introduction to cell culture

... greatly to our knowledge and the fun of cell culture Finally, we would like to thank Dr Gordon Sato, who introduced us to the joy of cell culture and the infinite variety of interesting things to ... mammalian cell in vitro The Practice and Theory of Tissue Culture This book is meant to serve as an introduction to cell culture both for students who have little or no experience of cell culture ... any other) cell culture is to provide an environment that mimics, to the greatest extent possible, the in vivo environment of that specific cell type The cell culture incubator, the culture dish...

Ngày tải lên: 11/04/2014, 09:48

... Vero cells, Vero/ hSLAM cells and primary chicken embryo fibroblasts (CEFs) [9] Vero cells (African green monkey kidney cells) express a homologue of CD46 which serves as a receptor for cell culture- adapted ... effector cells to hours later, expression of β-galactosidase in cytoplasmic extracts was measured with a luminescent substrate (Clontech), following the manufacturer's Alternate Cell Lysis Protocol ... viruses were able to infect Chinese hamster ovary cells expressing the receptor CD46, indicating that they had not adapted to use CD46 as a receptor [9] The absence of a change in receptor usage indicated...

Ngày tải lên: 20/06/2014, 01:20

... window to that culture Although this Culture is important, what may be of more interest to students and potentially more useful to them, if they are going to interact with people from a new culture, ... English) Students are asked to identify main ideas or supporting details, to highlight topic sentences, to develop questions related to the main idea, to complete an outline, or to fill in a graphic ... more extroverted students are encouraged to reduce their output and to listen to their peers To these activities: Divide the class into small groups of three to six Identify the student who will...

Ngày tải lên: 03/06/2016, 09:07

... was to produce a document showing our work for the semester and also to make available to other students interested in fuel cells or taking an introductory fuel cell course We would like to thank ... the power companies themselves move toward a more hydrogen based from of electric power generation Thus as an introduction to fuel cells we need to study cell cells for two important reasons First ... Contents Preface Introduction 2.1 Fuel Cell Basics 2.2 History of Fell Cell Technology 2.3 Why are we studying Fuel Cells? 2.3.1 Why Fuel Cells are an Emerging...

Ngày tải lên: 23/04/2014, 22:24

... wt variant, with which it has to compete, and thus not given enough time to to establish a well balanced, mature quasi-species population We, therefore, decided to compare fitness of TULV02 with ... recTULV presents no real match to the original cell adapted variant and that the lower fitness of the recombinant virus can not be increased by pre-passaging in cell culture The observed survival ... passages was monitored by RT-PCR and the isolate appeared to have a stable genotype (data not shown) RecTULV formed foci similar in size to those of the original variant and grew to the titers ×...

Ngày tải lên: 18/06/2014, 22:20

... wt variant, with which it has to compete, and thus not given enough time to to establish a well balanced, mature quasi-species population We, therefore, decided to compare fitness of TULV02 with ... recTULV presents no real match to the original cell adapted variant and that the lower fitness of the recombinant virus can not be increased by pre-passaging in cell culture The observed survival ... passages was monitored by RT-PCR and the isolate appeared to have a stable genotype (data not shown) RecTULV formed foci similar in size to those of the original variant and grew to the titers ×...

Ngày tải lên: 20/06/2014, 04:20

... cylinder skewing and sector-based sparing with one spare sector per track This needs to be accounted for in mapping logical blocks to the physical sectors Adding all these factors results in the ... 97560 sector size 256 bytes 512 bytes cylinders 1449 1962 tracks per cylinder 19 data sectors per track 113 72 number of zones 1 track skew 34 sectors sectors cylinder skew 43 sectors 18 sectors ... specifications, since the only alternative is to determine them experimentally The information required to determine how much power to apply to the pivot motor and for how long on a particular seek...

Ngày tải lên: 12/09/2012, 14:16

... beginning to the end (for now)    No backing up to read something again (OK to start over) Just as done from the keyboard Writing to a file  Sending output to a file  Done from beginning to end ... fails, it is generally best to stop the program The function exit, halts a program  exit returns its argument to the operating system  exit causes program execution to stop  exit is NOT a member ... flag is an instruction to one of two options ios::fixed is a flag Will be written in fixed-point notation, the way we normally expect to see numbers Calls to setf apply only to the stream named...

Ngày tải lên: 12/09/2012, 22:49

... using complementary metal oxide semiconductor (CMOS) transistors They used no resistors and inductors, and the whole circuit was fabricated by the 20 INTRODUCTION very large scale integration ... may have to tune each of them to correct for manufacturing tolerances, but there is no such need to test the accuracy of the wordlength in digital filters Data on digital filters can be stored on ... control of the phone to the BTS in the next cell, which may offer a stronger signal If no such cell is nearby, the caller is cut off (i.e., will not be able to receive or to send ANALOG AND DIGITAL...

Ngày tải lên: 13/09/2012, 10:21

... software that helps identify other Bluetooth devices Bluetooth and Ultra Wide Band (continued) Bluetooth and Ultra Wide Band (continued) • Bluetooth – Distance: up to 33 feet (10 meters) – Bandwidth: ... (continued) Bluetooth and Ultra Wide Band • Radio frequency identification device (RFID) tags – Small chips containing radio transponders • Can be used to track inventory • Bluetooth and Ultra ... Used to transmit data over very long distance • Repeater – Located in the satellite itself – Simply “repeats” the same signal to another location – Used to transmit data from one earth station to...

Ngày tải lên: 13/09/2012, 10:52

... developed for a particular customer or may be developed for a general market Software products may be • •  Generic - developed to be sold to a range of different customers e.g PC software such ... systems that are intended to provide automated support for software process activities CASE systems are often used for method support Upper-CASE  Lower-CASE   • • Tools to support the early process ... engineers should adopt a systematic and organised approach to their work and use appropriate tools and techniques depending on the problem to be solved, the development constraints and the resources...

Ngày tải lên: 14/09/2012, 11:26

... If you're ready to make the jump to LVM and don't have a lot of space handy for allocating to LVM then it might make sense to reinstall your system The Debian installer has excellent support ... you really want to remove active logical volume "test"? [y/n]: y Logical volume "test" successfully removed Other userful commands include lvrename to change the name, and lvreduce to reduce the ... volumes What we really want to is create logical volumes which we can mount and actually use In the future if we run out of space on this volume we can resize it to gain more storage Depending on the...

Ngày tải lên: 18/09/2012, 10:12

... regulatory tools and techniques is to advocate combining techniques rather than relying upon any single instrument: an approach often referred to as a ‘regulatory toolbox’ approach As the toolbox ... invoking the expansion of choice available to citizens Yet both failed to give credence to the incapacity of particular sectors of the Introduction community to avail themselves of essential commodities, ... regulatory agencies and legislative actors in order to secure regulatory benefits, systems theorists might focus on the way in which the economic and political systems communicate (or fail to communicate)...

Ngày tải lên: 21/09/2012, 10:39

... philosophers of mind tend to distort or oversimplify the historical Descartes’s views, but this is not the place for me to engage with them over that issue 10 An introduction to the philosophy of ... about this Secondly, if they mean to abandon reasoned argument altogether, even in defence of their own position, then I have Introduction nothing more to say to them because they have excluded ... in which these topics are covered has been deliberately chosen so as to enable the reader to build upon the understanding gained from earlier chapters in getting to grips with the topics of later...

Ngày tải lên: 21/09/2012, 10:39

... feels obliged to take If to permit the expression of humanity is to tolerate the antisocial expression of youth power, this might also be to allow the rapists into your own home, into the writer’s ... to flee to the US to escape the totalitarian state that may result from the continuing appeasement of Hitler As a consequence, there is a mood of ‘dissolution’ in which private miseries seem to ... girlfriend he refuses to marry) convey an attachment to place that belies his stated intention to emigrate In this way Cooper manages to play two contexts off against each other: historical hindsight...

Ngày tải lên: 21/09/2012, 11:00

... franchisor and franchise system to the prospective franchisee An Introduction To Franchising THE IFA EDUCATIONAL FOUNDATION WHAT ARE THE ALTERNATIVES TO FRANCHISING? In addition to franchising, there are ... distributorships ✔ licensing PRODUCER EMPLOYEE PRODUCER/ SELLER PRODUCER/ LICENSOR INDEPENDENT DISTRIBUTOR LICENSEE PRODUCER/ FRANCHISOR FRANCHISEE CUSTOMERS In a distributorship, the distributor ... are similar to franchises, and vice versa A franchisee with a great deal of leeway in how to run the business may look like an independent distributor A distributor may be subject to many controls...

Ngày tải lên: 19/10/2012, 15:42

... but it is easy to imagine the course of progress from log to raft, from manual propulsion to sails, and from river to ocean navigation The Phoenicians and Egyptians built huge, excellent ships ... splendid artist, but he was an excellent scientist, too He was so well versed in the laws of natural science that he stated that ‘a body tries to drop down onto the earth through the shortest ... the history of the city water system is very old But in the development process of city water systems, in order to transport water effectively, the shape and size of the water conduit had to be...

Ngày tải lên: 22/10/2012, 10:59

... only too well known There are so many scientific terms named after Newton (Newton's rings and Newton's law of motion/ viscosity/resistance) that he can be regarded as the greatest contributor to ... 14.21 15.12 16.04 16.98 Stokes’ equation, to be stated in Section 9.3.3, is utilised for measuring the viscosity, it is named after him), while its 1/ 100th part is cSt (centistokes) Thus I s t = ... tension 13 sensitivity to temperature is expressed by the viscosity index VI,' a non-dimensional number A VI of 100 is assigned to the least temperature sensitive oil and to the most sensitive...

Ngày tải lên: 22/10/2012, 10:59

... (3.26) into (3.25) to calculate y, (3.27) Parallel a x b theorem: The moment of inertia with respect to any axis equals the sum of the moment of inertia with respect to the axis parallel to this ... to water pressure, such as the bank of a dam, the sluice gate of a dam or the wall of a water tank? How large must the torque be to open the sluice gate of a dam? What is the force required to ... metre Torr Pa bar mH,O atm mHg torr Pa = N/m2 bar = 0.1 MPa mH,O = 806.65 Pa atm = 101 325Pa mHg = 1/0.76 atm torr = 1mmHg 3.1.2 Absolute pressure and gauge pressure There are two methods used to...

Ngày tải lên: 22/10/2012, 10:59