internal standardization isolation and derivatization
Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
Ngày tải lên :
05/09/2013, 09:08
... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
Ngày tải lên :
18/02/2014, 08:20
... wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf produced a predominant band with an apparent ... purified proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
Ngày tải lên :
18/02/2014, 16:20
... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS Half maximal inhibitory concentration (IC50) and median lethal dose (LD50) values from HeLa cells and mice divided ... numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... these cells Mesenteric BECs and LECs (Fig 5A,B) were also obtained by the same strategy as illustrated in Figs and 4, and were treated with VEGF-A or VEGF-C (Fig 5C) MAPK and Akt phosphorylation ... induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolation and characterization of BECs and LECs We next ... introduced by lipofection and maintained for at least days after transfection as assessed by GFP expression (Fig 4E) These Fig Isolation and characterization of uterine BECs and LECs expressing tsA58T...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
Ngày tải lên :
21/02/2014, 00:20
... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and averaged ... 12768–12785 Pulver, S.C., Froland, W.A., Lipscomb, J.D & Solomon, E.I (1997) Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
Ngày tải lên :
21/02/2014, 00:20
... column, equilibrated and eluted in 0.1 M Tris/HCl, pH 7.6 and several common protein–protein dissociation salts (1 M sodium chloride, M urea and M guanidinium chloride) and ionic detergents (CHAPS, ... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for...
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Tài liệu 2009 Internal Audit Capabilities and Needs Survey pot
Ngày tải lên :
26/02/2014, 04:20
... understanding of IFRS and where it differs from U.S GAAP, but also a specific understanding of how these new standards will impact policies, procedures, systems (and systems interfaces) and data ... control and governance processes is adequate and fully functioning.” Internal Audit Capabilities and Needs Survey 13 Trends by Company Size and Industry Responses from large, midsized and small ... helping management and the board understand, assess, mitigate and manage the organization’s risk through activities detailed in the internal audit plan It is incumbent upon CAEs and the internal audit...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
Ngày tải lên :
07/03/2014, 21:20
... or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents systems, the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ... (2H, m, Hb-2 and Hb-6 ) and 2.00 (2H, m, Hb-3 and Hb-5 ) The 13C NMR spectrum of compound displayed signals at d: 22.8 (C-3 and C-5), 27.5 (C-7), 28.8 (C-9 and C-10), 31.5 (C-2 and C-6), 32.9 ... not be separated from each other by CCC Since K2 and K4 were close to K3 and K5, respectively, curcumol (2) and germacrone (3), and curzerene (4) and 1,8-cineole (5) were hardly separated from...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
Ngày tải lên :
08/03/2014, 10:20
... described in the Materials and methods phenolic compounds and alginates, etc and hence preserved the enzymatic activity of lamjapin But the efficiency of extraction was still low and the proteins could ... Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate J Biochem Biophys Methods 11, 13–20 Neuhoff, V., Stamm R & Eibl, H (1985) Clear background and highly ... proteins and also to the low efficiency of extraction Physical properties of lamjapin Lamjapin obtained from the Mono Q column migrated as a single band characterized by both 12% SDS/PAGE and isoelectric...
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Internal Control Management and Evaluation Tool docx
Ngày tải lên :
08/03/2014, 14:20
... On the other hand, this tool discusses internal control from a broader, overall entity perspective based on the internal control standards and focusing on management’s operational and program objectives ... employees are subject to standards and principles of ethical conduct in accordance with 5CFR2635 and Executive Orders 12674 and 12731 Page GAO-01-1008G – Internal Control Management and Evaluation Tool ... congressional and GAO staff to discuss major issues affecting operations, internal control, performance, and other major agency activities and programs Page 21 GAO-01-1008G – Internal Control Management and...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
Ngày tải lên :
08/03/2014, 16:20
... 95% (w/v) ammonium sulfate and incubated for 20 at °C The suspension was centrifuged at 15 000 g for 30 and the pellet was resuspended in 10 mL 30 mM Tris/HCl, pH 7.9, and dialyzed against L 30 ... NcoI and BamHI, and cloned into the pET-8c vector Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas was a gift from  Á M Goldschmidt-Clermont (Universite de Geneve, Switzerland) ... mM Na2EDTA, 7% SDS, and 1% BSA After puri®cation of positive clones, k DNA was extracted and puri®ed as described in [13] cDNA inserts were excised by a digestion with EcoRI and cloned into the...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
Ngày tải lên :
08/03/2014, 23:20
... c-terpinene and is therefore designated ClcTS (Fig 3B), C62 and M34 both produced limonene and are designated Cl(+)LIMS1 and Cl(+)LIMS2, respectively (Fig 3C,E) and D85 produced b-pinene and is designated ... C62 (D) D85 and (E) M34 B93 mainly produces c-terpinene, C62 and M34 produce limonene and D85 mainly produces b-pinene Peak identities were confirmed using standards, whose mass spectra and retention ... rich in serines and threonines and low in acidic and basic amino acids and about 45–70 amino acids long Usually they show only little homology The predictions using PREDOTAR and TARGETP indicate...
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Internal audit independence and objectivity: a review of current literature and opportunities for future research ppt
Ngày tải lên :
15/03/2014, 23:20
... attribute standards and associated advisory statements relating to independence and objectivity Standard 1100 states that “the internal audit activity should be independent, and internal auditors ... composition and the committee's interaction with internal audit Goodwin and Yeo (2001) Survey 65 Singaporean Internal Auditors Goodwin (2003) Survey 120 Internal auditors from Australia and New Zealand ... and consultant can create bias and hence cause problems for internal audit objectivity Both Brody and Lowe (2000) and Ahlawat and Lowe (2004) examined whether internal auditors can remain objective...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
Ngày tải lên :
16/03/2014, 18:20
... higher d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the ... bands at about 42, 43 and 44 kDa were detected, while no bands were visible in chloroplast extracts (Fig 3) One could assume that in mitochondria the unprocessed protein, the mature protein and ... Qiagen) and 110 lL medium IV (4 g PEG 4000, mL H2O, 2.5 mL 0.8 m mannitol, mL m CaCl2) and incubated for 30 at 23 °C To remove the PEG mL medium II was added, centrifuged for at 95 g and °C, and...
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ADVANCES IN INTERNAL COMBUSTION ENGINES AND FUEL TECHNOLOGIES pptx
Ngày tải lên :
17/03/2014, 02:20
... figures and 6; those for the 10 to 90% MFB duration (RBA) are given in figures and The burn angles are plotted for three engine speeds and two levels of IMEP and spark advance Both FDA and RBA ... all forms and modes of combustion, including compression and spark ignition, direct and indirect injection and homogeneous charge compression ignition combustion, with a range of liquid and gaseous ... 2.06xb0.77), originally identified by Rhodes and Keck [58] and by 31 32 Advances in Internal Combustion Engines and Fuel Technologies Metghalchi and Keck [59] to represent the detrimental influence...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
Ngày tải lên :
17/03/2014, 10:20
... respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... library was then designed with randomized CDR3 loops, and displayed successfully on the surface of fd bacteriophages and panned for specific binding molecules [10] Third, and most recently we isolated ... CDR1 and CDR3 loops (Type2 VNAR) [5,11] Camelid VHHs typically possess disulphide linkages either between the CDR1 and )3, or CDR2 and )3 loops [13,14] Despite these similarities, the camel and...
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Internal Control Management and Evaluation Tool pdf
Ngày tải lên :
23/03/2014, 03:20
... On the other hand, this tool discusses internal control from a broader, overall entity perspective based on the internal control standards and focusing on management’s operational and program objectives ... employees are subject to standards and principles of ethical conduct in accordance with 5CFR2635 and Executive Orders 12674 and 12731 Page GAO-01-1008G – Internal Control Management and Evaluation Tool ... congressional and GAO staff to discuss major issues affecting operations, internal control, performance, and other major agency activities and programs Page 21 GAO-01-1008G – Internal Control Management and...
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Internal Service Systems and Cultural Differences: An Exploratory Study of Switzerland, Australia, and Singapore pot
Ngày tải lên :
23/03/2014, 04:21
... relationship between internal service culture, internal service quality, and internal service systems by focusing on the internal service gaps and the impact Internal Service Systems and Cultural Differences: ... an internal service system 2.1.2 Internal service systems An internal service system should integrate national, business and organisational culture, internal and external service culture, and internal ... Switzerland, Australia, and Singapore of dimensions of national culture and internal services The research focuses on internal service cultures in multinational companies in Switzerland, Australia and...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
Ngày tải lên :
23/03/2014, 07:20
... re-testing and validating the effects on cell growth by transforming W303.1C and plating the cells at 23 °C, 30 °C and 37 °C A cold-sensitive growth phenotype (where toxin is active and interferes ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... GL and KGM) GL is supported by the University Hospitals of Coventry and Warwickshire NHS Trust We are grateful for access and user support at the synchrotron facilities at ESRF, Grenoble and...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
Ngày tải lên :
23/03/2014, 13:20
... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS chloroplasts were used as ... blue-native (BN) ⁄ PAGE and (2) colorlessnative (CN) ⁄ PAGE and BN ⁄ PAGE, we separated the Ndh complex from both MS and BS chloroplasts and determined its monomeric and dimeric state We also...
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