isolation and purification method of mouse fetal hepatoblasts
liver stem cells methods and protocols
... majority of the cells in the nonparenchymal cell fraction are single and round Bars indicate 100 m 8 N Shiojiri and M Nitou 3.2 Isolation of Fetal Mouse Hepatoblasts The separation of fetal mouse hepatoblasts ... medium used for the primary culture of mouse fetal hepatoblasts 4 Isolation and Purication Method of Mouse Fetal Hepatoblasts 37 water After dissolution of the insulin, the solution becomes ... hepatocytes Fig Schematic representation of the MFHs isolation/ purication method 4 Isolation and Purication Method of Mouse Fetal Hepatoblasts 39 and keep the fetal livers in HEPES solution on ice Prepare...
- 226
- 812
- 0
Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... lg of polyclonal anti-eEF1A (lane 4), with nuclear proteins and 0.9 lg of polyclonal anti-eEF1A (lane 5), with nuclear proteins and lg of total CRS (lane 6), with nuclear proteins and 0.9 lg of ... presence of lg poly(dIdC) and lg of CT as competitors, as described in the Materials and methods After 25 of incubation at room temperature, the samples were cross-linked by UV exposure and then ... lymphocytes were incubated with 1.5 lg of poly(dIdC), lg of CT and ng of c32P-labelled GT oligomer in a buffer containing 25% glycerol, as described in the Materials and methods After incubation at room...
- 12
- 552
- 0
The Fraction of Cancer Attributable to Lifestyle and Environmental Factors in the UK in 2010 docx
... cases of cancer in the UK (approximately 134 000 new cases in 2010), and about 50% of all cancer deaths Most of these cases of cancer (excluding a few thousand due to the natural background of ionising ... trained in many branches of medicine and scientists from almost all of the biological disciplines and beyond British Journal of Cancer (BJC) exists to serve the needs of this diverse community, ... the attention of researchers, individuals and policy makers on the relative importance of the currently known causes of cancer Conflict of interest The author declares no conflict of interest This...
- 92
- 563
- 0
d. of c 219. You should get into the habit of ……………… at least one newspaper daily. a. pot
... understanding b To understand c Understanding d For understanding c 396 If England had not imposed a tax on tea two hundred and twenty years ago, …………… the United States have remained part of the British ... getting a good job a much b many c both a and b are correct d little b 389 …………… our products are sold overseas a Many of b Much of c A great deal of d Little of a 390 …………… screws have come loose ... the deaf and the blind a much b a little c many d little c 395 …………… electricity depends on a knowledge of atoms and the subatomic particles of which they are composed a The understanding b...
- 28
- 416
- 0
Báo cáo toán học: "The Fraction of Subspaces of GF(q)n with a Specified Number of Minimal Weight Vectors is Asymptotically Poisson" pot
... distribution of weight w points is asymptotically the k −1 same among random sets of qq−1 points and among random (k − 1)-dimensional projective subspaces, namely Poisson with parameter λ Proof of the ... bijection between sets of w minimally dependent vectors among the columns of H and (q − 1)-tuples of vectors of weight w in V , where a tuple consists of all nonzero multiples of a weight w vector ... contain at most j(q − 1) vectors of weight w and no nonzero vectors of less weight Since this is a downset in the poset of subspaces of GF(q)n , the fraction of k-dimensional subspaces that lie...
- 8
- 421
- 0
Báo cáo khoa học: "Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum" pot
... by BCL buffer A total of × 106 cells were incubated with each of FITC-conjugated rat anti -mouse CD3, mouse anti -mouse Ly-49AB60 (PharMingen, BD Biosciences), mouse anti -mouse MHC class II I-Ab/I-Ad ... NIH/3T3, SNU1, and SNUC2A, and of 104 cells per well for Jurkat cells in 96-well plates Six hrs later, serial dilution of cytoplasmic fraction of LABs from 250 µg/ml was added to wells, and incubated ... administration of cytoplasmic fraction of B longum (B) and L casei (L) at 100 mg/kg/day, and Y400 (Y) at 2.6 ml/kg/day as a dietary supplement b Increased survival rate of tumor-bearing mice by cytoplasm of...
- 8
- 645
- 0
Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
... design CB, JC and JPP participated in the acquisition of data CB, JMP, JC and JPP participated in the analysis and interpretation of data CB, JM-P and JPP prepared the manuscript CB, JM-P and JC participated ... chondrocytes [4] They can also inhibit the stimulating action of IL-1β on stromelysin and collagenase and inhibit production of IL-6, IL-8 and prostaglandin E2 [5] In addition, it was demonstrated that ... superficial zone of cartilage corresponds to the superficial and the upper intermediate layers, and the deep zone to the lower intermediate and deep layers The total number of chondrocytes and those...
- 9
- 547
- 0
Effect of size and volume fraction of reinforcement on the properties of light metals
... advantages of ease of processing a wide variety of matrix metals, and the control of orientation and volume fraction of the fibers The main problems associated with this process are fiber degradation and ... material and avoids the formation of overspray powders It offers both the features of finer grain size and low segregation of 11 Literature Research reinforcement of spray processes, and the simplicity ... matrix and therefore possess high interfacial strength [11] The main disadvantages of this method are the high viscosity of melts, limitation in choice of reinforcement-matrix systems, and difficulty...
- 168
- 751
- 0
On the hilbert uniformization of moduli spaces of flat g bundles over riemann surfaces
... can be stated as follows The upper side of Ri,j is glued to the lower side of Ri,σi (j) and the left hand side of Ri,j is glued to the right hand side of Ri+1,j Arbitrary choices won’t induce ... uniformization is as follows The upper side of Ri,j is glued to γ ξ ξ i,j the lower side of Ri,σ and the left hand side of Ri,j is glued to the right i (j) ξ hand side of Ri+1,j Again we need to impose ... Hr [K] the Hurwitz space of K-sheeted coverings with r ≥ branch points 20 (1) The number b0 (M1,1 [K]) is a function of the number of partitions of K and the number of all transitive subgroups...
- 207
- 189
- 0
EVALUATING THE EFFECT OFWEATHER CONDITIONS ON THE SOLAR FRACTION OF SOLAR ASSISTED HEATING SYSTEM ĐÁNH GIÁ ẢNH HƯỞNG của điều KIỆN THỜI TIẾT đến hệ số NĂNG LƯỢNG mặt TRỜI của hệ THỐNG NHIỆT NĂNG LƯỢNG mặt TRỜI
... highest values of the electric energy demand of pumps for draw-offs are in the early afternoon while the differences of the electric energy demand of pumps for morning and noon profiles differ ... heating supply (consist of useful heat gain of solar collectors and auxiliary heat of boiler) and the total heat demand (consist of domestic hot water and space heating demand) is 5.87(kWh) This ... heating supply (consist of useful heat gain of solar collector and auxiliary heat of boiler) and the total heat demand (consist of domestic hot water and space heating demand) is 11.8(kWh) This...
- 6
- 383
- 1
Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"
... relatively low expression of Cx43 and Cx40 in VSMCs and endothelial cells was observed in the normal group However, the expression of Cx43 and Cx40 in the intima and tunica media of the high fat diet ... expression of Cx40 (panel A) and Cx43 (panel B) in the rabbit iliac arteries of different groups by RT-PCR Top panels represented mRNA expression of Cx40 and Cx43 from each of the groups and bottom ... mRNA expression of Cx43 and Cx40 by RT-PCR As shown in Figure 5, after 2% agarose gel electrophoresis, the bands were visualized and length of amplified products of Cx43, Cx40 and GAPDH was about...
- 8
- 467
- 0
Báo cáo y học: "Mechanical complications and reconstruction strategies at the site of hip spacer implantation"
... reconstructive techniques for management of extensive loss of femoral and/ or acetabular bone Mechanical complications and reconstruction strategies The exact rate of mechanical complications following ... reimplantation, and that during removal of the prosthesis stem osseous defects might occur Recently, the “glove”-technique has been described as new method for femoral fixation of hip spacers [3] This method ... This implantation technique also offers the advantage of a prevention of a spacer migration into the pelvis (Figure 4) beside a normal articulation and prevention of any spacer dislocation Hereby,...
- 6
- 455
- 0
International payment service by opening Letter of Credit at Vietnam Eximbank Hanoi Branch.doc
... increase both in term of revenue and in the amount of items And the settlement of Branch in that year also had much increased in the number of items and its value The turnover of 92, 533.57 USD in ... proficient in documentary credit business, UCP500, and relevant laws; their technical and professional level of document checking up and dispute handling are the first class in banking sector of ... domestic and international significant events, especially become the official member of WTO The amount of trading with foreign companies has got a sharp increase, and the volume of imported and exported...
- 39
- 2,013
- 30
Precluding and reducing solutions to credit risk at Quang Trung branch of Vietnam Bank of Investment and Development.doc
... Investment and Development of Vietnam – the first unit of the system of Bank for Investment and Development of Vietnam to deploy the Project of Modernization of the payment system and the modernization ... technology and business norms of a modern bank However, according to the present regulation of the State bank and the Charter of Organization and Operation of Bank for Investment and Development of Vietnam, ... expand the operational network of Bank for Investment and Development of Vietnam in Hanoi City, assisting Bank for Investment and Development of Vietnam to seize and take the opportunity to expand...
- 47
- 892
- 14
Designing & evaluating an English reading test for the non-majors of Civil Engineering at Haiphong private university
... terms of method, there are paper -and- pencil language tests, performance tests, ect And in terms of purpose, there are achievement tests, proficiency test, and so on In fact, the same form of test ... obtain different profiles of a learner’s performance in the language The development and the use of language tests involve an understandingof the nature of communicative language use and language ability, ... 5.Design of the study The study is composed of three parts: *Part I is the presentation of basic information such as the rationale, the scope of the study, the aims of the study, the methods of the...
- 51
- 1,150
- 7
Teaching speaking skill for non-major ma students of english at vnuh
... several sets of pictures, depending on the size of the class 34 Have students use a clean sheet of paper and write Topic at the top of the left-hand side, and Comment at the top of the right-hand side ... analysis of the current situation of teaching and learning speaking skill here based on the result of interviews and surveys of student’s attitude towards the current teaching methods and activities ... indebted to my husband and my family for their understanding, patience and support during the entire period of my study Nguyễn Thị Thu Hương ii LIST OF ABBREVIATIONS AOL Age Of Learning C Consonant...
- 59
- 1,001
- 1
Techniques for improving listening skills FOR gifted students at Foreign Language Specializing School - College of Foreign Languages - VIETNAM National University, HANOI
... to the stress and intonation patterns of English and the way these influence both the realization of certain phonemes and the meaning of the utterance The number of homophones and homonyms in ... speakers of English and they have time to live , work and study in a native English environment Most of the teachers of FLSS are well aware of the important role of the ability to listen to English and ... students of English of FLSS, they have to deal with a lot of difficulties in learning to listen to English However, the majority of the students have strong and clear motivation and as a result of...
- 47
- 1,633
- 18
Action research on students’ inactiveness in pre-writing group discussion of an essay-writing essay practice lesson at hong duc university,thanh hoa
... the method of study, the scope of the study, the subject of the study, the significance of the study and the design of the study Part 2: Development consists of Section A ‘ Literature Review’ and ... Cause -and- Effect Analysis Essay and Argumentative Essay Among those, a Cause -and- Effect essay was not mentioned by Fawcett and Sandberg In Cause -and- Effect essay the writer identified the causes of ... terms of their academic course because in Term and Term of the Course students are at the levels of intermediate and advanced of English Teaching writing essays in class in the early stages often...
- 45
- 855
- 1
Designing an esp syllabus for the second-year students of library study at the national teachers training college
... List of figures and tables PART I: INTRODUCTION . 1 Rationale .1 Aims and Objectives . Research questions .2 Scope of the Study. .2 The significance of the study. .2 Methods of ... determine the content of the syllabus, the materials and the method of teaching/ learning purposes 1.1.2 Types of ESP There are many types of ESP, but the major distinction is often drawn between ... audio-lingual method was used in the 1950s and 1960s,however, this method received a lot of criticisms because of its theory and techniques 1.2.3.9 Analytic syllabuses This kind of syllabus see...
- 76
- 737
- 4
The application of games in teaching grammar with reference to tieng anh 10 textbook at ha trung high school, thanh hoa province
... games and instead of moans and whining, the teacher and the students get smiles and excitement 1.3.1 Benefits of teaching grammar with games Arif Saricoban and Esen Metin (2000), authors of "Songs, ... can’t understand immediately and 1.2% can’t understand This means that the effectiveness and ability of students’ lesson understanding is low 14 1.2% understand 43.8% 55% not understand immediately ... likely to stand the risk of fossilization or reach a point where they can cope with level of communication that is demanded of them by making use of their existing grammatical resources and communication...
- 39
- 1,577
- 8
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25