDI, Inc., USA) Isolation of bacteria: One hundred strains of LAB were isolated from selective ... broth without bacterial culture Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic ... In vitro cholesterol-lowering test: Among isolated 100 strains of LAB, of Streptococcus strains, 11 of Lactobacillus strains and of Bifidobacterium strains were selected as provisional probiotic...

Ngày tải lên: 07/08/2014, 18:20

... numerator is the number of samples in which a particular species of FN was detected (+) and the denominator is the total number of samples tested cFour OA samples and no RA samples were subjected ... species of SF FN were typically detected in samples from both types of patient: a ~200+-kDa cluster of staining, corresponding to the ~200+-kDa species in 1D electrophoresis; a series of In SF samples ... blot analysis of unconcentrated synovial fluid samples in the absence of primary mAbs (not shown) (a) Schematic diagram of the typical 2D migration of three predominant species of synovial fluid...

Ngày tải lên: 09/08/2014, 01:23

... Figure cells from a capable of AML but not normal cells Peptides3arepatient withincreasing the cloning efficiency of Peptides are capable of increasing the cloning efficiency of cells from a patient ... probability of a biologic response of AML patients to The probability of a biologic response of AML patients to each peptide Cells from different AML specimens were incubated in the presence of peptides ... dose of 10-4 M or GM-CSF at 100 U/ml without addition of fresh medium or peptide The solvent used for peptide preparation served as a control Following 14 days of incubation, the number of col- Isolation...

Ngày tải lên: 10/08/2014, 22:20

... curves of the bands were processed with the aid of specific MediaCybernetics, Gel-Pro Analyzer Version 6.0 software Molecular cloning of the 2.2 kpb dsRNA from B cinerea CCg378 The dsRNAs of B ... CCg378 may correspond to or be part of the genome of a partitivirus [16], whereas those of the THg324 and SUg275 strains may correspond to the genome of members of the Hypoviridae and Totiviridae ... different samples of dsRNA from Saccharomyces cerevisiae 1743 (for details see Binding capacity of dsRNA molecules by CF11 cellulose in Results and Discussion); lane 4, empty; lane 5, nucleic acids of...

Ngày tải lên: 11/08/2014, 21:21

... isolated from mosquitoes collected in these areas [12,13] Molecular epidemiological study showed that all of the JEV isolates obtained either from mosquitoes or from clinical samples of human ... first time from CSF samples of JE patients in China Previously, partial sequences of JEV GI were detected in specimens of meningitis patients in Japan [14] and in JE patients in an outbreak of China ... HM366548-HM366552] amplified from CSF samples are clustered within GI (Figure 1) by phylogenetic analysis based on the 240 nucleotide (nt) sequence of the JEV prM gene Aliquots of CSF samples which showed...

Ngày tải lên: 12/08/2014, 02:20

... species [15] Most of the surveillance studies [3,4,7,8] have focused on the detection of AIVs from waterfowl and shorebirds while only a few reports are available on isolation of AIV from raptors ... the first report on isolation of AIV in a Bald eagle from the U.S The bald eagle in question was recovered from Gloucester County, Virginia in March, 2008 In general, samples from injured raptors ... Institutes of Health; rRT- Authors’ contributions PTR was responsible for collection of samples from bald eagles SMG and SS were responsible for overall coordination and planning of the study...

Ngày tải lên: 12/08/2014, 04:20

... http://www.virologyj.com/content/7/1/189 Page of Figure Map of Kerala showing the location of sample collection areas classical symptoms of CHIK [15] Samples were obtained from the outpatient department of three Primary ... genotype of the CHIKV (Figure 3b) The gene sequences of CHIKV obtained from mosquito and patient samples formed a close cluster, distinct from the strains isolated previously from Kerala [14], rest of ... for sequencing reactions Five clinical samples each from Olavanna, Beypore and Chaliyum were used, making a total of 15 samples Selected regions of the Page of CHIKV genome (nucleotide position,...

Ngày tải lên: 12/08/2014, 04:20

... microscopy of MPs from the sputum of a CF patient Multiple spherical particles, ranging from 100 to 500 nm, are visualized Page of * A) 7.5 50 25 stable PCD CF B) Figure Quantification of MPs in ... the first time, of the presence of MPs in sputa obtained from CF patients The membrane composition of MPs reflects the plasma membrane of the original cell at the precise moment of MPs production ... 10 μl of supernatant MPs were incubated with 10 μl of specific antibody (1 μg/ml; FITC-conjugated; BioLegend, San Diego, CA) After 15 of incubation at + 4°C, samples were diluted in 500 μl of Porro...

Ngày tải lên: 12/08/2014, 11:22

... the saline of some of the samples to a final concentration of 1% in the agar-gels and the incubation saline About 500 ml of crushed ice was added to some of the jars after the removal of the agar-gels ... removing the gels, the samples were mixed in large buckets (one with normal samples and one with gentamycin samples) , whereafter each of a series of 500 ml subsamples of saline with larvae was ... possible effect of gentamycin on migration of lung larvae out of the agar-gels (see Table 5) Gentamycin was added to some of the lung samples as described for Exp After incubation of the lung agar-gels...

Ngày tải lên: 12/08/2014, 15:20