irbp is essential for normal retinoid processing in conephotoreceptors

... detected in the NOP53 strain after of chase, in Dnop53 ⁄ A-NOP53 7S pre-rRNA was still visible after 60 min, showing a defect for processing 27S into 5.8S 4453 RNA processing in S cerevisiae D ... protein interacting with Nop17p, which is involved in the early steps of prerRNA processing [28] Nop17p and Nop53p interacted in the two-hybrid system independently of the tag, but the interaction ... reported in the global yeast protein localization program [32] The interaction with Nip7p indicated that Nop53p is involved in the late steps of rRNA processing Evidence supporting this hypothesis...

Ngày tải lên: 20/02/2014, 01:20

... fighting further wars to destroy these men, who have been responsible for the greatest sufferings in the history of the world Human ambition, however, is indifferent to the lessons of history In ... and it is in such moments that men also discover their own physical strength, and other advantages of resistance For instance, a man who is attacked by an animal will fiercely in an effort to ... nations to prevent wars in the future Their leaders have met at various times, in the course of history, to discuss peace and to find a way of removing the causes of war It is this urge that led to...

Ngày tải lên: 15/07/2015, 23:19

... giống Trong số lượng lớn trường hợp, có khác biệt đua, ngôn ngữ tôn giáo nhân dân Đây kết nhu cầu kinh tế xã hội thời đại, có người buộc phải rời bỏ nhà cửa ban đầu họ sống người gốc nước Những đa ... đòi hỏi phải trì thái độ khoan phần người dân Họ phải tôn trọng thói quen văn hóa, xã hội niềm tin kiềm chế khỏi làm, nói điều mà xúc phạm đến tính nhạy cảm số người Các vụ xung đột sắc tộc tôn ... tội trở thành nạn nhân tàn ác vô tình Khoan dung điều cần thiết cho hòa bình hòa hợp quốc gia minh họa cách tham chiếu tới người Malaysia, quốc gia mà người dân thuộc chủng tộc khác cư trú Mặc...

Ngày tải lên: 27/08/2016, 08:17

... The formation of the intermediate by the two serine single-mutant proteins was almost complete within the dead time of the instrument and indistinguishable from that of the wild-type in single-turnover ... synergistic effect This result is consistent with the inability of the double-mutant protein to form the transient flavin intermediate, which normally forms before any bond-breaking steps occur [18,19] ... mutagenesis study to test mechanistic proposals In our first study we demonstrated that the invariant histidine residues (His17 and His106) function as general acids in the active site, with His106...

Ngày tải lên: 07/03/2014, 05:20

... the ATP-binding domain of HSP70 is critical for sequestering AIF in the cytosol [29] In the present study, we demonstrated that the ATP-binding domain of HSP70 was indispensable for inhibition ... mechanism is independent of the interaction of HSP70 with Smac but requires the ATP-binding domain of the protein However, it ATP-binding domain of HSP70 inhibits Smac release remains to be determined ... Jiang et al The ATP-binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore,...

Ngày tải lên: 16/03/2014, 01:20

... wild-type proteins arguing against a general misfold of the proteins caused by the mutations Discussion The CXXC motif at the reaction centre is indispensable for the interaction with FAD The interpretation ... genetic evidence for an essential involvement of the N-terminal cysteine pair in the in vivo function A possible function of this N-terminal CXXC motif in Erv1p is suggested by the discovery of a ... bacterial dsbB protein in the periplasm also contains two cysteine pairs acting in concert for the transfer of disulfides to dsbA [25] Interestingly, the redox function of dsbB is linked to the bacterial...

Ngày tải lên: 17/03/2014, 10:20

... exists on the role of this protein in the biogenesis of heme-containing proteins in plants To gain insight into this process, we decided to study the role of frataxin using the enzyme catalase as ... of frataxin in iron metabolism, remain unclear, especially in photosynthetic organisms Iron as a cofactor is involved in many cellular processes: (a) biogenesis of Fe–S proteins accomplished by ... (1997) Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants EMBO J 16, 4806–4816 Grinberg LN, O’Brien PJ & Hrkal Z (1999) The effects of heme-binding proteins on the peroxidative...

Ngày tải lên: 28/03/2014, 23:20

... IGFBP-7 for insulin [6] Interestingly, Imp-L2 has been shown to bind human insulin, IGF-I, IGF-II and proinsulin, and its homolog in the moth Spodoptera frugiperda, Sf-IBP, can inhibit insulin signaling ... with insulin for binding to the insulin receptor (InR) and inhibit the autophosphorylation of InR [6] Furthermore, IGFBP-7 is suspected to be a tumor suppressor in a variety of human organs, including ... Results Insulin/insulin-like growth factor (IGF) signaling (termed IIS) is involved in the regulation of growth, metabolism, reproduction and longevity in mammals [1-3] The activity of IIS is regulated...

Ngày tải lên: 06/08/2014, 18:21

... overexpressed in colon carcinomas [21,22] In the observations of Collins et al [23], MMP-2 mRNA is more significantly expressed in tumor lesions than in normal colon tissues Immunostaining assay ... of proteins by immunoblotting assay Phosphorylation of JNK1/2 was significantly induced within 15 in response to PGE2 stimulation, and was maintained up for 24 h (Figure 3B) The findings suggested ... regulated kinase; p38 MAPK: p38 mitogen-activated protein kinase; JNK: c-Jun Nterminal kinase; NF-κB: nuclear factor κ B; PI3-K: phosphatidylinositol 3-kinase; PKB: protein kinase B; uPA: urokinase...

Ngày tải lên: 10/08/2014, 10:20

... glutathione depletion in pollen grains triggered disturbances in auxin metabolism which are linked with inhibition of pollen germination induced by BSO treatment Conclusions Summing up, it can be ... pollen grains of the pad2-1 mutant, in which glutathione synthesis is distorted by a single point mutation in GSH1, decreasing glutathione content to ~20% of wildtype [32] a similar result is observed ... synthesis or an existing glutathione pool available in the Page of 11 pollen grains before the start of germination The subcellular distribution of glutathione will also reveal if glutathione is distributed...

Ngày tải lên: 11/08/2014, 11:21

... used in performing forward genetic screening The intrauterine development of mouse fetus is inconvenient for the observation of phenotypes in embryonic stages In addition, liver is the initial ... lots of serum proteins, such as albumin, fibrinogen, prothrombin, as well as protein C, protein S and antithrombin These substances are extremely essential in maintaining homeostasis of the body ... essential functions, including storage of substances, maintaining homeostasis, blood detoxification, producing numerous enzymes for metabolism, and serving as an endocrine/exocrine organ In mammalian...

Ngày tải lên: 14/09/2015, 12:11

... Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis Nat Cell Biol 6, 207–214 Kosinski PA, Laughlin J, Singh K & Covey LR (2003) A complex containing polypyrimidine tract-binding ... The apoptosis assay was performed using an ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Chemicon), following the manufacturer’s instructions AP staining was performed using an AP ... insights into gene regulation through mitosis Another interesting approach would be to clarify the significance of PTB in cells without a mitotic cycle Heart and brain tissues may be interesting...

Ngày tải lên: 07/03/2014, 00:20

... Thirdly, the C-terminal 49 amino acids are essential for the dimer formation [24] Fourthly, the C-terminal domain of HSP90 contains a client-binding site with characteristics distinct from those ... region is truly indispensable for client binding or simply present adjacent to the client-binding site with the result of being affinity-labeled with the client peptide This issue is now under investigation ... Bearing in mind the fact that the 35-amino-acid residues corresponding to the C-terminus of HSP90 are deleted in HtpG, we postulated that the regions within the C-terminal domain responsible for...

Ngày tải lên: 23/03/2014, 20:22

... từ đó) It is time for us to check in for our flight 2 Các bạn di chuột vào cụm từ để biết chức cụm câu: It is time for us to check in for our flight 3 Tại câu lại dịch vậy? - “It is time” – ... *It is time for us to check in for our flight Hình thức cấu trúc ngữ pháp: “It’s time (for somebody) to something” - đến lúc người làm việc Chúng ta quan ... việc Chúng ta dùng cấu trúc để phê phán hay phàn nàn Ví dụ "It is time for us checked in for our flight It is long after the checking time (Lẽ nên làm thủ tục cho chuyến bay Đã làm thủ tục lâu...

Ngày tải lên: 25/03/2014, 03:20

... towards gaining an insight into why RecD is essential in P syringae, we have analyzed the in vitro biochemical activities of this protein We report here the comparative activities of the C-terminally ... also similar in their domain architectures, each containing three distinct domains (domains 1–3) Domain (residues 159–417) and domain (residues 418–682) of P syringae RecD, corresponding to homologous ... was initially expressed as a C-terminally hexahistidinetagged protein from the high-level expression vector pET21D-His in E coli, in which it formed inclusion bodies Therefore, the protein was...

Ngày tải lên: 30/03/2014, 04:20

... similar but distinct cysteine- and histidine-rich (CHORD) Zn2+-binding domains and was proposed to play a role in stabilizing R proteins in a confirmation that is implicated in receiving pathogen ... to study the mechanism of broad-spectrum and rate-limiting disease resistances An understanding of the underlying mechanism of this type of resistance is important for developing strategies to ... protein in disease resistance signaling was first recognized in barley through Mla12-mediated resistance against powdery mildew [8] The same role for RAR1 has since been identified in the resistance...

Ngày tải lên: 12/08/2014, 05:20

... biosynthesis is constitutively localised in plastids For details on Arg biosynthesis up to Orn see [3] ASL: argininosuccinate lyase; ASSY: argininosuccinate synthetase; OTC: ornithine transcarbamylase, ... kinase can be alleviated by the plastidic PII protein, but the precise role of this interaction in regulating Arg biosynthesis is yet unknown [42] The block in mitochondrial Arg catabolism in ... (The Bio-Array Resource for Arabidopsis Functional Genomics [29]) Abbreviations Arg: arginine; ASL: argininosuccinate lyase; ASSY: argininosuccinate synthetase; Cit: citrulline; Dw: dry weight;...

Ngày tải lên: 12/08/2014, 05:20

... the mucosa for incoming antigens Moreover, in response to LPS or HDM, TLR4+ SCs produced DCactivating cytokines such as GM-CSF in the airways This cytokine is likely to be involved in airway DC ... pathways exists in response to LPS in TLR4-mediated signalling [21], whereas a third pathway independent of TLR4 possibly exists [22] Noulin et al demonstrated that MyD88 is indeed essential for the ... cytokines by mediastinal lymph node T cells Interestingly, in the same mice, the levels of instructing cytokines were severely impaired Interestingly, inhalation of a TLR4 antagonist to target ECs...

Ngày tải lên: 12/08/2014, 14:20

... agents inducing tetherin to achieve significant beneficial effects In vitro this can be achieved by treatment of human cells with interferon-alpha [6,20] Interestingly, interferon-alpha is upregulated ... out in 96-well-dishes in a volume of 100 μl and infected after overnight incubation with virus stocks containing ng of p24 antigen Three days post-infection viral infectivity was detected using ... site in Vpu is not critical for effective viral spread and CD4+ T-cell depletion in ex vivo infected lymphoid tissue It has previously been established that HIV-1 replication in HLT occurs mainly...

Ngày tải lên: 12/08/2014, 23:22

... PBM is essential for IL-2-independent growth of CTLL-2 cells induced by Tax1 PBM is essential for IL-2-independent growth of CTLL-2 cells induced by Tax1 (A) CTLL-2 is a mouse T-cell line This ... chimeric proteins indicated that the PDZ domain-binding motif (PBM) located at the Tax1 C-terminus, S/TXV (S/T, serine or threonine; X, any amino acid; V, valine), is responsible for the high ... hypothesis that PBM is a factor responsible for the distinct pathogenic activities of HTLV-1 and HTLV2 Since HTLV-1 is a T-cell-tropic virus, in this study, we examined the activity of Tax1 PBM in...

Ngày tải lên: 13/08/2014, 09:21