isolation northern blot hybridization and real time pcr
Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
Ngày tải lên :
09/08/2014, 10:22
... edema and erythema limited to the foot or ankle, = slight edema and erythema from the ankle to the tarsal bone, = moderate edema and erythema from the ankle to the tarsal bone, and = edema and ... experimental work, and advised on the study S-HP, and J-KM are the senior researchers, and they supervised the experimental work and advised on the study All authors read and approved the final ... cDNA was amplified by PCR using IDO sense (5'-CACTGTACCAGTGCAGTAG-3') and antisense (5'-ACCATTCACACACT CGTTAT-3') primers, and using Foxp3 sense (5'-CAGCTGCCTACAGTGCCCCTAG-3') and antisense (5'CATTTGCCACGAGTGGGTAG-3')...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
Ngày tải lên :
18/06/2014, 15:20
... WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Figure 1B, 2A, and 5A) Fusions of DCs to the HCC cells coexpressed the HCC cells-derived WT1 antigens and ... autologous FCs nine times Autologous HCC cells expressed high levels of WT1 and HLA-ABC (HLA-A2+/A24-) and low levels of CEA but not HLA-DR, costimulatory molecules (CD80 and CD86), and maturation ... contributions SK, SH, and EH conceived of the study, participated in its design, coordination, and preparation of this manuscript SK, MM, AT, EN, and, YN prepared reagents and PBMCs SH, HK, and YS prepared...
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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
Ngày tải lên :
10/08/2014, 10:21
... BglIIand SalI xperiments in this figure and following figures were performed at least three times on separate occasions (B) IDO gene integration and transcription by PCR and RT -PCR (C) Western blot ... after co-culture was detected by RT -PCR, Real- time PCR or Western blot (A) Analysis of RT -PCR products of Foxp3 and comparison of the gray scale value between Foxp3 and b-actin by agarose gel electrophoresis ... real- time RT -PCR analysis of Foxp3 gene expression Foxp3 gene expression was detected in CD3+T cells after days of co-culture with IDO+ CHO cells by quantitative RT -PCR analysis CD3+T cells and...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
Ngày tải lên :
21/02/2014, 01:21
... 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshly prepared and used immediately Effect of various compounds on the enzyme activity The effect of metal salts, and chelating and sulfhydryl ... Japan) Organism and growth conditions Strain 10d was obtained from farm soil in Hyogo Prefecture, Japan The basal medium containing 4-amino-3-hydroxybenzoic acid used for the isolation and cultivation ... 0.4 g yeast extract and deionized water in L total volume, with the final pH adjusted to pH 6.8 Solution B contained g MgSO4Æ7H2O, and mg each of CaCl2Æ2H2O, CuSO4Æ5H2O, ZnCl2, and FeSO4Æ7H2O, with...
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Báo cáo khoa học: The role of residue Thr249 in modulating the catalytic efficiency and substrate specificity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 ppt
Ngày tải lên :
30/03/2014, 10:20
... no effect on the kcat values on catechol and 3-MC (Table 1), whereas mutations T249A and T249G reduce by four times the kcat values on catechol and 20 times those measured on 3-MC (Table 1) Thr249 ... ECDs belonging to subfamilies and 6, such as DHpCD and THTD, catalyze the transformation of 3,6-disubstituted and 4,5-disubstituted catechols, respectively [24–26], and exhibit high regioselectivity ... C2,3O were determined on purified 3,5-DMC and 3,6-DMC (Table 1) The Km values were found to be 74 lm and 21 lm, respectively, which are approximately 50 and 14 times higher than that measured on catechol...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
Ngày tải lên :
18/06/2014, 15:20
... American Type Culture Collection (ATCC) and maintained in Iscove's Modified Dulbecco's Medium supplemented with glutamine, and penicillin and streptomycin (100 IU/ml and 100 μg/ml, respectively, Sigma-Aldrich, ... prelabelled) with 100,000 PBMCs and incubated for h Cells were stained with FITC-conjugated Annexin V and ToPro3 (Sigma Aldrich, UK) and cells that were PKH26+, annexin high and ToPro3 high were regarded ... who had been vaccinated on to occasions (Mean = 4) with DCT and DCTI pulsed with hTERT peptides Figure 3A and 3B show the time course and flow cytometry plots for a patient (N001) who developed...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
Ngày tải lên :
09/08/2014, 01:23
... About 5–10% of CD4+ T cells and less than 1% of CD8+ peripheral T cells constitutively express CD25 in normal naïve mice, and such cells are found in the CD5high and CD45RBlow T cell fractions ... CCR5 and their human counterparts expressing CCR4 and CCR8 [19,20] Such a distinctive pattern of chemokine receptors suggests that TR cells might be rapidly recruited to sites of inflammation and ... phenotype of TR and primed T cells, but they are more relative than absolute For example, although both primed T cells and TR cells express CD25, the latter does so to a higher level and more stably...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
Ngày tải lên :
09/08/2014, 01:24
... liver, the lung, and the intestine, and they appear to function via IL10 secretion [32,38–40] Wakkach and colleagues [41] recently identified a subset of dendritic cells in the spleen and lymph node ... the danger has passed, and to retain tolerance to self probably combines many mechanisms both centrally and in the periphery Our understanding of regulation is now expanding with the identification ... exposure to antigen and costimulation These cells proliferate and become CD4 T effectors Activation also induces a subset of CD4+CD25– T cells to upregulate CD25 and FoxP3 and acquire CD25+CD4+...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
Ngày tải lên :
09/08/2014, 03:24
... Differential expression of Fas and Fas ligand in acute and chronic graftversus-host disease: up-regulation of Fas and Fas ligand requires CD8+ T cell activation and IFN-gamma production J Immunol ... effects include upregulation of CD25, CTLA-4 and CD40 ligand expression on CD4+ cells [14,47], and increased tumor necrosis factor-α production by both CD4+ and CD8+ cells [47] The TGF-β costimulated ... CD8+ and CD4+ T cells that produce immunosuppressive cytokines have been described Those that produce predominantly TGF-β and variable amounts of IL-10 and IL-4 have been called Th3-type cells, and...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
Ngày tải lên :
09/08/2014, 06:22
... streptavidin-allophycocyanine and streptavidin-PE, and matched antibody pairs for ELISPOT analysis of IFN-γ (R4-6A2 and biotinylated XMG1.2) and IL4 (BVD4 1D11 and biotinylated BVD6-24G2) production ... hybridoma and purified the monoclonal antibodies from the supernatant; planned and conducted all animal experiments, including ELISA and ELISPOT analysis; and drafted the manuscript PKP and MG scored ... intravenously, and 24 hours later mice were killed and the distribution of radioactivity in various organs and the rest of the body was measured in a γ-counter (Wallac Counter, Turku, Finnland) Alternatively,...
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Báo cáo y học: "β Transforming growth factor-β-induced regulatory T cells referee inflammatory and autoimmune diseases" potx
Ngày tải lên :
09/08/2014, 06:22
... important new evidence documents that Treg can be expanded and/ or induced de novo from CD4+CD25– precursor T cells How, where, and if the size and function of this population can be intentionally ... intraperitoneal (ip) injection with HDM on days and and then challenged by intratracheal (it) injection on days 14 and 21 were injected intraperitoneally on days and 14 with Treg Three days after the second ... naive and memory T cell proliferation and can be expanded in vitro without loss of function J Exp Med 2001, 193:1295-1302 Dieckmann D, Plottner H, Berchtold S, Berger T, Schuler G: Ex vivo isolation...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
Ngày tải lên :
09/08/2014, 06:23
... generated and expanded in vitro, might tip the balance and allow a true curative action in animal models of T1D [12], and much work is now directed to this area [20] Two recent studies in T1D and experimental ... withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of fully functional CD4+CD25+ Treg, RA is still not cured These strands of evidence seem to ... series of studies in RA and other chronic autoimmune joint inflammatory diseases such as juvenile idiopathic arthritis and spondyloarthropathies, in which phenotype, distribution and functional studies...
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Báo cáo y học: "Regulatory T cells in systemic lupus erythematosus: past, present and future" pot
Ngày tải lên :
09/08/2014, 13:22
... [80], TNF family proteins [81], and adenosine and its receptors [82,83] downhill and fatal, whereas human disease is cyclic and characterized by exacerbations and remission Moreover when patients ... Similarities and differences between Foxp3+ nTregs and Foxp3+ iTregs are reviewed elsewhere [15] Importantly, both IL-2 and TGFβ are required for the maintenance of Foxp3 expression and for the ... immunogenic and tolerogenic DCs, and thereby to correct Treg numbers and function In active lupus, the products of the apoptotic cells that bind to Toll-like receptor 7, Toll-like receptor 8, and Toll-like...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
Ngày tải lên :
11/08/2014, 21:21
... with FITC-anti-E and PE-anti-CD11c and analyzed by FACS respectively (D) The infected bmDCs and the spDCs from challenged mice were collected times at day 1, and 5, and a real- time PCR was performed ... vitro and in vivo (A) The in vitro infected bmDCs and the spDCs from P3-challenged mice were harvested and analyzed with RT -PCR Bands shown are 467-bp PCR products specific for JEV (B) The bmDCs and ... were harvested and used to detect the viral production by RT -PCR and Western blotting and the samples were subjected to PCR The consensus primers 5’-GCTCTGAAAGGCACAACC-3’ (primer1) and 5’-CTGAAGGCATCACCAAAC-3’...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
Ngày tải lên :
12/08/2014, 13:22
... experiments JCZ and XL were responsible for flow cytometry and data collection CW and SMQ analyzed the data JBX and HZS drafted the manuscript YZJ, QZ, JCZ and XL read, critically revised and all authors ... generation and differentiation of Tregs or /and Th17 cells in MPE To evaluate the contribution of cytokines to the numbers of pleural CD39+Tregs and Th17 cells, we purified naïve CD4+ T cells from MPE and ... cytometry CD4 + T cells were also stained with CD4-PerCPCy5.5, CD25-PE, and CD39-FITC, (eBiosciences), and CD4+CD25highCD39+ T cells and CD4+CD25- responder T cells were sorted using a Beckman Coulter...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
Ngày tải lên :
13/08/2014, 09:21
... expression and concomitant glucose uptake in CD4 and CD8 lymphocytes: Induction of H1RBD and TCR stimulation binding TCR stimulation results in Glut-1 expression and concomitant glucose uptake in CD4 and ... and -2 SU amino terminus were generated by PCR and subcloned into the pCSI expression vector as fusion proteins harboring a carboxy terminal rFc tag [5], and are herein referred to as H1RBD and ... Jurkat and human erythrocytes were monitored by immunoblotting with an anti-C-term Glut-1 antibody TCR-induced expression of Glut-1 on both CD4+ and CD8+ T cells results in H1RBD/H2RBD binding and...
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Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf
Ngày tải lên :
14/08/2014, 16:21
... under physiological and diseased conditions Results and discussion Development and validation of the Human TReg Chip Whole-genome expression data from human and mouse CD4+CD25+ and CD4+CD25- T cells, ... confirmation of microarray results Real- time RT -PCR was performed for (a) TNFRSF1B, (b) TRAF1, and (c) LGALS3 expression in MACS isolated human CD4+CD25+ TReg and CD4+CD25- naïve T cells Fold changes ... confirmation of microarray results Real- time RT -PCR was performed for (a) FOXP3, (b) CTLA4, (c) CCR7, and RPS9 (data not shown) expression in MACS separated human CD4+CD25+ TReg and CD4+CD25- naïve T cells...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
Ngày tải lên :
14/08/2014, 19:22
... The expression of OVA and Fc expression could be detected in CHO cells by Western blotting, ELISA, and flow cytometry, as shown in Fig 1A and 1B The PCR products of OVA and Fc were selected as ... sensitized and challenged with OVA, OVA- pcDNA3.1-vaccinated mice sensitized and challenged with OVA, and OVA-Fc-pcDNA3.1-vaccinated mice sensitized and challenged with OVA * and ** stand for the ... sensitized and challenged with OVA, OVA- pcDNA3.1-vaccinated mice sensitized and challenged with OVA, and OVA-Fc-pcDNA3.1-vaccinated mice sensitized and challenged with OVA * and ** stand for the...
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Tài liệu Lab 4.2.3 Suspending and Disconnecting Telnet Sessions docx
Ngày tải lên :
11/12/2013, 14:15
... both the serial and the FastEthernet interfaces up? _ Step Suspend the current Telnet session a Enter Ctrl+Shift+6 followed by the x key This only suspends the session and returns to ... suspended Telnet session, type disconnect and press Enter Upon completion of the previous steps, logoff by typing exit Turn the router off 2-4 CCNA 2: Routers and Routing Basics v 3.0 - Lab 4.2.3 ... privileged EXEC mode, enter the command reload Router(config)#reload The responding line prompt will be: System configuration has been modified Save? [yes/no]: Type n and then press Enter The responding...
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