isolation of cardiac myocytes and fibroblasts from neonatal rat pups

... curves of the bands were processed with the aid of specific MediaCybernetics, Gel-Pro Analyzer Version 6.0 software Molecular cloning of the 2.2 kpb dsRNA from B cinerea CCg378 The dsRNAs of B ... profile of the CCg378 strain revealed the presence of four dsRNA bands with approximate molecular sizes of 2.2, 1.95, 1.75 and 1.4 kilobase pairs (kbp) (Figure 2, lane 1), whereas the THg324 and ... recombinant plasmid of about 5.2 kbp linearized with NcoI is shown Treatment with NcoI and SpeI generated two bands, a corresponding to the cDNA of about 2.2 kbp and the Page of other to the linear...

Ngày tải lên: 11/08/2014, 21:21

... 1: Effect of blending time and gentamycin This experiment was designed to examine the rate of migration out of liver agargels (1, 2, and h), the effect of blending time and the effect of adding ... Acta vet scand vol 42 no 2, 2001 Isolation of migrating Ascaris suum larvae 281 Table Impact of incubation time of agar-gels, sedimentation time in incubation jars, and addition of ice and 1% glucose ... the first hours of incubation, and the migration rate seemed independent of blending time and addition of gentamycin Table shows that cooling with ice (reduced the temperature of the saline with...

Ngày tải lên: 12/08/2014, 15:20

... and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L migratoria ... of primary amino acid structures of EcR and USP from L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of ... constant, KD, of PonA was calculated from the saturation curve of the specific binding and the Scatchard plot (Fig 5) The KD values of LdEcR-A and LdEcR-A ⁄ LdUSP calculated from saturation curves...

Ngày tải lên: 07/03/2014, 21:20

... Optimization of FOG concentration Five FOG concentrations were tested to find out the optimal substrate concentration for lipolytic activity This range of concentration was a common range of FOG concentration ... Addition of antifoam and surfactant was studied The optimal concentration of antifoam (CF-18) and surfactant (PVA) was found as 0.4%w/v and 0.2 %w/v respectively 10% w/v of straw in the best ration of ... initial pH, growth temperature, and type and substrate concentration In this paper the optimal temperature and pH were investigated as shown by other reports (Stams A.G etc 1997 and Jaeger, K.E etc...

Ngày tải lên: 22/03/2014, 09:20

... protein from D desulfuricans [10] MATERIALS AND METHODS Preparation and estimation of ¯avodoxin and apo¯avodoxin Flavodoxin from D vulgaris was obtained as the recombinant protein that was puri®ed from ... is the mean activity of the salt, and LU and LF are the number of salt ions bound by the unfolded and folded states of the apoprotein, respectively Determination of kinetic and thermodynamic constants ... complete dissociation occurs; and xe is the concentration of FMN and apoprotein at equilibrium Solution of Eqn (7) between xi (initial), x and ti, t, yields the integrated rate law for a ®rst-order,...

Ngày tải lên: 31/03/2014, 15:20

... morphologies of the samples prepared in aqueous solutions of equimolar of ZnCl2–HMT mixture with and without 50 vol.% EG additive The initial concentrations of Zn2? were 0.01 M, 0.05 M, and 0.1 M, ... respectively The morphology of the product changed greatly depending on EG additive and concentration of Zn2? In the absence of EG, at such low initial ZnCl2– HMT concentration of 0.01 M, the product ... those of Fig 3a, c A strong band due to deformation vibration of H2O molecules at 1,630 cm-1, an absorption band centered at 3,434 cm-1 represented the characteristic of an O–H stretching vibration,...

Ngày tải lên: 22/06/2014, 01:20

... pathological aspects of cardiac and smooth muscle cell functions, while Part III consists of articles concerning factors influencing structure and function of cardiac and smooth muscle cells and tissues ... (magnification, 2500X), and activated them with ATP Figure Relation between the total incident electron dose and the survival rate of muscle myofibrils, expressed as percentage of myofibrils contracted ... lattice of actin and myosin filaments, and that muscle contraction results from relative sliding between actin and myosin filaments (Fig 6) Figure Electron micrographs of longitudinal thin section of...

Ngày tải lên: 28/06/2014, 15:20

... end-systolic and end-diastolic phase images were displayed (Figs and 2), and EDV, ESV, EF, SV, CO, and myocardial mass were calculated automatically (Tables and 2) The EDV and ESV were 48.80 ± 23.30 ml and ... capability of both technologies for the visualization of the coronary lumen and the detec- tion of coronary artery stenosis The sensitivity of MDCT for the detection of significant stenosis ranges from ... pigs and micropigs (Fig 3) Discussion Cardiac mortality is closely related to cardiac volumes and global left ventricular function, which is expressed as the LVEF [36] An accurate assessment of...

Ngày tải lên: 07/08/2014, 20:23

... quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248-254 (1984) H+ translocating ATPases of the plasma membrane and tonoplast of plant ... high specificities for ATP, with a K value of 0.32 mM m Quenching of ACMA (initial rate: 500%) was collapsed by nigericin and inhibited by N03 K value of pumping: 0.67 mM m mM) induced total inhibition ... (Tables I and II) Based on these properties, the low density membrane ATPase may be of tonoplast origin and the other of plasma membrane origin The + PPase activity (stimulated by K and inhib-...

Ngày tải lên: 09/08/2014, 04:20

... repeating on–off CCO input signal and is generated by accumulating the temperature change for each on and each off segment of the input signal (Fig 1) Calculation of RVEF is based on estimation of the ... this setting of preoperative cardiac surgery, characterized by preoperative fasting, diuretic therapy, and the vasodilatory effect of sevoflurane, relative hypovolemia is common and could account ... level of the right ventricle To reflect sudden changes in CO, the Vigilance Monitor provides a STAT mode of operation, which has been shown to permit accurate measurement of CCO [24] The software...

Ngày tải lên: 12/08/2014, 22:21

... of the experiment, and, consequently, the median duration and rate of the noradrenaline infusion were significantly lower (duration: 111 (0 to 282) versus 752 (531 to 935) minutes; infusion rate: ... decreased heart rate and cardiac output without affecting myocardial relaxation, and significantly decreased troponin I blood levels; increased the rate of direct, aerobic glucose oxidation, and reduced ... positive end expiratory pressure (PEEP) 10 cmH2O, inspiratory-toexpiratory (I/E) ratio 1:1.5, respiratory rate adjusted to partial pressure of arterial carbon dioxide (PaCO2) 35 to 45 mmHg (but...

Ngày tải lên: 13/08/2014, 18:22

... inoculums of 37,500 mg VSS/L granular sludge was added followed by the mixture of pig manure and glycerol and DI water with COD:TKN ratio of 40:1 and HRT of 50 days corresponding to OLR of 0.16 ... was left for separation and the glycerol layer at the bottom was separated 2.2 Substrate preparation Fresh pig manure was collected from Faculty of Natural Resource, Prince of Songkla University, ... Standard test method for determination of glycerin assay by titration 2010 [17] Hautfenne, A International union of pure and applied chemistry Standard methods for the analysis of oils, fats and...

Ngày tải lên: 09/09/2015, 10:17

... fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m th filter bank St ( m ) ... noise free and noisy conditions Noise is additive white Gaussian at a 10dB signal-to-noise-ratio 46 Time waveforms and respective spectrograms of Disgust and Fear emotions of Burmese and Mandarin ... faster heart rate, pulse rate, respiration rate There is also, shunting of the blood to more vital areas, and release of blood sugar, lactic acid and other chemicals, all of which is involved in...

Ngày tải lên: 15/09/2015, 22:03

... A Plan of Complexes II and III and Tomb from Lago dell’Accesa Area B Hypothetical plan of Complexes VII and VIII from Lago dell’Accesa Area A Hypothetical plan of Complexes III and IV from Lago ... reconstruction of the Upper Building at Poggio Civitate Plan and section of Complex X from Lago dell’Accesa Area A Plan and section of Complex III from Lago dell’Accesa Area A Section of a wall footing from ... theories must recognise and provide ways for understanding the processes of reuse and abandonment before and after deposition in the record The factors of reuse and abandonment greatly affect...

Ngày tải lên: 18/11/2015, 09:59

... regulation of c-jun expression in quiescent and proliferating rat liver The role of the )148 to )124 region of c-jun in transcriptional regulation of c-jun in rat liver is established and two factors, ... orchestrated regenerative response, activating a cascade of cell signalling events necessary for cell cycle progression and proliferation of hepatocytes Because progression of liver proliferation ... determination of optimum concentrations of monovalent and divalent cations (A) Differential complex formation of nRNE-d and rRNE-d with Jun-25 EMSA reactions were carried out in the presence of ng of radiolabelled...

Ngày tải lên: 16/03/2014, 18:20

... among the most studied member of the family and has been used in many studies for augmentation of bone and bone-tendon junction regeneration [3,4] Because of the role of BMP-2 in bone formation, ... [15] and muscle ossification [16] BMP-4 and -7 and TGF-beta1 were also reported to be involved in the initiation and development of ossification of spinal ligaments (OSL) [17] Activities of BMPs ... and week (all p > 0.05) (Figure 2B) Discussion The pathogenesis of calcific tendinopathy, including the cause of tendon pain, tendon degeneration and calcification, remained largely unknown and...

Ngày tải lên: 20/06/2014, 04:20

... ,gnitnuom erofeB )ASU ,rotceV( etartsbus enidiznebonimaid a htiw depoleved saw noitcaer esadixorep ehT )ASU ,rotceV( tik etilE eht gnisu sexelpmoc esadixorep nitoib-nidiva fo noitamrof yb dewollof ,ydobitna ... eht nI )sworra ,C( 1-niloevac rof evitisoponummi erew sllec noilgnag emoS )A( reyal gnitimil lanretxe dna ,reyal mrofixelp retuo ,reyal raelcun renni ,sreyal mrofixelp renni eht ni deniats ylesuffid ... htiw Co4 ta thginrevo rof detabucni neht dna mures esroh lamron %01 ot desopxe erew snoitces eht ,SBP ni sehsaw eerht retfA ytivitca esadixorep suonegodne kcolb ot nim 02 rof retaw dellitsid ni...

Ngày tải lên: 07/08/2014, 18:21

... 4, and for control rats on days 0, 10, 21, and 28; and 9, 2, and for arthritic rats on days 10, 21, and 28 For gene expression analysis, on days 0, 10, 21, and 28, a volume of 3–5 ml blood from ... transcript profiling of peripheral circulating lymphocytes As an initial proof -of- concept, we demonstrated the feasibility of such technology by successful profiling PBMCs in a rat CIA model ... plasma from CIA rats as well as in supernatants from cultured arthritic rat synovial tissue Further characterization of the genes novel to arthritis will advance our understanding of and facilitate...

Ngày tải lên: 09/08/2014, 07:20

... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... area, and their ratio, as index of complexity Statistical analysis Data were expressed as mean ± standard deviation (SD) or median and range A comparison of the morphometric data obtained from ... obtained from CD patients and those from control subjects All the cultured cells were FBs with normal karyotype, as demonstrated by the FSP positivity, cytokeratine 20 negativity, and the Q banding...

Ngày tải lên: 18/06/2014, 15:20

... high-activity D-hydantoinase from Jannaschia sp CCS1 Y Cai et al Table Substrate specificity of HYDJs The relative rate of hydrolysis of various substrates is shown as a percentage of the rate at which HYDJs ... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... the substrate binding pocket, Phe63, Leu92, Phe150 and Tyr153, were revealed to be related to substrate binding and recognition of d-p-HPH and l-p-HPH by HYDJs, preferring d-p-HPH as substrate The...

Ngày tải lên: 18/02/2014, 08:20