inside out and outside in

... by controlling medium inflow and outflow In other words, engineers can provide steady-state conditions for cells, as well as fast and predictable changes in the environment surrounding the cells ... differentiation in vitro on microfluidics platforms produces more standardized outcomes Many investigators believe that the stress of manual handling of hESCs is an important factor in their instability ... and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) are particularly sensitive to handling in culture, and automation of hESC growth and...

Ngày tải lên: 06/08/2014, 19:21

... family and, in most animals investigated, are expressed specifically in the germ line, where they are thought to function in silencing transposons and translational gene regulation In Schmidtea and ... understanding the functioning of these remarkable stem cells However, current research in stem-cell biology is providing increasing evidence that the behaviors and fates of stem cells are not inherent, ... expression and function in neoblasts Robust methods for in situ hybridization and gene knockdown via RNA interference (RNAi) have now been developed for both platyhelminths and acoels, and these...

Ngày tải lên: 06/08/2014, 19:21

... mesodermal lineage cells in vivo, including blood, cartilage, and skeletal and cardiac muscle cells In addition, wild-type or genetically corrected mesoangioblasts delivered into the arterial blood ... Encouraging results were obtained after 10 months of follow-up An international phase II trial including 300 patients is in progress and the first results of this will be available in 2006; the trial is ... using increasingly lineagerestricted donor bone-marrow cell populations This work has shown that fusion occurs in mouse liver between host hepatocytes and transplanted macrophages Further insight...

Ngày tải lên: 14/08/2014, 14:21

... proteins (enzymes, immunoglobulins) from hydrolysis in milk and in intestine of newborn (Hamosh 1995) Sulfhydryl Oxidase Maintain structural and functional integrity of milk proteins, enzymes and ... assist in controlling of infections in the stomach and small intestine (Hernell et al 1989) Phospholipids in HBM, can be subdivided into major classes: phosphatidylcholine, phosphatidylethanolamine, ... 1997) In addition to being a mitogen for T lymphocytes in culture (by induction of interleukin-2 and interleukin-2 receptors (Ellis and Picciano 1992; Grosvenor et al 1993), milk-borne prolactin...

Ngày tải lên: 11/09/2015, 09:57

... Tan Evan Stephen and Mr Ang Wee Siong for helping with various technical, administrative and safety issues and Ms Siew Woon Chee for helping out in operating the rheometer Finally, I would like ... used in tissue engineering is gelatin Gelatin is a natural polymer obtained from collagen upon acid or alkaline processing Gelatin is biodegradable, biocompatible and has been in regular usage ... characterization vi 3.2.6 Oil red O staining 34 3.2.7 Alizarin red staining 34 3.2.8 Real-time quantitative polymerase chain 35 reaction 3.2.9 Immunofluorescence staining 3.2.10 In vitro HUVEC - matrigel...

Ngày tải lên: 09/09/2015, 11:18

... developing a screening system to identify and characterize candidate insecticidal compounds showing both inductive and potentiative activity Results The DNA-binding domains (DBDs) of Leptinotarsa and ... 3729–3734 Supporting information The following supplementary material is available: Fig S1 Amino acid alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors Fig S2 Amino acid ... structure of a hemipteran ecdysone receptor ligandbinding domain: comparison with a lepidopteran ecdysone receptor ligand-binding domain and implications for insecticide design J Biol Chem 280, 22258–22269...

Ngày tải lên: 18/02/2014, 08:20

... Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the ... public, and the scientific and engineering communities The Council is administered jointly by both Academies and the Institute of Medicine Dr Ralph J Cicerone and Dr Wm A Wulf are chair and vice ... Austin, Texas KENNETH F KELLER, University of Minnesota, Minneapolis, Minnesota RANDALL MURCH, Virginia Polytechnic Institute and State University, Alexandria, Virginia GREGORY A PETSKO, Brandeis...

Ngày tải lên: 06/03/2014, 15:20

... Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis Nat Cell Biol 6, 207–214 Kosinski PA, Laughlin J, Singh K & Covey LR (2003) A complex containing polypyrimidine tract-binding ... MR & Hardy S (2004) Polypyrimidine tract-binding protein is involved in vivo in repression of a composite internal ⁄ 3¢-terminal exon of the Xenopus alpha-tropomyosin Pre-mRNA J Biol Chem 279, ... insights into gene regulation through mitosis Another interesting approach would be to clarify the significance of PTB in cells without a mitotic cycle Heart and brain tissues may be interesting...

Ngày tải lên: 07/03/2014, 00:20

... suspended in PBS were cytospun on slides and kept at -80°C until staining Immunohistochemistry staining Immunohistochemical staining of nestin and BrdU were performed to assess the property of NSC in ... Beijing, China, http://www.vitalriver.com.cn) at a mean age of 14 weeks and body weight between 240 and 260 g were maintained on a 12-hour light/dark schedule and randomly assigned to the following ... isolated and ligated using a 10-0 suture After covering the burr hole with a piece of gelatin sponge, Page of 10 the temporalis and overlying skin were sutured Animals were then placed in the supine...

Ngày tải lên: 18/06/2014, 16:20

... transcriptase(Invitrogen) and incubated at 50°C for 60 minutes followed by incubated at 70°C for 15 minutes Single stranded RNA was digested from DNA/RNA hybrid by adding 0.5 µL of RNaseH and incubated ... Differentiation of varicella-zoster virus ORF47 protein kinase and IE62 protein binding domains and their contributions to replication in human skin xenografts in the SCID-hu mouse J Virol 2003, 77(10):5964-5974 ... L, Sommer MH, Heineman TC, Cohen JI, Kaneshima H, Arvin AM: The ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu mouse...

Ngày tải lên: 20/06/2014, 01:20

... quantifying GFP expression and distinguishing the fluorescence intensity of individual cells in a small cell population Chemicals but especially particles can interact with single cells within a ... which contains genes coding for ampicillin and neomycin resistance, allowing selection in respective bacteria and after transfection in human cells Essential is that the construct also contains the ... 10 of 14 L-glutamine (4 mM), penicillin (100 U/ml), streptomycin (100 μg/ml), and 10% (v/v) FCS) in the presence of G418 (0.5 mg/ml final concentration) Cells were maintained in a 5% CO2 humidified...

Ngày tải lên: 21/06/2014, 00:20

... stay in contact with the spill for 30 Drain the contents of the catch basin into a container suitable for final sterilization by autoclaving Visually inspect the working interior of the laminar ... Prepare and maintain the work area Perform all aseptic work in a clean work space, free from contaminating air currents and drafts For optimal environmental control, work in a laminar-flow cabinet ... cabinet air before starting work While working, keep all material and perform work ≥4 in back from the front opening of the cabinet, and minimize rapid movements or activity Keep the view window...

Ngày tải lên: 11/07/2014, 21:20

... probably prudent to determine outcome with less intense regimens before testing more intense, and potentially more toxic, conditioning regimens Conclusion Future studies are being planned to confirm ... avascular necrosis and for occult infection prior to registration In addition, aggressive antiviral, fungal and bacterial coverage is initiated during any period of neutropenia independent of fever ... comparison of HSCT with cyclophosphamide and ATG versus the Hopkins’ treatment protocol incorporating high-dose cyclophosphamide without stem cell support Competing interests None declared References...

Ngày tải lên: 09/08/2014, 01:23

... equally effective in inducing stable chimerism, while maintaining efficacy [12,15] Of note, no GVHD was observed, indicating that the putative graft-versusautoimmunity effect and GVHD are dissociated ... survival and reduced proteinuria, immune complex formation, and antinuclear antibodies (ANAs) While the aforementioned preclinical studies have been instrumental in demonstrating basic principles ... antigens DCs, specialised in presenting antigens to donor effector cells, are mainly involved in this setting and probably also in autoantigen (cross)presentation in AD In most tissues, recipient DCs...

Ngày tải lên: 09/08/2014, 10:23

... Dscr1Rcan1 and the protein kinases Pdxk and Pfkl Finally, one transcription factor (Olig2) and three protein kinases (DYRK1A, SNF1LK and Ripk4) were tested at three different time points (17, 24, and ... HSA21 genes, including Dyrk1a, Synj1 and Sim2, results in learning and memory defects in mouse models, suggesting that trisomy of these genes may contribute to learning disability in DS patients ... a certain property of the encoded protein, called intrinsic disorder [25] The protein disorder is defined as the total number of amino acids included in unstructured regions of the protein These...

Ngày tải lên: 09/08/2014, 20:22

... also being labor-intensive and time consuming The present investigation was aimed at characterizing genetically diverse HIV-1 strains circulating in Cameroon in terms of co-receptor usage and comparing ... attending clinics in three cities in Cameroon - Bamenda, Limbe and Buea Demographic information was collected in the Performa and analyzed Viruses were propagated in PBMC derived from buffy coats and ... Program for providing us cells and chemokine blockers The findings and conclusions in this article have not been formally disseminated by the Food and Drug Administration and should not be construed...

Ngày tải lên: 10/08/2014, 05:21

... used during the conditioning, prevented hyperbilirubinemia [8] It has been reported that UGT1A1 inducers, including phenobarbital, rifampin and phenytoin, were used in the diagnosis and treatment ... daunorubicin, idarubicin, and cytarabine prior to HSCT This may suggest that fludarabine and busulfan had low liver toxicity in GS patients [7] It could also be possible that phenytoin, an inducer ... transaminase and bilirubin levels were almost normal during the conditioning and within 100 days after transplantation, although indirect hyperbilirubinemia repeatedly happened during chemotherapy...

Ngày tải lên: 10/08/2014, 21:23

... cellular therapy in order to enhance engraftment and reduce GVHD while preserving GVL effects Preliminary experience using expanded Tregs in humans is promising, although clinically meaningful expansion ... complex and expensive in time and materials, which need to be improved and simplified before routine use in clinical transplant centers Second, despite the low number, the complete elimination ... transplantation in adults using CD3/ CD19depletion and reduced intensity conditioning; an update Blood Cell Mol Dis 2008, 40:13-19 Handgretinger R, Chen X, Pfeifer M, et al.: Feasibility and outcome...

Ngày tải lên: 10/08/2014, 22:20

... adhesion regulating proteins of the integrin family, and play a role in promoting cell adhesion and spreading, mimicking the effect of some ECM proteins such as fibronectin or vitronectin [13-15] ... the protein adsorption on hydrogel surface and integrin-binding of agmatine containing hydrogels was investigated Adhesion experiments with media containing only 0.1% of FBS were carried out After ... presentation of integrin ligands in a clusterized form enables the formation of focal contacts and stress fibers [37] and determines cell spreading [38] A uniform low density of integrin ligands, instead,...

Ngày tải lên: 11/08/2014, 00:22