isolation identification and characterization of butanol tolerant bacteria

... dithiothreitol in a total volume of 50 lL Then, 40 lL of a reaction mixture composed of 200 lL of Hepes (1 M), pH 7.6, 40 lL of EDTA (0.2 M), 40 lL of NADPH (40 mgÆmL)1), and 500 lL of insulin (10 mgÆmL)1) ... with the addition of 10 lL of thioredoxin reductase, and incubation was continued for 20 at 37 °C The reaction was stopped by the addition of 0.5 mL of M guanidine-HCl and mM NBS2, and the absorbance ... ApTR (B) Molecular mass determination of ApTrx and ApTR Molecular masses of recombinant ApTrx and ApTR were determined by analysis of the elution files of standard proteins from a Sephacryl S-200...

Ngày tải lên: 23/03/2014, 21:20

... review, the utility of CODEHOP-mediated PCR for the identification of novel viruses and the characterization of new viral genes and genomic regions is presented While the focus of this study was ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function...

Ngày tải lên: 18/06/2014, 22:20

... et al.: Identification and characterization of a spontaneous ovarian carcinoma in Lewis rats Journal of Ovarian Research 2010 3:9 Page of Submit your next manuscript to BioMed Central and take ... Hiserodt JC: Development and characterization of a clinically useful animal model of epithelial ovarian cancer in the Fischer 344 rat American Journal of Obstetrics and Gynecology 1996, 175:593-599 ... asphyxiation and the cells within the peritoneal cavity harvested by flushing the abdomen with 35 milliliters of sterile phosphate buffered saline (PBS, Grand Island Biological Co., Gibco BRL, Grand Island,...

Ngày tải lên: 20/06/2014, 07:20

... mM EDTA, 1.5 M NaCl), in the presence of 0.1 mg of proteinase K and mg of lysozyme and incubated at 37°C for 30 Lysis was completed by adding ml of 20% SDS and incubated for h at 65°C with shaking ... species of bacteria including several unculturable bacteria in addition to the culturable proteolytic bacteria that are predominant and are involved in the degradation of the skin (Kayalvizhi and ... 65 and 85% of the maximum activity at the temperature range of 35°C and 55°C respectively Thermal stability of the purified AS-protease was estimated at Figure Multiple sequence alignment of...

Ngày tải lên: 21/06/2014, 05:20

... ‘chemical genetic’ approach of identifying and studying the mechanism of action of small-molecule agonists (and antagonists), we hoped to uncover some of the complexities of the Hh-signaling system ... activation with a Hh-protein ligand has therapeutic value [49,50] On the basis of our current understanding of these models and the specific mechanism of action of the Hh agonists, we predict ... binding of the Hh agonist to Smoexpressing cells This supports the model that all of these small-molecule modulators of Hh signaling are direct ligands of Smo We next asked whether a derivative of...

Ngày tải lên: 06/08/2014, 18:20

... experiments and participated in the design of the study and analysis of the data FC participated in the design of the study and in the analysis of data and helped to draft the manuscript PM cloned and ... the analysis and interpretation of data and helped to draft the manuscript RR participated in the design of the study and in the revision of the manuscript EP participated in analysis of data GV ... recombinant protein and helped to interpret the data CA participated in the design and revision of the study and performed the statistical analysis RP participated in the design and revision of the study...

Ngày tải lên: 09/08/2014, 08:22

... quantitatively significant product of FLSs [4] and little is known about the processing and function of the c19orf10 molecule Materials and methods Cells, tissues, and synovial fluid Synovial tissue ... rupole time -of- flight mass spectrometer [10], and proteins were identified by single MS (peptide mass fingerprinting) using ProFound [11] and by tandem mass spectrometry (MS/ MS) using Tandem search ... Expression of c19orf10 in OA synovium (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular...

Ngày tải lên: 09/08/2014, 10:20

... amino-acid sequence of CaNDR1a (Figure 4), with residues S189 and G190 being strong ω-site candidates (with P-values of 2.48 × 10 -6 and 2.76 × 10 -5 , respectively) Furthermore, CaNDR1a and its Arabidopsis ... constitute a novel report on the identification and characterization of a functional NDR1 homolog, despite the plethora of orthologous candidates In this study, several lines of evidence indeed demonstrated ... Maluf M, Fernandez D: Identification of coffee WRKY transcription factor genes and expression profiling in resistance responses to pathogens Tree Genet Genomes 2010, 6:767-781 19 Fernandez D, Santos...

Ngày tải lên: 11/08/2014, 11:21

... one of the most devastating diseases of wheat in China and worldwide and causing significant yield losses [28] High temperature, often combined with drought stress, causes yield loss and Page of ... proteinase K and DNA was extracted and dissolved in 50 μl of ddH2O Additional file 6: Categories of siRNAs corresponding to SRP1 and SRP3 7S RNA variants and sequences of SRP1 and SRP2 corresponding siRNAs ... Ct of the control products (Ta-actin) Author details State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and...

Ngày tải lên: 11/08/2014, 11:21

... Development of the flower shoot New Zealand Journal of Botany 1975, 13:473-483 Snelgar WP, Manson PJ: Determination of the time of flower evocation in kiwifruit vines New Zealand Journal of Crop and ... Page of 15 Figure Expression profiles of Actinidia flowering genes in normal and aberrant flowers Real-time RT-PCR analysis of the Actinidia flowering genes in the leaf and floral organs of A ... stages of the project, Andrew Gleave, Sakuntala Karunairetnam and Bhawana Nain Page 13 of 15 for cloning and sequencing support, Tim Holmes for photography, and Anne Gunson for critical reading of...

Ngày tải lên: 11/08/2014, 11:22

... Thr3, Thr11, and at several residues adjacent to them (Arg2, Lys4, Ser10, and Gly12) Using anti-H3T3ph antibodies, bands were detected in the case of normal histone H3 and S10A, T11A, and G12A mutants, ... Merged images of DNA (blue) and AtHaspin-tdTomato (red) are shown in color Scale bars: 10 μm aphidicolin (Figure 5A) This profile of AtHaspin expression was very similar to those of AtAURs and mitotic ... localization and dynamics of AtHaspin-FP reflect those of an endogenous Haspin protein in BY-2 cells This localization of AtHaspin-FP is consistent with that of phosphorylated H3T3 and H3T11 in...

Ngày tải lên: 11/08/2014, 11:22

... Page of 11 Figure Identification and characterization of pMD18-T/gC and pET32a-gC with restriction enzyme and PCR-based amplification (a) Identification of pMD18-T/gC with restriction enzyme and ... molecule, and the two glycoproteins, gC and gE, have a synergistic effect on mediating immune evasion[30,31] And gC of HSV-1 and -2, BHV-1, PRV, and Equine herpes virus types and (EHV-1 and -4) ... Liang X: Characterization of cell-binding properties of bovine herpesvirus glycoproteins B, C, and D: identification of a dual cell-binding function of gB J Virol 1995, 69:4758-4768 25 Moffat JF,...

Ngày tải lên: 12/08/2014, 02:20

... potential latency of WSSV and increases once the lytic stage starts This finding suggests that WSSV403 expression should contribute to the activation of lytic stage And such function of Page of (page ... protein extraction and Zhilong Wang for preparation of shrimp E2 enzyme References 10 11 12 13 Lightner DV: A handbook of shrimp pathology and diagnositic procedures for diseases of cultured penaeid ... latency-associated-genes, WSSV151, 366 and 427, were indicated as positive controls, while VP19 and VP28 were used as negative controls (C) Amplification profiles and dissociation curves of WSSV403 in real time...

Ngày tải lên: 12/08/2014, 04:21

... (+) if on the up strand, (-) if on the down strand or (*) if on both strands Number of occurrences is the number of occurrences of a motif and Number of promoters is the number of promoters containing ... Denote E( Ν w) and σ( Ν w) as the mean and standard deviation of ˆ Ν w The Z-score of w is defined as Zw ˆ ˆ = (Nw - E( Ν ))/σ( Ν w) It is nontrivial to compute the statistics of random variable ... optimization and w[j,k] represents the subsequence of w from its jth to kth positions, PWu is the transition probability of motif Wu, and and first j positions of Θu, respectively The computation of ˆ...

Ngày tải lên: 14/08/2014, 16:21

Ngày tải lên: 14/11/2014, 06:20

Ngày tải lên: 14/11/2014, 13:05

... activity of pooled fractions Chapter Three Figure 3.1: Purification of BF-AC1 and BF-AC2 from the venom of B fasciatus Figure 3.2: ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure 3.3: Structural characterization ... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Kang Tse Siang, Professor R Manjunatha Kini and Associate Professor Go Mei Lin for their constant encouragement and scientific input throughout my candidature Dr Kang and Prof Kini have provided...

Ngày tải lên: 09/09/2015, 11:08

... 41 Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; cDNA ... Regulation of Differentially Expressed Isoforms of Drysdalin Introduction 148 Materials and methods 153 Liver tissue; Kits and reagents used; Isolation of genomic DNA; Construction of genome walker ... walking; Isolation and sequencing of clones Results and discussion Chapter 155 Gene structure of a novel protein 513V5 Introduction 173 Materials and Methods 175 Materials; Genomic DNA isolation and...

Ngày tải lên: 11/09/2015, 10:02

... love, support and encouragement i Table of Contents Page Acknowledgements i Table of contents ii Summary vi List of Abbreviations ix List of Publications xv List of Tables xvi List of Figures xvii ... regulates the expression of SOC1 and FT 130 3.13 Spatial regulation of SOC1 and FT expression by J3 136 3.14 Expression of AP1 and LFY is regulated by J3 140 3.15 Induction of J3 expression immediately ... including LEAFY and three closely related MADS-box genes, AP1, CALIFLOWER (CAL), and FRUITFUL (FUL) (Ferrandiz et al., 2000; Ng and Yanofsky, 2001) Moreover, characterization of MADS-box genes...

Ngày tải lên: 11/09/2015, 10:02