... for 5 min to ensure complete denaturation of DNA ( 45 sec for subsequent cycles) followed by 45 sec at 57 °C (Cx40) or 51 °C (Cx43) to anneal primers, and then 1 min at 72°C for extension of ... different amounts of total proteins were tested for Western blot analysis. The amount of the protein was increased sequentially from 0 .5 μg, 1.0 μg, 1 .5 μg, 2.0 μg, 3.0 μg, 4.0 μg, 5. 0 μg, 6.0 ... 10.0 μg, 15. 0 μg, 17 .5 μg, 20.0 μg, 25. 0 μg and 30.0 μg. The optimal concentration was 4 μg for Cx43 and 20 μg for Cx40, respectively. The relative optical density of the Cx43 and Cx40 of different...
Ngày tải lên: 26/10/2012, 09:39
... a crucial role in adipogenesis FEBS Journal 2 75 (2008) 55 7 655 88 ê 2008 The Authors Journal compilation ê 2008 FEBS 55 77 serum. For determination of cell counts, cells were gently rinsed with ... obtained from RT-PCR, 5 RACE and 3Â RACE were subcloned into pBluescript fad49 plays a crucial role in adipogenesis T. Hishida et al. 55 84 FEBS Journal 2 75 (2008) 55 7 655 88 ê 2008 The Authors ... Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467- 8603, Japan Fax: +81 52 836 3 455 Tel: +81 52 836 3 455 E-mail:...
Ngày tải lên: 16/03/2014, 04:20
Development Tales of iPhone App Masters pot
... started this series of iPhone Projects books because we recognized that there is a community of iPhone developers all starting from scratch and full of enthusiasm for Apple’s iPhone and iPod touch ... BBPointMake(0.0, 0.0, -50 .0); particles.emissionRange = BBRangeMake( 250 0, 250 0); particles.emitCounter = 0.1; Here you are setting the emission range to fall between 2 ,50 0 and 5, 000 particles a ... Notified 43 The Tools of the Trade 44 Stop Talking, Start Coding! 45 Installing the Tools 45 Coding the Web Service 47 Accepting the Challenge on the Device 54 Making a Move 57 Summary 61 Tom Harrington...
Ngày tải lên: 17/03/2014, 23:20
Risk management in ERP project introduction: Review of the literature pot
... [ 152 ] J. Verville, A. Halingten, A six -stage model of the buying process for ERP software, Industrial Marketing Management 32 (7), 2003, pp. 58 5 59 4. [ 153 ] J. Verville, A. Halingten, ... in Industry 56 , 20 05, pp. 51 0 52 2. [53 ] M.J. Ginzberg, Early diagnosis of MIS implementation failure: promising results and unanswered questions, Management Science 27 (4), 1981, pp. 459 –476. [54 ] S. ... 1, 3, 4, 5, 6 [1 35] 54 . Empirical descriptive ERP implementation S-LC CSF 3, 4, 6, 15, 18 [56 ] 55 . Conceptual and empirical descriptive ERP implementation MS CSF + case study 1, 2, 3, 5, 6, 8,...
Ngày tải lên: 23/03/2014, 04:21
Engaging Russia as Partner and Participant- The Next Stage of NATO-Russia Relations pdf
... commitments of the NATO Treaty’s Article 5. 5 Yet, like other members of the EAPC/PFP, Russia is excluded from these aspects of NATO’s role as a nonsignatory of the treaty. ____________ 5 From ... fairly through official bureaucratic procedures. As a matter of fact, the designation of a state secret is an expression of the subjec- tive will of state officials within the framework of the power ... by other risks of a wider nature, including acts of terrorism, sabotage and organised crime, and by the disruption of the flow of vital resources. The uncontrolled movement of large numbers of people,...
Ngày tải lên: 29/03/2014, 20:20
Báo cáo khoa học: "DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION PROJECT" potx
... Structural transformation of an embedded sentence of type 3. 4 25 DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION ... the same as that of English. Fundamental difference from Japanese internal representation to that of English is absorbed at the (pre-, post -) transfer stages. But at the stage of English generation, ... gen- eration stages of Japanese-to-English system of the machine translation project by the Japanese Govern- ment, with the emphasis on the ideas to deal with the incompleteness of linguistic...
Ngày tải lên: 31/03/2014, 17:20
INTRODUCTION: ASSESSMENT OF SPEAKING AND LISTENING SKILLS IN ENGLISH (ASL) pptx
... against the walls of the cabin. All of us inside were quaking in our shoes. 8. The number of campers………………… 9. Place of camping…………………… 10. Intimidated by……………………… 11. Method of attack………………………………… ... outlined the significance of speaking and listening skills as part of co-scholastic areas of achievement by every learner and also stresses the clear integration of evaluation of speaking and listening ... Equipment used Quality of the recording of the Listening Input Quality of the oral rendering of the Listening Input (in case it is to be read aloud by the Examiner), in terms of volume, clarity,...
Ngày tải lên: 02/04/2014, 05:20
oreilly programming ios 5, fundamentals of iphone ipad ipod touch development 2nd (2012)
... . . . . 56 9 Creating a Scroll View 57 0 Scrolling 57 3 Paging 57 6 Tiling 57 7 Zooming 57 9 Zooming Programmatically 58 1 Zooming with Detail 58 1 Scroll View Delegate 58 4 Scroll View Touches 58 6 Scroll ... 53 7 Configuring a Tab Bar Controller 53 8 Navigation Controllers 54 0 Bar Button Items 54 4 Navigation Items 54 6 Toolbar Items 54 9 Configuring a Navigation Controller 54 9 Page View Controller 55 1 Container ... 51 7 Rotation 51 9 Rotation Events 52 1 Initial Orientation 52 3 Presented View Controller 52 6 Presented View Animation 53 1 Presentation Styles 53 2 Presented Views and Rotation 53 4 Tab Bar Controllers 53 6 Tab...
Ngày tải lên: 24/04/2014, 10:01
Báo cáo sinh học: " Cyclooxygenase activity is important for efficient replication of mouse hepatitis virus at an early stage of infection" ppt
... 4 :55 http://www.virologyj.com/content/4/1 /55 Page 2 of 5 (page number not for citation purposes) efficient replication of herpesviruses [7-13], bovine leuke- mia virus [14], and rotavirus [ 15] . ... 2007, 4 :55 http://www.virologyj.com/content/4/1 /55 Page 5 of 5 (page number not for citation purposes) 12. Ray N, Bisher ME, Enquist LW: Cyclooxygenase-1 and -2 are required for production of infectious ... fixed at 6 h post-binding. The numbers of infected cells are presented as described in the legend of Figure 1 (n = 3). 0 0 .5 1 2 4 0 25 50 75 100 1 25 * * * * P < 0.01 h post-binding relative...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Cyclooxygenase activity is important for efficient replication of mouse hepatitis virus at an early stage of infection" pptx
... 4 :55 http://www.virologyj.com/content/4/1 /55 Page 2 of 5 (page number not for citation purposes) efficient replication of herpesviruses [7-13], bovine leuke- mia virus [14], and rotavirus [ 15] . ... fixed at 6 h post-binding. The numbers of infected cells are presented as described in the legend of Figure 1 (n = 3). 0 0 .5 1 2 4 0 25 50 75 100 1 25 * * * * P < 0.01 h post-binding relative ... tests, P < 0. 05 was considered statistically significant. 0 25 50 75 100 1 25 * * Control Indometacin Curcumine * P < 0.001 relative % infected cells Virology Journal 2007, 4 :55 http://www.virologyj.com/content/4/1 /55 Page...
Ngày tải lên: 20/06/2014, 01:20
The 22 Immutable Laws of Marketing Introduction Billions of dollars have been wasted on marketing pot
... Japanese introductions of low-end small cars flanking the mid-range strength of GM. To find that singular idea, you must be intimately involved in the front lines of the marketplace. The Law of ... amount of flexibility into your organization to cope with the inherent unpredictability of the marketplace. The Laws of Success, Failure, Hype, Acceleration and Resources The Law of Success. ... supports the person who will personally benefit from a new project. The Law of Hype. The situation is often the opposite of the way it appears in the press. When things are going well, a company...
Ngày tải lên: 28/06/2014, 12:20
Báo cáo lâm nghiệp: "Quantity and distribution of fine root biomass in the intermediate stage of beech virgin forest Badínsky prales" potx
... 17.73 17 .53 5 10 cm 7.92 5. 52 19.37 15. 73 23.96 9. 95 34.84 15. 05 19.71 8.12 20.14 13.21 10–20 cm 7. 15 3. 95 5.60 4.36 16.13 7.37 12.92 6.13 9.93 5. 66 10.11 6 .55 20–30 cm 4. 85 5. 05 3.31 3.63 4. 75 3.26 ... 159 .2 692.8 658 .7 258 .5 1 05. 3 373.9 349.6 10–20 cm 1 95. 3 82.3 129.3 85. 7 2 05. 1 113.8 174.6 153 .1 173.8 128.9 1 75. 6 112.0 20–30 cm 70.6 46.9 51 .3 31.7 56 .1 26.3 21.9 9.4 51 .1 33.7 50 .3 33.6 30–40 ... 282.1 50 9.4 160.9 169 .5 38.7 33.0 140.8 156 .7 1 15. 6 43 .5 156 .1 254 .0 0 5 cm 204.3 136.6 228.3 153 .2 461.9 283.7 697.7 700.9 1 65. 0 83.2 361.6 397.8 5 10 cm 202.1 153 .3 313.9 118.9 377.8 159 .2 692.8...
Ngày tải lên: 07/08/2014, 10:22
Báo cáo lâm nghiệp: "Ultrastructural and biochemical changes at the preinfection stage of mycorrhizal formation by two isolates of Pisolithus tinctorius" ppt
... Micro- biol. Rev. 50 , 193-2 25 Horan D.P., Chilvers G.A. & Lapeyrie F.F. (1987) Time sequence of the infection process in eucalypt ectomycorrhizas. New Phytol. 109, 451 - 458 Lei J. ... response of eucalypts to mycor- rhizal inoculation in the Congo. For. Ecol. Manag. 24, 151 - 157 Ultrastructural and biochemical changes at the preinfection stage of mycorrhizal ... 65, 1927-1939 structural localization of polysaccharides and proteins, and measurement of acid phospha- tase activities. Results Four days after inoculation with strain 4 45, the...
Ngày tải lên: 09/08/2014, 04:20
Báo cáo y học: "Molecular profile of synovial fibroblasts in rheumatoid arthritis depends on the stage of proliferation" potx
... dehydrogenase B Nuclear factor of activated T cells 5 (NFAT5) Upstream of N-ras (unr) FBR 35 KIAA0143 335E3 KAT 052 23 RP1-187N21 KIAA0093 RP1-242G1 LOC510 65 A9A2BRB5 HP100 85 KIAA 156 2 KIAA0660 RP1-86D1 PLACE1001168 LDS6 LDS7 LDS8 LDS12 LDS14 LDS17 LDS34 LDS43 (a) (b) (c) Figure ... Rheumatic Diseases, Department of Rheumatology, Uni- versity Hospital Zürich, Gloriastrasse 25, CH-8091 Zürich, Switzer- land. Tel +41 1 255 57 37; fax +41 1 255 4170; e-mail: Steffen.Gay@ruz.usz.ch Supplementary ... 1. (a) (b) (c) FLB8331 HSPC241 HEP01486 TSAP19 KAIA2887 DKFZp586H 052 3 KAT082 45 RP11-48B24 RP11-467H10 KIAA1128 RP11-467P9 RP4 -54 1C22 KAT 056 59 HDS8 HDS12 HDS34 HDS37 HDS41 Glutamine synthetase Ribosomal...
Ngày tải lên: 09/08/2014, 06:22
báo cáo khoa học: " Increased IL-10 mRNA expression in tumorassociated macrophage correlated with late stage of lung cancer" pps
Ngày tải lên: 10/08/2014, 10:21
Báo cáo y học: " High-risk HPV E5-induced cell fusion: a critical initiating event in the early stage of HPV-associated cervical cancer" pptx
Ngày tải lên: 12/08/2014, 01:21