injury body weight and fluid content

... μL and the liquid was followed immediately by 300 μL of air, to ensure delivery to the distal airways and were killed after 7, 14 and 21 days by pentobarbitone overdose Measurement of fluid content ... antiP-selectin antibody (BD Pharmingen, CD62P 1:500), anti-ICAM-1 antibody (BD Pharmingen, CD54, 1:500), anti-nitrotyrosine antibody (1:500 in PBS, v/v), or PAR antibody (1:500 in PBS, v/v) and anti-TGF-b ... The severe lung injury caused by bleomycin administration was associated with a significant loss in body weight, while AM treatment significantly attenuated the loss in body weight (Figure 1K)...

Ngày tải lên: 12/08/2014, 13:22

... immunoprecipitation: polyclonal-antibody directed against the subtypespecific N-terminus of PPARb (lane 2), polyclonal antibody against FLAG (lane 3) and monoclonal antibody against FLAG (M2, lane 4) ... PPARb-specific antibody (upper panel) or the M2 antibody (lower panel) This experiment clearly showed that FLAG-PPARb was precipitated by the PPARb-specific antibody (lane 2), and vice versa, that ... histidinetagged ubiquitin (Ubi-His; lanes and 4) The cells were either treated with GW501516 (lanes and 4) or left untreated (lanes and 3) Cells were harvested after 24 h and analyzed by immunoblotting...

Ngày tải lên: 30/03/2014, 03:20

... seawater and an additional days in RPMI ⁄ DMEM ⁄ seawater) in the absence (A and B) or presence of 30 lM silicate and 10 lM ferric citrate (C and D) Spicules can be seen in the rim surrounding the body ... use grids and analyzed with a Tecnai 12 microscope (FEI Electron Optics, Eindhoven, the Netherlands) Preparation of spicules and axial filaments Silica content of primmorphs Spicules and their ... 150 200 250 the amino acids glutamine, lysine and histidine at the N-terminus and the nonpolar amino acid proline and the hydroxy amino acids serine and threonine at the C-terminal border 3842...

Ngày tải lên: 30/03/2014, 20:20

... long and 1-2.3 µm wide (Hooper and Van Soest 2006; Leys and Degnan 2001) Embryo, larvae and post-larvae possess only one type of spicule, the oxea, being approximately 25 and 40 µm long, 0.5 and ... bones and teeth and brachiopode shells, 3) and silica, which forms the skeleton of most sponge, diatom, silicoflagellates, some choanoflagellates, chrysophytes, synurophytes and radiolarians, and ... (GBR) (Hooper and Van Soest 2006), but its distribution extends to One Tree Island and Magnetic Island reefs (Degnan et al 2008a), related populations have been found in Japan and the Red Sea...

Ngày tải lên: 13/10/2015, 14:07

... micro-model, the macro-model and their interconnection 10.1 Micro- and macro-levels of analysis The contrast between micro- and macro-levels of analysis is often invoked in sociology, and much is made about ... thus be strong, well ordered and systematic, involving intensive and repeated interactions among constituent micro-elements, or weak and rather chaotic, involving few and A general framework 187 ... itself and depends upon the speci®c way in which all of the ®rms of that society are organized and interconnected All capitalist societies will have state apparatuses and private ®rms, and among...

Ngày tải lên: 01/11/2013, 07:20

... and through possession of skills and expertise If class locations de®ned in this way systematically shape the material interests and lived experiences of individuals, and if these interests and ... managers and experts On the one hand, petty bourgeois are owners of the means of production and thus have a clear stake in private property; on the other hand, they are often threatened and dominated ... class categories: comparisons of state and private sectors and of union and nonunion members Consciousness and formation 237 the difference between state and private sector middle-class employees...

Ngày tải lên: 01/11/2013, 07:20

... (PscG and PscE) and polymerizes to form the T3SS needle (B) The V antigen PcrV is released from its cytoplasmic partner (PscG) and forms the cap of the PscF needle (C) Translocator proteins PopB and ... SycD, PcrH and IpgC are shown in yellow, green and magenta, respectively The peptides located within the concave regions of PcrH and IpgC, corresponding to sections of the N-termini of PopD and IpaB, ... targeting and pore formation by the T3SS (YopB) and pathogenic Escherichia spp (EspD) all carry two predicted TM regions, and are predicted to have a N-terminal coiled-coil region and, occasionally,...

Ngày tải lên: 14/02/2014, 22:20

... fat body, gut, integument and whole body of L decemlineata larvae at day of the last (4th) instar A 12.4-kb message was detected by the LdEcR common probe in the integument and whole body, and ... LdEcR and LdUSP (A) LdEcR mRNA and LdUSP mRNA expression in the fat body (FB), gut (GUT), integument (INT) and whole body (WB) at day in the last larval instar, in the INT at day 0, 2, 4, and in ... the second and the third nested PCR were performed with LdEcR-F2 and LdEcR-R2 primers (52 °C) and with LdEcR-F3 and LdEcR-R3 primers (46 °C), respectively The first PCR with LdUSP-F1 and LdUSP-R1...

Ngày tải lên: 07/03/2014, 21:20

... Identical and similar residues within the TRP family are shown in dark and light green, those within the TTR family in dark and light blue, and those within the TLP subgroup in dark and light ... regions are found at sites corresponding to b-strands B and E in TLPs, and to a-helix E in TTRs and TRPs (B) Phylogenetic tree of TRPs, TTRs, and TLPs The tree was based on the multiple sequence ... properties of sbTTR and ecTRP in vitro, and compared the results with those of hTTR Our results showed that hTTR, sbTTR and ecTRP can form fibrillar structures, but under different solution and temperature...

Ngày tải lên: 16/03/2014, 01:20

... 2-oxoacids and 2-Oxoacid dehydrogenase complex from the Archaea pyruvate [14] In the current paper, we report the cloning and expression of the E2 and E3 genes of the same operon from T acidophilum, and ... restriction endonuclease digestion for E2, and NheI ⁄ EcoRI digestion for E3, and purified by gel electrophoresis and gel extraction as already described E2 and E3 genes were separately ligated into ... using primers that engineered restriction sites into the 5¢- and 3¢ ends of the gene products: NdeI and XhoI for the E2 gene, and NheI and EcoRI for E3 Oligonucleotides were as follows (restriction...

Ngày tải lên: 16/03/2014, 05:20

... functional and structural characterization of ETB and similar GPCRs Further analysis of the identified ETB131 and ETB93 fragments will help to identify residues involved in ligand binding and dimerization, ... digitonin, 0.4% Cloning procedures and protein analysis Coding regions of full-length ETB and its derivatives were amplified from cDNA by standard PCR techniques, and the fragments were inserted ... milk powder (Fluka, Buchs, Switzerland), and 0.1% (w ⁄ v) Triton X-100 Horseradish peroxidase-conjugated T7-tag antibody (Merck Biosciences) was diluted : 5000 and incubated for h with the membrane...

Ngày tải lên: 16/03/2014, 10:20

... Baldwin and K.S Nam 2010 Multiword Expressions Handbook of Natural Language Processing, Second Edition CRC Press, Taylor and Francis Group M -H Candito and B Crabb´ 2009 Improving gene erative statistical ... Computational Linguistics (COLING’86) J Lafferty and A McCallum and F Pereira 2001 Conditional random Fields: Probabilistic models for segmenting and labeling sequence data In Proceedings of the ... types of MWEs and the reference corpora are usually limited to certain types of MWEs Furthermore, the lexicon-driven and collocation-driven approaches not take the context into account, and therefore...

Ngày tải lên: 16/03/2014, 19:20

... expressed in the central and peripheral nervous systems, different isoforms are found in haematopoietic cells, osteoblasts, vascular endothelial cells and leukocytes, and are induced in various ... neuronal cells, and also in astrocytes and fibroblasts, acetylcholinesterase may have morpho-regulatory roles There, acetylcholinesterase may form a complex with amyloid precursor protein and perlecan ... 2008 FEBS L E Paraoanu and P G Layer Non-classical roles of acetylcholinesterase Fig Acetylcholinesterase as player in neurite growth and neural network formation in vivo and in vitro (Left) Formation...

Ngày tải lên: 23/03/2014, 07:20

... intact wild-type and ST mutant CPs can Fig Analysis of wild-type (UK3) PVX and ST mutant preparations by SDS/PAGE (8–20% gels) Lanes and 2, UK3 PVX; lanes and 4, ST mutant; lanes and 3, CPs from ... surface-located and glycosylated PVX CP N-terminal segment in preserving the structural and functional integrity of the PVX virions is discussed Materials and methods Reagents and chemicals Trypsin ... solution in a hydrated state, and the presence of carbohydrate residues may greatly increase the bound water content [20] To estimate bound water content in the intact and trypsin-treated PVX virions,...

Ngày tải lên: 23/03/2014, 13:20

... Vector Kit and BL21 (DE3) competent cells were purchased from Novagen (Madison, WI, USA) Prestained protein molecular mass standard was from Life Technologies (Grand Island, NY, USA) Firststrand cDNA ... SULT cDNA and a sites adaptor primer as the primer pair with the first-strand cDNA as the template Amplification conditions were at 94 °C and 25 cycles of 30 s at 94 °C, 30 s at 59 °C, and at 72 ... template and oligo(dT) as the primer, the first-strand cDNA was synthesized using the First-Strand cDNA Synthesis Kit (Amersham Biosciences) Using sense (5¢-CGCGGATCC ATGAGCCGGAGAACAAGCGAAACT-3¢) and...

Ngày tải lên: 23/03/2014, 15:20

... gastric mucosa and submucosa also showed ADH4 expression (data not shown) Fig Localization of ADH1 and ADH4 in stomach body, pyloric region and the gastroduodenal junction by ISH and IHC analyses ... gastric mucosa of stomach body (A) and region close to pylorus (B–D) ADH4 mRNA (E,F,I,J) and protein (G) in the cells lining gastric pits of mucosa in the stomach body (E,F) and the pyloric region ... absorption, distribution and metabolism of ethanol and its effects on nutrition and hepatic function In Medical and Social Aspects of Ethanol Abuse (Tabakoff, B., Sutker, P.B & Randall, C.L., eds),...

Ngày tải lên: 23/03/2014, 17:22

... between HSP90a and HtpG and between GRP94 and HtpG On the other hand, a complex was not formed between HSP90a and GRP94 as reported previously [22] Table Interaction between the middle and C-terminal ... domain, and client binding, could be separated into the N- and C-terminal parts, respectively However, the present study demonstrates that the two regions are unable to be separated and that ... Materials Expression vector pQE9 and plasmid pREP4 were purchased from Qiagen Inc (Chatsworth, CA, USA) and expression vector pGEX4T-1, glutathione-Sepharose and low-molecular-mass markers, from...

Ngày tải lên: 23/03/2014, 20:22

... system might generate the “stem and inflection” corresponding to and his brother Viewing and and his as inflection is problematic since a mapping from the English phrase and his brother to the Arabic ... for Computational Linguistics John Lafferty, Andrew McCallum, and Fernando Pereira 2001 Conditional random fields: Probabilistic models for segmenting and labeling sequence data In Proceedings of ... such as those of Avramidis and Koehn (2008), Yeniterzi and Oflazer (2010) and others Toutanova et al.’s work showed that it is most important to model target side coherence and our stem markup also...

Ngày tải lên: 24/03/2014, 03:20

... media and sufficient oxygen supply (Sandig et al., 2005)  Animal cells have low cell density and grow at slow kinetics (Sandig et al., 2005)  They are also high sensitive mechanical stress (Sandig ... acids, vitamin and salt (Xie et al., 2003) Serum is one of important components in cell culture media providing growth and adhesion factors, low molecular -weight nutrients, hormones, and growth factors ... transgene in a functional and nonrearranged form Continuous growth in culture, cell handling, and media manipulations could affect the stability of the inserted sequence (Wurm and Schiffmann, 1999)...

Ngày tải lên: 28/03/2014, 09:20

... fluorescence was monitored with excitation and emission wavelengths at 275 and 310 nm, with and nm bandwidth, respectively Buffer contributions were corrected and inner filter effect was negligible, ... Tyr81 and Arg46 are shown as a ball and stick model Surface of the cavities from ns-LTPmaize–palmitate complex (1mzm.pdb) (D), Par j (E) and Par j (F) models, unligated ns-LTPrice (1rzl.pdb) (G) and ... j and substituted by a lysine in Par j 2, whereas Tyr81 is absent in both Parietaria sequences The cavity of maize ns-LTP is highly polarized and mainly hydrophobic on one side, and polar and...

Ngày tải lên: 30/03/2014, 02:20