in vivo imaging approaches of nitric oxide with multimodal imaging

... production of NO in macrophages stimulated with LPS, via activation of NF-jB and its binding to region in the promoter of the iNOS gene [11,12] In the present study, LPS-induced NO production in the ... secreted protein, in murine macrophages upon LPS stimulation [22] This mechanism is, however, hard to consider as the main underlying mechanism of LPS-induced IFN-c production in vivo, in view of the ... factor IRF-1 in NO synthase induction in macrophages Science 263, 1612–1615 14 Martin, E., Nathan, C & Xie, Q.-W (1994) Role of interferon regulatory factor in induction of nitric oxide synthase...

Ngày tải lên: 17/03/2014, 10:20

... cleavage of inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in ... higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following HSD treatment To promote in vivo calpain activation, ... M Averna et al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material...

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 07/08/2014, 15:20

... possible An alternative mode of imaging is the use of single photon imaging of γ-emitting isotopes like 11 1In or 99Tc Imaging of γ-emitting isotopes is referred to as single photon emission commuted ... rheumatologists interested in surgical procedures for in vivo imaging, there is a rich arsenal of procedures for imaging within almost all major organs of mice or rats Almost all were developed originally ... be collected in a couple of minutes of recording, the migration of leukocytes in tissues is relatively slow and requires many minutes of recording to track cells This longer imaging period requires...

Ngày tải lên: 09/08/2014, 01:22

... sonicated in a bath-type sonicator for 5min The amount of protein of the solubilized cells was determined with a bicinchoninic acid protein assay kit (PIERCE, Rockford, IL, USA) using bovine serum ... described in Fig In this study, to obtain information about the mode of actions of TS, we examined the effect of TS on LPS/IFNinduced NO production in VSMC We found that TS activated LPS/IFN-induced ... level of NO production inhibited with PKC inhibitor Ro31-2880 was almost the same as that of LPS/IFN-induced NO production without TS (Fig 5A), suggesting that the enhancement of NO production with...

Ngày tải lên: 22/02/2014, 04:20

... combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of ... cytotoxic and DNA-damaging effects of nitric oxide [29] So, while the binding of NO to GSH in vivo does not obviously prevent NO from interacting with MT2, we have shown that in vitro it suppresses ... suppression of the expression and activity of inducible nitric oxide synthase (iNOS) by zinc [32,33], released from MT under the in uence of nitric oxide (NO), and the scavenging of NO through...

Ngày tải lên: 07/03/2014, 15:20

... TNFα in the presence A: absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with increasing concentrations of ... forebrain cholinergic neurons [57] and direct TNFα administration in the brain of mice results in an impairment of basal forebrain cholinergic function [58] However, whether induction of iNOS and ... cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells Diabetologia 1998, 41:1101-8 Kong GY, Peng ZC, Costanzo C, Kristensson K, Bentivoglio M: Inducible nitric oxide...

Ngày tải lên: 19/06/2014, 22:20

... expression of both nNOS and eNOS in the spinal cords of rats with EAE, small amounts of iNOS were identified in the normal spinal cords but its expression slightly increased in the spinal cord of 5CFAtreated ... (Fig 3, graph), iNOS immunoreactivity in the spinal cord of rats with EAE significantly increased compared with that in the spinal cord of normal rats The increased expression of iNOS persisted ... localization of nNOS, eNOS, and iNOS in EAE In the spinal cords of rats with EAE, the expression of nNOS was found in some small neurons and in the spinal cord parenchyma with a granular pattern In addition,...

Ngày tải lên: 07/08/2014, 14:22

... and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximal staining ... patterns of eNOS immunostaining in the ovary were largely consistent with those of previous reports [11,12] Although the expression of iNOS in porcine ovaries is well known, our findings contrast in ... The involvement of nitric oxide in the ovulatory process in the rat Endocrinology 1994, 135, 2287-2290 11 Takesue K, Tabata S, Sato F, Hattori MA Expression of nitric oxide synthase-3 in porcine...

Ngày tải lên: 07/08/2014, 18:21

... findings are in accordance with those of Cake and coworkers [9] in an ovine meniscectomy model of OA Many proteases have been shown to play major roles in the catabolism of OA cartilage For instance, ... forces present in OA In the present study, treatment with ASU reduced the level of MMP-13 synthesis in the deep zone of cartilage These findings are in accordance with a previous study in which ASU ... JC, Manning PT, Connor JR, Currie MG, Martel-Pelletier J: Selective inhibition of inducible nitric oxide synthase reduces progression of experimental osteoarthritis in vivo: possible link with the...

Ngày tải lên: 09/08/2014, 14:20

... was defined as 100%, with the nanoparticle-treated cells being calculated as percentage of the control In vivo imaging with IRDy800CW-MNPs Figure NIRF imaging of organs of a IRDy800CW-MNPsinjected ... behavior over the five days of in vivo imaging revealed that injection of the nanoparticles did not result in any observed adverse effects on activity, eating or drinking of the mouse This implies ... liver in the live-body imaging However, the organ imaging clearly revealed its importance in evaluating the biodistribution of the nanoparticles Taken together, the individual NIRF imaging of organs...

Ngày tải lên: 11/08/2014, 00:22

... decrease in iNOS expression in cytosol after inflammatory stimulation, inhibiting superoxide generation and resulting in prevention of tissue injury in patients with chronic airway diseases, including ... concentration of 2.0 μg for examining iNOS mRNA expression Poly A+ mRNA in a volume of 150 μl were added into each well of a 96-well microplate that contained 50 μl of specific probe in duplicate and incubated ... LPS-induced iNOS expression in murine macrophages and hepatocytes [36,37] The protein kinase C (PKC) pathway is also reported to be involved in LPS-induced iNOS expression, and activation of PKC isoforms,...

Ngày tải lên: 11/08/2014, 03:20

... decrease in iNOS expression in cytosol after inflammatory stimulation, inhibiting superoxide generation and resulting in prevention of tissue injury in patients with chronic airway diseases, including ... concentration of 2.0 μg for examining iNOS mRNA expression Poly A+ mRNA in a volume of 150 μl were added into each well of a 96-well microplate that contained 50 μl of specific probe in duplicate and incubated ... LPS-induced iNOS expression in murine macrophages and hepatocytes [36,37] The protein kinase C (PKC) pathway is also reported to be involved in LPS-induced iNOS expression, and activation of PKC isoforms,...

Ngày tải lên: 11/08/2014, 06:22

... expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in the timing of their expression, with TIP3;1 being abundant in embryos of mature seeds and sharply declining during seed germination, ... combining the settings indicated above in the sequential scanning facility of the microscope, as instructed by the manufacturer 3D reconstruction of z-stacks of optical sections was performed with ... set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants Plant Physiol 2001, 126:1358-1369 Ludevid D, Hofte...

Ngày tải lên: 12/08/2014, 03:21

... representing a non-invasive method that can be used to image the microscopic structure of airway wall tissue in vivo during a bronchoscopic procedure [13] The principle of this imaging method ... microfibres to the tip of the miniprobe of mm in diameter, which can be inserted into the working channel of a fibreoptic bronchoscope [14] High-quality and real-time in vivo morphological images ... stained with haematoxylin and eosin for initial analysis of the bronchial morphology One representative slide per biopsy specimen with good morphologic quality including epithelial cells, an intact...

Ngày tải lên: 12/08/2014, 13:22

... within weeks of the first infusion (P = 0.005) [27] Increased NO production of monocytes is associated with increased mitochondrial biogenesis in lupus T cells, while increased NO production of ... P, Tobin YM, Khraishi MM, Hamilton SF, Alderdice C, Richardson VJ: Elevated serum nitric oxide levels in patients with inflammatory arthritis associated with co-expression of inducible nitric ... Triptolide, an active component of the Chinese herbal remedy Tripterygium wilfordii Hook F, inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription...

Ngày tải lên: 12/08/2014, 14:22

... performed the iNOS inhibition experiment using NOS inhibitors, in non-sensitized guinea pigs with normal cough reflex sensitivity and in OVA-sensitized guinea pigs which were of increased cough ... Guinea pigs weighing 150-200 g were intraperitoneally administered with 2.0 mg of OVA and 100 mg of aluminum hydroxide [Al (OH)3] two days after an intraperitoneal administration of 30 mg/kg cyclophosphamide ... [29] These findings indicate that cNOS inhibition seems to be detrimental, and iNOS inhibition is a possible target of novel therapeutic agents in bronchial asthma Disappointingly, Singh [30] reported...

Ngày tải lên: 13/08/2014, 10:20

... Cauwels A: Nitric oxide in shock Kidney Int 2007, 72:557-565 24 Su CF, Yang FL, Chen HI: Inhibition of inducible nitric oxide synthase attenuates acute endotoxin-induced lung injury in rats Clin Exp ... protein expression determined by western blotting in lung tissue at different time points after induction of sepsis following acute lung injury Animals with sham injury served as controls including ... endothelial nitric oxide synthase; FiO2: fraction of inspired oxygen; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IL: interleukin; iNOS: inducible nitric oxide synthase; nNOS: neuronal nitric oxide...

Ngày tải lên: 13/08/2014, 21:20

... (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each other (Figure 5) Within the ... fluorescence imaging, where autofluorescence may interfere with the signal of interest [13], background luminescence is negligible This study aimed at generating a sensitive tool for noninvasive in vivo ... and nuclear noninvasive imaging Eur J Nucl Med Mol Imaging 2004 in press Ray P, De A, Min JJ, Tsien RY, Gambhir SS: Imaging tri-fusion multimodality reporter gene expression in living subjects...

Ngày tải lên: 14/08/2014, 19:22

... showing the presence of iNOS (~130kDa) in LPS rats pre-treated with flurbiprofen or nitroflurbiprofen Figure 6-3b Quantitation of liver (shown in Figure 6-3a) iNOS Figure 6-4 Effect of flurbiprofen ... NO Nitric oxide NOF (HCT-1026) Nitroflurbiprofen NOS Nitric oxide synthase nNOS (NOS I) Neuronal nitric oxide synthase NOx Nitrate/nitrite NO-NSAID Nitric oxide- releasing non-steroidal anti-inflammatory ... 2: Biology of Nitric Oxide (NO) 2.1 Biosynthesis In mammalian cells, nitric oxide (NO) arises from the enzymatic oxidation of a terminal guanidino nitrogen of the amino acid, L-arginine (Furchgott...

Ngày tải lên: 14/09/2015, 14:16