intricate link between hypoxia and oxidative stress in chronic kidney disease

... oxidative damage, we intend to further investigate the possible link between oxidative stress and mtDNA mutations mtDNA deletions/insertions and base changes mainly involve purine transitions, which ... Results in BioMedicine 2009, 8:10 Background Chronic kidney disease (CKD) is characterized by a state of increased oxidative stress [1] This has been evidenced by changes in oxidative stress biomarkers ... of our findings, we have proposed a possible gateway to further investigate the association between mtDNA mutations and oxidative stress in chronic renal failure References Competing interests...

Ngày tải lên: 11/08/2014, 07:21

... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... (hypobaric hypoxia) and reperfusion damage in brain The increased activities of AS and AL indicate the effective recycling of citrulline to arginine and suggest a functional role to citrulline –NO...

Ngày tải lên: 26/10/2012, 09:07

... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... transients during reversible brain ischemia Exp Brain Res 104, 462–466 188 Silver IA & Erecinska M (1992) Ion homeostasis in rat brain in vivo: intra- and extracellular [Ca2+] and [H+] in the hippocampus ... few minutes later leads to an irreversible and sustained increase in intracellular [Ca2+]c in apical dendrites [151] DCD is invariably demonstrated in every neuronal cell type studied, i.e spinal...

Ngày tải lên: 07/03/2014, 12:20

... amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis that oxidative stress and inflammation represent important but distinct therapeutic ... only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that lipid peroxidation products contribute minimally to brain inflammation in this model, and raise ... animals were divided in two groups (n = 10 each), and randomized to receive placebo, or simultaneously indomethacin (10 mg/liter) in their drinking water, and vitamin E (α-tocopherol) in their diet...

Ngày tải lên: 19/06/2014, 22:20

... ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative stress and angiogenesis is emphasised in SSc by the baseline correlation between ... nifedipine can ameliorate endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1 Endothelium injury is critical in SSc and is suspected ... Friedlander M, Capelliere-Blandin C, NguyenKhoa T, Nguyen AT, Zingraff J, Jungers P, Descamps-Latscha B: Advanced oxidation protein products as a novel marker of oxidative stress in uremia Kidney Int...

Ngày tải lên: 09/08/2014, 01:23

... by local inflammation and changes in the subchondral bone Increasing age, excessive loading or injury, genetic predisposition and obesity are important risk factors for the development and progression ... concentration is identical to and reflects that in the plasma, both under normal conditions and in noninflammatory and inflammatory types of arthritis, excluding those associated with infections [12] To ... were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein assay kit (Sigma Chemical) The samples (25 μg protein) and molecular...

Ngày tải lên: 09/08/2014, 14:21

... to increase radical generation and thereby circumvent the difficulty in inducing oxidative stress by solely increasing pO2 [49] Menadione and paraquot are most frequently used to increase intracellular ... agents that increase intracellular oxidative stress altered transactivation by EBNA1 and Tat in a manner opposite to the effect of hypoxia Differing effects of the oxidizing agents menadione and paraquot ... epitope-tagged EBNA1 and Tat (A) Diagrams of epitope-tagged EBNA1 and Tat EBNA1 is 641 a.a long and binds 20 sites in the EBV FR through its DNA binding domain (DBD) EBNA1's UR1 domain, essential for...

Ngày tải lên: 12/08/2014, 04:20

... subunit is present in mouse brain in cortex, in hippocampal neurons, and in Purkinje cells [73] Neuronal cells respond to toxic insults including ischemia [73], zinc overload [72], and nerve growth ... global inhibition of src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream ... http://www.jneuroinflammation.com/content/3/1/30 kinases Lyn and Fyn as well as the tyrosine kinase Syk [30,32,52] Activation of these signaling cascades are linked to the synthesis and secretion of proinflammatory molecules and cytokines...

Ngày tải lên: 19/06/2014, 22:20

... Angiotensin-converting enzyme inhibitors  When the initiation of ACE inhibitor therapy leads to an increase in serum creatinine levels >30% above baseline  ACE inhibitors should be discontinued, ... reducing disease progression in both the heart and the kidney Arch Intern Med 2000 Mar 13;160(5):685-93 14 Angiotensin-converting enzyme inhibitors  In patients with moderate or severe renal insufficiency, ... anemia to individualized targets * KDIGO 2012 Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease Diuretics Major clinical role in reducing fluid overload in patients...

Ngày tải lên: 14/06/2015, 16:05

... ml / min] = ccreaurine * Vurine t * ccreaserum Ccreaurine: concentration of creatinine in urine [µmol/l] Vurine: Volume of urine [ml] t: time [min] ccreaserum: concentration of creatinine in serum ... content in mice aortas after warfarin diet, UniNx, EC or in healthy aging WT (C57BL/6) and Gas6-/- mice Figure 48: Von Kossa staining of aorta, heart and kidney after uninephrectomy in WT compared ... present in atherosclerosis, ageing, chronic kidney diseases and diabetes and is strongly associated with an increased morbidity and mortality Calcification of arteries occurs at the tunica intima and...

Ngày tải lên: 19/11/2015, 16:48

... orthologs in other plant species, including MPK3 and MPK6 in Arabidopsis (group A MAPKs), SIMK and SAMK in alfalfa, and LeMPK1 ⁄ (Lycopersicon esculentum SA-induced protein kinase) and LeMPK3 ... accumulation in wild-type and transgenic plants in response to abiotic and abiotic stresses (A) Virus infection-induced H2O2 accumulation detected by DAB staining (B) Abiotic stress- induced H2O2 and OÀ ... activity increases in response to mechanical injury and other stress signals, including ABA, JA, and H2O2 [23] Recently, it was shown that maize ZmMPK7 was induced by ABA and H2O2, and that H2O2 may...

Ngày tải lên: 14/03/2014, 23:20

... activity, and the C-terminal domain, containing an iron-binding domain, which is involved in ADH activity The integrity of both domains and that of the iron-binding domain are required for ALDH ... 2010 FEBS 3383 Fermenting enzymes and oxidative stress in Entamoeba E Pineda et al Table Kinetic parameters of EhPFOR at 37 C Figures in parentheses indicate numbers of individual amebal extracts ... buffer and incubated for h on ice, and the remaining EhPFOR activity was determined A control experiment was prepared with a sample diluted in N2-purged buffer, and incubated for h on ice in the...

Ngày tải lên: 15/03/2014, 23:20

... containing PM10 and the other filter containing PM2.5 Preparation and extraction of filters Teflon filters were baked for h at 100oC and transferred into 50 ml conical tube with the particles facing ... Jun-B, and Jun-D) proteins that bind with antioxidant response element (ARE) and regulate expression and induction of NADPH:quinone oxidoreductase and glutathione-S-transferase gene, encoding antioxidant ... PM2.5 induced more MDA formation than PM10 suggesting that PM2.5 induced more oxidative stress than PM10 In the single strand breakage assay using pBR322 DNA, the ratio of changed form II/total...

Ngày tải lên: 07/08/2014, 17:22

... However, after 85 days, the NR activity in the seedlings was lower and decreased with increasing O3 At that time in the season and with increasing oxidative stress, NH3 may have been provided from ... of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This enzyme ensures a high level of GSH regeneration and thus an ... of material from a single leaf was sampled from each ush of each seedling, cut into ì mm pieces and incubated in mL of 100 mM phosphate buer (pH 7.5) containing 40 mM KNO3 and 1.5% 1-propanol...

Ngày tải lên: 08/08/2014, 00:22

... senescence remains unclear Our findings, demonstrating the oxidative stress (ROS) induced telomere erosion and replicative senescence in chondrocytes, suggest the involvement of oxidative stress in both ... 4°C, and then paraffin blocks were prepared using standard histological procedures For nitrotyrosine staining, the sections were deparaffinized and hydrated, and then were immunostained using ... oxidative stress in OA cartilage, as discussed above, we postulated that oxidative stress induces telomere instability and dysfunction in chondrocytes, subsequently resulting in cartilage ageing and...

Ngày tải lên: 09/08/2014, 06:22

... effective in protecting PC12 cells from KA-induced injury in a dose-dependent manner GABA and PETL extract decreased KAinduced Ca2+ and ROS release and lipid peroxidation in PC12 cells and FVB ... release and increase in cell viability caused by the PETL extract and GABA were consistent with the in vivo data (B) 100 Figure DAPI and TUNEL staining of hippocampus form KAstressed mice KA induced ... Discussion The main result of the present study is the finding PETL and GABA protected animals from KA-induced brain injury MDA and apoptosis were significantly reduced in the GABA and PETL-treated...

Ngày tải lên: 10/08/2014, 10:20

... between clinical and molecular parameters Correlations between clinical and molecular parameters (A) Final configuration to Multidimensional Scaling (MDS) (B) Adjusted Bayesian linear model between ... apoptosis in some cells and proliferation in others Reports indicate either exacerbation or amelioration of pathological conditions in the brain during TNF- treatment, including experimentally induced ... with norepinephrine, dopamine and serotonin [32], to initiate their oxidation, which then continues with the production of more ROS, quinones, etc Damage to brain readily releases iron (and copper)...

Ngày tải lên: 11/08/2014, 08:22

... 2006, 7:69 Introduction The most important factor causing chronic obstructive pulmonary disease (COPD) is cigarette smoking which causes increased oxidative and nitrosative stress in this disease ... expression of iNOS (a), MPO (b), 4-HNE (c) and nitrotyrosine (d) in the induced sputum of non-smokers and smokers The expression of iNOS (a), MPO (b), 4-HNE (c) and nitrotyrosine (d) in the induced ... were performed and the mean value was used for analysis Sputum induction and processing A standard procedure of induction was conducted using 4.5% hypertonic saline given at 5-min intervals for...

Ngày tải lên: 12/08/2014, 16:20

... data suggest that in the Doberman pinchers copper accumulates in time and, finally, will have its negative effect on copper metabolism and induce oxidative stress Oxidative stress has been ascribed ... with copper incorporated during biosynthesis Metallothionein 1A (MT1A) is a small intracellular protein capable of chelating several metal ions, including copper It contains many cysteine residues, ... specialized and complex systems for maintaining intracellular copper concentrations [3] If this balance is disturbed, excess copper can induce oxidative stress that could lead to chronic inflammation...

Ngày tải lên: 13/08/2014, 13:20