induction plant regeneration and genomic dna extraction
báo cáo khoa học: " Tissue culture-induced transpositional activity of mPing is correlated with cytosine methylation in rice" ppsx
... containing an immobile mPing copy and one containing an active one further bolstered this possibility Methods Callus induction, plant regeneration, and genomic DNA extraction Embryos from mature ... types of bands were resolvable: (i) monomorphic bands uniformly present in the donor plant and its corresponding calli and regenerants, (ii) polymorphic bands present in the donor plant but disappeared ... plant (V27Reg5) of cv V27, determined by genomic bilsulfite in seed -plant (ITDTG8) (a) and an maps and collective methylation locus (in percentage) for an inactive (immobile) calli (V27Ca2) and...
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Báo cáo khoa học: "Making Lexical Ontologies Functional and Context-Sensitive" pdf
... between nouns and their adjectival forms The function (%sim arg0 CAT) reflects the perceived similarity between the putative member arg0 and a synset CAT in WordNet, using one of the standard formulations ... %sim in the functions of Figures 2, and means that these membership functions readily admit both literal and metaphoric members Since the line between literal and metaphoric uses of a category ... the middle-layer of WordNet, such as Person, Substance, Animal, Tool, Plant, Structure, Event, Vehicle, Idea and Place, and by pre-fetching the query ”C-like H” for all 4061 nouns collected in...
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Báo cáo khoa học: "A Freely Available Morphological Analyzer, Disambiguator and Context Sensitive Lemmatizer for German" pdf
... possible prefixes and suffixes and reverses the process of vowel mutation if umlauts are found (shifts between B and ss are treated analogously) Each root is looked up in the lexicon, and - if found ... tagging and context sensitive lemmatization was introduced The morphological analyzer is based on the standard Duden grammar and provides wide coverage due to a lexicon o f 324,000 word forms and ... dust corners); and the linking letters e and s are not always determined phonologically, but in some cases simply occur by convention (e.g Schwein-e-bauch but Schwein-s-blase and Schwein-kram)...
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Báo cáo "Design of phase-coded transmitter and high sensitive receiver of radar system " doc
... electronic components and measure characteristics Fig.5 is a photo of experimental amplifier The first and second stages have output power of 45 W and 90 W, corresponding, of a standard input 300 ... linearity and large dynamic band enables the amplifier of ECC-01 can receive UHF signals from -30dBm to -108dBm Furthermore, a Timing Automatic Gain Controller (TAGC) is used to enhance dynamic band ... unwieldy and needed so many logic gates with uncontrolled of delay time and bits properties In our method, the microcontroller PIC16F877A has been selected The circuit is very simple and with...
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Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors potx
... phenanthroline/Fe2+ complex, and then quantifying the concentration of a known dilution spectrophotometrically [14] Cell lines and flow cytometry MCF7, BT-474, and MDA-MB-231 breast cancer cells and Chinese hamster ... streptomycin (vol/vol), and μg/mL ciprofloxacin MCF7/Her2-18 and parental CHO cells were cultured in an incubator at 37°C with 5% CO2 and maintained at a cell concentration of between × 105 and × 10 viable ... National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee Results and discussion Characterization of nanoparticles...
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Radiological Findings of Extensively Drug-Resistant Pulmonary Tuberculosis in Non-AIDS Adults: Comparisons with Findings of Multidrug-Resistant and Drug-Sensitive Tuberculosis docx
... Radiographic and CT Findings Reviews of chest radiographs and CT scans were performed at least two months apart for concern of recall bias Chest radiographs and CT scans were reviewed in a random order ... Intervals between TB isolation and a chest radiograph were significantly longer in XDR TB patients than DS TB patients and MDR TB patients (p = 0.001 for a chest radiograph and p = 0.024 for CT) Imaging ... scanning parameters were 120 kVp and 90-170 mA Scanning and image reconstruction for single-detector CT was performed with a section thickness of mm, a pitch of and a reconstruction interval of...
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RFID reader detector and tilt sensitive RFID tag
... antenna and connected chip http://www.instructables.com/id/RFID_Reader_Detector _and_ Tilt_Sensitive_RFID_Tag/ step 1: Material and Tools This section provides an overview of the necessary materials and ... 1003207at www.farnell.com) Tools (see Figure and 3): - Craft knife and scissors - Insulating tape (e.g., order number 1373979 at www.farnell.com) - Soldering iron and solder RFID reader for testing (see ... of the tag antenna, and therefore also variable reading distance of the tag (Figure 1) - Experiments with the tag size and material (Figure 2) - Switching between the LED and an RFID chip (Figure...
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báo cáo khoa học: "Enzymatic- and temperature-sensitive controlled release of ultrasmall superparamagnetic iron oxides (USPIOs)" pdf
... a PEG block and then a biofunctional ligand (e.g., peptides, amine-functionalized ssDNA) in modular fashion, yielding PEG-PPS-based polymer-biomolecule conjugates spectroscopies, and further ... micelles deposited and dried on the same copper TEM grids out of water (Figure 3b) The addition of PPS-PEG-ssDNA conjugates into the micellization process immobilized ssDNA on the USPIOs and also produced ... USPIO clusters were being subjected to heating and cooling In this process, heating and cooling of the clusters melts and reanneals the crosslinking DNA sequences, respectively The resulting changes...
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báo cáo khoa học: " Comparison of Radioimmuno and Carbon Nanotube Field-Effect Transistor Assays for Measuring Insulin-Like Growth Factor-1 in a Preclinical Model of Human Breast Cancer" doc
... Baltimore animal facility and maintained in accordance with institutional guidelines approved by the University of Maryland, Baltimore Animal Care and Use Committee To compare RIA and CNT-FET assays, ... MMTV-Cre mice and wild type C57Bl/6 mice were euthanized at 3, and 12 months of age to collect trunk blood For histology, mammary tissue from 3, and 12 month old mice was removed post mortem and formalin ... September 2011 References Hadsell DL, Bonnette SG: IGF and insulin action in the mammary gland: lessons from transgenic and knockout models J Mammary Gland Biol Neoplasia 2000, 5:19-30 Rinaldi S, Toniolo...
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Báo cáo khoa hoc:" Correction: Enzymatic- and temperature-sensitive controlled release of ultrasmall superparamagnetic iron oxides (USPIOs)" potx
... Hubbell4 and Todd D Giorgio1,2* Affiliations: Department of Biomedical Engineering, Vanderbilt University; Nashville, Tennessee, USA Vanderbilt Institute for Nanoscale Science and Engineering, Vanderbilt ... Vanderbilt University; Nashville, Tennessee, USA Integrative Biosciences Institute, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland Email: SSY : shann.yu@vanderbilt.edu RLS: randy.l.scherer@vanderbilt.edu ... randy.l.scherer@vanderbilt.edu RAO: ryan.a.ortega@vanderbilt.edu CSB: charleson.bell@vanderbilt.edu CPO'N: conlin.oneil@epfl.ch JAH: jeffrey.hubbell@epfl.ch TDG: todd.d.giorgio@vanderbilt.edu *Corresponding...
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Báo cáo y học: "Science review: Redox and oxygen-sensitive transcription factors in the regulation of oxidant-mediated lung injury: κ role for nuclear factor-κ" pdf
... Hetero-Chromatization And Fragmentation Cytoplasmic Swelling Mitochondrial Dysfunction Membrane Rupturing Membrane Blebbing And Apoptotic Bodies Random and Violent Death Cell Suicide And Dismantling ... activation of NF-κB and the signal transducer and activator of transcription, and that increases in IL-6 and granulocyte colony-stimulating factor mRNA levels in the lungs and livers measured ... financially supported by the Anonymous Trust (Scotland), the National Institute for Biological Standards and Control (England), the Tenovus Trust (Scotland), the UK Medical Research Council (MRC, London),...
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Báo cáo y học: "Science review: Redox and oxygen-sensitive transcription factors in the regulation of oxidant-mediated lung injury: α role for hypoxia-inducible factor-1α" pptx
... that of NF-κB Indirect, but unprecedented and unequivocal, evidence was independently provided by Hellwig-Bürgel and colleagues [47–49] and by Haddad and Land [50,51], however, to indicate HIF-1 ... financially supported by the Anonymous Trust (Scotland), the National Institute for Biological Standards and Control (England), the Tenovus Trust (Scotland), the UK Medical Research Council (MRC, London), ... antibodies enhanced serum levels of TNF-α and macrophage inflammatory protein-2, and it increased hepatic neutrophil accumulation and the amount of liver injury and lung injury [7–13] These data indicate...
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Báo cáo y học: " Human TRIM5α mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants Patrick Kaumanns, Isabel Hagmann and Matthias T " pps
... from PD Bieniasz, [21]) and G418 resistant cells were selected TZM-bl cells are HeLa-cell derivatives that express high levels of CD4 and both co-receptors CXCR4 and CCR5, and are stably transduced ... Challenging these indicator cells with HIV-1 and HIV-2 isolates results in the induction of luciferase and β-galactosidase allowing easy detection of infection and titration In the absence of an antibody ... interests 14 Authors' contributions PK and MTD conceived the experiments and wrote the manuscript PK, IH and MTD performed the laboratory work All authors read and approved the final manuscript 15...
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Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids
... turn improves the assay sensitivity, and (III) a single-strand DNA (ssDNA) standard bearing the same sequence and length as of the target gene was created and accurately quantified for use in ... cultures and mRNA extraction 184 6.2.4 Preparation of sense p185-ssDNA 184 6.2.5 bDNA hybridization assay 184 6.3 Results and Discussion 185 6.3.1 Detection and signal ... of the biotinylated double-strand DNA (dsDNA) .131 4.2.6.2 Purification of biotinylated dsDNA and gel electrophoresis .132 4.2.6.3 Purification of sense p185-ssDNA 133 4.2.7 Real-time...
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Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays" pptx
... for DNA methylation analysis: COBRA, bisulfite SNuPE, bisulfite pyrosequencing, MethyLight, MSP and MeDIP-qPCR The first five methods utilize bisulfite treatment of genomic DNA to detect DNA ... performance ranking of DNA methylation assays and candidate biomarkers (Additional data file 1); the XML-based PMML model that MethMarker uses for exporting, importing and storing candidate biomarkers ... design, optimization and computational validation of candidate biomarker DNA methylation assay for the MGMT promoter, two key steps remain: experimental assay validation and experimental biomarker...
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Báo cáo y học: "ptimized design and data analysis of tag-based cytosine methylation assays" pps
... The adapter and primer sequences for library preparation are listed in Table S9 in Additional file Genomic DNA (5 μg) was digested with HpaII and MspI in separate 200 μl reactions and purified ... contributions MS and JMG designed the assays and strategies for its analysis, MS performed all library preparation and characterization, MS, DL and MP performed bisulfite validation studies, while QJ and ... strengths of MSCC and HELP-seq/Methylseq, and the supporting analytical workflow that maximizes the quantitative capabilities of the data generated Results and discussion Library preparation and sequencing...
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Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
... originated from ear tissue explants Genomic DNA was extracted from leukocytes and fibroblasts using a commercial DNA extraction kit (QIAamp ® DNA mini kit, Qiagen, Hilden, Germany) DNA from sperm cells ... G-L = Genomic leukocytic DNA, G-F = Genomic fibroblastic DNA, D-S = Digested sperm DNA, DL = Digested leukocytic DNA, D-F = Digested fibroblastic DNA Phutikanit et al Acta Veterinaria Scandinavica ... clear and reproducible marker bands were counted, and the comparison of bands between genomic and digested templates was done according to the appearance of the marker mentioned above Number and...
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Báo cáo y học: "Translational Medicine and Reliability of Single-Nucleotide Polymorphism Studies: Can We Believe in SNP Reports or Not"
... polymorphisms and cancer outcome (mainly colorectal, breast, and lung malignancy) SNPs for GST polymorphisms were used as a substrate for analyses Materials and Methods Search strategy and eligibility ... enthusiastic about projects that have positive results and are more likely to complete them and submit them for publication [4] On the other hand, authors of studies with negative results are disappointed ... (GSTM1 and GSTT1) and lung cancer outcome Cancer Invest 2006;24:497-501 498 24 Goto I, Yoneda S, Yamamoto M, Kawajiri K Prognostic significance of germ line polymorphisms of the CYP1A1 and glutathione...
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Báo cáo y học: "Association between regulated upon activation, normal T cells expressed and secreted (RANTES) -28C/G polymorphism and asthma risk – A Meta-Analysis"
... Table and Figure 1) We further performed stratified analysis according to asthma type (atopic asthma and non-atopic asthma), age (children and adult) and ethnicity (Asian and Caucasian and African ... heterogeneity, and it was considered significant for P < 0.01 We used the fixed-effects model and the random-effects model based on the Mantel-Haenszel method and the DerSimonian and Laird method, ... cases and 1766 controls for -28C/G Materials and Methods Identification and Eligibility of Relevant Studies Eligible studies were identified by searching the electronic literature PubMed and EMBASE...
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Báo cáo y học: "Grb2-associated binder 1 polymorphism was associated with the risk of Helicobactor pylori infection and gastric atrophy"
... AA, and R2: 5’ CTG GGG AGT GGG CCA Genomic DNA was applied in a volume of 25 ul with 0.12 mM dNTPs, 25 pmol of each primer, 0.5 units of AmpliTaq Gold (Perkin-Elmer Corp., Foster City, CA), and ... cases and controls with seropositive atrophy The ORs of the G/A and A/A for gastric cancer was 1.05 (95% CI 0.63-1.74) and 1.05 (95% CI 0.27-4.07), respectively Table The Gab1 genotype frequency and ... cytokine and growth factor receptors and T- and B-cell antigen receptors Blood 1999; 93: 1809-1816 23 Hamajima N, Matsuo K, Saito T, et al Interleukin polymorphisms, lifestyle factors, and Helicobacter...
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