intracellular delivery of proteins with ph responsive polymers

... for the cofactor of the immobilized proteins is only possible with nanostructured Ag for direct excitation of surface plasmons down to 400 nm With the recent discovery of the coupling of surface ... spectroscopy: since the electronic transitions of most of the protein cofactors (e.g haem) of proteins are at shorter wavelengths, the combination of molecular RR and the surface-enhanced Raman ... excitation lines in resonance with an electronic transition of the cofactor of the protein, to selectively enhance those Raman bands that originate from the chromophoric part of the macromolecule [resonance...

Ngày tải lên: 22/03/2014, 16:20

... Conditioned medium of nontransfected Cos7 cell (a), ng of BNC from the conditioned medium (b), 100 ng of EGFP (c) and a mixture of ng of BNC with 100 ng of EGFP (d), and ng of L(n45)-FLAG-EGFP ... particle Topology analysis of the C-terminus of L fusion protein Consisting of 56 amino acids, the primary structure of the C-terminus of the S region is rich in hydrophobic residues Therefore, ... Bio-nanocapsules for protein delivery pattern of topology might clarify the results of previous studies of the topology of the HBsAg protein It is suggested that the C-terminus of the envelope protein...

Ngày tải lên: 23/03/2014, 15:20

... http://www.nanoscalereslett.com/content/6/1/608 Page of (a) (b) (c) Figure TEM and confocal microscopy images and flow cytometry analysis of HeLa cells (a) TEM photograph of HeLa cells treated with f-HNT-ASODN-FAM complexes ... washed three times with PBS, dehydrated through a series Page of of alcohol concentrations (30%, 50%, 70%, 90%, 100%), embedded in Epon, and sliced to a thickness of 70 nm Images of the sliced images ... density of × 106 cells in a 60-mm tissue culture dish and grown overnight The cells were incubated with the f-HNT-ASODN complexes for h, and then the cells were washed thoroughly with chilled phosphate-buffered...

Ngày tải lên: 20/06/2014, 23:20

... [27] It has been shown that up to 33% of eukaryotic proteins contain long disordered regions, compared with 2% of archeal proteins [25] The characterization of RTN4 as an intrinsically unstructured/disordered ... associates with calreticulin-negative regions of the ER and co-immunoprecipitates with the SNARE proteins syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2 [39] Overexpression of a fragment of RTN1C ... Volume 8, Issue 12, Article 234 Interacting proteins Phospholipid bilayers Yip3p Yop-1/DP1 TBC1D20 Rab5 Intracellular reticulon proteins RME-1 AP-2 SNARE proteins: syntaxin syntaxin syntaxin 13 VAMP2...

Ngày tải lên: 14/08/2014, 08:20

... e-value cutoff BLAST e-value Figure Representation of POFs in 10 different eukaryotic genomes Representation of POFs in 10 different eukaryotic genomes POFs represent 18% to 38% of the proteins ... human-specific proteins are POFs or PDFs we performed a BLAST search (E-value cutoff of 10-6) of all published sequences against the human proteome Consistent with POFs representing the majority of species-specific ... is an analysis of the origins and functions of genes encoding proteins that currently lack defined motifs or domains (proteins with obscure features (POFs)) [7,8] Expression profiling studies...

Ngày tải lên: 14/08/2014, 16:21

... noitaulave evitatitnauq rof desu saw tnemirepxe nA tnemirepxe na gnirud ytivitisnes ni ssol elbaredisnoc a dewohs sedortcele +2gM tsom ,tnemeerga doog ni yllausu erew sedortcele +aN rof sevruc noitarbilac ... eht esaercni ot deleveb neht dna ,sedortceleorcim evitceles-+2gM eht rof 2lCgM Mm 001 dna sedortceleorcim evitceles-+aN eht rof lCaN Mm 001 htiw dellifkcab erew sedortceleorcim dezinalis-enalisorolhclytubirt ... esuac dna ecrof gnivird sti esaercni dluow noitacifidica ralullecartxe ,]81[ muilehtipe eht ni sa ammelocras caidrac eht ni tneserp erew egnahcxe +H/+2gM fI xulfni eht ni tnemevlovni rof etadidnac...

Ngày tải lên: 07/08/2014, 18:21

... and how these may affect delivery of services within the PCO Understanding of service redesign and care pathways Knowledge of local educational providers Indicators of sexual health risk Skills ... should form part of the GPwSI annual appraisal and Evidence of delivering a sexual health service of quality within his/her general practice Sexual Health Examples of different evidence of competencies ... Good understanding of roles, responsibility and structure of PCOs and how to influence them to bring about improvement in delivery of sexual health services Understanding of primary care structures...

Ngày tải lên: 14/02/2014, 21:20

... correlated with the amount of GmPDIL-3a mRNA, although the amount of GmPDIL-3b mRNA was not consistent with the amount of GmPDIL-3b protein expression (Fig 5B) Expression of many ER-resident proteins ... °C for 16 h For crosslinking of proteins, slices of cotyledons were homogenized with 20 strokes of a Dounce homogenizer at °C in mL of buffer containing 20 mm Hepes (pH 7.2), 150 mm NaCl, 1% protease ... mammalian PDI family proteins function together with other PDI family proteins or with molecular chaperones to effectively fold nascent proteins [20–23], we next tested the ability of GmPDIL-3a and...

Ngày tải lên: 18/02/2014, 11:20

... [precursor] Triosephosphate isomerase Triosephosphate isomerase Triosephosphate isomerase Ubiquinol-cytochrome c reductase iron-sulfur subunit, mitochondrial [precursor] ATP synthase alpha chain, mitochondrial ... interactions It is an intracellular and extracellular lectin, which interacts with intracellular glycoproteins, cell surface proteins and extracellular matrix proteins Overexpression of galectin-3 in ... Examination of differentially expressed proteins was performed by image analysis software (PDQuest 7.3) (Bio-Rad Laboratories) as described [65] Determination of glutamine labelling of proteins of Caco-2...

Ngày tải lên: 20/02/2014, 01:20

... the case of the interaction of DYNLL1 with the phosphoproteins of RV, Mokola virus and ebolavirus On the other hand, a conundrum therefore exists in that certain envelope or capsid viral proteins ... polymerase activity than in the intracellular transport of the virus [74] This is in agreement with the nuclear staining of the P phosphoprotein where it co-localizes with promyelocytic leukaemia ... disassembly (b) This is in agreement with the modeled solution structure of the complex of DYNLL1 with p54 of ASFV [26] (B) Three hypotheses for the association of viral proteins to the dynein molecular...

Ngày tải lên: 14/03/2014, 22:20

... levels of FLAPP by a mechanism independent of direct proteolysis Analysis with C8 also revealed a series of low molecular mass species of sizes consistent with CTFs (Fig 4C) Two CTFs of approximately ... nuclear translocation of the intracellular C-terminal domain (ICD) of the APP family of proteins Biochemistry 42, 6664–6673 Cao X & Sudhof TC (2001) A transcriptionally [correction of transcriptively] ... from mL of homogenate was resuspended in 100 lL of assay buffer (150 mm sodium citrate, pH 6.8) either containing or devoid of a cocktail of protease inhibitors (5 mm EDTA, mm EGTA, mm 1,10-phenanthroline,...

Ngày tải lên: 15/03/2014, 10:20

... glands of amphibian skin constitute one of 5648 the richest [14–16] Studies on the mode of action of amphibian antimicrobial peptides have mainly addressed their interaction with phospholipid ... permeation/proteome buffer (pH 7.4) and when bound to phosphatidylethanolamine (PE) ⁄ phosphatidylglycerol (PG) vesicles of composition : (w ⁄ w), which is typical of the E coli inner membrane ... wheel diagram of Esc(1–18) in a perfect a-helical conformation (Fig 1B) shows amphipathicity of the peptide, with hydrophobic and hydrophilic residues segregating on opposite sides of the molecule...

Ngày tải lên: 16/03/2014, 00:20

... perivasculitis of leptomeninges (arrows) and cortical gray matter (A) The cellular infiltrate was mainly composed of CD-3-positive T-lymphocytes (B) and CD-68-positive macrophages (C) with minimal ... steroid was tapered at a rate of mg/week, and he was discharged on a regimen of methylprednisolone 30 mg/day Discussion CAA is defined by the deposition of amyloid proteins within leptomeningeal and ... of microbleeds are mentioned but the proportion is not mentioned) NA perivasculitis multiple NA (+) vasculitis cyclophosphamide alive relapse (+) 62.3* M encephalopathy headache F3 seizure aphasia...

Ngày tải lên: 19/06/2014, 22:20

... relatively low pH of this compartment Chan and Warwicker [5] show that this correlation of the pH of maximal stability with intracellular pH is only evident when the properties of many proteins within ... between the pH optimum of stability of monomeric proteins and of their complexes [10] This suggests that pH- dependent proper­ ties of the monomers and of the complexes coevolved at the same pH The ... thermophilic proteins Proteins from thermophilic organisms usually have a higher number of charges and ion pairs than their mesophilic homologs [7] An analysis of the number and types of charges...

Ngày tải lên: 06/08/2014, 19:21

... counterstained with hematoxylin Statistical analysis Statistical analysis was performed with GraphPad software (GraphPad Sofware Inc., San Diego, CA, USA) For microarray analysis a t test was used with ... OA could be correlated with markers of systemic and in part with markers of local inflammation as well as with disease duration A relation of BMP suppression to therapy with steroids and disease-modifying ... buffer (50% formamide, 80 µl of 50 × Denhardt's solution, 1.6 ml of 20 × SSC, 200 µl of herring sperm, 100 µl of carrier RNA) without the riboprobe Hybridization with digoxigeninlabeled riboprobes...

Ngày tải lên: 09/08/2014, 07:20

... Neuwirth et al One of the pathologic hallmarks is inflammation, with consequent hypertrophy of the subacromial bursa [3,4] Hypertrophy of the bursa is regarded as one of the causes of the chondroid ... References without C2C12 cells BMP-2 and BMP-7 co-localization with AP in bursa tissue with and without C2C12 cells Indirect immunofluorescence staining of the topical distribution of (a) bone morphogenetic ... cartilage A comparison of mRNA profiles for select genes from bursa tissue, tendon and cartilage (a) Gel-electrophoretic profile of reverse transcription (RT)-PCR from RNA of a subacromial bursa...

Ngày tải lên: 09/08/2014, 08:22

... (pCI-Revincreasing amounts of Rev either HIV-1 or withRRE Effect of4 M10) on titer of vector stocks produced plasmid Effect of increasing amounts of Rev M10-encoding plasmid (pCI-Rev M10) on titer of vector stocks ... by the author with technical assistance of Ms Margo Camel in some of the experiments Flow cytometry profiles of Jurkat T-cells challenged with pNL4 R-EHSA+ virus Each population of Jurkat T-cells ... validity of this observation in the context of gene delivery systems, different amounts (0 μg to 1.0 μg) of an M10 expression construct, pCI-Rev M10, were added during production of vector stock with...

Ngày tải lên: 10/08/2014, 05:21

... community Stories collected from 19 of the 23 people with stroke will be used to inform future stroke participants of the process of getting out of the house with therapy support (Barnsley, McCluskey ... measured was delivery of multiple outdoor journey sessions to people with stroke, consistent with a national guideline recommendation A secondary aim was to evaluate the impact of practice change ... 34% change) Initially, 36% of people with stroke whose records were audited received six or more Page of 10 Table Proportion of medical records audited where people with stroke received six or...

Ngày tải lên: 10/08/2014, 10:23

... Hashim OH: Profiling of serum and tissue high abundance acute-phase proteins of patients with epithelial and germ line ovarian carcinoma Proteome Sci 2008, 6:20 Doustjalali SR, Yusof R, Yip CH, ... Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies Electrophoresis 2008, ... serum protein profiles of negative control women and patients in different stages of EOCa Typical 2-DE serum protein profiles of negative control women and patients in different stages of EOCa Unfractionated...

Ngày tải lên: 10/08/2014, 22:20

... eosinophils 1%, basophils 7%, bands 16%, polymorphs 26%, lymphocytes 2% and platelets 917 × 10 9/L Leukocyte alkaline phosphatase was 11 Bone marrow examination was consistent with chronic phase ... cells with 46, XX Repeat FISH analysis of this sample confirmed 200/200 metaphase cells to be Ph- negative After receiving two courses of induction chemotherapy consisting of CMOP regimen (cyclophosphamide, ... leukaemia without Philadelphia chromosome developing after interferon therapy for chronic myelocytic leukaemia with Philadelphia chromosome Br J Haematol 1995, 90:951-953 Wang et al Journal of Hematology...

Ngày tải lên: 10/08/2014, 22:21