influence of resource quality on fungal activity
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 11 ppsx
... H, the rate of water diffusion is no longer influenced by adsorption b BSA Protonation BSA adsorption on montmorillonite leads to the protonation of the ionizable carboxylic groups of the protein, ... solution above pH (Fig 7) (42) b Preservation of the Secondary Structure on Adsorption Figure shows that the adsorption of α-chymotrypsin on montmorillonite has only a very small effect on the secondary ... Magnetic Resonance Detection of the Exchange of a Paramagnetic Cation on Protein Adsorption on Clays The principle of the method (16) is based on the fact that the adsorption of proteins on clays...
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Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf
... occupancies Superposition of subunit A on B and C (468 Ca atoms) in complex II and III of LlPDH gives an rmsd of ˚ 1.6 A in each case, however, superposition of B on C ˚ gives an rmsd of only 0.3 A We ... spite of conservation of key residues, may offer an explanation for those results Inhibition by PEX/PEA Well-defined electron density in the active site of complex III was modelled as a mixture of ... chain of Gln75, adopts a similar conformation, where NE2 donates a hydrogen bond to the adenine N7 and the nicotinamide ribose is hydrogen bonded to the carbonyl group of Val74 and amide of Ala76...
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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... formation of a d-galacturonate peak could be observed directly after addition of PelB, which is the result of its established galacturonase activity Only when high concentrations of PelB were used on ... the corresponding medium fraction was concentrated and similarly analysed Mode of action of PelB To examine its mode of action in more detail, hydrolysis products of oligogalacturonic acids generated ... homogalacturonan part of the pectin molecule Hydrolysis of PGA, analysed by highperformance size-exclusion chromatography (HPSEC), showed the initial formation of only monogalacturonic acid, with...
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Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... cells is a hallmark of CagT4SS activity Despite the molecular characterization of a number of effects in host cells, the exact function of CagA translocation during infection is still not fully ... be essential components of conjugation systems such as those of plasmids RP4 and F [31], and of the less related type IVB secretion systems, where the structure of a domain of the DotD lipoprotein ... interaction of secretion system components with b1 integrins is an important prerequisite for T4SS function Mechanisms of CagA recognition and transfer CagA as a type IV secretion substrate By contrast...
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Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... shift of the phosphate moiety from the aromatic purinetrione system to the periphery of the active site is restricted by the position of Arg128 from one side and the conformation of the loop connecting ... the position of the pyrimidine ring, whereas the phosphate and phosphonate moieties occupy the same position in spite of the different conformation of the alkyl chain The positions of the four ... K is the binding constant; n, number of sites; Mt, total concentration of macromolecule in V0; Xt and [X] are total and free concentrations of ligand, and Q is the fraction of sites occupied...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... Michaelis–Menten presentation, pH values are expressed as the corresponding proton concentrations (C) Apparent Km values of protons in the presence of proline (h) and L-alanine (s) as a function of the membrane ... Inhibition of control uptake 2.3 1.5 7.3 7.6 values taken from our previous studies [2,10] of praline, with 30% of maximal glycine currents at 20 mM concentration and a fairly high affinity of 0.25 ... as a function of external proton concentration, as shown for various voltage steps in Fig 5B After Eadie–Hofstee transformation, for each recorded membrane potential the apparent proton binding...
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Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales ppt
... detection of activity on ASC was developed The new system consists on the use of thin agarose gels supplemented with ASC, prepared on the surface of a glass slide As shown in Fig 2, activity of Cel48C ... incubation under these conditions The highest rate of hydrolysis was found on ASC (4.88 mUÆmg protein)1), reaching half of the maximum reaction velocity at a concentration of 0.21% ASC Activity ... solution One unit of enzyme activity was defined as the amount of enzyme producing lmol of p-nitrophenolÆmin)1 Activity at different pH and temperature was determined after incubation of the reaction...
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Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
... fractions, indicating the presence of a separate TatAy complex To confirm the association of TatAy and TatCy in a complex, the peak fractions from the first column were pooled and run on a second ... to a massive induction of PhoD synthesis A controversial aspect of studies into the TatAdCd system of B subtilis was the identification of a soluble substrate-binding species of TatAd in the cytoplasm ... derivative derivative containing containing containing containing containing the E coli tatABC operon; Ampr the B subtilis tatAdCd operon; Ampr the B subtilis tatAyCy operon; Ampr DmsA–GFP Ampr...
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Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... Purification of pantothenoic acid A solution of 20 mmol d(+)-calcium pantothenate in mL of m HCl was extracted once with 40 mL of chloroform ⁄ methanol (2 : 1) and three times with 40 mL of chloroform ... liganded conformation, it points into the tunnel, interacting with the N4 atom of the CoA substrate [2,10] His156 has previously been observed in only 6140 one conformation, having van der Waals contacts ... terminus of CoA is apparently in a somewhat strained conformation (Fig 3C) This indicates that the sulfur atom of Cys89 is crucial for the correct positioning of the reactive moiety of CoA In addition,...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
... the indirect dimension The mixing time used, 200 ms, was confirmed to have a negligible contribution from spin-diffusion Hydrogen bonding was Ó FEBS 2002 Mode of interaction of lytic peptides (Eur ... favored region AA, additional allowed region GA, generously allowed region ˚ DA, disallowed region None of the structures had NOE violation greater than 0.5 A and a dihedral angle violation greater ... Characterization of the tryptophan environment using fluorescence spectroscopy More information upon the membrane environment of the peptides was obtained by recording the fluorescence emission spectra of...
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Báo cáo khoa học: "Metaphor Comprehension - A special mode of language pptx
... The main conclusion of the paper is that the notions of processing capacity, memory constraints and control structure are the most salient constructs in sLmulation metaphor comprehension Recursive ... goal of the context construction procedure is to provide a context information in which the GCS-TKS connections are fully interpreted, that is, more fully integrated The paper discusses the conditions ... In addition, certain input metaphors require other comprehension strategies to be invoked, such as context construction or recursive metaphor interpretation COMPARISON WITH OTHER THE?RIES...
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Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... Dependence of the overall IN catalytic activity on its concentration effects of IN-interacting peptides An exponential autocatalytic effect, namely an exponential dependency of IN activity on its concentration ... Zeltser I, Gilon C & Kessler H (1994) Comparison of the conformation of active and nonactive backbone cyclic analogs of substance P as a tool to elucidate features of the bioactive conformation: NMR ... was continuously monitored under 485-nm excitation and 535-nm emission Enzyme-free control wells were subjected to the same reaction conditions but without IN in the reaction mixture, to monitor...
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Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... obstruction of stem–loop formation prevents termination and leads to transcription of cistrons on the 3¢ side of termination sites The ability of the CSP to prevent doublestrand formation in RNA ... compilation ª 2007 FEBS 1269 DNA single-strand binding to the cold shock domain K E A Max et al solution data have only indicated the formation of complexes consisting of one protein and one ligand ... N3 atoms of T1 Asp25, Lys7 and Trp8 contact N3 and O2 of T5 by hydrogen bonds in a similar way; O4 of T5 is connected to the Asp25 backbone carbonyl group via a water-mediated interaction The side...
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Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
... effect of salt on the conformational transition from a random coil to an a-helix, the CD spectra were recorded as a function of the NaCl concentration from to 100 mm at a constant peptide concentration ... on the biological activity, peptide structure, and interaction with membranes One interesting finding of this study was that introduction of a Pro in the middle position of the sequences of nonselective ... absence of acrylamide, F is the fluorescence of the peptide in the presence of acrylamide, KSV is the Stern-Volmer quenching constant, and [Q] is the concentration of acrylamide All conditions were...
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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... Hiromi, K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions Evaluation of subsite affinities of exo-enzyme Biochem Biophys ... inversion of the anomer configuration in contrast to the barley enzymes Transglycosylating activity has been demonstrated for 1,3-b-glucanases performing hydrolysis with retention of anomeric configuration ... displays exo -activity in the hydrolysis of 1,3-b-oligoglucosides, the subsite theory of Hiromi [46,47] was employed for construction of a subsite map for the estimation of affinities and number of subsites...
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Car Ownership and Mode of Transport to Work in Ireland* potx
... discussion of the effect of footpaths and cycle lanes on choice of mode of transport to school in Florida 11 =1 =1 =1 =1 Higher professional Lower professional Non-manual Manual skilled higher professional ... effect of education on the car-public transport option, however) VI SUMMARY AND CONCLUSIONS Despite the limitations associated with using Census of Population data to examine modal choice decisions ... endogeneity of the car ownership decision by estimating a joint model of car ownership and mode of transport to work The 2006 Census of Population also contains detailed information on home and...
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Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
... antimicrobial activity is a result of its inability to cross bacterial membranes Mechanism of action of GE23077 on E coli RNAP In order to obtain some basic information on the mechanism of action of GE23077 ... reaction mixture before the start of transcription (induced by DNA addition), resulting in complete inhibition of RNA synthesis Conversely, the addition of Fig Effect of GE23077 and other agents on ... of GE23077, assessing its activity on different purified polymerases Second, its mode of action on whole bacteria, using permeabilized cells to confirm the specificity of RNA synthesis inhibition...
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Báo cáo Y học: Exploring the primary electron acceptor (QA)-site of the bacterial reaction center from Rhodobacter sphaeroides Binding mode of vitamin K derivatives pptx
... variation of the redox potential of the naphthoquinone compounds and a detailed knowledge of their binding properties are of critical importance for the investigation of electron transfer reactions ... described by Breton et al [39] with minor modifications The sample (% 10 lL) containing about 1.5 nmol reconstituted RC was placed onto the depression of a CaF2 window After addition of lL of the aqueous ... presence of DAD/sodium ascorbate, leading to a fast reduction of the primary donor after electron transfer to QA) show exclusively the absorption changes upon the – reduction of the primary quinone,...
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stenersen - chemical pesticides - mode of action and toxicology (crc, 2004)
... PESTICIDES MODE OF ACTION AND TOXICOLOGY CHEMICAL PESTICIDES MODE OF ACTION AND TOXICOLOGY Jørgen Stenersen CRC PR E S S Boca Raton London New York Washington, D.C Library of Congress Cataloging-in-Publication ... activator Their mode of action is based on more new principles Most of them are listed below, together with the year of registration Chapter one: Introduction Pesticide Mode of Action Imazapic Imazamox ... the compound even to a person with moderate knowledge of chemistry One or more national standardization organizations and the International Organization of Standardization approve standard names...
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