introducing the science of social psychology
Investigating the 2005 singaporean dengue outbreak
... and therefore suggesting that viruses 43 Introduction Investigating the 2005 Singaporean Dengue Outbreak of this genotype are mixed regardless of their location On the other hand, the picture of ... multiplication of the virus 1.3.2.3 Viral Replication, Assembly and Exocytosis Once the genome is released into the host cell, the positive sense RNA is transcribed into the polyprotein at the surface of the ... drug by the end of 2012 The main targets that are followed up are the E Glycoprotein, the NS3 protease, the NS3 helicase and the NS5 RNA dependent polymerase 1.2.3.4 Current Treatment of DF and...
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Báo cáo khoa học: " Phylogenetic studies reveal existence of multiple lineages of a single genotype of DENV-1 (genotype III) in India during 1956–2007" pdf
... makes the role of virus in pathogenesis all the more important The incursion of new genotype into an area is also being attributed to the severe form of the disease [19] All these facts drive the ... 2002 isolates The hydrophilic amino acids are found mostly on the surface of the protein and are involved in the immunological interactions The implications of these unique Page of (page number ... 1943; than to the other Indian sequences, with whom theses two sequences show a nucleotide identity of only 93% On carrying out the phylogenetic analysis of all these DENV-1, on basis of CprM sequences,...
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Purification and cloning of PCR products
... treating the DNA molecules with an enzyme such as T4 or T7 DNA polymerase in the presence of one dNTP determined by the design of the primers The 3′→5′ exonuclease (proofreading) activity of the DNA ... important source of difficulty is the terminal transferase activity of Taq DNA polymerase that leads to the addition of an additional nucleotide, usually an A, at the 3′-end of the newly synthesized DNA ... treat the DNA with a proofreading enzyme such as the Klenow fragment of DNA polymerase I, or T4 or T7 DNA polymerase, or a proofreading thermostable DNA polymerase, in the presence of the four...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... insufficient number of cells Accuracy The accuracy of an analytical method describes the closeness of mean test results (detected) obtained by the method to the true value (expected) of the analyte Accuracy ... guide for others in the field to validate these and other similar assays for assessment of patient T cell response These methods can be applied not only to cancer vaccines but to other therapeutic ... assessment) Precision The precision of an analytical method describes the closeness of agreement (degree of scatter) between a series of measurements obtained from multiple sampling of the same homogenous...
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Báo cáo y học: " Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt" potx
... Controlling the endemic HPAIV H5N1 situation in Egypt is particularly painstaking because of [1] the concentration of the majority of commercial and backyard poultry business in a very small part of the ... viruses Italics indicate use of locked nucleic acid nucleotides The specificity of primers and probes for either or both of the 2.2.1 lineages is indicated with their designation Abdelwhab et ... fragments Reducing the amount of circulating HPAIV H5N1 virus by concerted actions of rapid and specific testing, culling and vaccination of poultry is the key to mitigate the risk of human infections...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... Analytical sensitivity and specificity The analytical sensitivity of our LNA Taqman rRT-PCR for the detection of the HA gene of H5N1 was < 0.5 PFU of virus and 10 copies of ssDNA plasmids No fluorescence ... two of them have been specifically designed for the detection of both clades [19,20] In addition, clinical evaluation has not been performed for most of these assays [15,18-20] Recently, the ... [28] The assay was shown to be sensitive, detecting 10 copies of ssDNA plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The...
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Ứng dụng kỹ thuật RT-PCR phát hiện virus PMWaV-1 gây bệnh héo đỏ đầu lá trên cây dứa Cayenne
... (Pineapple mealybug wilt associated virus-1) CAUSING WILT DISEASE OF CAYENNE PINEAPPLE PLANTS GRADUATION THESIS MAJOR: BIOTECHNOLOGY Professor PhD TRAN THI DUNG BA LUU PHUC LOI HCMC, 09/2006 Student ... 50% 2.4.3 Triệu chứng Theo Sether, D M cs (1998), biểu triệu chứng bệnh thất thường có quan hệ với thời tiết, mật độ rệp sáp hệ gene dứa Hình 2.2 Cây dứa bị bệnh héo đỏ đầu Theo Nguyễn Văn Kế (2002), ... giai đoạn 2.4 Bệnh héo virus 2.4.1 Tác hại Bệnh héo đỏ đầu diện hầu hết vùng trồng dứa giới Theo Sether D.M Hu J.S (2002), bệnh xuất Mỹ, Đài Loan, Singapore, Brazil, Jamaica, Philippines, Venezuela,...
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Analysis, sequencing and in vitro expression of PCR products
... of fragments are generated that all start at the 5′-end of the primer and extend to one of the A positions in the growing chain, and thereby correspond to each T in the template strand When these ... not the touch the side of the tube with the DNA Wash the pellet by adding 100 µl ice-cold 70% ethanol, vortex briefly and centrifuge as before with the tube in the same orientation Aspirate off the ... Comparing the migration rates of the fragments from the different reactions allows the sequence of the DNA to be read starting with the fastest migrating fragments that are closest to the primer...
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Báo cáo Y học: Structural basis for the inhibitory efficacy of efavirenz (DMP-266), MSC194 and PNU142721 towards the HIV-1 RT K103N mutant doc
... by the K103N mutation The electron density for Cc of the K103N mutant structures is well defined but the quality of the map does not allow assessment of the orientation of the amide plane There ... is reduced by the stacking of Od1 in between the plane of the thiourea moiety and the Ca of G190 Hence, the undefined orientation of the N103 amide Od1 and Nd2 atoms may reflect the repulsive forces ... explain the reduced susceptibility of the K103N mutant to NNRTIs The mutation leads to changes in the chemical environment of the NNIBP which affect the interactions to NNRTIs The implication of these...
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Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers doc
... bases at the 3’ end of the miRNA for design of the reverse primer If possible, the last five bases at the 3’ end of the forward primer should include 2-3 A or T residues If possible, the three ... bases at the 3’ end of the forward primer should include 1-2 A or T residues If possible, the two last bases at the 3’ end of the forward primer should include A or T residue If the Tm of the forward ... at the 5’ end of the reverse primer 11 If the Tm of the reverse primer is below 59°C add the following bases at the 5’ end one at a time and calculate the Tm: G, A, C, C, T, G, G, A, C Choose the...
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ỨNG DỤNG KỸ THUẬT RT-PCR PHÁT HIỆN VIRUS PMWaV-1 (Pineapple mealybug wilt associated virus-1) GÂY BỆNH HÉO ĐỎ ĐẦU LÁ TRÊN CÂY DỨA CAYENNE ppt
... (Pineapple mealybug wilt associated virus-1) CAUSING WILT DISEASE OF CAYENNE PINEAPPLE PLANTS GRADUATION THESIS MAJOR: BIOTECHNOLOGY Professor PhD TRAN THI DUNG BA LUU PHUC LOI HCMC, 09/2006 Student ... 50% 2.4.3 Triệu chứng Theo Sether, D M cs (1998), biểu triệu chứng bệnh thất thường có quan hệ với thời tiết, mật độ rệp sáp hệ gene dứa Hình 2.2 Cây dứa bị bệnh héo đỏ đầu Theo Nguyễn Văn Kế (2002), ... giai đoạn 2.4 Bệnh héo virus 2.4.1 Tác hại Bệnh héo đỏ đầu diện hầu hết vùng trồng dứa giới Theo Sether D.M Hu J.S (2002), bệnh xuất Mỹ, Đài Loan, Singapore, Brazil, Jamaica, Philippines, Venezuela,...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... designed within the region of the COG1F-G1NVR and COG2F-G2NVR PCR products Design was based on a annealing temperature of at least 60°C and a minimum of mismatches between the probe and the other genotypes ... all five of the different genotypes of GI stool panel (GI/1, GI/2, GI/3, GI/4 and GI/6) and showed no cross reactivity with any of the GII NoV The GII assay detected all Genotypes of the Genogroup ... confirmed by sequencing of the RT-PCR products The predominance of the GII/4 genotype is consistent with previous sequence analysis of Irish NoV isolates [12,13] and with that of other NoV circulating...
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... uniform throughout the genome The 5'UTR and the terminal 98 nucleotides of the 3'UTR are conserved, but the region of the 3'UTR immediately downstream of the open reading frame and the adjacent U-rich ... variation of the encoded proteins [11,13] The distribution of sequences in a quasispecies clusters around a master sequence, and the "center" of the genetic distribution can be described either as the ... from 5' of the genome Amplicon and are divided into halves named 1x, 1y and 4x, 4y The amplicon boundaries indicate the 5' ends of the innermost amplification primers against the genome of strain...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... designed within the region of the COG1F-G1NVR and COG2F-G2NVR PCR products Design was based on a annealing temperature of at least 60°C and a minimum of mismatches between the probe and the other genotypes ... all five of the different genotypes of GI stool panel (GI/1, GI/2, GI/3, GI/4 and GI/6) and showed no cross reactivity with any of the GII NoV The GII assay detected all Genotypes of the Genogroup ... confirmed by sequencing of the RT-PCR products The predominance of the GII/4 genotype is consistent with previous sequence analysis of Irish NoV isolates [12,13] and with that of other NoV circulating...
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Báo cáo hóa học: " Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses" pdf
... viruses Furthermore, all the serum samples from a panel of 40 healthy individuals analyzed in this study revealed no amplification, thereby establishing the specificity of this assay Evaluation of Multiplex ... importance can be judged from the fact that this assay gives precise information regarding the serotype of the virus Conclusion The development of a suitable effective vaccine and therapeutics for dengue ... Evaluation of multiplex RT-PCR The evaluation of the multiplex RT-PCR assay was carried out with 620 serum samples collected over a period of six years from India Preparation of RNA standard The detection...
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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... uniform throughout the genome The 5'UTR and the terminal 98 nucleotides of the 3'UTR are conserved, but the region of the 3'UTR immediately downstream of the open reading frame and the adjacent U-rich ... variation of the encoded proteins [11,13] The distribution of sequences in a quasispecies clusters around a master sequence, and the "center" of the genetic distribution can be described either as the ... from 5' of the genome Amplicon and are divided into halves named 1x, 1y and 4x, 4y The amplicon boundaries indicate the 5' ends of the innermost amplification primers against the genome of strain...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... the presence of impurities could not be excluded Therefore, reproducible and reliable methods for separation and purification are required for the accurate characterization of these enzymes The ... cultivations of the wild type strain P putida NCIMB 10007 with subsequent multiple purification and separation steps of the involved enzymes We report here the first recombinant Figure Operon of the CAM-plasmid: ... which highlights the advantages of recombinant expression and the fusion of an enzyme to a His-tag Previous studies on the purified protein determined a molecular size of 2,5-DKCMO of 78 kDa by native...
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Luận văn : XÂY DỰNG QUY TRÌNH PHÁT HIỆN VIRUS PMWaV-1 GÂY BỆNH HÉO ĐỎ ĐẦU LÁ (Mealybug wilt) TRÊN CÂY DỨA CAYENNE BẰNG PHƯƠNG PHÁP RT-PCR part 7 doc
... gi|8977608|emb|AL137017.9| Human DNA sequence from clone RP11-120J1 on chromosome Contains the 3' end of a novel gene and a CpG island, complete sequence Length=167585 Score = 44.1 bits (22), ... gi|20334517|gb|AC106726.3| Homo sapiens BAC RP11-450I19 (Ro gi|26108544|gb|AE016762.1| Escherichia coli CFT073 section of gi|146881|gb|J01652.1|ECOMOTAB E coli mocha promoter, motA a gi|48994873|gb|U00096.2| Escherichia ... gi|15487179|emb|AL589706.13| Human DNA sequence from clone RP gi|19068404|emb|AL391737.2|CNS06C8G chromosome I of strain GB gi|61656697|emb|BX088713.8| Zebrafish DNA sequence from clone gi|52627220|gb|AC097087.13|...
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