involvement of rh proteins in aminophospholipid movement

... transients are accentuated in pathological conditions, suggesting the involvement of astrocytic Ca2+ transients in brain disorders, including epileptic seizures Interestingly, astrocytes also show ... followed by the spreading of Ca2+ transients in adjacent astrocytes to form intercellular Ca2+ waves by a mechanism of chemical coupling mediated by ATP In both cases, an increase in [Ca2+]i is driven ... P2X7 receptors, suggesting the involvement of multiple pathways in the release In addition to these, the release of ATP is partly dependent on Ca2+ [15] and SNARE proteins [16], and astrocytes...

Ngày tải lên: 16/02/2014, 09:20

... by hematoxylin and eosin staining We examined the expression of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were ... cellular proteins but also in chromatin-bound proteins The Mcm proteins bound to chromatin probably function in DNA replication, but the role of the proteins not tightly bound to chromatin remains to ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa...

Ngày tải lên: 20/02/2014, 23:20

... derived from jararhagin disintegrin) is essential for binding to the I domain of a2 integrins [24] Similarly, members of the ‘lysine–threonine–serine (KTS)–disintegrin’ family contain the consensus ... a-amino group of the tissue inhibitor of metalloproteinases-2 NH2-terminal cysteine has been used to demonstrate the key role of this amino group in the inhibitory effect of tissue inhibitor of metalloproteinases-2 ... than RGD, in their integrin- binding loop [25,26] In addition, glycation of collagen lysine side chains is responsible for an impaired interaction of type I collagen with b1 integrins [27,28]...

Ngày tải lên: 07/03/2014, 06:20

... advantage of washed inverted vesicle preparations of Ms mazei, which contain all essential membranebound proteins involved in energy conservation and which are suitable for the generation of electrochemical ... lacking In this study, we present the first biochemical proof that Ech hydrogenase is indeed an ion-translocating enzyme, and thus represents an additional energy-conserving coupling site in methanogenic ... CO as the initial substrate When the oxidation of Fdred in the absence of CoMS-S-CoB was analysed in the washed vesicle prepara- Function of Ech in energy conservation tion, the rate of H2 production...

Ngày tải lên: 15/03/2014, 23:20

... Ca2+-binding EF-hand family protein Plastid movement impaired (PMI2) Ca2+-binding EF-hand family protein Protein kinase Zinc-finger (C3HC4-type RING finger) family protein Zinc-finger (C3HC4-type RING ... corresponding to the N-terminal fragment of either protein that retained the initiating Met in the presence or absence of myristic acid These results thus confirmed removal of the initiating Met ... 3, indicating that the initiating Met is retained in Myr– AGG1-3P6A and Myr–AGG1-3P6S This result is in good agreement with our previous finding of an inhibitory effect of Pro at position of full-length...

Ngày tải lên: 29/03/2014, 21:20

... arginines binding to the minor groove [50] This suggests that the weak DNA binding of the carboxyl terminus of CENP-C may be mediated by the arginines of the CENPC motif, with the remainder of ... DNAbinding proteins would maintain an interface with the conserved kinetochore machinery Indeed, regions of CENP-C that show evidence of positive selection include DNAbinding and specificity regions, in ... evolution of DNA-binding specificity in these regions Targeting of native CENP-C proteins depends on other centromere proteins that vary according to species [45], but the dependence of CENP-Cs...

Ngày tải lên: 06/08/2014, 18:21

... Conclusion This study provides insight into the involvement of oxidative stress in the pathogenesis of OA from the viewpoint of oxidative stress induced genomic instability, especially telomere ... Roles of chondrocytes in the pathogenesis of osteoarthritis Curr Opin Rheumatol 2002, 14:578-584 Martin JA, Ellerbroek SM, Buckwalter JA: Age-related decline in chondrocyte response to insulin-like ... measurable in the absence of interleukin-1 stimulation, presumably due to secretion of proteases in response to trauma The contribution of this basal catabolic activity could be minimized by culturing...

Ngày tải lên: 09/08/2014, 06:22

... conditions, IL-1β-induced apoptosis was increased in hypoxic chondrocytes lacking HIF-1α, to twice R909 Our findings show the potential involvement of HIF-1α expression in the progression of articular ... degeneration In patients with OA, stronger expression of HIF-1α mRNA in chondrocytes was observed in degenerating regions than in intact regions from the same articular cartilage samples Our findings in ... catabolic activity, including production of metalloproteinases [31,32] IL-1 also stimulates chondrocyte expression of inducible nitric oxide synthesis, iNOS, which results in an increase in NO production...

Ngày tải lên: 09/08/2014, 06:23

Ngày tải lên: 09/08/2014, 07:20

... stimulating an immune response in BD Most tissue studies of the pathophysiology of BD are conducted in skin One of the unique and diseasespecific features of BD is the occurrence of a positive skin ... elevated levels of IFN-γ, IL-12-p40 and IL-15; MIP3-α (macrophage inflammatory protein-3α); IP-10 (IFN-inducible protein-10); Mig (monokine induced by IFN-γ); iTac (interferon-inducible T cell ... Examples may include targeting cytokines (IL-1, IL-10, or IL-6) and use of agents that interfere with lymphocyte action Competing interests The authors declare that they have no competing interests...

Ngày tải lên: 09/08/2014, 14:20

... alleles of Indian Bos indicus into African populations, including sub-populations of the taurine type N’Dama, but the Kuri was not included in their study As a matter of fact, crossbreeding with ... variants of either bovine or caprine a The affected exons are exon in bovine a A [27, 38] and caprine -casein sl a,,-casein G [23], and exons 9, 10 and 11 in caprine a F [15] Still -casein sl another ... difference of 921.4 Da was indicative of a deletion of about eight amino acid residues RP-HPLC elution patterns of tryptic hydrolysates of a B and H showed the absence of one peak in a H as the -caseins...

Ngày tải lên: 09/08/2014, 18:21

... in saline at 37°C, washed × in saline and × in RPMI1640 containing 100 µg/ml penicillin and 100 U/ml streptomycin Worms were incubated with 10% gentamicin for 20 and then washed a further × in ... secreted proteins indeed acquired signal peptides, or have free-living lineages lost these motifs in the genes in question? Is more rapid diversification of secreted proteins a specific feature of ... stagnalis) NM_070558 RAS-like GTP-binding protein RhoA (Caenorhabditis elegans) Rhotekin (Mus musculus) NBC00297 4e-17 NM_009106 NBC00312 4e-46 A35350 Protein kinase C inhibitor (Bos bovis) NBC00269...

Ngày tải lên: 09/08/2014, 20:20

... activation of the serine/threonine mTOR protein kinase is involved in the inhibition of melanin synthesis in B16 melanoma cells [30] Because SPC triggers the activation of Akt, we Jeong et al Journal of ... quantifying the protein levels of the lysate and adjusting the protein concentrations with lysis buffer, 90 μL of each lysate containing the same amount of protein was placed in each well of a 96-well ... cells were incubated with SPC in the presence or absence of rapamycin, a specific mTOR inhibitor Addition of rapamycin significantly abolished the inhibition of melanin synthesis in SPC-treated...

Ngày tải lên: 10/08/2014, 10:20

... Involvement of lipid rafts in adhesion-induced activation of Met and EGFR Ying-Che Lu1 and Hong-Chen Chen1,2,3,4* Graduate Institute of Biomedical Sciences, National Chung Hsing University, ... determinant for integrin-dependent membrane rafts trafficking and regulation of growth signalling [13] In this study, we set out to examine the mechanism for adhesion-induced activation of Met ... EGFR using human epidermal carcinoma A431 cells, in which EGFR and Met are overexpressed and constitutively activated Possible involvement of matrix proteins, matrix stiffness, integrin β1, Src,...

Ngày tải lên: 10/08/2014, 10:20

... number of strategies to facilitate the implementation of research findings by using these social influences One strategy generating considerable interest is the use of opinion leaders Opinion ... examine the feasibility of identifying opinion leaders in different professional groups within the United Kingdom (UK) National Health Service using two different instruments, a sociometric instrument ... respondents Mean score of all respondents (SD) Range of scores of all respondents (SD) Total SDOLs Mean score of selfdesignating opinion leaders (SD) Range of scores of self-designating opinion leaders...

Ngày tải lên: 11/08/2014, 05:21

... plant nuclei contain multiple different SUN-domain proteins Models of the Topology of Plant SUN-domain Proteins The two structural classes of plant SUN-domain proteins found in maize, and shown ... these proteins reside or function in the NE remains to be determined In addition to their difference in size and SUN domain locations, these protein subfamilies are distinct in other interesting ... because of difficulty in the preservation conditions or in detecting the epitope in prophase nuclei or possibly because of an absence of PM3-type SUN-domain proteins in meiotic cells The results of...

Ngày tải lên: 11/08/2014, 11:21

... cellular proteins, including Actin, Keratin, Annexin, Coronin, Tubulin, Tropomyosin, and Cofilin Enveloped viruses acquire their envelope through budding from the host cell, thus cytoskeletal proteins ... ?-unknown proteins, macromolecular biosynthesis proteins, metabolism-associated proteins, calcium-dependent membranebinding proteins, glycoprotein, regualte apoptosis protein and other functional proteins ... guanine nucleotidebinding proteins integrate signals between receptors and effector proteins Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein interacts with IRS1 protein,...

Ngày tải lên: 12/08/2014, 01:22

... was partly due to induction of the gene in shoots GUS staining pattern of NaCl-treated roots seemed to be more complex GUS staining was indeed sharply reinforced in the terminal Renault et al ... mM NaCl before GUS staining (A) GUS staining pattern in shoots of plantlets (B-C) GUS staining pattern in roots of plantlets shown in A (D-E) Focus on root apices visible in B and C (F-G) Focus ... white boxes visible in B and C Arrows point to primary root nutrition of plant suggesting involvement of this isoform in nitrogen metabolism [37] Therefore, the increase of GAD2 expression at...

Ngày tải lên: 12/08/2014, 03:21

... cells cultured with d of TGFa and d of KGF Similar findings were observed in ATII cells cultured in KGF (K) alone, in d of K and d of KD (data not shown) or in d of KIA and d of KIAD (Additional ... production of mature SP-B Napsin A and pepsinogen C, asparatic proteases, are also involved in the processing of proSP-B [53,63] We found no changes in the protein levels of napsin A or pepsinogen ... its site of synthesis to the lamellar bodies [53,54] Cathepsin H is one of the cysteine proteases involved in the processing of proSP-B and proSP-C The first N-terminal processing step of proSP-C...

Ngày tải lên: 12/08/2014, 11:23

... self-perpetuating owing to the involvement of a host of redundant factors Such factors include various growth factors, proteases and inflammatory mediators that have been incriminated in the pathogenesis of ... population of interest, was prevalent in MCT-rats (p < 0.05 versus healthy rats) Interestingly, there was about 9-fold increase in the number of MCs around the intra-acinar vessels (20-50 μm in diameter), ... after MCT-injection The rats in healthy group received saline injection instead of MCT Double immunostaining for von Willebrand factor and a-smooth muscle actin, and elastica staining were performed...

Ngày tải lên: 12/08/2014, 13:22