incidence of violative residues in foods

... by ORFs coding for putative proteins of uncharacterized activity and specificity A striking feature of GH-57 is the sequence and length diversity of the individual members Indeed, certain GH-57 ... SLH-like domain (SLD2), a threonine-rich region and a putative transmembrane domain [22] Interestingly, the 3D structure of a protein domain, which is clearly homologous to SLD1 and -2 of Q9Y8I8_THEHY, ... 4) Importantly, the overall integrity of all subfamilies was saved in all trees, including the Pfam-tree, based on simplified alignment of  300 N-terminal amino acid residues Therefore, together...

Ngày tải lên: 19/02/2014, 12:20

... mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme To obtain an insight into how the many ... formation of the oxazolinium ion intermediate; (C) Hydrolysis of the oxazolinium ion intermediate Copyright National Academy of Sciences, USA questioned on the basis of studies of chitinase A from ... software [41] Catalysis in family 18 chitinases (Eur J Biochem 271) 255 Results and discussion Figure shows part of the structure of ChiB, highlighting the residues discussed in this report In...

Ngày tải lên: 07/03/2014, 14:20

... pulses of 30 s at 30-s intervals, at 50 W in 10 mL of 50 mM Tris/HCl, pH 8.0, containing 0.5 M NaCl, 0.1% (v/v) 2-mercaptoethanol, and protease inhibitors (2 lgÆmL)1 aprotinin, lgÆmL)1 pepstatin ... primarily in inclusion bodies Successive washes of inclusion bodies with M urea and 5% (v/v) Triton X-100 removed most of the contaminating proteins, producing a Fig SDS/PAGE analysis of the expression ... added to the incubation media In both cases time-dependent inactivation was observed, which is indicative of irreversible binding to one/several of the sulfhydryl moieties involved in the enzymatic...

Ngày tải lên: 08/03/2014, 08:20

... drugs includes lincomycin, clindamycin, and pirlimycin; two of these drugs—lincomycin and pirlimycin—are approved for use in food-producing species Lincosamides are derivatives of an amino acid ... used in pigs to treat joint infections and pneumonia Several combination products containing lincomycin are approved for use in food-producing species A lincomycin–spectinomycin product administered ... pirlimycin is an analog of clindamycin The lincosamides inhibit protein synthesis in susceptible bacteria by binding to the 50S subunits of bacterial ribosomes and inhibiting peptidyltransferases; interference...

Ngày tải lên: 11/03/2014, 03:20

... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of ... or heterodimers of subunits ranging from 23 to 30 kDa in size Subunits of all known GST structures exhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly ... arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH-binding or cysteine-binding proteins The H-site is entirely helical, with a variable number of a-helices,...

Ngày tải lên: 15/03/2014, 09:20

... bioconversion of chitin into valuable oligosaccharide derivatives for various industrial applications Pf-ChiA has a unique multidomain structure containing two chitin-binding domains (ChBD1 and ... number of amino acid residues contribute to the binding of the substrate by hydrogen bonding and ⁄ or hydrophobic interactions Using the ligplot program [31], we investigated the specific interactions ... degree of sequence similarity The catalytic domain of family 19 chitinases comprises two lobes, each of which is rich in a-helical structure [16,17] In contrast, family 18 includes chitinases...

Ngày tải lên: 15/03/2014, 11:20

... situated in the interface between the helicase and the protease domains, creating a well-dened binding cleft (Fig 1A) [7] Indeed, it appears that certain residues in the helicase domain may interact ... enzyme, the C-terminus is located in the substrate binding site and the helicase clearly interacts with the C-terminus of NS3 (Fig 1B) [7] By structural modelling of an inhibitor binding to full-length ... level Inhibition of NS3 protease variants The effect of changes in the helicase residues on the inhibition of the protease was investigated for the three variants and a series of inhibitors Inhibition...

Ngày tải lên: 16/03/2014, 06:20

... results in this paper suggest that point mutations to C-terminal residues (per- Ó FEBS 2002 Role of arginine residues in DAOCS (Eur J Biochem 269) 2739 haps in conjunction with C-terminal truncation ... coupling of 2-oxoglutarate conversion to prime substrate oxidation In the case of DAOCS it has been previously shown that mutations to residues involved in 2-oxoglutarate or penicillin binding ... stimulation of 2-oxoglutarate conversion was observed in the presence of penicillin G for the R160Q, R162Q and R162A mutants (Table 2, entries 10–12), consistent with loss of penicillin binding Arginines...

Ngày tải lên: 18/03/2014, 01:20

... important in the literature and have roles assigned [12–18,20]: *, CTP-binding residue of the P-loop; (*), CTP-binding residue; ^, Neu5Ac-binding residue; #, Neu5Ac-binding residue forming part of the ... pocket plays a role in substrate discrimination against binding of the 5-OH group of Kdo and in favour of the N-acetyl group of Neu5Ac and, although these residues are conserved in CNS enzymes, none ... significant increase in Km(Neu5Ac) would support a role in binding that substrate In order to investigate this further we made a series of mutations at Gln104 We maintained the length of the side chain...

Ngày tải lên: 22/03/2014, 21:21

... modeling-based mutagenesis of the TM domains of the prostanoid receptors suggested that the conserved regions of the third and seventh TM domains are involved in binding the common structures of ... western blot as described in (Aa) The position of IP is indicated on the left by an arrow (b) Binding of [3H]iloprost to recombinant IP expressed in HEK293 cells The binding of the IP agonist to the ... [3H]iloprost-binding activity (Fig 4) Very little binding activity was observed for each of the three mutants in the assay, even when increasing amounts of [3H]iloprost were used A combination of the...

Ngày tải lên: 23/03/2014, 07:20

... phosphotyrosine Diethyl pyrocarbonate is the most frequently used histidine modifying reagent preventing subsequent phosphorylation of histidine residues Screening for proteins containing N-linked ... serine, threonine and tyrosine residues, thrombin and collagen lead to the phosphorylation of histidine in the cytoplasmic tail of P-selectin The appearance and disappearance of phosphohistidine ... 2A and type 2C, in contrast, are highly active against phosphohistidine in histone H4 (reviewed in [1]) Intriguingly, none of the serine/threonine phosphatases acting on histidine phosphorylated...

Ngày tải lên: 24/03/2014, 03:21

... These findings are intriguing given that these three cysteine residues are absolutely conserved not only in the F proteins of other Pneumovirinae, but also in the F proteins of the Paramyxovirinae ... Diagram 1of the HRSV F protein Diagram of the HRSV F protein A linear representation of the HRSV F precursor protein (A2 strain) is shown Amino acid positions of individual domains are indicated ... cysteine residues in the extracellular domain in protein expression and cell fusion of the HRSV F protein and represents the first analysis of the contribution of the cysteine residues of the...

Ngày tải lên: 20/06/2014, 01:20

... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of ... or heterodimers of subunits ranging from 23 to 30 kDa in size Subunits of all known GST structures exhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly ... arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH-binding or cysteine-binding proteins The H-site is entirely helical, with a variable number of a-helices,...

Ngày tải lên: 07/08/2014, 01:20

... purpose of the study reported in this paper was to define the contribution of different CDRs, and of individual arginine residues within those CDRs, in binding to CL Patterns of CDR arginine residues ... CL The effect of point mutations of specific arginine residues in CDR3 of IS4VH upon CL binding is shown in Fig The low binding of IS4VH/IS4VL was abolished by inclusion of any one of these mutations ... numbers of arginine residues in the CDRs of these antibodies can lead to significant alterations in binding to DNA Arginines in VH CDR3 often play a particularly important role in binding to this...

Ngày tải lên: 09/08/2014, 06:22

... estimation of their global incidence [1] Although collected in international databases, most case reports are published by clinicians from industrialized nations Little is known about the incidence of ... Editor -in- Chief of this journal In the case of patient who died, consent for publication was sought from his next of kin Competing interests The authors declare that they have no competing interests ... acetic acid intoxication During binge drinking, a 42-year-old Asian man, a chronic alcoholic, involuntarily ingested ~100 ml of 80% acetic acid After hospital admission, he developed intravascular...

Ngày tải lên: 11/08/2014, 19:21

... estimated incidence of severe sepsis in 0.61% of all hospital admissions and 11% in all ICU admissions [15] Annual incidence based on the incidence series of cases A second estimate of the annual incidence ... in numbers of persons, Ij is the incidence rate in persons (new cases)/time, and Ei(D) is the expected value of the duration of the condition in a distribution of durations obtained from an incidence ... 2003, 348:1546-1554 Ibelings MS, Bruining HA: Dutch results of the European Study of Prevalence of Infection during Intensive Care (EPIIC) II Nature of the infections [in Dutch] Ned Tijdschr Geneeskd...

Ngày tải lên: 12/08/2014, 20:20

... started in 2008 with 14.9 incidence rate per 100,000 population with the highest incidence rate of 29.2 per 100,000 population reported in 2010 (Kondakci and Yuk, 2012) This alarming increase in incidence ... result in level of target pathogens not reaching the limit of detection level due to the low numbers of target pathogens present, the presence of background microflora competing for nutrients or insufficient ... materials of construction that not support cell viability in food processing plants and to maintain plant hygiene at all times to minimize cross contamination due to contact or handling vii LIST OF...

Ngày tải lên: 30/09/2015, 10:11

... Quantity of dioxin contained in herbicides: - Folkin: 500 kg - Stellman: 366 kg (≈ 240 times total amount of dioxin/furan in USA year 2000) -Vietnam-Russia Tropical Center: 1000 kg Dioxin contamination ... sampling, analysing to determine dioxin concentration in these airports For Danang airport, it have been identified some more contamination places at the South of aiport; in Bienhoa airport also indentified ... Spills of Herbicides in Phu Cat Airbase  Spills and leakages occurred due to handling (loading and washing) Phu Cat: Area contaminated Dioxin, Volume to be Treated Location Area (m2) Loading Area...

Ngày tải lên: 23/06/2016, 00:19

... in remaining particles Furthermore, the degree of homogeneity achieved is generally not as good as in cryogenic processing leading to greater sub-sampling variations If the nesessary degree of ... During validation each laboratory should investigate the typical sub-sampling variabilities achieved when employing the available comminution devices, using representative samples containing incurred ... existing regulatrions, directives or guidelines In the case of fruits and vegetables, cryogenic milling (e.g using dry ice) is highly recommended to increase homogeneity and thus reduce sub-sampling...

Ngày tải lên: 06/10/2016, 09:38

... control of quarter samples Nordic Veterinarian-Medicine, 1974, 26, 197-204 Minitab statistical software For windows 2000 Mintab Inc USA, 2000 10 National Mastitis Council Laboratory Handbook on Bovine ... antimicrobial residues 18 PhD Thesis, University of Nairobi 1997 13 Osteras O, Edge V, Martin S Determinants of success or failure in the elimination of major mastitis pathogens in selective dry ... measures of milk quality and risk of violative antimicrobial residues There are however many factors that contribute to the overuse of antimicrobials in farming which arises from a complex interaction...

Ngày tải lên: 07/08/2014, 17:22