ischemia reperfusion induceddamage through activation of the cholinergic anti inflammatorypathway
Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"
... addition, there was a trend toward increased frequency of activation of the MET service during these hours in the years following the introduction of the MET system When directly compared with the ... 42% of the day), a substantial proportion of MET calls occur after normal working hours (53% of calls during 58% of the day), with the peak time of activity occurring between 20:00 and 20:30 These ... was then constructed from the 2568 episodes of MET service review to illustrate the frequency of activation at various times over the 24-hour period Episodes of activation were related to the...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf
... step in the activation of mitochondria-dependent apoptosis [12], the assembly of the apoptosome Upon formation of this complex, caspase-9 acquires the ability to trigger the processing and activation ... the activity of caspase-3 in the three cell lines Effect of HSS knockdown on CCCP-induced apoptosis To further elucidate the functional role of HSS in CCCP-induced apoptosis, the expression of ... from the generation of an electrochemical gradient across the IM forms the basis of the IM wm During cell death, MP often increases, allowing for the release of soluble proteins The only mechanism...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... play an important role in the formation of the MT1-MMP–furin complex; therefore, 3168 disruption of the interaction between GRASP55 and furin should perturb the activation of pro-MT1-MMP and reduce ... important for the interaction of MT1-MMP ICD with the PDZ2 domain of GRASP55 The interaction of PDZ domains with ICD internal motifs has previously been reported For example, the interaction of the ErbB2 ... the previously observed intracellular activation of pro-MMP2 [59] Mutation of the LLY573 motif to AAA573 in the MT1-MMP ICD has been reported to significantly decrease the internalization of the...
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Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
... with such a mechanism The most parsimonious explanation of the loss of the potentiating effect in the mutant receptor is that the mutation results in the loss of the ability of dTC to act as such ... Representation of the extracellular domains of the c–a subunits based on the crystal structure of AChBP The positions of cE93 and aR55 at the subunit interface are indicated and modeling suggests that these ... the loop D domain) as specific sites of ligand incorporation [5,15-17] In the present study, we have investigated the effects of mutations of the equivalent residue (aR55) lying in loop D of the...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
... Alternatively the cells were left in the presence of the antibody for the complete length of this period In both cases the result was the same As shown in Fig 7, the ligation of the CD53 expressing ... determine if the activation of JNK by ligation of the human CD53 antigen was a common signal response shared with other cells of the lymphoid lineage, we used the Jurkat Luciferase assays of transcriptional ... (B) the quantification was determined by the ratio of the signal of the radioactivity in the GST–Jun and GST–ATF2 fusion proteins with respect to the signal for the HA epitope As reference for the...
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Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc
... can be excluded Taken together, the effects of NGF on the Smad pathway are independent of TGF-b ligand To characterize the point of convergence and the mode of Smad activation, C-terminal phosphorylation, ... and the cells were analysed by confocal microscopy The projection of multiple sections is seen on the left for each panel to visualize the morphology of the cells The middle row shows staining of ... Details of polyclonal antisera against Smad2 (anti- S2), Smad3 (anti- S3) and C-terminally phosphorylated forms of Smad1 (anti- PS1) and Smad2 (anti- PS2) were published previously [31,32] The P-Smad1 antibody...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... activity (Fig 1B) These rapid effects of d-glucose suggest action at the membrane level, possibly during the transport of glucose into the cell We therefore studied the effect of 2-deoxy-d-glucose, ... when used at 10 mm The blocking of the ATPase activating effect of glucose was not the result of a toxic effect of 2-deoxy-d-glucose, as subsequent incubation in the presence of 10 mm glucose resulted ... and the findings described below, suggest that the decrease of HAT in the plasma membrane should be interpreted as a lower level of the H+-ATPase–tubulin complex We therefore presume that the...
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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx
... could lead to substantial activation of the reporter even in the complete absence of IRF-3 Nevertheless, maximal activation of the IFN-b promoter depended on the presence of both IRF-3 and IRF-7 ... role in the context of a given promoter if the arrangement of cis-acting elements allows them to occur Synergistic activation of the IFN-b gene promoter in insect cells The ability of the IFN-b ... families and the lack of viability resulting from gene targeting of either p300 or CBP places restrictions on the use of mammalian cells to dissect the activation mechanism of the IFN-b gene The IFN...
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Báo cáo khoa học: Upstream and intronic regulatory sequences interact in the activation of the glutamine synthetase promoter pot
... sequences of the upstream region and of the rst 1938 nucleotides of the rst intron of the rat GS gene were reported [5,6] This intron sequence ends at the EcoRI restriction site (Fig 1) The remaining ... In the case of the C2/7 cells, the concentration of foetal bovine serum was reduced to 1% to induce the formation of myotubes Harvest of the cells was carried out 48 h after transfection in the ... on the promoter Similar to liver cells, the stimulatory effect of either element was largely lost if the other element was absent Furthermore, and again similar to liver cells, the effects of the...
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Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx
... of K31, together with analysis of PKCe knockout MEFs with knockdown of PKCd, revealed further evidence of a crucial role of K63-ubiquitination of the RING domain of TRAF2 for recruitment of the ... understanding of the molecular mechanisms involved in TNFR1induced activation of the IKK complex and the classical NF-jB pathway might thus become possible only with the help of mathematical modeling The ... of a detailed and quantitative model of TNFR1induced NF-jB activation, however, is complicated by the high degree of functional redundancy of the signaling molecules involved Thus, the lack of...
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Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc
... dramatically decreased by the deletion of GC-box1 The activity was further decreased by the deletion of GC-box2 (Fig 3B) As shown in Fig 3C, the transcriptional activity of the kb of the 5¢-flanking region ... suppressed the expression of Sp1 and the antiSp3 shRNA-expression plasmid suppressed the expression of Sp3 The promoter activity of the NOX4 gene was significantly reduced by cotransfection with the anti- Sp3 ... disappeared in the presence of an anti- Sp1 IgG, while the supershift of the lower band in the presence of an anti- Sp3 IgG was depicted The binding specificity was evaluated with a 100-fold excess of unlabeled...
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![Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt](https://media.store123doc.com/images/document/14/rc/kt/small_AqRuqpZDo1.jpg)
Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt
... serum, the increase of c-Fos content in ouabain-treated HeLa cells was insensitive to the presence of the ERK inhibitor PD98059 (Fig 7C) These negative results are consistent with the lack of activation ... VSMCs, leading to the identification of mortalin as an antiapoptotic gene triggered by elevation of the [Na+]i ⁄ [K+]i ratio [7] Keeping in mind the increase of total RNA synthesis seen in ouabain-treated ... inhibitors of RNA and protein synthesis [6], suggesting the de novo expression of genes involved in suppression of the cell death machinery This hypothesis was confirmed by proteomics-based analysis of...
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Báo cáo khoa học: PKA independent and cell type specific activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx
... cotransfection of PKAc restores its PKA activity Finally we tested the effect of H-89, a known inhibitor of PKA, on the expression of both the proglucagon gene promoter and the Cdx-2 gene promoter in the ... shown in Fig 5, the effects of H-89 on these two promoters are apparently different At the concentrations of either 0.1 or lm, H-89 significantly repressed the basal expression of the proglucagon ... examinations were then conducted using the vehicle as the control for the longest time point Figure 3D shows the activation of forskolin ⁄ IBMX treatment on Cdx-2 protein expression in the gut endocrine...
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báo cáo hóa học:" Heterogeneous activation of the TGFβ pathway in glioblastomas identified by gene expression-based classification using TGFβ-responsive genes" pptx
... Cooperation of the ErbB2 receptor and transforming growth factor beta in induction of migration and invasion in mammary epithelial cells Proceedings of the National Academy of Sciences of the United ... from the scatter plot The average of the two replicates (OLIGO III 744, OLIGO III 744.1) was used for the same reason A total of 78 tumors from this dataset were used in the subsequent analysis The ... downregulated by TGFβ in another study, the direction counts will cancel each other during summarization therefore the total direction counts will be fewer than the total counts of the studies (Additional...
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Báo cáo hóa học: " Transcriptional responses in the adaptation to ischaemia-reperfusion injury: a study of the effect of ischaemic preconditioning in total knee arthroplasty patients" docx
... consisted of three five-minute periods of tourniquet insufflation on the upper thigh of the operative limb, interrupted by five minute periods of reperfusion The pressure to which the tourniquet ... carried out the experimental work including the extraction of RNA, validation of microarray data by real-time PCR and the analysis of serum samples The microarray experiment and the analysis of array ... patients were compared The analysis of gene expression patterns at the onset of surgery allowed for the identification of changes resulting from the precondition- Murphy et al Journal of Translational...
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Báo cáo sinh học: " HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system" doc
... knockdown of DCAF1, and then transduced cells with pHR-VPR Knockdown of DCAF1 did not affect the cell cycle profile of mock-transduced cells, but almost completely relieved the induction of G2 arrest ... immunoprecipitates obtained with anti- HA antibody (specific for HA-Vpr) were analyzed by WB for the presence of Flag-DCAF1 (Figure 3, panel A), the presence of a reactive band of the expected molecular ... of control fibroblasts These results, indicate that DDB2 is dispensable for the induction of G2 arrest by Vpr Thus, these results, together with the finding that Vpr binds to DCAF1, support the...
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Báo cáo sinh học: " Dengue virus serotype infection specifies the activation of the unfolded protein respon" pot
... proliferation through the activation of specific components of the Unfolded Protein Response [31,32] These observations are also supported by the occurrence of replication and maturation of flaviviruses ... determinant for the physiological consequences of infection Since the activation of the UPR is on the one hand essential for cell survival during viral infection and on the other hand detrimental ... determined whether the modulation of the UPR could have any impact on virus growth Salubrinal, a selective inhibitor of the protein complex (containing the protein phosphatase and its cofactor GADD34)...
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Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt
... cells of embryonic origins, the ethical issue regarding the source and safety of allo- and xeno-grafting has become important concern in the clinical setting On the other hand, the use of adipose-derived ... conical tube to which mL of saline was added to rinse the remaining cells through the filter The flow -through was pipetted into a new 50 mL conical tube through a 40 mm filter The tubes were centrifuged ... damage of renal parenchyma and destroy the architectural integrity of the kidney One important finding in the current study is that the mRNA expressions of TNF-1a and MMP-9 as well as the protein...
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Báo cáo hóa học: " HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system" pptx
... knockdown of DCAF1, and then transduced cells with pHR-VPR Knockdown of DCAF1 did not affect the cell cycle profile of mock-transduced cells, but almost completely relieved the induction of G2 arrest ... immunoprecipitates obtained with anti- HA antibody (specific for HA-Vpr) were analyzed by WB for the presence of Flag-DCAF1 (Figure 3, panel A), the presence of a reactive band of the expected molecular ... of control fibroblasts These results, indicate that DDB2 is dispensable for the induction of G2 arrest by Vpr Thus, these results, together with the finding that Vpr binds to DCAF1, support the...
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Báo cáo hóa học: " Dengue virus serotype infection specifies the activation of the unfolded protein response" pdf
... proliferation through the activation of specific components of the Unfolded Protein Response [31,32] These observations are also supported by the occurrence of replication and maturation of flaviviruses ... determinant for the physiological consequences of infection Since the activation of the UPR is on the one hand essential for cell survival during viral infection and on the other hand detrimental ... determined whether the modulation of the UPR could have any impact on virus growth Salubrinal, a selective inhibitor of the protein complex (containing the protein phosphatase and its cofactor GADD34)...
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