isolation and growth of adipose tissue derived stem cells

Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx

Ngày tải lên : 10/08/2014, 23:21

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... as: Pak: Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose- tissue- derived stem cells: a case series Journal of Medical Case Reports ... multiple growth factors including TGFb, IGF, FGF, and PDGF A literature review of the data on the uses of PRP showed that it has a positive effect on the stimulation of bones and blood vessels and...

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Báo cáo khoa học: Mechanisms of obesity and related pathologies: The macro- and microcirculation of adipose tissue pdf

Ngày tải lên : 29/03/2014, 22:21

... tumor cells, adipocytes and the adipose microvascualture, and the marked similarities in tumor growth and hypoxia compared with those of adipose tissue expansion remain of great interest Adipose tissue ... quality of adipose tissue consistently point to the microvasculature and angiogenesis within adipose tissue as critical role players in adipose tissue health and expansion [3] Expansion of adult adipose ... modu- Adipose tissue circulation lator of adipose depot physiologies and their systemic impacts [6,10,11] Adipose tissue vasculature Adipose tissue possesses a relatively dense network of blood...

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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps

Ngày tải lên : 07/08/2014, 23:22

... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have ... Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury Spinal Cord 2007, 45, 15-24 12 Hipp J, Atala A Sources of stem cells for ... analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue Stem Cells 2006, 24, 1294-1301 21 Kim JW, Kim SY, Park SY, Kim YM, Kim JM, Lee MH, Isolation and characterization...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... immunomodulatory effects of canine adipose tissue- derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS Improvement of neurological deficits ... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells...

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Báo cáo khoa học: Mechanisms of obesity and related pathologies: Transcriptional control of adipose tissue development pdf

Ngày tải lên : 29/03/2014, 22:21

... muscle actin and platelet -derived growth factor b These progenitors only existed in the vasculature of adipose tissue, and not in other organs, demonstrating that this population of mural cells is ... the white and brown depots Adipose tissue is the most abundant tissue in humans, representing approximately 10–29% of body weight It is found in a multitude of locations and consists of two major ... forms: white adipose tissue (WAT) and brown adipose tissue (BAT) WAT is the main site for the storage of energy in the form of triglycerides located in large lipid droplets that occupy most of intracellular...

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Báo cáo khoa học: Benzo[a]pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for a common food pollutant doc

Ngày tải lên : 30/03/2014, 11:20

... accumulation of toxins in adipose tissue was recently demonstrated by the observed increase in plasma levels of organochlorines released from adipose tissue during weight loss [7–9] The amount of pollutant ... concentrations of a common food pollutant, B[a]P, caused a rapid, direct and profound inhibition of adipose tissue lipolysis stimulated by epinephrine, dobutamine, salbutamol, BRL37344 and ACTH This ... Maurice and Erwan Magueur is greatly appreciated This work was supported by grants from the Lorraine Region and Urban Community of Grand Nancy (CUGN) and from the French Ministry of Research and...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... stem cells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells ... characterisation of human adipose tissue- derived stromal cells J Cell Physiol 2001, 189:54-63 Chiou M, Xu Y, Longaker MT: Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adiposederived...

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Báo cáo y học: " Histology of adipose tissue inflammation in Dercum’s disease, obesity and normal weight controls: a case control study" doc

Ngày tải lên : 11/08/2014, 03:20

... higher levels of interleukin (IL)-13 and levels of fractalkine were detected In previous reports and studies, an inconsistent picture of the histological appearance of the adipose tissue in DD ... High-power image of Figure Adipose tissue from the knee Haematoxylin-eosin staining One infiltrate of this size and only a few additional inflammatory cells gave a score of I Two infiltrates of this size ... inflammation of the nerves in the adipose tissue of the painful sites [1,20], which has only been confirmed in one case report [21] As regards inflammatory signs in the adipose tissue in DD, leukocytes and...

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Histology of adipose tissue inflammation in Dercum’s disease, obesity and normal weight controls: a case control study pdf

Ngày tải lên : 11/08/2014, 06:23

... higher levels of interleukin (IL)-13 and levels of fractalkine were detected In previous reports and studies, an inconsistent picture of the histological appearance of the adipose tissue in DD ... High-power image of Figure Adipose tissue from the knee Haematoxylin-eosin staining One infiltrate of this size and only a few additional inflammatory cells gave a score of I Two infiltrates of this size ... inflammation of the nerves in the adipose tissue of the painful sites [1,20], which has only been confirmed in one case report [21] As regards inflammatory signs in the adipose tissue in DD, leukocytes and...

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Characterization of human adipose tissue derived adult multipotent precursor cells

Ngày tải lên : 12/09/2015, 09:55

... use of stem cells in regeneration of bone 1-1 1-1 1-2 Chapter – Literature Review 2.1 Description of the adipose tissue 2.2 Adipose tissue – a possible alternative to bone marrow as a source of ... 3-3 3-3 3-5 Chapter – General Materials and Methods Chapter – Viability and adipogenic potential of human adipose tissue derived stem cells isolated from tissue aspirated with a vacuum pump or ... supportive of MSC growth 2– Chapter Literature Review The most important features of adipose tissue as a cell source might be the easy expendability of its MSC and the consequent ease of procurement of...

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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells

Ngày tải lên : 12/09/2015, 21:26

... during injury, stem cells can adopt this form of cell division to expand and replenish the depleted stem cell pool (Morrison and Kimble, 2006) 1.4 1.4.1 Types of stem cells Embryonic stem cells During ... 1.3 Symmetrical and asymmetrical division 1.4 Types of stem cells 1.4.1 Embryonic stem cells 1.4.2 Somatic stem cells 1.4.3 Stem cells and cancer ... maintain these cells in an undifferentiated, quiescent state For stem cells to be activated, the niche environment must change (Fuchs, 2007) 1.4.3 Stem cells and cancer Stem cells and cancer cells share...

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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

Ngày tải lên : 14/09/2015, 13:38

... potency of stem cells 1.1.2 Fundermental features of stem cells 1.1.3 Signalling pathways modulating pluripotency in stem cells 1.1.4 Transcription factors controlling pluripotency of stem cells ... Developmental potency of stem cells Stem cells are unspecialized cells that have the capability of self-renewal for producing identical unspecialized daughter cells and the potential of differentiation ... specialized cells Stemness refers to the undifferentiated status of self-renewing stem cells Stem cells show a hierarchy of developmental potency in mouse (Fig 1-1) Totipotent stem cells include...

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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên : 05/09/2013, 09:08

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Ngày tải lên : 18/02/2014, 16:20

... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution proﬁle of P III and P IV Chromatofocusing chromatography...

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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Ngày tải lên : 21/02/2014, 00:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... [19] Cells were harvested when growth reached the stationary phase and were stored frozen ()80 °C) Preparation of crude extract Cells were washed twice in 50 mM Hepes/NaOH buffer, pH 7.0, and...

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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Ngày tải lên : 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), conﬁrming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... the ﬁrst time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The reassessment of previous spectroscopic...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Ngày tải lên : 08/03/2014, 16:20

... Chlamydomonas cells Chlamydomonas CW15 cells were grown to a cell density > ´ 106 cells per mL Cells were pelleted, resuspended in 30 mM Tris/HCl pH 7.9, and broken by two cycles of freeze-thawing ... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF ... BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, except BAS1 of barley,...

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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Ngày tải lên : 16/03/2014, 18:20

... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... purity of mitochondria prepared from green tissue of tobacco, pea and Arabidopsis thaliana Phyton 43, 215–229 53 Leegood RC & Malkin R (1986) Isolation of sub-cellular photosynthetic systems In ... nutritional value of plant species short of S-containing amino acids By producing transgenic d-CDes plants this goal might be reached Experimental procedures Growth and harvest of plants Seeds of Arabidopsis...

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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

Ngày tải lên : 17/03/2014, 10:20

... respectively, and the resultant VNARs evaluated for retention of binding afﬁnity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... ampicillin and/ or 2% (w/v) glucose Solid media contained 2% (w/v) Bacto-agar Transformation of E coli was by standard procedures [24] performed using electrocompetent cells Isolation of total RNA ... possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details of the library construction are given in greater detail in the Materials and...

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