inflorescence carbohydrate metabolisms transportation stress cell cycle and regulation
Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
... PMLA induced a strain specific enhancement of cell growth and an equal (between strains) shortening of the cell cycle Materials and methods Strains and materials The following strains of P polycephalum ... 2A,C,E) and cell cycle duration between the third and fourth mitosis (Fig 2B,D,F) The ratio of cell sizes in the figures is expressed relative to control (mock ¼ water-injected) plasmodia and reflects ... which Ó FEBS 2004 Polymalate effects growth and cell cycle (Eur J Biochem 271) 3809 Fig Effects of polymalate injection on growth and duration of the cell cycle Variable amounts of PMLA (A–D) or poly(L-glutamate)...
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Báo cáo khoa học: "Malignant mixed Mullerian tumors of the uterus: histopathological evaluation of cell cycle and apoptotic regulatory proteins" ppt
... pathways including cell proliferation, cell adhesion, cell death and apoptosis [2] Cell survival and apoptotic regulatory proteins such as the Bcl-2 family of genes, PCNA, p16, p21, p27, and cyclin D1 ... standardized across all cases studied Cell cycle and apoptotic regulatory proteins were analyzed for statistical significance as possible prognostic indicators Two cell cycle proteins, Mcl-1 and ... various biological markers, including both cell cycle and apoptotic regulatory proteins The cell cycle proteins studied include p16, p21, p27, p53, Cyclin D1 and Ki67 As seen in Table 3, p16 was Kanthan...
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Functional cooperation between FACT and MCM is coordinated with cell cycle and differential complex formation pot
... complex to cell cycle progression and regulation Functional attributes such as origin association, DNA unwinding activities, and complex formation are intimately coordinated with cell cycle progression ... preparation, chromatographic settings, and fraction collection and processing were done essentially as reported previously [32] Cell culture and cell cycle analysis All HeLa cells were maintained in Dulbecco's ... manufacturer's instructions For monitoring cell cycle progression and immunostaining analysis, collection of HeLa cells at different stages of the cell cycle was achieved by the mitotic shake-off...
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Báo cáo y học: " Single cell studies of the cell cycle and some models" pps
... selected cells come from more than a very narrow region of the cycle Some of the variation can be reduced by a correction for asynchrony [5] but there is still cell- to -cell variation in cycle stage and ... the cell cycle Tetrahymena pyriformis has a long and distinguished history in the cell cycle with its early induction synchrony But in the 1960s there was a burst of studies on selected single cells ... length of wild-type and wee1 mutant Sweiczer etfission in cell Schizosaccharomyces pombe, after cells of Modes fhe al Modes of growth in cell length of wild-type and wee1 mutant cells of fhe fission...
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Báo cáo y học: "Metabolic cycle, cell cycle, and the finishing kick to Start" potx
... of cells Glycogen and trehalose G1 S Start Cell- cycle progression (b) cAMP Glycogen and trehalose Ethanol Dissolved oxygen Respiratory quotient Budding of cells G1 S,M Start G1 Start Cell- cycle ... throughout the cell cycle of spontaneously synchronized cells [6,7]: that is, cells synchronized by growth in limiting glucose and experiencing synchronous waves of storing and then burning carbohydrate ... spontaneously synchronized with respect to the cell cycle: all the cells in the culture align at the same place in the cell cycle and then progress through the cycle together, an effect first seen more...
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Regulation of DNA (cytosine 5) methyltransferase 1 in the cell cycle and its role(s) in doxorubicin mediated micronuclei formation
... RESULTS AND DISCUSSION Regulation of DNMT1 expression in the Cell Cycle 3.1.1 Inverse relationship between DNMT1 and p21WAF1 in the 63 cell cycle 3.1.1.1 3.1.1.2 DNMT1 expression in the cell cycle ... mammalian cells but the expression level is cell cycle- regulated The interplay between DNMT1 and other cellular proteins is important in regulating its activity at different stages of the cell cycle ... important role in the regulation of the expression of genes essential for DNA replication and cell cycle progression (DeGregori and Johnson, 2006; Trimarchi and Lees, 2002) In quiescent cells, E2F1 is...
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Báo cáo y học: " Human T-lymphotropic virus type-1 p30 alters cell cycle G2 regulation of T lymphocytes to enhance cell survival" pptx
... increase of cells in G2 phase of cell cycle in p30 Jurkat T-cells of independent experiments was calculated by dividing the number of cells in G2 phase of cell cycle in p30 Jurkat T-cells over ... genes involved in cell cycle and apoptosis control[25] To examine if p30 expression results in alteration of cell cycle, we infected Jurkat Tcells with a p30 expressing lentivirus and tested the ... through the cell cycle After release from arrest, cells were collected at indicated time points and stained with propidium iodide and monitored for their progression through the cell cycle by flow...
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Báo cáo y học: " Classic swine fever virus NS2 protein leads to the induction of cell cycle arrest at S-phase and endoplasmic reticulum stress" potx
... cell cycle replication Presently, no data exists on the subcellular localization of CSFV NS2 protein and its effects on cell growth and cell cycle progression The swine endothelial vascular cell ... approximately × 106 cells of the stable cell lines and control cells were trypsinised and collected Following two washes with PBS, cells were resuspended in 70% ethanol and fixed at 4°C for 18 h Cells were ... of CSFV NS2 protein and elucidate the effects and mechanisms of this protein on cell growth and cell cycle progression Here, we show that the expression of NS2 protein causes cell growth retardation...
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Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor
... for controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth 1999) These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... cell cycle machinery and the pRB and p53 tumor suppressor pathways The universality of the cell cycle in all eukaryotes strongly suggested that anticancer therapy aimed at regulators of the cell ... S-phase entry and cell cycle progression Overexpression of D- and E-type cyclins and Cdk4 is observed frequently in human tumors (see Sect 5), supporting the notion that these cell cycle regulators...
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Senescence and Cell Cycle Control
... in cultured mouse cells p19ARF is predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 265 In human cells undergoing replicative ... Senescence and Cell Cycle Control 263 induction by controlling p16INK4a (and p19ARF in MEFs), while the detail of the promoter regulation requires further investigations In mouse cells, p19ARF ... (1995) The role of Rb in cell cycle control Prog Cell Cycle Res 1:9–19 268 H Kiyokawa Hayflick L, Moorhead PS (1961) The serial cultivation of human diploid cell strains Exp Cell Res 25:585–621 Herbig...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
... most cell cycle responses While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth factor deprivation and ... p107 and p130, defined the retinoblastoma gene family and added to the complexity of cell cycle regulation This chapter will elaborate on the role of the retinoblastoma gene family in cell cycle regulation ... delayed cell cycle exit of double knockout cells may indicate that oncogenic signals can induce a bypass of differentiation more easily in cells that lack both Rb and p107 or Rb and p130 In cells...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... mutant cells have been analysed phase of the cell- division cycle Therefore, we tested, using light microscopy and after DAPI staining, whether the slow growth yields a cell- cycle phenotype Cells ... the cell cycle and highly resistant to proteolytic degradation [40] Miscellaneous procedures Yeast were grown on standard media [41] and transformed as described by Gietz et al [42] Microscopy and ... in cell- cycle progression Yeast cells deprived of RIO1 lose minichromosomes at an increased rate relative to wild-type and arrest either as large G1 cells (i.e late in the G1 phase of the cell- division...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... down-regulated upon cell cycle exit To this effect, HFF cells were deprived of serum for 48 h to induce cell cycle exit Serum was then re-added and samples taken as cells progressed into the cell cycle Total ... [13] When cells exit the cell cycle, licensing in lost, and is established again as cells prepare to reenter the cell cycle While a previous study did not detect a significant down -regulation ... conclude that Cdt1 and geminin mRNA levels are reduced in quiescent cells, suggesting that in contrast to their regulation during the cell cycle [13], upon exit and entry to the cell cycle both genes...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... removed, and cells were washed several times with normal medium and then grown under standard conditions For proteasome inhibition, HeLa cells were synchronized by a double thymidine-block and released ... phases of the cell cycle, HeLa cells were arrested by a double-thymidine procedure at the G1 phase/S phase transition, and then released into the cycle after removing excess thymidine Cells were ... Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase Mol Cell Biol 18, 2758–2767 26 Fujita, M (1999) Cell cycle regulation of DNA replication...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... proteins in cell cycle regulation Similarly, independent studies have shown that the MLLinteracting proteins menin, host cell factor (HCF1) and CGBP are also implicated in cell cycle regulation ... in cell cycle checkpoint regulation, probably via the involvement of p53 and p21 MLL1 and H3K4 methylation are critical for Hox gene regulation during the cell cycle In order to understand the ... the cell cycle As MLLs and H3K4-specific methylations are well known to play critical roles in gene expression, we analyzed the dynamics and functions of MLLs and H3K4 methylation during cell cycle...
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Báo cáo khoa học: The inhibition of Ras farnesylation leads to an increase in p27Kip1 and G1 cell cycle arrest pdf
... p27 downregulation and S phase entry in fibroblasts and epithelial cells Am J Physiol 277, C652–C664 30 Slingerland, J & Pagano, M (2000) Regulation of the cdk inhibitor p27 and its deregulation ... arrest of Rat1/ras cells at the G1 phase of the cell cycle We next examined the effect of HR12 on the distribution of Rat1/ras cells in the cell cycle To resolve the G1, S and G2/M phases, we ... Rat1/ras cells, and whether the timing of Ras-processing and pRb phosphorylation are correlated We treated Rat1/ras cells with 20 lM HR12 in growth medium and lysed the treated and untreated cells...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... and Fission Yeasts Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce Summary The cell cycles of the budding yeast Saccharomyces cerevisiae and ... (MluI -cell cycle box) elements and is composed of Mbp1 and Swi6 MBF binding is cell cycle- regulated (reviewed in ref 12) Targets of MBF and SBF include cyclins, cell wall biosynthesis genes, and ... The Plant Cell Cycle 43 Modified Plant Cell Cycles Subheading described the typical cell cycle as observed in most meristematic and culture cells, but a number of variant types of cycle are found...
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cell cycle checkpoints and cancer
... 1-58706-067-1 (alk paper) Cancer cells Regulation Cell cycle Cellular signal transduction Cell transformation Carcinogenesis [DNLM: Cell Transformation, Neoplastic Cell Cycle QZ 202 C39253 2001] I ... 33 Cytokine Regulation of Cell Cycle Progression 34 Links Between the Ras/Raf/MEK/ERK Pathway and Cell Cycle Proteins 34 Cytokine Regulation of Apoptosis and Cell Death 34 ... UNIT 15 Cell Cycle Checkpoints and Cancer Mikhail V Blagosklonny Bethesda, Maryland, U.S.A LANDES BIOSCIENCE GEORGETOWN, TEXAS U.S.A EUREKAH.COM AUSTIN, TEXAS U.S.A CELL CYCLE CHECKPOINTS AND CANCER...
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Cell Metabolism Cell Homeostasis and Stress Response Part 15 doc
... therapy: oncogene and nononcogene addiction Cell 136: 823-837 Manders, E.M.M., Kimura H & Cook P.R 1999 Direct imaging of DNA in living cells reveals the dynamics of chromosome formation J Cell Biol ... components of gene expression machinery into daughter nuclei Mol Biol Cell 14: 1043-1057 206 Cell Metabolism – Cell Homeostasis and Stress Response Prasanth, K.V., Prasanth S.G., Xuan Z., Hearn S., ... the kinetic of post-mitotic transcriptional re-activation Nat Cell Biol 13: 1295-1304 208 Cell Metabolism – Cell Homeostasis and Stress Response Zitzmann-Kolbe, S., Strube A., Frisk A.L., Kakonen...
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Cell Metabolism Cell Homeostasis and Stress Response Part 1 pdf
... out by oligosaccharides, and particularly, by oligoglucans 2 Cell Metabolism – Cell Homeostasis and Stress Response Oxidative stress and reactive oxygen species Oxidative stress is defined as the ... chloroplasmatic APX (in the stroma and thylakoid) (Ishikawa et al., 1996) and mitochondrial (De Leonardis et al., 2000) Each one, Cell Metabolism – Cell Homeostasis and Stress Response with a specific ... Fabiana Zanelato Bertolde and Alex-Alan Furtado de Almeida Chapter Oxygen Metabolism in Chloroplast 39 Boris Ivanov, Marina Kozuleva and Maria Mubarakshina Chapter Stress and Cell Death in Yeast Induced...
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