isolation and expansion of mscs from various sources

... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... area, and their ratio, as index of complexity Statistical analysis Data were expressed as mean ± standard deviation (SD) or median and range A comparison of the morphometric data obtained from ... involvement of FBs in CD are actually obstructed by the absence of a specific models; we therefore aimed this study to isolate and culture primary FBs from endoscopic duodenal biopsies of CD and non-CD...

Ngày tải lên: 18/06/2014, 15:20

... Discussion and conclusions Chapter Isolation and characterization of components from ethyl acetate fraction of Ixeris sonchifolia Hance 48 55 3.1 Introduction 56 3.2 Materials and methods 56 3.2.1 Isolation ... flavonoids, isoflavonoids and neoflavonoids 76 3.4.3 Skeletal structures of subgroups of flavonoids 76 HMBC and 66 NOESY XI 4.3.1.1 Effects of flavonoid compounds to on the viability of H2O2exposed ... observe, feel and understand the world I’m writing this thesis to share with all of them I Table of contents Acknowledgements I Table of contents II Abstract VII List of tables X List of figures...

Ngày tải lên: 11/09/2015, 10:06

... proteins In view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvularia allergens, and of the allergies ... extracts had various drawbacks such as chances of contamination from other allergen sources being prone to proteolysis, degradation and at times containing various lipopolysaccharides and endotoxins ... found to be the fungus of great importance locally, and a better understanding of allergens from this genus would assist in better understanding the allergic reactions and would also aid towards...

Ngày tải lên: 14/09/2015, 08:47

... E-value of 10-3 was used as an optimal cut-off This cut-off was previously standardized in-house by comparing the percentage of non-significant identity with various E-values as possible cut-offs ... components from species of Cladosporium (Cladosporium herbarum and Cladosporium cladosporioides) and from Penicillium notatum were also blotted and developed The western blots showed that each of the ... identifications Hence, bands between the molecular weight range of 15-50kDa were sent for mass spectrometric analysis A total of bands were cut (Figure 2.6) Out of the bands cut, bands corresponded...

Ngày tải lên: 14/09/2015, 09:09

... proofreading activity A 50µl reaction consisted of 0.5µl of each primer (100µM), 5µl of Expand Long Template Buffer 1, 2µl of dNTP (10µM), 3µl of cDNA and 1µl of Expand Long Template Enzyme mix containing ... further understand and establish the allergenicity of the various putative allergens of Curvularia lunata, immunological characterization of the putative allergens was performed The first and foremost ... colonies were then picked and suspended in 6µl of sterile water, out of which 1µl was used as the template in the PCR A ten-reaction master mix consisted of 10µl of 10X Buffer, 2µl of primers specific...

Ngày tải lên: 14/09/2015, 09:10

... other SODs from human and fungi suggesting that the levels of SODs in the indoor 149 Figure 4.5: 3D models of various MnSODs Monomeric and tetrameric (top view) subunit of the SOD is taken from Sabine ... dust as well A total of 12 samples from bedroom floors, samples from bedroom carpets, 13 samples from living room floors, 17 samples from living room carpets and samples from kitchen floors were ... 4.2 MATERIALS AND METHODS 4.2.1 Cloning, expression and purification of the C lunata allergens homologs from various fungal as well as non-fungal sources 4.2.1.1 Fungal cultures and raw materials...

Ngày tải lên: 14/09/2015, 09:15

... and critical residues for allergenicity, levels in the environment, function, etc Here, we report various allergens from Curvularia lunata For the rapid isolation of the allergenic proteins from ... faster and high throughput way of identifying allergens based on sequential similarity A total of 1683 ESTs were sequences and catalogued from a nonnormalized C.lunata library More than 50% of the ... extracts The problem with the use of crude allergen extract is that it’s a heterogeneous mix of proteins and has varying amounts of antigens The level of allergens may vary from batch to batch Also,...

Ngày tải lên: 14/09/2015, 09:15

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mistletoe lectin I [viscumin] from Viscum album [7], modeccin [8] and volkensin [9] from the roots of Adenia digitata and Adenia volkensii, respectively, and pulchellin from Abrus pulchellus seeds...

Ngày tải lên: 18/02/2014, 16:20

... (from residue number 3) to the sequence of the corresponding component of PH from A calcoaceticus NCIB 8250 (11/11 identity) [3] and very similar to that of the corresponding component of PH from ... samples Spectra were recorded from 260 to 190 nm at a speed of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of...

Ngày tải lên: 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... corresponds to 14% of the total iron absorption, and each NrfH heme corresponds to 7% From previous EPR studies [23], two sets of low-spin ferric g-values (gmax at 2.96 and 3.20) and a highspin heme...

Ngày tải lên: 21/02/2014, 00:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

Ngày tải lên: 07/03/2014, 21:20

... previously [21] Chloroplastic thioredoxins m and f from spinach were a kind gift of P Schurmann (University È of Neuchatel, Switzerland) Thioredoxins m and h from à Chlamydomonas were puri®ed as described ... ef®ciencies of two Trx isoforms f and m from spinach, because no Trx f from Chlamydomonas has been isolated until now Both were active with Ch-Prx1, but while Trx f was as ef®cient as Trx h and m from ... NcoI and BamHI, and cloned into the pET-8c vector Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas was a gift from  Á M Goldschmidt-Clermont (Universite de Geneve, Switzerland)...

Ngày tải lên: 08/03/2014, 16:20

... of several d-cysteine derivatives, and also the formation of d-cysteine or d-cysteine-related amino acids from b-chloro-d-alanine in the presence of various thiols or from O-acetyl-d-serine and ... counting from the start methionine was predicted, indicating a presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and ... d-cysteine Balance studies showed that 1.58 lmol of pyruvate, 1.63 lmol of NH3, and 1.47 lmol of elemental selenium were produced from 0.75 lmol of d-selenocystine When the reaction was carried...

Ngày tải lên: 16/03/2014, 18:20

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

Ngày tải lên: 07/08/2014, 18:21

... logarithm of the highest dilution of serum that could neutralize 100 TCID50 of the virus Total RNA was extracted from the giant pandas’ spleens, infected and non-infected FCWF cells (as positive and ... cytoblast of an infected FCWF cell The black arrow indicates the inclusion bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus strain from ... References Fig Analysis of GPV based on gene sequencing (A) Comparison of amino acid sequences of S gene product from GPV by nested PCR assay with that of the S gene of canine coronavirus (CCV)...

Ngày tải lên: 07/08/2014, 23:22

... we report the isolation of HPAI H5N1 from donkeys living in contact with diseased birds and demonstrate the presence of H5 seropositive ones in the neighbouring areas Methods Virus isolation Nasal ... letters showed site of H274Y and N294S substitutions (H275Y and N295S in N1 influenza numbering) cough, fever and serous nasal discharge The course of the disease was short (72 h) and responsive well ... concern of different issues including the route of transmission to donkeys, whether being from aerosol exposure of pulverized infected birds droppings or contaminated feeds and water or because of...

Ngày tải lên: 10/08/2014, 05:21

... encoded HTMT from R sativus HT performed the isolation and heterologous expression of the HTMT encoding gene from R sativus and characterization of the enzymatic properties of recom- Page of 10 (page ... purposes) BMC Plant Biology 2009, 9:116 P P P Emission profiles and rates of CH3I from T aestivum, O sativa and R sativus The emission profiles of CH3I from three plant species were measured (Figure 1) ... of CH3I, and that CH3I emission is affected by iodide concentration In vivo production of CH3I was observed from T aestivum, O sativa and R sativus when I- Figure 1profiles of methyl iodide from...

Ngày tải lên: 12/08/2014, 03:21

... Bezanilla JC, Toth TE, Meng XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in the respective ... DNAstar software package Sequence analysis based on the complete ORF2 sequence of the isolate in this study and other 10 genotype HEV isolates in China, using DNAstar software package Page of (page ... participated in the planning of the project XGH was the leader of the project WZ, SXY, QS, and TLS collected all samples and performed the RT-PCR experiments for HEV RNA WZ and SXY did the phylogenetic...

Ngày tải lên: 12/08/2014, 04:21

... Identification of MPs by flow cytometry Representative dot plots and histograms of MPs from sputum from CF patients and P aeruginosa and S aureus MPs (A), P aeruginosa PAO1 (B), S aureus (C) and calibrator ... identification and characterization of MPs in the sputa of CF patients Patients and Methods Study patients The study was approved by, and performed in accordance with, the ethical standards of our institutional ... microscopy of MPs from the sputum of a CF patient Multiple spherical particles, ranging from 100 to 500 nm, are visualized Page of * A) 7.5 50 25 stable PCD CF B) Figure Quantification of MPs in CF and...

Ngày tải lên: 12/08/2014, 11:22

... products from a Primer RAPD reaction are shown in Figure Reactions with isolate V1 and T 551 gave similar bands but some of the T 551 bands were weaker The patterns of isolates T 603 and T 657 ... isolates from DD described to date originate from the USA (Iowa and California), the UK and Germany [8,11,14-16] They represent three yet unnamed phylotypes and T brennaborense and two thirds of the ... weak bands and a smear at the bottom of the gel Gel plugs with different cell densities from three different cultures and occasions were made for isolate T 657 without obtaining a distinct banding...

Ngày tải lên: 12/08/2014, 18:22