interleukins interferons and members of the tumor necrosis factor family

... quantitative RT-PCR and participated in the study design and drafting of the manuscript JEG performed statistical analysis and participated in the study design and drafting of the manuscript FL and JS participated ... participated in the study design and drafting of the manuscript TL and NB performed immunocytochemistry experiments CL performed the biopsies of minor salivary glands XM conceived of the study and its ... property of epithelial cells is enhanced in patients with pSS and confirms the importance of resident cells of target organs in inducing or perpetuating autoimmunity and the pivotal role of epithelial...

Ngày tải lên: 09/08/2014, 07:20

... calculations take into account the way the patients distribute along the groups, namely the weight of each group in the sample The predictive probability of the group as a function of the SvdH score (Figure ... activity of -308G and -308A alleles [22,23] Nevertheless, more recent data suggest a direct functional effect of the -308 SNP by modifying the binding of transcription factors [24] On the other hand, ... in cell line types, the length of the promoter sequence, and the presence/absence of the 3' untranslated region [30] Regardless of whether there is a direct functional effect of this SNP, some...

Ngày tải lên: 09/08/2014, 10:20

... because of an unsatisfactory response Six percent of patients (24 of 376) without prior anti-TNF therapy and 3% of patients (two of 66) with prior antiTNF therapy withdrew from the study because of ... During the complete study, 1% of patients with AS (10 of 924) without prior anti-TNF therapy and 3% of patients with AS (11 of 326) with prior anti-TNF therapy withdrew because of an unsatisfactory ... The RHAPSODY and STEREO studies were conducted in accordance with the principles of the Declaration of Helsinki, and the protocols were approved by the institutional review boards of the participating...

Ngày tải lên: 12/08/2014, 14:22

... p38 MAPK, NFB, and TNF receptor depicted at the center of Figure 2a A cluster of the Toll-like receptor (TLR) family (that is, TLR1, TLR2, TLR4, and TLR5) and another cluster of Fc receptors ... genes of the immune response category were further investigated by utilizing network-based analysis A cluster of the TLR family (that is, TLR1, TLR2, TLR4, and TLR5) and another cluster of FcRs ... were used as the background The median and standard deviation of background levels were calculated Genes whose intensities were less than the median plus two standard deviations of the background...

Ngày tải lên: 09/08/2014, 01:22

... p38 MAPK, NFB, and TNF receptor depicted at the center of Figure 2a A cluster of the Toll-like receptor (TLR) family (that is, TLR1, TLR2, TLR4, and TLR5) and another cluster of Fc receptors ... genes of the immune response category were further investigated by utilizing network-based analysis A cluster of the TLR family (that is, TLR1, TLR2, TLR4, and TLR5) and another cluster of FcRs ... were used as the background The median and standard deviation of background levels were calculated Genes whose intensities were less than the median plus two standard deviations of the background...

Ngày tải lên: 09/08/2014, 13:22

... (Table 3) At the end of the first week of the ICU stay, there was only significant elevation of mean value of CRP in septic patients There was no significant change in the mean value of serum leptin, ... immediate diagnosis and prognosis because of the time taken to produce a reaction and the duration of increased serum concentration These facts may explain the lower sensitivity of CRP in the early postoperative ... eight of whom were in septic shock and the other three were suffering from severe sepsis There was no significant difference among the groups, except for SOFA scores at ICU admission and the duration...

Ngày tải lên: 13/08/2014, 20:21

... thermodynamic stability of the Ca2+- and Zn2+-loaded mutants can be retrieved from the analysis of metallation effects within the background of the same mutation (Table 2) With the exception of Zn2+-loaded ... the shape of the denaturation curve Complementary to the CD experiments, the thermal denaturation of S100A2-DCys was followed by FT-IR The absorbance change at the amide I (1600– 1700 cm)1) and ... mechanism of Zn2+- and Ca2+dependent activation of S100 proteins The antagonistic effect of Zn2+ and Ca2+ in the control of S100A2 stability provides a molecular rationale for the action of both...

Ngày tải lên: 30/03/2014, 02:20

... process and involves the expression of TNF by various cells types within inflammatory tissue and of RANKL by T cells and synovial fibroblasts [22-25] On the other hand, these data show that the skeletal ... Repair of local bone erosions and reversal of systemic bone loss upon therapy with anti -tumor necrosis factor in combination with osteoprotegerin or parathyroid hormone in tumor necrosis factor- mediated ... TNF blockade was initiated at the stage of early arthritis and was continued for six weeks We then determined the extent of local bone damage and quantified the area of subchondral bone erosions...

Ngày tải lên: 09/08/2014, 07:20

... takes in account the multifactorial nature of the response prediction The results of this and other biomarker studies look promising, but full confirmation of the biomarker profiles in independent ... responsiveness [9,10] The identification of a proteomic biomarker in this work is an important further step in the direction of response prediction in RA and in the design of a multidisciplinary ... serum cytokine levels in guiding anti-TNF therapy Therapy responsiveness was assessed months after the start of therapy, based on the American College of Rheumatology (ACR) criteria for improvement...

Ngày tải lên: 09/08/2014, 14:21

... participated in the critical revision of the manuscript PW conceived of the study, participated in its design and interpretation, and helped to draft the manuscript All authors read and approved the final ... action of the PDI-related proteins mainly toward the formation of disulfide bonds of proteins [23,24] Among various tissues, the liver contains the largest amount of PDI protein, followed by the ... silastic catheter was inserted into the abdominal cavity for the continuous infusion of LPS at a rate of μL/hour for 20 hours (total dose: μg/kg BW) Following the closure of the incision, the animals...

Ngày tải lên: 13/08/2014, 11:22

... composed of non-executive members of the administrative body and/ or members of the supervisory body of the audited entity and/ or members appointed by the general meeting of shareholders of the audited ... They also pointed out the importance of the implementation of a set of agreed auditing standards, the role of the Audit Committee and the possible adoption of a Code of Ethics to underpin professional ... limited to the members of the audit profession, but is demonstrated equally by all of the members of the financial market These findings suggest the presented hypothesis to be true and present...

Ngày tải lên: 10/12/2016, 15:35

... contains the Rel family and CRE binding 6428 factors involved in the NFjB pathway and other functional related factors, such as p300 and CBP Group D contain factors related to cell cycle and DNA ... PTF ⁄ SNAP complex and TBP related factors [39,51,52] F 57 42% Zn finger domains It contains the 90% of the members of nuclear receptor superfamily (they are Zn fingers also) of the HTFN G 19 31% ... functions, and topological features retain functionality and phylogeny However, the nature of the connections between these factors needs to be understood at the level of the protein domain The global...

Ngày tải lên: 19/02/2014, 07:20

... ECL2B-L, the distance between the Ca atoms of ˚ His93 and Ser27e is 7.25 A, and that between the Ca ˚ atoms of His93 and Asp1 is 12.75 A Among the structures mentioned above, the three amino acids of ... dimer of hTNF-a The strong band at 17 kDa and the two bands at 15.0 and 13.3 kDa became clearer with incubation At 94 h of incubation, the band at 15 kDa became faint, suggesting that the fragment ... kDa and band at 19 kDa, the sequencing failed, because of the blocking of the N-terminus by molecules Band at  17 kDa, corresponding to hTNF-a, gave one main and two minor sequences The main band...

Ngày tải lên: 06/03/2014, 22:21

... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative ... over the proteins, including several serine-rich stretches [9] The 8578 bp cDNA sequence of XHIVEP1 contains 242 bp of the 5¢-UTR, an open reading frame of 7734 bp and 602 bp of the 3¢-UTR The...

Ngày tải lên: 30/03/2014, 13:20

... TRAIL to the liposome suspension containing mM of calcein and 50 lM of phospholipid in the buffer of the respective pH at 25 °C The reaction was stopped after the initiation The leakage rate of calcein ... contribute to the understanding of the intrinsic properties of TRAIL for inducing membrane disruption under acidic environments, as observed in other members of the TNF family More 5286 G H Nam and K ... liposomes and then the fluorescence intensity of calcein was determined to assess the amount of calcein in the liposome RESULTS Structural analyses of TRAIL by CD spectroscopy The effects of acidification...

Ngày tải lên: 31/03/2014, 08:20

... Description and Boundary Conditions of Load-Carrying Cruciform Welded Joint Figure (3) shows the results of mesh sizes density It was noticed that there are no effects on the stability of results and the ... Figure (4) shows the results of a crack increment step (Δa) It was noticed that there are no effects on the stability of results and the very close agreements between the three types of a crack increment ... the crack would propagate Prior to performing the analysis, it was necessary to specify the magnitude of crack increment and also the number of steps over which the crack would propagate In the...

Ngày tải lên: 24/05/2014, 20:40

... investigate the expression profiles and the effects of green tea on the expression levels of the anti-inflammatory Ttp family mRNAs and some of the pro-inflammatory mRNAs known to be regulated by TTP family ... levels of these four forms of genes in the liver and skeletal muscle of rats fed a high-fructose diet known to induce insulin resistance and oxidative stress [25] The CT values of the Ttp family and ... and the internal control Rpl32 mRNAs and the relative ratio of Ttp family mRNAs in the liver and muscle are shown in Table The CT values of Rpl32 in the liver and muscle were essentially the...

Ngày tải lên: 11/08/2014, 08:21

... p65 are found in the oocyte, pre-implantation embryos and in the testis On the other hand, p50 ⁄ Nf-jb1 EST sequences are found in the oocyte and in the testis but not in any of the pre-implantation ... activity of differentially phosphorylated nuclear factor- kappa B J Biol Chem 280, 244–252 31 Lysiak JJ (2004) The role of tumor necrosis factoralpha and interleukin-1 in the mammalian testis and their ... from the F1 fragment that contained the upstream regulation region, exon and kb of the Rnf33 intron; the sequences were cloned in front of the promoter-less luciferase gene of pGL-Basic In the...

Ngày tải lên: 29/03/2014, 00:20

... was granted by the Institutional Animal Care Committee of the University of Zurich and of the Hebrew University of Jerusalem In the clinical part of the study on CHI patients, the mean IL-18 ... wavelength of 405 nm The experimental part of the study was set-up on two different protocols with the aim to assess the TNF-dependent regulation of IL-18 in the murine brain: (1) The first part of the ... Elevation of tumor necrosis factor in head injury J Neuroimmunol 1990, 30:213-217 Ross SA, Halliday MI, Campbell GC, Byrnes DP, Rowlands BJ: The presence of tumour necrosis factor in CSF and plasma...

Ngày tải lên: 19/06/2014, 22:20

... and of IL-17 showed a synergistic effect on the nuclear translocation of C-Fos, of FosB, of Fra-1, of Fra-2, of C-Jun, of JunD and of NF-κB This combination had a particular effect on Fra-1 and ... cells were fixed and stained The dose effect of each cytokine was evaluated by the measurement of the mean green intensity of the nuclear staining with image analysis software The quantitative ... NF-κB translocation The weak effect of IL-17 indicates that it has rather a regulatory effect, leading to the amplification of the action of other cytokines β Contribution of the T-cell-derived...

Ngày tải lên: 09/08/2014, 01:23