intracellular redox state modifies the action of cell signaling pathways
Báo cáo sinh học: " The involvement of survival signaling pathways in rubella-virus induced apoptosis" ppt
Ngày tải lên :
18/06/2014, 22:20
... increase in the speed and magnitude of RV-induced apoptosis To evaluate the role of PI3K-dependent signaling during RV infection, the effects of PI3K inhibitor LY294002 on the development of RV-induced ... RV-infected cells and RV in the presence of U0126 (Fig 3C), although the presence of the drug also appeared to result in smearing of high molecular weight DNA, characteristic of necrosis [26,27] The ... tissues would be necessary to confirm the theory that the pathogenesis of the disease is related to perturbation of the cell cycle However as CRS is now rare in the UK and work with fetal tissues...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
Ngày tải lên :
16/02/2014, 09:20
... promoters, mutation of the CHR leads to a more pronounced deregulation than the alteration of the CDE [40] Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein ... for the cell cycle-dependent expression The sequence upstream of the CHR does not match any of the published CDEs However, alteration of a putative CDE, which is more distant from the CHR than the ... Myb site in activating the Cdc2 promoter Binding of the activating E2Fs during the cell cycle to the promoter alternates with binding of E2F4 in quiescent cells Mutation of the distal E2F site allows...
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Báo cáo khoa học: Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells pptx
Ngày tải lên :
23/03/2014, 15:21
... immunostaining using the antibodies indicated The scale bar represents 20 lm of intoxication Neither the human leukemic cell line HSB-2 nor the Burkitt lymphoma Ramo cells showed any potentiation of cytotoxicity ... polypeptides all showed the expected electrophoretic mobility, those of the mutants being decreased compared with the wild-type chimera At the end of the 24 h chase period, most of the ATFSAPREDLK polypeptides ... toxicity We therefore concluded that the Golgi complex is not a major intracellular compartment for productive trafficking of saporin When we investigated the intracellular route of a human prourokinase–saporin...
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Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc
Ngày tải lên :
23/03/2014, 17:21
... description of the experimental conditions and of the calibration method of fluorescence signal vs membrane potential is given in previous reports [33,34] Determination of intracellular pH The intracellular ... taken advantage of the fact that bacteria of the class Mollicutes are devoid of an outer membrane and of a cell wall, to avoid possible interferences of these structures in the interactions between ... MDC100 are the minimal concentrations (lM) required for the deformation of 50% and 100% of the cells, respectively, upon of action No stands for no observed effect DDY is the percentage of membrane...
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Báo cáo sinh học: "The nature of cell-cycle checkpoints: facts and fallacies" docx
Ngày tải lên :
06/08/2014, 19:21
... (phase) of the cell cycle without considering the original meaning of the term Just as there is no such thing as a G2/M cell or a G2/M phase, there is no such thing as a G2/M checkpoint Rather, there ... for the different phases of the cell cycle and determine the targets of the kinases Exit from each phase of the cell cycle occurs on degradation of the bound cyclin The CDK-cyclin complex that is ... result in the production of an unattached kinetochore detected by the checkpoint See text for details pathways that respond to everything from the status of p53 or p38 to the integrity of the chromosomes...
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Báo cáo toán học: "The Action of the Symmetric Group on a Generalized Partition Semilattice" potx
Ngày tải lên :
07/08/2014, 06:20
... was first stated in [3]) A version of it states that if the homology of a poset Q is concentrated in a single dimension r, then the character of the action of a group G o on the homology of Q is ... Hi,j,r for some r, then i and j are in the same block of π(Y ) Therefore, each Ci is a block of π(hσ ) Then dim(hσ ) is the number of blocks of π(hσ ) and the number of cycles of σ For example, ... P = Πn,k and consider the action of the symmetric group Sn on P , permuting the coordinates of the elements We consider the subposet P σ , which consists of the elements of P that are fixed by...
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Báo cáo y học: "Birth order modifies the effect of IL13 gene polymorphisms on serum IgE at age 10 and skin prick test at ages 4, 10 and 18: a prospective birth cohort study" pps
Ngày tải lên :
08/08/2014, 21:20
... Wight, U.K The Isle of Wight is an island (13 × 23 miles) just off the South Coast of England with a resident population of 130,000 The population is stable to the extent that the majority of participants ... at the 3’ UTR/exon position of the gene Each of these SNPs had a minor allele frequency ≥ 19% Using the Haploview program [26], three of the five single nucleotide polymorphisms (SNPs) of the ... more than the product of the individual effects Following the pattern of previous publications [27,37,38], we estimated the effect of IL13 polymorphisms using the dominant model, i.e the heterozygous...
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Báo cáo y học: "Alterations in peripheral blood memory B cells in patients with active rheumatoid arthritis are dependent on the action of tumour necrosis factor" potx
Ngày tải lên :
09/08/2014, 14:21
... currently unknown whether this approach to treatment is successful because of the production of early plasma cells due to the loss of rheumatoid factor or because of other functions of B cells Functionally ... cells/μl) These differences could not be the result of B cell lymphopenia, because the absolute number of the total B cell pool in the long-standing RA patients was comparable to the healthy Page of ... [39]) from the cell surface of pre-switch memory B cells However, the negligible number of somatic mutations in the VH genes of the IgD+CD27- subset and the comparable serological levels of soluble...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
Ngày tải lên :
11/08/2014, 00:22
... natural barrier of the genital epithelium, therefore inactivating the ability of the HIV virus to reach the target cells that reside below Thus, when the HIV virus crosses the genital epithelium, it ... upper chamber of the Transwell to evaluate inhibition of HIV-1 cell- associated transmission Target cells (MT- 2) were added to the lower chambers In the case of the MT-2 cells, half of the media ... of subepithelial cells, suggesting that genital epithelial cells are not susceptible to HIV infection and play no part in the transfer of infectious virus across the epithelium As a result, these...
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báo cáo khoa học: " Increased hepatic oxidative metabolism distinguishes the action of Peroxisome proliferator-activated receptor d from Peroxisome proliferatoractivated receptor g in the ob/ob mouse" ppt
Ngày tải lên :
11/08/2014, 12:20
... activated The exact balance between the concentrations of these pathways presumably arises from the balance between increased βoxidation and the actual activity of the synthetic pathway across the ... analysis of the changes in intact lipid metabolism induced by stimulation of PPARd and PPARg (a) PLS-DA scores plot showing the clustering of the UPLC-MS chromatograms from the organic fraction of ... Multivariate analysis of some of the key metabolic changes induced by PPARd stimulation (a) PLS-DA scores plot showing the clustering of the GC-MS chromatograms from the organic fraction of liver tissue...
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Báo cáo y học: " Classic swine fever virus NS2 protein leads to the induction of cell cycle arrest at S-phase and endoplasmic reticulum stress" potx
Ngày tải lên :
12/08/2014, 04:21
... on the subcellular localization of CSFV NS2 protein and its effects on cell growth and cell cycle progression The swine endothelial vascular cell is one of the CSFV target cells, vascular endothelial ... vein endothelial cell line (SUVEC) established in our lab previously [13] and increased the proportion of the cells in the S-phase with a concomitant decrease in the proportion of cells in the G0/G1 ... cycle arrest at S-phase To determine whether the growth inhibition of CSFV NS2-expressing cells was due to the arrest of the cell cycle at a certain phase(s) of cell division, flow cytometric analysis...
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Báo cáo khoa học: " Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities" doc
Ngày tải lên :
12/08/2014, 04:22
... by PVP-I of influenza A virus growth in MDCK cells We first determined the cytotoxicity of PVP-I against MDCK cells employed as host cells of influenza viruses in this study by using a cell counting ... of the virus titer by more than orders of magnitude in Madin-Darby canine http://www.virologyj.com/content/6/1/124 kidney (MDCK) cells [28] However, the target sites and mechanisms of PVP-I action ... equivalent to a multiplicity of infection (MOI) of 0.025 PFU /cell, in the absence of an inhibitor [41]) was preincubated with an equal volume of serial dilutions of an inhibitor (I) or serum-free...
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Báo cáo y học: " On the steps of cell-to-cell HIV transmission between CD4 T cells" pptx
Ngày tải lên :
12/08/2014, 23:22
... transfer of membrane lipids from MOLT infected cells to target cells Membrane transfer to single cells may be the consequence of either hemifusion events between cells [22], or the transfer of viral ... the VS On the other hand, the transfer of membranes from NL4-3 and BaL infected MOLT cells to the MT-4 cell line is lower than that observed in primary cells Notably, only the transfer of membranes ... widely observed that the formation of the synaptic structure is dependent on Env binding to CD4 [9,10,15] However, the role of the co-receptor during the early events of the VS is still under...
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Báo cáo y học: "Prior mucosal exposure to heterologous cells alters the pathogenesis of cell-associated mucosal feline immunodeficiency virus challenge" docx
Ngày tải lên :
13/08/2014, 01:20
... copies available at the time of this study, the data suggests that a reduction of the number of T-regulatory cells within the environment was associated with the development of an effector immune ... understood The role of cell exposure is particularly relevant when considering mucosal transmission Not only is mucosa the major route of cell- free and cell- associated HIV-1 transmission [22-25], the ... with the standards of the American Association of Accreditation of Laboratory Animal Care and The Ohio State University Institutional Animal Care and Use Committee Peripheral blood mononuclear cells...
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Báo cáo y học: " A statistical approach to estimating the strength of cell-cell interactions under the differential adhesion hypothesis" pptx
Ngày tải lên :
13/08/2014, 16:21
... estimating the nature of these interactions may play a key role for an early detection of cancer In addition, the invasive nature of some tumors is directly linked to the modification of the strength of ... as it determines the relative contribution of cell- cell interactions in the energy It may reflect the general state of a tissue, and its inference is relevant to applications of the model to experimental ... (i, j) are the pixel spatial coordinates, σij represents the cell to which the pixel (i, j) belongs, τ(σij) denotes the type of the cell σij, and the function J characterizes the interaction intensity...
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Báo cáo y học: "Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury" potx
Ngày tải lên :
13/08/2014, 21:21
... importantly, the net effect of shear stress depended on the inflammatory state of the interacting cell populations Firm adhesion of non-activated PMN to maximally activated HUVEC showed the smallest ... leukocyte accumulation throughout the course of the syndrome Regarding the role of platelets, our observations add a further piece to the puzzle of platelet-neutrophil interactions during severe inflammation ... within the metal case Preliminary experiments showed that temperature of the metal block equaled with temperature of the perfusate within few seconds For microscopy of the adhesion assay, the whole...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
Ngày tải lên :
13/08/2014, 23:20
... newborn cell of size 1.0 During the first interval of growth the cell would make 0.1 units of cell mass so that at the end of the interval the mass is 1.1 units During the next interval of growth ... weighted sum of these growth patterns As the cytoplasm is by far the major component, the other parts of the cell not contribute measurably to the growth pattern of the whole cell It is of interest ... due to the initial cell age distribution of the cells at the time they were filtered The decrease in the dashed line is placed at the end of the first division cycle as indicated by the cell elution...
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The action of bee venom cobra venom and lysolecithin on the adrenal medulla
Ngày tải lên :
24/10/2014, 07:17
... fall due to the depressor action of the venom In the experiment of Fig the injection of jig of venom (at B) after a latency of about caused a small rise of pressure lasting a few minutes The subsequent ... be the decisive factor and the proteolytic action of a more secondary nature for the symptomatology For instance, the action on the perfused guinea-pig's lung of trypsin and of the venom of Crotalus ... upon the results obtained by the action of lysolecithin on suspended cellular material of the adrenals, upon the relative insensitivity of the cat's adrenals to histamine, and upon the absence of...
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The action of snake in venoms on surfaces film
Ngày tải lên :
24/10/2014, 07:17
... (i) The PH of the underlying solution (ii) The surface concentration of the lecithin film (iii) The venom concentration These effects were examined in detail (i) The effect of PH The action of ... varying amounts of protein in the solution and the possible adsorption of the venom on the glass vessel as well as at the surface of the film It must be remembered that the values of the venom concentration ... of the lecithin will alter the spacing of the essential points of attachment, and at the higher compressions the double bonds will be removed from the aqueous surface, as observed in the case of...
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