ionization and x ray production cross sections

... decreased in the series b-d-xylohexaose (Xyl6) > b-d-xylopentaose (Xyl5) > b-d-xylotetraose (Xyl4) > b-d-xylotriose (Xyl3), with no detectable afnity for b-d-xylobiose (Xyl2), which is consistent ... (Da)a TRX I (none) None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me 19046.920 n.b 19508.080 19656.081 19804.091 ... FEBS J Janis et al ă Thioxylo-oligosaccharide binding to xylanases Fig MS titration curves for TRX I (5 lM), TRX II (10 lM) and CTX (10 lM) with S-Xyl5-Me, S-Xyl4-Me and S-Xyl3-Me (initial concentrations...

Ngày tải lên: 07/03/2014, 17:20

... scanning calorimetry (DSC); X- ray powder diffraction (XRPD); combined, simultaneous, and dynamic differential scanning calorimetry /X- ray powder diffraction (DSC/XRPD); and high performance liquid ... of Mixtures of Polymorphs and Potential New Solids Forms By using the combined and dynamic DSC/ XRPD, several mixtures of disodium salt samples were identified, and the components of these mixtures ... mixture of hydrated Forms I and IV (sample 8) Figure 31 DSC of mixture of Form III monohydrate and Form IV hydrate (sample 56) Figure 32 Superimposed XRPD of mixture of Form III monohydrate and...

Ngày tải lên: 14/02/2014, 03:20

... residues 29, T34 and E131 are shown The hydrogen bonds between N29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein are shown as green broken lines, and the ion ... the protein, and a and b represent the a helix and b strand, respectively The side-chains of the mutated and parent amino acid residues at the six mutation sites are shown D39 ⁄ G39 and M76 ⁄ V76 ... 6·-RNase HI and wild-type proteins (full line) and between molecules C and D (broken line) a Helices and b strands are indicated by bars helices, and around Gly128 in a loop between the aV helix and...

Ngày tải lên: 18/02/2014, 13:20

... 164 x, y,z x +1,y,z x +1,y,z 1 -x, y +1 ⁄ 2,-z x, y,z x, y,z x, y,z x, y,z x- 1,y,z 1 -x, y-1 ⁄ 2,1-z x, y,z x, y +1,z x, y +1,z x, y-1,z x, y-1,z x, y,z 1 -x, y-1 ⁄ 2,1-z x, y,z 1 -x, y +1 ⁄ 2,-z x, y +1,z x, y,z x, y-1,z ... x, y-1,z x, y,z x, y-1,z 3.024(4) 2.783(5) 3.016(4) 3.138(5) 3.021(5) 2.776(5) 3.273(5) 3.122(5) 2.814(4) 2.848(5) 3.221(4) 2.767(5) 3.083(5) x, y,z x, y,z 1 -x, y-1 ⁄ 2,-z 1 -x, y-1 ⁄ 2,-z x, y,z x, y,z x, y,z ... characteristics of EM2 and EM2OH in DMSO, TFE, H2O (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2) Open and fold represent the conformations rS and n7 in parentheses indicate the reverse S- and numerical...

Ngày tải lên: 30/03/2014, 20:20

... Crystallography 39 a X X NH X = C,N,O X = C,N O O O O N H O X Y Y X N N NH C=O C O C C NH Y X b C=O NH C C O NH C X Y Y N X H N H N O N Pyrrole Imidazole Furan Benzene N N H N H N N N Pyridine Cyclohexane Pyrimidine ... trials Drug and latest reported development Company Target Phase PLX-4032 Plexxikon B-Raf V600E Phase ABT 263 Abbott Bcl-2/Bcl-xL ABT 869 Abbott VEGF and PDGFR AT9283 Astex Aurora AT7519 Astex CDKs ... 36–45], and are also covered in more depth by Wyss and coworkers [46] 2.2.2 X- Ray Crystallography X- ray crystallography is covered in detail by Bauman et al [47], Davies and Tickle [48], and Hennig...

Ngày tải lên: 29/05/2014, 23:54

... effects ozone and drought stress, an experiment was conducted on tree species (Picea abies and Fagus sylvatica) planted directly into the soil, enclosed in open-top chambers and exposed to a soil ... plants were used and McConnaughay et al (1993) have shown that nutrient changes in such trees can be artifacts of the experimental conditions Experimental site and plant material experiment was ... made using an X- ray take-off angle of 45°, a measuring time of 100 s, a magnification of 000 for all examined tissues (stomata, epidermis, mesophyll, parenchyma and endodermis) and of 400 for...

Ngày tải lên: 08/08/2014, 19:21

... London was an industrial Xray machine It was water-cooled and could produce X- radiation continuously The tube assembly type was a Comet ceramic X- ray tube assembly MXR160/0.4–3.0 The tube generator ... 0.254 mm and window diameter was 50 mm Studies on Dry and Hydrated Cross- Linked Hydrophilic Copolymer 26 The industrial X- ray tube was used to irradiate copper, molybdenum, silver and tin targets ... X- ray photons from the tube pass through a mm diameter collimator towards the target The target atoms are excited causing them to produce XRF photons unique to the element of the target The XRF...

Ngày tải lên: 07/08/2014, 14:20

... lobular carcinoma and in-situ ductal carcinoma studied in this work is referred to as glandular tissue Therefore, it is expected different peak positions will exist for carcinoma and fibrocystic ... CRM, Hufton AP and Dance DR Breast cancer diagnosis using scattered X- rays J Synchrotron Rad 2000; 7: 348-352 Malden CH, Speller RD, et al A CdZnTe array for the detection of explosives in baggage ... (second edition) Philadelphia: Lippincott Williams and Wilkins 1999 Kidane G, Speller RD, Royle GJ, Hanby AM X- ray scatter signatures for normal and neoplastic breast tissues Phys Med Biol 1999;...

Ngày tải lên: 02/11/2012, 11:08

... backbone and side chain 13Ca and 13C¢ and 13Cb carbons are assigned to the extent of 97%, 93% and 93%, respectively (BMRB accession number 6940) [24] The H–15N correlation in the TROSY experiment ... form of nPS using X- ray crystallography, as described below A Solution and refinement of the structure using X- ray crystallography B The crystal structure of the pantoate–nPS complex was solved by ... density maps and facilitate further rebuilding and improvement of the molecular model, until no unexplained electron density remained, and the Rcryst and Rfree values converged at 18.9% and 24.4%,...

Ngày tải lên: 16/02/2014, 09:20

... when the complex is formed The ligands cellobiose, cellotetraose and cellohexaose were studied Results and Discussion The crystal structure of CtCBM11, the binding cleft and its ligand specificity ... in the X- ray structures of CBM4 and CBM17 complexed with cellopentaose and cellohexaose, respectively [13,23] The involvement of the tyrosine residues in the stabilization of the complex cannot ... two six-stranded anti-parallel b-sheets that form a convex side (b-strands 1, 3, 4, 6, and 12) and a concave side (b-strands 2, 5, 7, 8, 10 and 11) The concave side is decorated by the side chains...

Ngày tải lên: 18/02/2014, 17:20

... a model and the experimental data: N X IðSj Þ À Iexp ðSj Þ2 v2 ¼ ð8Þ N À j¼1 rðSj Þ where for each momentum transfer value, Sj, I(Sj) is the theoretical scattering, Iexp(Sj) is the experimentally ... solution X- ray scattering These pieces were modelled into the AA and BD dimers as extended polypeptide [26], and produced an improvement in the fit of the AA model at S values between 0.15 and 0.2 ... Kd and k were varied to fit the experimental data ð5Þ ð6Þ nm)1 With a 3-m sample–detector distance, useful data began at an S value of 0.04 nm)1 and the linear Guinier region extended to approximately...

Ngày tải lên: 22/02/2014, 07:20

... structure and unfolding upon replacing the axial Met ligand with a Lys in the M100K variant of cyt c-550 from P versutus We describe three X- ray structures, one of the ferric wild type (wt) and two ... substituents are extremely sensitive to the chemical nature of the axial ligands to the hemeiron [41] Upon replacing the native Met ligand with either an exogenous or protein-based ligand a change ... weeks and repeated dissolving of the crystalline networks a single dark red crystal suitable for X- ray diffraction was obtained grown from 0.1 m bicine pH 9.0 and 3.2 m ammonium sulfate X- ray diffraction...

Ngày tải lên: 07/03/2014, 17:20

... is known from X- ray structural analysis of wild-type cyt b5 [21] that the core consists of b-strand III (Tyr27–Leu32), b-strand II (Thr21–Leu25), b-strand I (Lys5–Tyr7) and a-helix I (Thr8– His15) ... stage A random sample of 10% of the X- ray data was excluded from the refinement and was taken as the test data set, and the agreement between the calculated and observed structure factors of the ... grateful to Prof Li-Wen Niu, Prof Mai-Kun Teng and Dr Xue-Yong Zhu of the University of Science and Technology of China for their support and help with the X- ray data collection 18 REFERENCES Spatz,...

Ngày tải lên: 08/03/2014, 10:20

... superfamily of metzincin which exhibits some typical structural features, such as the Met-turn and active-site consensus HExxHxxGxxH sequence [15–17] Some organisms and mammalian tissues recently ... Huber, R & Bode, W (1995) X- ray structures of human neutrophil collagenase complexed with peptide hydroxamate and peptide thiol inhibitors Implications for substrate binding and rational drug design ... off by a solvent mixture of trifluoroacetic acid and ethanedithiol, and solvent was evaporated to dryness The resins were then washed with cold ether and the peptides were extracted with 5% acetic...

Ngày tải lên: 08/03/2014, 23:20

... Docking experiment with androstenol and androstenone The previously identified natural ligands androstenol and androstenone have been built with the program ACD/CHEMSKETCH [18] The parameter and topology ... Stereoview of SAL X- ray structure in ribbon representation The helix is in red, strands are blue and turns or coil are yellow The GlcNAc residue bound to Asn53 is in white ball -and- stick, and the serendipitous ... mouse MUP [21] and hamster aphrodisin [22] The residues interacting with androstenol (#) and androstenone (§) are identified by crosses, the letters i and o identify the positions ÔinÕ and ÔoutÕ,...

Ngày tải lên: 17/03/2014, 17:20

... residues Glu35 and Asp52 (numbering for HEWL), for BSL2 and other lysozymes, with propka 2.0 [16] The predicted pKa values were 6.15 and 4.27 for BSL2, 5.93 and 4.20 for HEWL, and 4.89 and 3.84 for ... acetate (for pH and 5) and sodium phosphate (for pH and 7) buffer The ionic strength of each buffer was adjusted to 0.1 [40] Lysozyme solution and M lysodeikticus suspension were mixed, and the decrease ... recombinant bovine stomach lysozyme (BSL2), the most highly expressed lysozyme in the cow stomach X- ray crystallography and some other experiments were performed to determine how this lysozyme has...

Ngày tải lên: 23/03/2014, 04:21

... LiOH provided the expected products PAH and FPAH as lithium salts in 28% and 15% overall yield, respectively X- ray crystallography Recombinant T brucei enolase was expressed and purified as previously ... 20 mm of ligand (PEP, PAH or FPAH) for and flash-cooled X- ray diffraction data were collected from two native crystals (referred to as sulfate_2 and sulfate_3 in Table 1) and four ligand-cocrystallised ... sulfate_2, PEP and PAH_1 structures is shown in Fig Unexpected variability in inhibitor complex structures Comparison of the substrate and inhibitor complexes shows that the His156-out and His156-in...

Ngày tải lên: 23/03/2014, 07:20

... 3¢-hydroxyl and 5¢-phosphate or 3¢-hydroxyl and 5¢-deoxyribose phosphate is recognized by Pol b, which fills the gap, removes the 5¢-deoxyribose phosphate and recruits XRCC1–DNA ligase IIIa complex to ... strand breaks containing 3¢-hydroxyl and 5¢-phosphate groups is accomplished by XRCC1–DNA ligase IIIa complex (pathway A) However, a singlenucleotide gap-containing strand break with 3¢-hydroxyl ... cell extract, XRCC1 or Pol b immunodepleted HeLa cell extract (B and E, respectively) for the times indicated and cross- linked with formaldehyde The beads were subsequently washed, the cross- links...

Ngày tải lên: 23/03/2014, 11:20

... central six-strand mixed b sheet (b2, b1, b3, b5, b6 and b4; b5 is antiparallel to the others) flanked by three a-helices (a1, a9 and a8) on the inner side and by two a-helices (a2 and a3) and two ... 1P74, 1WXD, 2EGG, 2HK7, 2HK8 and 2NLO), (b) binary complex bound with either cofactor (1NPD, 1NVT, 1NYT, 1O9B, 1P77, 1VI2 and 2CY0) or substrate (2D5C, 2GPT and 2O7Q) and (c) inactive (2HK9_A and ... amides of Asn58 and Asn85 The C4-hydroxyl group of shikimate interacts with the carboxylate group of Asp100 and with the side-chain amides of Asn85 and Lys64 The C3-hydroxyl group forms extensive hydrogen...

Ngày tải lên: 30/03/2014, 02:20

... f14 x; ZÞ ¼ f13 x3 ; ZÞ x ; x x x4 x3 where < f21 x; ZÞ ¼ a expðÀb1 x þ ðZ À aÞ expðÀcxÞ; x x1 x x x2 F2 x; ZÞ ¼ f22 x; ZÞ ¼ f21 x1 ; ZÞ exp½Àb2 x1 À x Š; : f23 x; ZÞ ¼ f22 x2 ; ZÞ exp½Àb3 ... calculate F (x, Z): x x1 > f11 x; ZÞ ¼ a expðÀb1 x þ ðZ À aÞ expðÀcxÞ; > > < f12 x; ZÞ ¼ f11 x1 ; ZÞ expðÀb2 x1 À x Š; x x x2 ð74Þ F1 x; ZÞ ¼ f13 x; ZÞ ¼ f12 x2 ; ZÞ exp½Àb3 x2 À x Š; x x x3 > ... (1996): X > < s1 x; ZÞ ¼ di ½exp x À 1Ši ; x xi S x; ZÞ ¼ i¼1 > : s2 x; ZÞ ¼ ½Z À s1 x; ZÞ À t2 Šg1 x þ t2 g2 x þ s1 x1 ; ZÞ x1 < x ð66Þ where g1 x ¼ À exp½t1 x1 À x Š and g2 x ¼ À exp½t3...

Ngày tải lên: 10/04/2014, 18:53