isolation cultivation and application of human alveolar epithelial cells

... recruitment and activation of inflammatory cells in the alveolar space and the role of alveolar epithelial cells type II in the cytokine network of the lung, we investigated the effects of proinflammatory ... layer of alveolar epithelial cells type I and type II Type I cells function as a physical barrier, whereas type II cells produce surfactant and act as progenitors to replace injured alveolar epithelial ... increasing alveolar macrophage (AM) and particularly epithelial expression of CCL2 and CXCL8 as well as the expression of CXCL10 and CXCL11 is paralleled by pronounced recruitment of Mn, PMN, and Ly...

Ngày tải lên: 12/08/2014, 18:21

... the alveolar epithelium consists of a continuous layer of tissue made up of two principal cell types: flattened type I alveolar epithelial cells and cubodial type II alveolar epithelial cells ... aggravating tissue pathology Materials and methods Isolation of primary human alveolar epithelial cells Primary alveolar epithelial cells were isolated from human non-tumor lung tissue obtained ... restrictiveness of the alveolar cell preparation, the competence of the formation of tight junctional complexes within the polarized alveolar epithelial cells model can be assessed [16] and the cells were...

Ngày tải lên: 12/08/2014, 14:20

... stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells Respir Res 2006, 7:132 Publish with Bio Med Central and ... evidence of apoptosis in human lung cancer cells (H1299) Figure Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in human lung cancer cells (H1299) Human lung cancer cells ... evidence of apoptosis in primary human small airway epithelial cells Cigarette Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in primary human small airway epithelial...

Ngày tải lên: 12/08/2014, 15:21

... variety of alveolar epithelial cells and in primary human small airFigure way epithelial cells Cigarette smoke extract differentially caused cytotoxicity in a variety of alveolar epithelial cells and ... cells and in primary human small airway epithelial cells A Various alveolar epithelial cells such as human lung cancer cells (H1299), human adenocarcinoma cells (A549), human lung epithelial cell ... (H1299), human lung epithelial cells (H441), murine type II epithelial cells (MLE-15), and rat lung epithelial cells (L2) in addition to human adenocarcinoma cells (A549) Another aim of this study...

Ngày tải lên: 12/08/2014, 16:20

... ENaC and transepithelial Na + transport in primary human airway epithelial cells, and (ii) that EPIhNE4, a specific and potent inhibitor of hNE [22], could block this stimulation The objectives of ... therefore to test the effects of hNE and EPI-hNE4 on ENaC activity and transepithelial Na+ transport in vitro in primary cultures of human nasal epithelial cells from control and CF patients Experimental ... trypsin had no effect on sodium current in human nasal epithelial cells and in rat alveolar epithelial cells [15,21], suggesting that in these primary cells, ENaC was fully activated by epithelium-derived...

Ngày tải lên: 12/08/2014, 11:22

... was handled in a bio-safety level facility at the Department of Microbiology, The University of Hong Kong Isolation of primary human alveolar type II alveolar epithelial cells Primary type II alveolar ... Virus infection of type I-like alveolar epithelial cells Type I-like alveolar epithelial cells were infected with pdmH1N1 and seasonal H1N1 at a multiplicity of infection (MOI) of two Minimum ... H1N1 virus in human macrophages [7] As the tracheo-bronchial epithelium, type I and II alveolar epithelial cells and macrophages are key target cells for pdmH1N1 infection [8] and the most serious...

Ngày tải lên: 12/08/2014, 11:22

... F344 and Lewis rats Preparation of the dissected mammary gland complexes produced comparable amounts of epithelial cells in F344 and Lewis rats Marked differences between cells from F344 and Lewis ... decreased the number of cells only in P1-F344 -cells, but not in P2-F344- and P2-Lewis -cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not ... numbers of 266-691 cells were counted Statistical evaluation of data Data are mainly shown as change in number of cells (aamylase-treated) compared to control treated cells in percent (mean and standard...

Ngày tải lên: 10/08/2014, 10:21

... doi:10.1186/1743-422X-7-344 Cite this article as: Lam et al.: Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses Virology Journal ... role in the stimulation of B lymphocytes for antibody production TNF-a together with IL-6 may boost proliferation and differentiation of B cells, and proliferation of T cells As a result, all ... Kinetics of replication of different subtypes of influenza A virus in NCI-H292 cells NCI-H292 cells were infected with different influenza virus subtypes: H1 - H1N1/2002, H5 - H5N1/ 2004, and H9...

Ngày tải lên: 12/08/2014, 02:20

... Log10 (TCID50/0.1ml) Virus of and the expression of viral nucleoprotein (NP) and matrix protein (M1) in the infected cells was examined by using immunofluorescent staining and Western analysis, respectively ... induction of M1 gene (log10) (A) Figure Quantitative analysis of the RNA levels of M1 and PA in A549 cells (A) A549 cells were infected with H9N2/G1, H9N2/Y280 or H9N2/G9 at an m.o.i of or mock ... ##A/Chicken/HongKong/G9/97 humans [20,21] Furthermore, it has been demonstrated that H5N1/97 viruses replicate efficiently in primary alveolar epithelial cells and the viral titre of the infected cells is comparable...

Ngày tải lên: 12/08/2014, 04:20

... Petrofsky M, Goodman J: The efficiency of the translocation of Mycobacterium tuberculosis across a bilayer of epithelial and endothelial cells as a model of the alveolar wall is a consequence of ... molecules (P < 0.05 for CD86 positive cells and CD86 MFI; and P < 0.01 and P < 0.05 for CD40 positive cells and CD40 MFI respectively) Modulation of fresh human AECII phenotypic markers expression ... lack of expression of CD80 (B7-1) and a low expression of CD86 (B7-2) both on the A549 cell line and on the freshly isolated AECII [31] Only a slight increase in the densities of CD80 and CD86 and...

Ngày tải lên: 12/08/2014, 13:22

... pneumophila against human alveolar epithelial cells L pneumophila clearly induced the damage of alveolar epithelial cells in dose-dependent and time-dependent manners The administration of methyl prednisolone ... mean ± SD of three different experiments * P < 0.05 early stage of L pneumophila infection may decrease apoptosis in alveolar epithelial cells Clinical significance of alveolar epithelial cells infection ... intracellular growth and cytotoxicity of L pneumophila in A549 alveolar epithelial cells We also investigated the mechanisms of apoptosis of L pneumophila-infected A549 cells, including nuclear...

Ngày tải lên: 12/08/2014, 15:21

... cytoplasm of epithelial cells (A and D), and infiltrated lymphocytes (B and E) and macrophages (C and F) HTLV-I can be transmitted into lung epithelial cells from infected T cells and the integrated ... http://www.retrovirology.com/content/5/1/86 Figure Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 ... specimens of A549 and NCI-H292 cells at and days of culture contained no viable MT-2 cells As shown in Figure 1, A549 and NCIH292 cells cocultured with MT-2 cells showed strong expression of Tax...

Ngày tải lên: 13/08/2014, 05:21

... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...

Ngày tải lên: 13/08/2014, 05:22

... regulation of Treg and Th cells may contribute to the development of NP As the results of microorganism stimulation and inflammatory cells disorder, epithelial damage and remodeling occurred in NPs Epithelial ... by four types of cell: basal cells, goblet cells and ciliated or non-ciliated columnar cells Basal cells may involve into the epithelial repair after injury and other types of cells are more ... components and conditions for hNESPCs growth and differentiation in vitro Primary cells from biopsies survived and more than 90% of cells proved to have properties of epithelial stem or progenitor cells...

Ngày tải lên: 09/09/2015, 11:29

... indication of techniques for the treatment of this kind of surgical complication Spontaneous healing of to mm openings can occur, while untreated larger defects are connected with the pathogenesis of ... communications and those with a history of sinus disease, surgical closure is often indicated 1,2 Oroantral fistula (OAF) is an epithelialized communication between the oral cavity and the maxillary ... characteristics of OAC/OAF; the apexes of tooth 26 were in extremely close approximation to the maxillary sinus, and an area of periapical rarefaction was evident (Fig 1) After the failure of the endodontic...

Ngày tải lên: 25/10/2012, 11:48

... NATIONAL UNIVERSITY - HANOI COLLEGE OF FOREIGN LANGUAGES POST-GRADUATE DEPARTMENT DƯƠNG THỊ HỒNG AN AN EVALUATION AND APPLICATION OF SOME MULTIMEDIA PRESENTATION SOFTWARE IN DESIGNING ENGLISH LECTURES...

Ngày tải lên: 07/09/2013, 13:08

... 14 Review and Application of the Tulsa Liquid Jet Pump Model December 2006 15 Review and Application of the Tulsa Liquid Jet Pump Model December 2006 16 Review and Application of the Tulsa Liquid ... analysis of the data followed the model of Petrie, Wilson and Smart (PWS) This model is based on conservation of mass and energy, and is widely familiar to production engineers The PWS model and the ... of N and M that together result in the highest efficiency and lowest power demand for the power fluid As seen on Figure 11 and 12, increasing nozzle/throat area ratio Review and Application of...

Ngày tải lên: 23/10/2013, 08:42

... induce a range of cytokine expression in intestinal epithelial cells upon infection Comparison of the status of induction of cytokines by quantitative realtime RT-PCR in Int407 and T84 cells showed ... in infected epithelial cells A Bandyopadhaya et al Fig (A) Secretion of CT in tissue culture medium and in the presence of Int407 cells Different V cholerae strains (toxinogenic and nontoxinogenic) ... infected epithelial cells A Bandyopadhaya et al Fig Modulation of various cytokine mRNAs in Int407 cells by a ctxA mutant of V cholerae RT-PCR of the respective cytokines in Int407 cells (1),...

Ngày tải lên: 18/02/2014, 16:20

... demonstration of P2X7 in alveolar epithelial cells of wild-type (+ ⁄ +) and Caveolin-1-knockout (- ⁄ -) lungs Wildtype: Arrow indicates a negative alveolar epithelial type II cell and a neighboring ... alveolar epithelial type I cells [32], which cover more than 95% of the alveolar surface in lungs In our study, we tested four alveolar epithelial cell lines with characteristics of type I cells ... presence of the 80 kDa band alone Isolation of lipid rafts and localization of Caveolin-1 and P2X7 Insolubility in the detergent Triton X-100 is a wellrecognized biochemical characteristic of proteins...

Ngày tải lên: 19/02/2014, 00:20

... treatments of total N, P O and K 2O maintained the same level of 120-180120 levels Application of compost assumed 50% available N in composted hog and chicken manure and mushroom wastes, and 25% ... standards for factories and for the quality and prices of products, should be set by the government, and included officially in Taiwan’s Fertilizer Management Law Table Effect of compound and ... qualities of compost and chemical fertilizer, in a way which is cost effective and minimizes environmental pollution However, the quality control of organic compound fertilizer and of compost are of...

Ngày tải lên: 05/03/2014, 20:20