is in vitro fertilization ivf necessary

... recombinant luteinizing hormone is as effective as, but safer than, urinary human chorionic gonadotropin in inducing final follicular maturation and ovulation in in vitro fertilization 14 In Vitro ... chorionic gonadotropin may improve in vitro fertilization cycle outcomes in patients with low luteinizing hormone levels after gonadotropinreleasing hormone antagonist administration Fertility ... published April, 2012 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechopen.com In Vitro Fertilization...

Ngày tải lên: 16/03/2014, 21:21

... recombinant luteinizing hormone is as effective as, but safer than, urinary human chorionic gonadotropin in inducing final follicular maturation and ovulation in in vitro fertilization 14 In Vitro ... chorionic gonadotropin may improve in vitro fertilization cycle outcomes in patients with low luteinizing hormone levels after gonadotropinreleasing hormone antagonist administration Fertility ... published April, 2012 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechopen.com In Vitro Fertilization...

Ngày tải lên: 27/06/2014, 09:20

... maturation in vitro This time was also the beginning of the gynecologist’s interest in infertility It was in 1959 that the first Congress on Infertility was held in New York Five years earlier, in 1954, ... progress in IVF There was an explosion in the number of centers performing IVF in many countries, and it was also In Vitro Fertilization at this time that the first discussions on the ethics of assisted ... process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation Hum Reprod 1990; 5:7 23 Fleming R Gonadotrophin-releasing hormone agonist and estrogen–progestogen...

Ngày tải lên: 05/08/2014, 16:20

... having a lower fertilization rate The pregnancy rate is as good as other women having IVF Provided a GnRH agonist is used, the miscarriage rate is normal (14) Metformin, which lowers circulating ... circulating insulin levels, metformin may be an important aid in reducing ovarian hyperstimulation syndrome in these women SEMEN ANALYSIS WITH WHITE BLOOD CELLS STAIN AND CULTURE A semen analysis is ... more common in women who are clomiphene-resistant, that clinical group and insulin resistance may be particularly strong indications for this adjunctive treatment PCO women on metformin who are...

Ngày tải lên: 05/08/2014, 16:20

... protocol included a fixed day of GnRH-nt administration In the multiple-dose protocol, the antagonist GnRH-Antagonist in Human In Vitro Fertilization 77 Figure Gonadotropin-releasing hormone antagonist ... GnRH-Antagonist in Human In Vitro Fertilization 83 antagonists However, the optimal timing of the flexible approach of GnRH-nt administration is difficult since prediction of LH surge is difficult ... supplementation during GnRH antagonist administration in IVF/ ICSI cycles: a prospective randomized study Hum Reprod 2004; 19:1979–1984 GnRH-Antagonist in Human In Vitro Fertilization 89 73 Griesinger G,...

Ngày tải lên: 05/08/2014, 16:20

... before use in clinical treatment, even after installation and commissioning by the manufacturer, is very important Figure CO2 recordings from three incubators newly installed in the Assisted Conception ... QC schemes in the established IVF laboratory These include ensuring optimal laboratory performance is consistent and maintained Their use also allows rapid and effective troubleshooting should ... and the established IVF unit Benefits of Employing QA and QC Schemes When Setting Up a New IVF Laboratory When setting up a new IVF laboratory, or even making alterations to an existing laboratory,...

Ngày tải lên: 05/08/2014, 16:20

... may be an increased Monitoring In Vitro Fertilization Outcome 481 risk of an imprinting disorder following ART because of an association with infertility per se rather than with in vitro embryo ... malformations in stillborns, in terminations, and in live borns was 4.2% in ICSI and 4.6% in IVF (P ¼ 0.482) Outcome A superb series of papers on this cohort hindered only by the comparison group being IVF ... correction for gestational age Higher scores for singletons Matching not discussed in this letter Single observer 60% follow-up Monitoring In Vitro Fertilization Outcome 477 478 Sutcliffe Several...

Ngày tải lên: 05/08/2014, 16:20

... aberrations (including haploid and polyploid cells) in isolated inner cell mass (ICM) from bovine blastocysts cultured either in vivo or in vitro was quite high Moreover the incidence of embryos carrying ... reported an increased incidence of triploid IVF embryos (12.8%) in mice in comparison with those produced in vivo (2.7%) It is normal for polyploid cells to appear in trophoblast during early development ... h incubation oocytes were washed and placed into fertilization droplets (10 oocytes in 50 wL droplet) overlaid by paraffin oil (Merck) Before insemination, hypotaurine (4.6 pg/mL), epinephrine...

Ngày tải lên: 09/08/2014, 18:22

... was given intramuscular betamethasone (12 mg/d × days) and placed on bedrest At this point allergy injections were discontinued since the patient was not outdoors and risk for insect sting was ... the incidence of allergy to insect stings is ~3% in adults [3], with allergy to hymenoptera species venom comprising an important subset of this population Whether or not severe insect sting ... recognized independent risk factors for miscarriage In addition to venom protein, our patient received other medications which modify immune response including aspirin [15], heparin [16], progesterone...

Ngày tải lên: 13/08/2014, 13:22

... during in vitro fertilization (IVF) are maintained in extended culture to the blastocyst stage (4–5d post fertilization) when the polarized inner cell mass (ICM) develops The outer trophoectoderm is ... and Parkinson's Action Network, as well as the Cancer Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent ... prominently in the informed consent for patients contemplating PGD [22] Currently, a technique to determine the extent of embryo mosaicism without disassembling the embryo (and thus rendering...

Ngày tải lên: 13/08/2014, 23:20

... TNFa-induced U937 cell apoptosis by maintaining mitochondrial integrity and function [43], and ricin-induced apoptosis of U937 cells by maintaining an intracellular reducing condition by acting ... glucocorticoid Next, we investigated the existence and quantitative alteration of Mn-SOD and Cu/Zn-SOD proteins in chorion laeve tissue during in vitro incubation using Western blotting analysis With specific ... chorion laeve tissue during in vitro incubation Tissue was incubated in medium containing mM PDTC (A), 30 mM NAC (B), mM NDGA (C) or mM alloprinol (D) the upper and lower parts in each figure show...

Ngày tải lên: 22/02/2014, 07:20

... COS7-SPC99-3FLAG in vitro c-secretase activity While performing control reactions for inhibitor experiments, a two- to fivefold increase in the detection of products arising from in vitro c-secretase ... decrease in c-secretase activity with increasing zinc content The quantitated data is shown in graphical form under each panel strategy for the prevention of AD An initial step in discovering c-secretase ... within Ab constitute a domain that binds metal ions such as zinc, copper, and iron and mediates Ab aggregation ([23] reviewed [38]) This is the first report suggesting that this metal binding...

Ngày tải lên: 16/03/2014, 23:20

... some protein kinases are involved in stress signaling pathways [29] and recent work points towards the involvement of Ca2+/CaM-dependent protein kinases in stress signaling [16], changes in the ... Biochem 269) binding with DNA [27,53–55] We have found that the binding of p40 is dependent on the dephosphorylation status of the protein and the binding is fully abolished once the protein is phosphorylated ... 269) of this class of kinase are limited in plants [17–21] The Ca2+/CaM kinase isolated from maize roots has been shown to be involved in gravitropism [19] and the Ca2+/ CaM kinase isolated from...

Ngày tải lên: 18/03/2014, 01:20

... activator protein (lane 11), indicating that the Mediator is involved in activated transcription Neither the inclusion of human recombinant TFIIA nor the Gal4 DNA binding domain had an effect ... centrifugation, and the insoluble pellet containing the recombinant protein was washed twice with buffer A containing 0.05% (v/v) sodium deoxycholate and 0.1% (v/v) Triton X-100 Recombinant proteins were extracted ... of transcription were obtained in the whole-cell extract without (lane 1) or with (lane 2) Gal4 DNA binding domain and in the reconstituted assay with Gal4 binding domain (lane 3) We also tested...

Ngày tải lên: 30/03/2014, 14:20

... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining ... only in cells undergoing apoptosis) In brief, sections were blocked for endogeneous peroxidase as above, followed by addition of avidin/biotin and protein blocking using respective blocking kits ... were washed and incubated with 2’, 5’diaminobenzidine (DAB, Vector Laboratories, Burlingame, CA) followed by counterstaining with hematoxylin Caspase staining was performed using an Ab specific...

Ngày tải lên: 18/06/2014, 19:20

... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation ... the incorporation of uracil in their genomes These residues in DNA can arise either through misincorporation of dUTP by DNA polymerase or the spontaneous deamination of cytosine and can result in ... of administration and it is unclear if reduced virulence would be observed following intranasal inoculation We show here that there is little if any attenuation when mice are intranasally infected...

Ngày tải lên: 20/06/2014, 01:20

... evidence is needed to support this hypothesis and it would be interesting to investigate whether SCH 16 is indeed a cap dependent translation inhibitor Conclusion In conclusion, the findings of this ... post-infection thereby indicating inhibition of viral protein synthesis In order to ascertain whether this was indeed also true for SCH 16 we adopted another approach to investigate the precise ... of the effects of isatin, an endogenous MAO-inhibitor, and seleginine on bradykinasia and dopamine levels in a rat model Parkinson's disease induced by the Japanese encephalitis virus Neurotoxicology...

Ngày tải lên: 20/06/2014, 01:20

... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining ... only in cells undergoing apoptosis) In brief, sections were blocked for endogeneous peroxidase as above, followed by addition of avidin/biotin and protein blocking using respective blocking kits ... were washed and incubated with 2’, 5’diaminobenzidine (DAB, Vector Laboratories, Burlingame, CA) followed by counterstaining with hematoxylin Caspase staining was performed using an Ab specific...

Ngày tải lên: 20/06/2014, 03:20

... cytochrome P450 gene (CYP11A1) revisited: binding of GATA, cyclic adenosine 3', 5'-monophosphate response element-binding protein and activation protein (AP)-1 proteins to a distal novel cluster of Page ... Tnfaip6; tumor necrosis factor αinduced protein 6; Ptx3: pentraxin 3; PGE2: prostagrandin E2; PTGS2; prostagrandin synthase 2; AREG; amphiregulin; EREG: epiregulin; BTC: β-cellulin; EGFR: EGF receptor; ... signaling pathways induced by gonadotropin overlap the EGF-like factor-EGFR pathway, which induces full cumulus expansion and oocyte maturation In this study, to clarify the intracellular pathway involved...

Ngày tải lên: 20/06/2014, 07:20

... Marshall GM: The cyclin-dependent kinase inhibitor, p21 (WAF-1), promotes angiogenesis by repressing gene transcription of thioredoxin-binding protein in cancer cells Carcinogenesis 2009, 30(11):1865-71 ... Total dose dominates responses of this cell lines and sparing by fractionation is not as effective as other cell lines Surprisingly the most Page of 14 resistant cell line U251 is unexpectedly ... radioresistant glioblastoma cells [23,24] express diminished p21 in vivo We caution that we have certainly not proved that diminished p21 expression in vivo is the mechanism that underlies increased...

Ngày tải lên: 09/08/2014, 09:20