involvement of foreign investment
Development of molecular diagnostics and antiviral therapy against enterovirus 71
Ngày tải lên :
12/09/2015, 08:20
... transcription of CA16 RNA 2.1.11.2 Conventional PCR 2.1.11.3 Cloning of the CA16 PCR amplicon 2.1.11.4 Preparation of CA16 RNA standards RNA interference of EV71 in vitro 2.2.1 Maintenance of RD Cells ... cause of herpangina which is an illness characterized by an abrupt onset of fever and sore throat, associated with the development of raised papular lesions on the mucosa of the anterior pillars of ... ii Table of contents iii List of Tables x List of Figures xi Abbreviations xvii Summary xviii CHAPTER LITERATURE REVIEW 1.1 1.2 Enteroviruses Enterovirus 71 1.2.1 Clinical Features of Hand, Foot...
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Tài liệu Báo cáo khoa học: "Reactive Content Selection in the Generation of Real-time Soccer Commentary" pdf
Ngày tải lên :
20/02/2014, 18:20
... of the solid line is higher than that of the dotted As before, the benefits or otherwise of this modification should be decided by comparing with 1286 Figure 6: Example of MIKE'S commentary of ... 4: An example transformation of importance of a proposition put can be is in English, French or Japanese To produce speech, MIKE uses off the shelf text-to-speech software English is produced ... development of the game situation In the Figure 5, we have illustrated this in the same way we did for the case of interruption The left hand side of the solid line is lower than that of the dotted...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
Ngày tải lên :
19/06/2014, 08:20
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of ... concentration of 0.5% Serial two-fold dilutions of virus suspensions were prepared in Alsever's buffer and 50 µL of viral suspension and an equal volume of RBC were added into each well of a 96-well...
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Báo cáo hóa học: " Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia" pptx
Ngày tải lên :
20/06/2014, 01:20
... the commonest cause of death in adult males in The Gambia [4] Detection of serological markers is the mainstay of diagnosis of HBV infection and the most reliable marker of HBV carriage is HBV ... quantification of virus load as a measure of replication of HBV in patients with chronic liver disease Prior to the development of the polymerase chain reaction (PCR) a number of hybridisation ... the withdrawal of LMV and this was more noticeable in the HBeAg positive group There was subsequent rebound of viral load at the withdrawal of LMV therapy Fluctuation of level of viral load was...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
Ngày tải lên :
20/06/2014, 04:20
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of ... concentration of 0.5% Serial two-fold dilutions of virus suspensions were prepared in Alsever's buffer and 50 µL of viral suspension and an equal volume of RBC were added into each well of a 96-well...
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báo cáo khoa học: " Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data" potx
Ngày tải lên :
12/08/2014, 03:21
... the evaluation of best suited reference gene(s) for normalization of qPCR data in a given set of biological samples Recognizing the importance of reference genes in normalization of RT-qPCR data, ... 10 μL of diluted cDNA (1:50), 0.2 μM of each primer, 50 μM of each dNTP, 1× PCR Buffer (Invitrogen), mM MgCl2, μL of SYBR®Green I (Molecular Probes) water diluted (1:10000), and 0.25 units of Platinum ... GhUBQ14 Table Values of efficiency ± standard deviation (SD) of the primers of the housekeeping genes and average values of quantification cycle (Cq) ± standard deviation (SD) of biological replicates...
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Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
Ngày tải lên :
12/08/2014, 04:21
... Viruses Sixteen strains of dengue viruses, including five strains of DENV-1, four strains of DENV-2, three strains of DENV-3, four strains of DENV-4 and one strain each of JE, WN and *Strains ... tool to investigate the role of viremia in pathogenesis of dengue Another important aspect of dengue disease is the surveillance of vector population and detection of DENV in field caught mosquitoes ... evaluate the specificity of the qRT-PCR Primer and probe design The 3'UTR of four strains of DENV-1 (nt 10230–10700), four strains of DENV-2 (nt 10301–10708), two strains of DENV-3 (nt 10243–10689),...
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Báo cáo y học: "Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection" pptx
Ngày tải lên :
12/08/2014, 23:23
... storage of samples, and the type of cells used In addition, the technique has a turnaround time of several days to weeks, and is therefore unable to guide initial patient management The results of ... mixture contained 80 µl of eluted RNA, 20 µl of 10 × RT buffer, 5.5 mmol/l MgCl2, 500 µmol/l of each deoxynucleoside triphosphate, 2.5 µmol/l random hexamer and 0.4 U of RNase inhibitor per microlitre ... 300–900 nmol/l of the forward and reverse primers and 75–200 nmol/l of each of Available online http://ccforum.com/content/10/2/R61 Table Demographic and clinical characteristics of children with...
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Báo cáo y học: "The effect of real-time CPR feedback and post event debriefing on patient and processes focused outcomes: A cohort study: trial protocol" ppsx
Ngày tải lên :
13/08/2014, 23:20
... performance category of patients Page of 17 at discharge The process focussed outcomes focus on both quality of CPR and team factors Quality of CPR outcomes are: quality of 2222 call; chest compression ... Cooke (Professors) Ben Abella is Clinical Research Director, Center for Resuscitation Science, University of Pennsylvania Nigel Stallard is Professor of Medical Statistics in the Division of Health ... Collaborators Joyce Yeung, Heart of England NHS Foundation Trust and University of Warwick Teresa Melody, Heart of England NHS Foundation Trust Page 12 of 17 Naheed Akhtar, Heart of England NHS Foundation...
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Báo cáo y học: "Base relative quantification framework and software for management and automated analysis of real-time quantitative PCR data" potx
Ngày tải lên :
14/08/2014, 17:22
... formulas N, number of replicates i; g, number of genes j; c, number of IRCs m, m'; r, number of runs l, l'; s, number of samples k; f, number of reference genes p, p'; h, number of standard curve ... run and run Fold change is defined as the ratio of the geometric mean of the (C)NRQs of the IRCs in run and run For the calculation of the effects of inter-run calibration, NRQ values were retrieved ... are used; formulas 21'-25') Calculation of the IRC stability measure allows the evaluation of the quality of the calibration, which depends on the results of the IRCs Our experiment shows that,...
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Improved symbolic model checking of real time systems
Ngày tải lên :
09/09/2015, 11:10
... number of processes, compared to the zone-based approach One disadvantage of digitization is that the number of reachable states of the digitized system is an increasing function of the number of ... condition, i.e., the set of accepting u locations An accepting run of A is a run which visits a state in Acc infinitely often The language of A over Acc, L(A), is defined as the set of accepting non-Zeno ... Then, the set of predecessors of a state r is defined as pre(r ) = {q ∈ S | q → r }, and the set of successors of a state r is defined as succ(r ) = {q ∈ S | r → q} The semantics of M is a transition...
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Interactive design space exploration of real time embedded systems
Ngày tải lên :
11/09/2015, 16:04
... Influence of sampling rates and bandwidth on the end-to-end delay (b) Influence of lengths of the static and dynamic segments on the end-to-end delay 142 List of Tables 2.1 dbf-table of ... Hardware/Software Partitioning We develop an efficient scheme for design space exploration in the context of hardware/software co-design of real-time systems Such systems nowadays consist of a heterogeneous ... “small” values of t, [9] constructs a new task graph by taking two copies of the task graph of T and adding an edge from the sink vertex of the first graph to the source vertex of the second and...
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Dynamic scheduling of real time control systems
Ngày tải lên :
04/10/2015, 17:06
... needed, which increases the workload of the computer Therefore, in design and implementation of digital control systems, there exists a trade-off between performance of a system and computational resources ... dependent on the trade-off consideration of scheduling constraints and control performance The performance is given as an index function of sampling frequency, and a set of optimal sampling frequencies ... concept of quality of service (QoS) is traditionally focused on performancecentric metrics, such as throughput, delay and jitter primarily in the field of data communication Recently, the use of this...
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Evaluation of real time methods for epidemic forecasting 2
Ngày tải lên :
12/10/2015, 17:34
... Effects of a on C(t) a=0.5 a=1 a=2 −5 Effects of K on I(t) 0.0 0.1 0.2 0.3 0.4 0.5 t Effects of r on I(t) r=0.5 r=1 r=2 0.1 0.2 0.3 0.4 K=0.5 K=1 K=2 0.0 I(t) 15 tm=0 tm=5 tm=10 −5 10 t Effects of ... −5 10 15 10 15 t Effects of a on I(t) a=0.5 a=1 a=2 0.2 0.1 0.0 0.0 0.1 0.2 0.3 10 t Effects of tm on C(t) −5 I(t) r=0.5 r=1 r=2 0.3 C(t) 0.0 0.2 0.4 0.6 0.8 1.0 Effects of r on C(t) 0.0 0.2 0.4 ... r=1 r=2 0.3 C(t) 0.0 0.2 0.4 0.6 0.8 1.0 Effects of r on C(t) 0.0 0.2 0.4 0.6 0.8 1.0 2.0 Effects of K on C(t) K=0.5 K=1 K=2 −5 ❢r♦♠ ❘✐❝❤❛r❞s ♠♦❞❡❧ ✇✐t❤ ♣❛r❛♠❡t❡rs ✉♥❧❡ss ♦t❤❡r✇✐s❡ st❛t❡❞ 0.5 C(t)...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
Ngày tải lên :
05/09/2013, 10:15
... Dr Fujimoto from Tokyo University of Agriculture ** Biofilm samples Samples of biofilm (10 g) were taken from a biological contact material, honeycomb catalyst, of a practical biological treatment ... reactions required µL of extracted DNA, 10 pmol of each primer, 30 pmol of TaqMan probe, 25 µL of TaqMan Gene Expression Master Mix (Applied Biosystems Co USA), made up to a final volume of 50 µL using ... microcystin-degrading bacteria in the biofilm of the biological treatment facility were quantified The bacteria were present in the biofilm throughout the year, suggesting that the biofilm of the biological treatment...
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