integrated af fi nity and electrophoresis systems for multiplexed biomarker analysis
Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
Ngày tải lên :
30/03/2014, 03:20
... phospho -af nity gel electrophoresis Together, the phospho-specific antibody and phospho -af nity gel electrophoresis enabled us to directly study CK2-dependent Cdc37 phosphorylation in vitro and in ... were analyzed by phospho -af nity gel electrophoresis and stained with CBB phospho -af nity gel electrophoresis Phosphorylation was rapid and detected within (Fig 8A, lane 4) and completed within at ... 30 °C for 30 min, analyzed by phospho -af nity gel electrophoresis, and then stained with CBB (B) Wild-type protein (lanes 1, and 7) and two Cdc37 mutant proteins, 13SA (lanes 2, and 8) and 13SD...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
Ngày tải lên :
14/02/2014, 14:20
... The Kd values obtained for the higher -af nity and lower -af nity binding sites are listed in Table The high -af nity and the low -af nity sites accounted for 25% and 75% of the total binding, respectively ... high -af nity (32 nm) and low -af nity (86 nm) states has previously been demonstrated for binding of PCSK9-S127R to the ECD of LDLR at pH 7.5 [25] This study also found highaf nity (1 nm) and low -af nity ... performed in duplicate consistently showed curvilinear plots that implicated the presence of high -af nity and low -af nity sites ⁄ states with af nities for PCSK9 that differ approximately five-fold...
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Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx
Ngày tải lên :
18/02/2014, 06:20
... protein C contains the ‘low af nity Pro10, but did not gain a significant increase in af nity by mutagenesis to P10Q because mutant I (QN) had only a modest 1.5-fold af nity increase compared to ... displaying a different af nity The results obtained in the present study indicate that the high af nity mutants (e.g II and III) can utilize ‘poor’ binding sites that low af nity mutants (e.g wild-type) ... (1.5-fold) gain in af nity compared to wild-type The major af nity improvement came from combined mutagenesis at position 32 ⁄ 33 with mutant V (ED) having a 3.5-fold higher af nity than wildtype...
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
Ngày tải lên :
19/02/2014, 12:20
... [31,32], for example, pYEEI, pYDNV, pYTDM and pYTDL for Src family SH2 domains; pYENP, pYTEV and pYMDL for the Abl SH2 domain; pYDHP, pYKFL and pYNR for the CrK SH2 domain; pYDEP, pYDED and pYDEV for ... pYLNV, pYIN and pYMN for the Sem5 SH2 domain; pYMXM, pYVXM, pYIXM and pYEXM for the N-terminal SH2 domain of p85; pYXXM for the C-terminal SH2 domain of p85; pYVIP, pYILI and pYILV for the C-terminal ... significant contributions to the exposure and positioning of the Tyr304 residue for binding to tyrosine kinases and phosphatases On the other hand, tyrosine phosphorylations not affect the conformation...
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Báo cáo khoa học: Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation docx
Ngày tải lên :
16/03/2014, 19:20
... 272 (2005) 2557–2565 ª 2005 FEBS S100A1 af nity for calcium a calcium chelator also show that the af nity of the N-loop for calcium is very low Both E73Q and E73Q)2-mercaptoethanol bind calcium ... Nevertheless, because formation of mixed disulfide between the subunits of E73Q and 2-mercaptoethanol does not affect the af nity of the protein N-loops for calcium, it can be safely assumed that ... studies of the E73Q mutant and its mixed disulfides ( 102 m)1) This means that the subunits of these proteins bind first to C-loops and then to 2559 S100A1 af nity for calcium N-loops, so that...
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Báo cáo khoa học: Apolipoprotein E-derived antimicrobial peptide analogues with altered membrane affinity and increased potency and breadth of activity pdf
Ngày tải lên :
30/03/2014, 03:20
... carbamate chemistry and purified by HPLC as described previously [12] For peptides apoEdp and apoEdpL-W, peptide weight was confirmed by amino acid analysis, and far-UV CD spectra obtained for peptides ... defensins [3] and LL-37 [4], are often long and complex, making rational modification and testing of their entire sequences difficult, and meaning that such peptides may be too costly for clinical ... concentrations After 48 h of incubation, 25 lL MTT in 0.5% medium was added (1 mgÆmL)1 final concentration), and cells were incubated for h before removal of growth medium and solubilization of formazan...
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nanostructured and photoelectrochemical systems for solar photon conversion, 2008, p.781
Ngày tải lên :
04/06/2014, 14:30
... thank Alexandra Anghel, Carol Burling, Barrie Clark and Stuart Honan for their editorial assistance, David Ginley, John Kelly and Reshef Tenne for providing information, James Bolton for his early ... Nanostructured and Photoelectrochemical Systems for Solar Photon Conversion eds Mary D Archer & Arthur J Nozik Forthcoming From Solar Photons to Electrons and Molecules by Mary D Archer Wanda - Nonostructured ... while the valence-band edge of the absorber must be below that of the hole conductor The conduction-band and valence-band offsets ∆Ec and ∆Ev between the electron or hole conductor and the absorber...
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Báo cáo khoa học nông nghiệp " An Integrated Production, Distribution and Marketing Plan for Melon and Cabbage In Vinh Province, Vietnam " pot
Ngày tải lên :
21/06/2014, 04:20
... ASINCV, RIVAF and province level MARD extension centre and district level extension station staff b Growers and c Retailer SALES & MARKETING: Sales Coordination a Establish sales and marketing ... Posters, leaflets etc (branded) ii In-store sampling iii Metro staff education b Food service customers – tourist hotels and restaurants c Trade e.g ‘Asiafruit’ magazine QUALITY/FOOD SAFETY: Document ... in GAP Manual and communicate to farmers/suppliers (including training) Quality standards required, based on Metro specifications Food safety requirements, based on international standards TRAINING:...
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position-sensitive devices and sensor systems for optical tracking and displacement sensing applications
Ngày tải lên :
03/10/2014, 22:39
... help and support I thank all my co-workers for the pleasant working atmosphere I also wish to thank Markku Koskinen and Esa Jansson from Noptel and Ilkka Kaisto from Prometrics for their help and ... help and for the sincere interest they showed towards my work I wish to thank Prof Erkki Ikonen and Dr Kalevi Hyyppä for examining my thesis, and Mr Malcolm Hicks and Mr Janne Rissanen for revising ... DEVICES AND SENSOR SYSTEMS FOR OPTICAL TRACKING AND DISPLACEMENT SENSING APPLICATIONS Academic Dissertation to be presented with the assent of the Faculty of Technology, University of Oulu, for public...
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cooling and insulating systems for mass aci 207.4r-93 concrete
Ngày tải lên :
24/10/2014, 17:25
... Society for Testing and Materials (ASTM) C 150 Standard Specification for Portland Cement C 494 Standard Specification for Chemical Admixtures for Concrete C 512 Standard Test Method for Creep ... insulation and forming system COOLING AND INSULATING SYSTEMS Stay-in-place precast concrete panels have been used on several RCC dams to form both upstream and downstream faces, and also to form spillway ... exposed edges and comers from excessive heat loss 1.5-Instrumentation Temperature monitoring of concrete components during handling and batching, and of the fresh concrete before and after its discharge...
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Auto dosing and dispensing systems For Dye house
Ngày tải lên :
06/08/2015, 13:08
... available systems Dispensing and automated handling systems for dyes and chemicals have been reviewed and a tabular guide given of the functions available on commercially available systems for which ... chemically resistant and of sufficient weight- bearing capacity to allow for the traffic of heavy containers Walls and floors should allow for easy and regular cleaning Suitable storage for dyes ranges ... given The ColorService TRS automatic weighing and dissolving system for solid dye brands has been described The main advantages include sealed, dry and safe storage of dyes, preventing moisture fluctuations...
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Mechatronics and intelligent systems for off road vehicles
Ngày tải lên :
17/02/2016, 14:48
... to handle (especially for stereo images), and the dependency of such systems on lighting conditions, with notable consequences for reliability and robustness 1.5.7 Thermocameras and Infrared for ... irregularities, and it develops the longitudinal forces needed for traction and braking and the lateral forces required for vehicle cornering (thus ensuring steering control and direction stability) ... knowledge of the draft force required to pull the implement The ASABE Standard 497.6 [10] includes an equation for calculating this draft force for a range of different implements for which a set...
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Báo cáo hóa học: " Research Article Combination of Accumulated Motion and Color Segmentation for Human Activity Analysis" ppt
Ngày tải lên :
22/06/2014, 06:20
... 100 from fight Activity area for fight 2, frames 31–150 Activity area for fight 2, frames 150–200 (a) (b) (c) MEI for fight 2, frames 31–150 MEI for fight 2, frames 150–200 Activity area for walking ... and differences, as the curvature zero-crossings for pose are 10 EURASIP Journal on Image and Video Processing maximized after more stages than for poses and 3, namely after 48 instead of 29 and ... frame 100 Activity areas fight 2: (b) fighting, (c) walking MEIs fight 2: (d) fighting, (e) walking (f) Activity area for walking (g) MEI for walking Activity areas for walk and fall: (h) walking,...
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Báo cáo khoa học: "Improved survival following surgery and radiation therapy for olfactory neuroblastoma: analysis of the SEER database" pdf
Ngày tải lên :
09/08/2014, 09:20
... site and year of diagnosis The SEER historic staging variable provided information for the following categories: localized, regional, distant and unstaged Information for type, timing, and duration ... contributions MEP and TLM reviewed the SEER database for OFN cases and performed the statistical analysis MEP, TLM, MM, and AKS wrote the manuscript All authors reviewed the statistical analysis results, ... http://www.ro-journal.com/content/6/1/41 stratified by treatment was: 73% for surgery and radiotherapy, 68% for surgery only, 35% for radiotherapy only, and 26% for neither surgery nor radiotherapy There was a significant...
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Báo cáo y học: "A simpler method of preprocessing MALDI-TOF MS data for differential biomarker analysis: stem cell and melanoma cancer studies" pptx
Ngày tải lên :
13/08/2014, 13:21
... data Therefore, we used GANN and stepwise ANN for feature selection and classification GA and ANN are two widely used methods for handling noisy and complex data We applied MCCV and random sampling ... achieved FPR of 7.23% and 8% for L (low) and H (high) groups, respectively We also performed ROC analysis based on the classification performance of each sample in 50 random sampling and > 0.9 AUC values ... algorithms are robust for handling noisy data and cost effective for candidate marker selection For future work, studies of biomarker validation on the identified panels will be performed to support...
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Registration and segmentation methodology for MR image analysis application to cardiac and renal images 1
Ngày tải lên :
11/09/2015, 10:17
... Different performance measures for cardiac image registration (a) average registration error in mm for all 12 cardiac datasets; (b) mutual information values before and after registration for all ... and corresponding image patch; and (c)(d) intensity values after contrast enhancement and corresponding patch 3.6 53 Robustness performance for (a) rotation and ... saliency information; GI-only gradient information; and GSI-combination of gradient and saliency information MI-mutual information (no units); WC-Woods criteria value in (3) (no units); and Errordisplacement...
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Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
Ngày tải lên :
07/03/2014, 05:20
... its af nity constants for peptide transporters are only in the medium range, with Kt values of 1.3 mm for PEPT1 [17] and 108 lm for PEPT2 [18] New high -af nity labeled probes are required for ... uptake, whereas the af nity constant of Bip-Pro uptake is 12-fold lower Bip-Pro uptake represents a high -af nity, lowcapacity process, whereas the Gly-Sar uptake occurs with low af nity but high transport ... by Gly-Gln and the af nity of PEPT2 was slightly lower for GlySar (Kt ¼ 0.3 mm) than for Gly-Gln (Kt ¼ 0.1 mm) The situation is very similar for PEPT1, where Bip-Pro elicited 21% and Gly-Sar...
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Báo cáo khoa học: Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans potx
Ngày tải lên :
15/03/2014, 23:20
... quantitative analysis of sugar– protein interactions, in terms of af nity constants (Kd or Ka), of DC-SIGN, DC-SIGNR and LSECtin Previously, we developed an automated frontal af nity chromatography ... showed the lowest af nity among the three C-type lectins, while LSECtin–Fc showed the highest af nity under the present experimental conditions In terms of specificity, DC-SIGN–Fc and DC-SIGNR–Fc ... 8C and D, dTHP-1 and HL-60 cells adhered to Lec8 cells Cell adhesion was specifically blocked by pretreatment with mAbs specific for DC-SIGN and LSECtin (by approximately 30% for dTHP-1 cells and...
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Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx
Ngày tải lên :
16/03/2014, 16:20
... that of the unmodified enzyme The results are shown in Table The modified enzyme exhibits about threefold reduced af nity for GSH and about twofold increased af nity for CDNB A final activity of ... carboxy and primary amino groups for the modified and unmodified enzyme were determined by the Woodward’s Reagent K and 2,4,6-trinitrobenzenesulfonic acid assays Unmodified SDTG-modified SDTG-modified ... GSH was more significant than that afforded by S-methyl GSH, at comparable concentrations, which is in agreement with their relative af nity constants Kinetic analysis of the modified enzyme (42%...
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Báo cáo khoa học: Selective modulation of protein C affinity for EPCR and phospholipids by Gla domain mutation pdf
Ngày tải lên :
16/03/2014, 18:20
... type II deficiency phenotype [50], displayed only a small reduction in af nity for sEPCR and a similar Km for endothelial cell activation by thrombin to that of wild-type protein C (Tables and 3) ... the figure are representative sensograms for each set of experiments defined values [12,44] The fitted inactivation data (Fig 4B) and the kinetic rate constants for FVa inactivation by Q32A, V34A and ... smeared bands resolved into a single defined band (data not shown) Variant forms of sEPCR were generated by site-directed mutagenesis (N30Q, L37A and E86A) and were expressed, concentrated and bufferexchanged...
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