interaction between the cell membrane and the organelle membrane
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... binding to its plasma membrane receptor and the activation of the plasma membrane H+-ATPase IV Fusicoccin induces the association between the plasma membrane H+-ATPase and the fusicoccin receptor ... experiments The interaction between PPI1588His6 and the C-terminus of the PM H+-ATPase was tested both by incubating with FEBS Journal 272 (2005) 5864–5871 ª 2005 FEBS Interaction between plasma membrane ... described 5869 Interaction between plasma membrane H+-ATPase and PPI1 in De Michelis and Spanswick [33] The PM H+-ATPase activity was evaluated as the difference between total activity and that measured...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... minus-strand 3¢ UTR for RNA synthesis To characterize the cis-acting sequence at the plus-strand 3¢ UTR for RNA synthesis, RdRp assays containing the Ó FEBS 2004 Interaction between CSFV NS5B and the ... region and the minus-strand 5¢ UTR, similar to the competitive experiment Ó FEBS 2004 towards the plus-genome Interestingly, when the plusstrand 3¢ UTR and the minus-strand 3¢ UTR were used at the ... contribute to the formation of these products A TNTase could add nontemplated terminal nucleotides to either the newly synthesized RNA or the input template Therefore, we further examined whether the purified...
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... ApoTf into the BIAcore channel to determine whether the ferric ions were required for the interaction between gastrins and ApoTf In the presence of EDTA, no interaction between ApoTf and either Gamide ... with the difference in the circulating concentrations of Gamide and transferrin In the present study, we explored further the interaction between Gamide and transferrin, and characterized the interaction ... without the polyglutamate region were used to determine whether the N-terminus or C-terminus of Ggly is required for the interaction between Ggly and ApoTf A typical analysis of the interaction between...
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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx
... of the complex The mutation could also affect the binding of the ISP to the bc1 complex and distort the Qo site Our data showed that, in the mutant, the bc1 activity was severely decreased The ... ISP and the extramembranous cd2 helix of cytochrome b are important for maintaining the structure of the hinge region and, by consequence, the movement of the headgroup and the integrity of the ... enzyme with their ISP in different conformations The mutation G167E could distort the hinge region of the ISP and hinder the movement of the headgroup Mutations in the hinge region of the ISP, which...
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Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
... side of the membrane While in the E2 form the Ca2þ binding sites have translocated across the membrane to the luminal side and are of low affinity Furthermore, in the E1 conformational state, the ... 3A) and ATP (Fig 3B) in the absence and presence of curcumin, using the coupled enzyme assay In Fig 3A, the half-maximal activation of the ATPase by Ca2þ was measured in the absence of and in the ... contact in the absence of ATP, curcumin is then able to bind to the ATPase (possibly at the hinge region) locking the two domains together and therefore precluding ATP binding (i.e inhibiting the ATPase...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... (Life The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS 2002 Interaction between androgen ... of the LBD, respectively (K720 and E897 in AR) [35–37] AR K720 and E897 are both involved in the ligand–dependent interaction between AR LBD and the coactivator TIF2 [9,11,15] However, in the ... by interaction with the charged clamp [5,36,37] The first and last leucine residue in the LxxLL motif enter the hydrophobic cleft in the LBD, and directly contact amino acid residues within the...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... in the D2 domain and D(495–535) in the D3 domain, are involved in the interaction with CD, and that this interaction enhances the catalytic activity of the enzyme Our results show that the interaction ... properties of the enzyme [19] To extend the information about the role of the noncatalytic SBD regions and their effect on the C-terminal CD, we further explored the amino acids of the N-terminal ... blotting and co-expression experiments between the N- and C-terminal domains of SSIII In vitro assays revealed an interaction between the D123 domain and CD Furthermore, when co-expressed in E coli cells,...
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Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot
... that the interaction is phosphodependent and that the effect was caused by PPase masking the binding of the peptide to 14-3-3 More of the g and c 14-3-3 isoforms bound to both the phospho- and ... 6979 Interaction between CKIa and 14-3-3 S Clokie et al 14-3-3 g and c isoforms bind most tightly to both the phospho-peptide and the dephosphopeptide and have been identified both in vitro and ... with the phospho-peptide Therefore, the interaction between centaurin-a1 and CKIa occurs when CKIa is dephosphorylated 14-3-3 isoforms associate with CKIa both in vitro and in vivo To determine the...
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![Báo cáo khoa học: Investigation of the interaction between the atypical agonist c[YpwFG] and MOR docx](https://media.store123doc.com/images/document/14/rc/ht/small_57MlXT7HZ5.jpg)
Báo cáo khoa học: Investigation of the interaction between the atypical agonist c[YpwFG] and MOR docx
... A of the CPPs and complete the binding site walls, although no particular interactions are implicated between these residues and the ligand Again, the complete amino acid composition of the binding ... contribution of the QM ⁄ MM polarization to the ligand, the interaction energy between compound and the environment was calculated Despite the small movements of the binding site residues, the QM ⁄ ... of 3.30 A between the Oe1 and Oe2Glu310 (EL-3) and the OH˚ Tyr1 of compound (3.04 and 3.38 A, respectively) In this orientation, Tyr1 and Phe4 are surrounded by Phe221 and Trp318, and the d-Trp3...
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Báo cáo khóa học: Interaction between the 2¢)5¢ oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1 potx
... dinucleotides and silences gene expression [26–28] Furthermore, the interaction was specific for MBD1, as we failed to detect any interaction between p59 OASL and other members of the MBD family using the ... interaction between LexA–p59.4 OASL protein and prey MBD1–GAL4AD (A) The L40 yeast strain was transformed with the indicated baits and preys and assayed on double and triple selection plates Prey32 and ... of MBD1 in HT1080 cells was verified in lane and lane To confirm that both the full length and the deletion mutant of p59 OASL were stably expressed in the HT1080 cells used in the immunoprecipitation...
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Báo cáo khoa học: An NMR study of the interaction between the human copper(I) chaperone and the second and fifth metal-binding domains of the Menkes protein pot
... two-dimensional HSQC and NOESY spectra of MNK5 with and without the poly(His) tag shows that there is no detectable interaction between the tag and the remainder of the protein and that the solution ... high-resolution NMR the interaction between HAH1 and two different soluble domains of ATP7A: the second (MNK2 hereafter) and the fifth (MNK5 hereafter) The solution structure of both the apo- and copper(I)-form ... that of the chaperone 869 Interaction between HAH1 and ATP7A It has been proposed for the ATP7B protein that the second metal-binding domain constitutes the preferred one for the uptake of the first...
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the interaction between the frequency of market quotes spread and volatility in forex
... Lastrapes (1990) and Laux and Ng (1991) support the second The second relationship also supports the model of Brock and Kleidon (1990) where the link between variations in demand and the variability ... where , sp , and n are the standard deviation of the R R R percentage change of an exchange rate, the average spread, and the number of quotations within the tth halfhour interval, and the system ... 378 Kaul and Lipson (1991), Lamoureux and Lastrapes (1990), Bollerslev and Domowitz (1991)] On the other hand, Lamoureux and Lastrapes (1990) and Laux and Ng (1991) find that the use of contemporaneous...
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Báo cáo y học: " The interaction between the PARP10 protein and the NS1 protein of H5N1 AIV and its effect on virus replication" pot
... increase in the supernatant of BHK21 cells 48 h after the infection, had significant increase in the cells 60 h after the infection, and no significant increase in the supernatant and in the cells ... PARP10 To verify the screening result of yeast two-hybrid system, we identified the in vivo and in vitro interaction between NS1 and PARP10 First, we verified the presence of the interaction with ... phosphorylation and binding RNA polymerase I [25] Synthesized PARP10 in cytoplasm can migrate to nuclei, and this provides a space for interaction between PARP10 and NS1 Therefore, research on their interaction...
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Báo cáo khoa học:" The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment" pdf
... within NTAIL These results support a lack of involvement of the three spin-labeled sites in the interaction and/ or a lack of interaction between NTAIL and IRF-3 RD Although this former hypothesis could ... reflect either a lack of interaction between the proteins, or a location of the IRF-3 RD trp residues outside the region of interaction This latter hypothesis is however unlikely, since the trp ... somehow, the hypothesis can be drawn that a specific cellular context is required for the interaction to occur We therefore questioned whether a bacterial lysate could provide such a context The interaction...
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Báo cáo y học: "Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte" pot
... that the cells of the embryo and suspensor were large, and the suspensor displays a bend due to the irregularly bulged cells (panel i, arrow) (j) The majority of the ila-1 embryos arrested when they ... developing seed [1] The egg, the central cell, and two accessory cell types (specifically, two synergid cells and three antipodal cells) are contained in the embryo sac, also known as the female gametophyte ... expressed in the egg and the central cell, and the expression of the receptor-like kinase gene AT5G60270 was found to be restricted to the egg cell alone (Figure 2) In addition to the in situ hybridization...
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A study on an example of non-verbal interaction between the presenter and audience for English majored students at the School of Foreign Language
... recorder, they can evaluate themselves: they can hear what their voice sounds like and observe their nonverbal communication Body language has the great deal on the interaction between students and their ... the body The body is the largest part of a presentation in which the presenters place their arguments and ideas, their substantiation and examples, and their proofs and illustrations The goal ... perfecting their essential skills and expanding social network One thing which marks the breakthrough of the way the teachers teach their students and the ways the students learn things is the appearance...
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Interaction between the forced and parametric excitation with different degrees of smallness
... it u t in g these v a lu e s in to (2.5 ) an d (2.6) we o b ta in the fo llo w in g re s tric tio n the a m p lit u d e at)' -Ỉ > J 5 • it e p 13 ) A s it w ill be seen la te r, the cu rv e ... tlie a x is d o , we pass from a region w < to a reg io n w t the p o in t of in te rse ctio n betw een the s tra ig h t lin e and the reson ance c u rv e r iv a t iv e d w / d a o is p o sitiv ... m a regio n w > to a region w < O n the c o n tra ry , if < 0, then the p o in t of in te rse ctio n ponding to a sta b le sta te of o s c illa tio n if M < and to an u n sta b le one if h is...
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A microscopic examination of the interaction between antibodies, dengue virus and monocytes
... the landscape and places abandoned the colonial system [Halstead, 2006] Movement of troops during war aided the dispersal of the DENVs between population centres of the Asia-Pacific regions and ... and hence poor housing, water supply and sewerage system [Ooi and Gubler, 2008] As such, people tend to store water in households, and together with the abandoned war equipment and rubbish, these ... vaccine candidate and another that uses DENV-4 attenuated by 30-nucleotide deletion of the 3’ untranslated region as the genetic backbone, with the E and prM genes replaced by that from the other...
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Interaction between the elements with different degrees of smallness in nonlinear oscillating systems
... (2.7) and the further discussion will be th esam e as in the paragraph teraction between the elem ents characterizing the parametric and xcita tions with different degrees of sm allness The noinlinear ... allness The structure o f the equation (3.1) shows that in the first approximation [1] lents orn the right o f (3.1) not affect the oscillation In the second approximation the terms mentioned and ... the trivial solution 11=0 of the equations (3.8) is unstable nteraction between metric excitations the elements characterizing the forced and Let us consider a nonlinear system described by the...
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