insertional inactivation of an antibiotic resistance gene

... M.Goodman, Jo Handelsman (2004) Uncultured soil bacteria are a reservoir of new antibiotic resistance genes Environmental Microbiology.6(9), 981-989 Nwosu, V.C (2001) Antibiotic resistance with ... Kevin S Lang, Janet M Anderson, Stefan SchWarz, Lynn Williamson, Jo Handelsman, and Randall S Singer (2010) Novel Florfenicol and Chloramphenicol Resistance Gene Discovered in Alaskan Soil by ... ligase (3U/ml) 2µl 2µl 2µl Screening transformants for antibiotic resistance clones Transformant were replicated on to LB-Amp and LB-Amp containing an additional antibiotic with MIC: 50µg.ml-1 streptommycin,...

Ngày tải lên: 28/05/2016, 14:41

... information on a wide range of potentially relevant issues) and allows easy administration of general instructions in any language It has the inherent advantage of determining and rating comments ... 13 Calman KC: Quality of life in cancer patients an hypothesis J Med Ethics 1984, 10:124-127 Tully MP, Cantrill JA: The validity of the modified patient generated index–a quantitative and qualitative ... of postpartum care Journal of Obstetrics and Gynaecology of India 2006, 56:142-145 Chandran M, Tharyan P, Muliyil J, Abraham S: Post-partum depression in a cohort of women from a rural area of...

Ngày tải lên: 18/06/2014, 19:20

... Solanaceous plants and the DUF623 domains of AtOFP7, AtOFP8 and AtOFP6 AtOFP7 was found to exhibit analogous function to AtOFP1, which is a known transcriptional repressor of AtGA20ox1 [5] AtOFP1 is categorized ... and cell pattern in the Arabidopsis embryo sac [15] The two subfamilies, the one with CaOVATE, AtOFP6, AtOFP7 and AtOFP8 and the other with AtOFP1, AtOFP2, AtOFP3 and AtOFP5, have a significant ... first and larger of 613bp and the second, and smaller, of 395-bp The unique intron of the gene consists of 539-bp After the stop codon, a 3’ UTR of 66-bp and poly-A tail follow The genomic organization...

Ngày tải lên: 11/08/2014, 11:21

... Figure analysis of methyl halides, methanethiol, and DMS from R sativus GC-MS GC-MS analysis of methyl halides, methanethiol, and DMS from R sativus (a) GC-MS spectrum of methyl halide standards ... these plants and to clarify the mechanism of synthesis of these compounds The emission rates of CH3I from these plants in the presence of mM iodide were 0.4 (T aestivum), 3.1 (O sativa), and 30.8 ... the emission profiles of methyl halides from some plants and the characterization of the enzymatic properties of recombinant R sativus HTMT (RsHTMT) Results and discussion Wuosmaa and Hager [14]...

Ngày tải lên: 12/08/2014, 03:21

... of airway samples consisted of rapid Gram-staining and semi-quantitative culturing of tracheal aspirate On clinical request (in cases of discrepancy between likely clinical diagnosis of VAP and ... systems [29] Antibiotic prescription on diagnosis of VAP was noted: antibiotic prescription on the same calendar day and the calendar day after clinical diagnosis of VAP was considered as antibiotic ... isolation of MDR versus other pathogens in our study behaved as a marker of a category of patients with a lower a priori chance of ICU survival The lack of association between antimicrobial resistance...

Ngày tải lên: 13/08/2014, 11:23

... field can results large changes in the source parameters The three solenoid coil system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and ... code, Reference manual, LA-UR-87-126, LANL 1987 Jain S K, Jain A, Sharma D, Hannurkar P R 2006 Acquisition and analysis of Langmuir probe characterization for ECR plasma Indian J Phys 80: 1011–1015 ... Make:Megger, Biddle, England) Magnetic field mapping The performance of an electromagnet gets influenced by the design and material limitations, errors in construction, and the stability of the power supply...

Ngày tải lên: 22/12/2013, 08:58

... genes besides the SCO2299 gene [26] One is the SCO5812 gene, encoding an RNase HII-like amino acid sequence, and the other is the SCO7284 gene, encoding an RNase HI-like sequence The result of ... 200 lm PIPES as an internal standard, and lg of protein samples in a final volume of 100 lL The reaction mixture was incubated for 10 at 37 °C and transferred to 400 lL cold methanol The contents ... caffeine and ethyl methanesulphonate Implications for roles of RNases H in DNA replication and repair Genes Cells 5, 789–802 Rumbaugh JA, Murante RS, Shi S & Bambara RA (1997) Creation and removal of...

Ngày tải lên: 19/02/2014, 18:20

... 1.52–1.53 1.50–1.54 1.50 1.54 No adduct 1.58 1.59 of cysteine of glutathione of of of of of N-acetylcysteine CoA lipoate ethanol formate of cysteine of glutathione Methyl• 2.28–2.29 1.93 1.70–1.73 ... (1999) ESR and HPLC-EC analysis of the interaction of hydroxyl radical with DMSO Rapid reduction and quantification of POBN and PBN nitroxides Anal Chem 71, 715–721 87 Ross, D., Albano, E., Nilsson, ... Site-directed mutagenesis of dihydrolipoamide acetyltransferase and post-translational modification of its lipoyl domains In Biochemistry and Physiology of Thiamin Diphosphate Enzymes (Bisswanger, H &...

Ngày tải lên: 21/02/2014, 01:21

... of tryptophan was performed in phosphate buffer solution in the presence of tert-butanol (500 mM NaN3, 500 mM tert-butanol) The presence of tert-butanol induced a broadening and a red-shift of ... suggesting quantitative oxidation of TyrOH by Trp , as for other proteins However, the intramolecular rate constants of charge transfer differed by a factor of two between WT Trx and the D30A mutant (Table ... spectrum of the W35A mutant form of Trx obtained 50 and 300 ls after the pulse Among the transients that were formed by oxidation of W35A, the Trp 510 nm broad band was not detected anymore;...

Ngày tải lên: 07/03/2014, 16:20

... Inactivation of antibiotics and the dissemination of resistance genes Science 264, 375–382 Ginns CA, Browning GF, Benham ML, Anderson GA & Whithear KG (1996) Antimicrobial resistance and epidemiology of ... ceftazidime and nitrofurantoin) (Table 1) By , contrast, higher resistance rates to these drugs were noted in organisms isolated from human patients This suggests that the extent of resistance to an antibiotic ... enterohemorrhagic and enteroadherent Journal of Infectious Diseases 155, 377–390 Linton AH (1977) Antibiotics, animals and man An appraisal of a continuous subject In: Antibiotics and Antibiosis in...

Ngày tải lên: 08/03/2014, 09:20

... GOB-18 variant [18] The mutants of GOB-1 generated by site-directed mutagenesis of Gln116 exhibit a loss of activity that cannot be corrected by the addition of zinc The Q116H mutant and the wild-type ... discarded the presence of bound iron in all the enzymes Kinetic study MS of GOB-1 mutants Native ESI-TOF MS spectra of the mutants were obtained Although Fig reveals the presence of many salt peaks, ... wild-type and mutant enzymes measured by ESI-TOF MS and calculated from their amino acid sequences The calculated difference between measured masses of the denatured and native wild-type and mutant...

Ngày tải lên: 14/03/2014, 23:20

... studied and used More comprehensive surveys of data-driven paraphrasing techniques can be found in Androutsopoulos and Malakasiotis (2010) and Madnani and Dorr (2010) The first family of approaches ... evaluation Each sentence pair received a minimum of 1, a median of 3, and maximum of annotations The raw agreement of the annotators (after filtering) was 77% and the Fleiss’ Kappa was 0.43, which signifies ... better understanding of the strengths and weaknesses of each type of approach The evaluation also brings to light additional properties, including the number of redundant paraphrases generated,...

Ngày tải lên: 30/03/2014, 21:20

... Chicago, 1976 [Mann 82a] Mann, W C., et al., "Text Generation," American Journal of Computational Linguistics , (2), April-June 1982 ,62-69 [Mann 82b] Mann, W C., The Anatomy of a Systemic Choice, ... experiment, a test of the functionalism of the systemic framework, and of its identification of the functions of English [Halliday 76b] Halliday, M A K., System and Function in Language, Oxford ... grammar and its environment interact environment The environment is regarded composed of three disjoint regions: informally as being An inquiry consists of an i n q u i r y operator and a sequence of...

Ngày tải lên: 31/03/2014, 17:20

... GPs and practice assistants to cover: patient enrolment and obtaining informed consent, an explanation of the handling of the QL-recorder to participating patients, interpretation and use of results ... aspects of quality -of- life-measurement: design and feasibility study of the quality of life recorder and the standardized measurement of quality-oflife in an outpatient clinic Cancer Treat Rev 1996, ... assistants and GPs This work was supported by the German Ministry of Education and Research [grant number 01GK0201] Page 10 of 11 (page number not for citation purposes) Health and Quality of Life...

Ngày tải lên: 18/06/2014, 18:20

... seuquence and its deduced polypeptide and results of these analyses are shown in Figs (5 and 6) In case of Polh-cr nucleotide seuquence, a phylogenetic tree was generated from sequence data of 38 ... subject in many companies The full length of polyhedrin gene from lepidopteran NPVs ranged from 483 bp to 747 bp [39] In case of polyhedrin gene from Spodoptera sp NPVs, its full length ranges from ... infections and may be important in controlling (preventing/enhancing) baculovirus infection Also, it may be useful in monitoring the distribution of NPVs, the fate of genes and release of wild type...

Ngày tải lên: 20/06/2014, 01:20

... means In Section 3, we give the proof of positive semidefiniteness of matrices generated by the difference of that inequality obtained from the generalization of Mitrinovi´ -Peˇ ari´ inequality and ... Journal of Inequalities and Applications 17 References D S Mitrinovi´ and J E Peˇ ari´ , “Generalizations of two inequalities of Godunova and Levin,” Bulletin c c c of the Polish Academy of Sciences, ... side of 2.49 is mean, then for distinct s, t ∈ R it can be written as Ms,t t s 1/ t−s 2.52 12 Journal of Inequalities and Applications as mean in broader sense Moreover, we can extend these means,...

Ngày tải lên: 21/06/2014, 07:20

... about 60% of oleic acid and 15% of saturated fatty acids Impact of these drying properties has been investigated regarding boron retention and biological resistance of wood to degradation organisms ... H3BO3 0,25 % 0,5 % (H) % of mass loss % of mass loss 25 16 14 12 10 Control % of mass loss % of mass loss 25 (E) % of mass loss % of mass loss 25 16 14 12 10 % of mass loss % of mass loss 25 Control ... Tamashiro M., Resistance of boratetreated Douglas-fir lumber to formosan subterranean termites, For Prod J 42 (1992) 61–65 [7] Hamilton S.J., Buhl K.J., Acute toxicity of boron, molybdenum and selenium...

Ngày tải lên: 07/08/2014, 16:21

... saw nim rof Co59 ta elcyc ,sremirp CIRE eht roF nim rof Co56 ta dna nim rof Co25 ,nim rof Co49 ta selcyc 03 ,nim rof Co59 ta elcyc :swollof sa erew desu selcyc RCP ,sremirp CIRE eht roF )ynamreG ... sisylanA gnisu dezylana erew etalosi hcae fo AND dimsalp dna stcudorp RCP-per rof snrettap dnaB )aeroK ,reenoiB( tik noitalosi AND dimsalp gnisu siserohportcele leg egoraga dna noitcartxe AND ... 01 rof Co4 ta deserohportcele erew sleg ehT leg esoraga %2.1 no detarapes erew stcudorp RCP fo lm 01 ,snoitcaer retfA nim rof Co56 ta dna nim rof Co35 ,nim rof Co49 ta selcyc 03 yb dewollof saw...

Ngày tải lên: 07/08/2014, 18:21

... dezylana saw stcudorp noitcaer eht fo htfif enO Co4 neht nim 01 rof Co27 ta pets noitagnole lanoitidda na yllanif ;ces 03 nim rof Co27 ta noitagnole dna ,ces 04 rof Co06 ta gnilaenna ,ces 04 rof ... rof Co06 ta gnilaenna ,ces 04 rof Co59 ta noitarutaned fo selcyc 03 ;nim rof Co49 ta noitarutaned laitini :snoitidnoc gniwollof eht ta ,)ANDc ro AND cimoneg( xirtam fo stnuoma elbairav dna ,remirp ... lotisaraP spuorg egaknil fo noitanimreted rof sretcarahc citamyzneosi htiw noitanibmoc :sniarts iidnog amsalpoxoT fo noitaziretcarahc laudividni rof dohtem gnitnirpregnif AND A P samohT-esiorbmA ,M...

Ngày tải lên: 07/08/2014, 18:21

... (29.8% and 14.1% respectively) [23] On the other hand, rate of resistance against these antibiotics in countries like Senegal (77% and 55%), Spain, (65% and 33%), Taiwan (80% and 56%), and Israel ... A: Choice of antibiotic for empirical therapy of acute cystitis in a setting of high antimicrobial resistance Indian J Med Sci 2006, 60(2):53-8 Goldstein FW: Antibiotic susceptibility of bacterial ... prevalence of UTI than men, principally owing to anatomic and physical factors [8,17] Antibiotic resistance is a major clinical problem in treating infections caused by these microorganisms The resistance...

Ngày tải lên: 08/08/2014, 19:20