in vitro testing adhesion and delivery

... Fig In vitro transfection activity of cationic lipids in the absence of helper lipid(s) (A) and in the presence of DOPE (B) or DOPE and cholesterol (C), as measured in a murine skin cell line ... only a single amine in the polar headgroup, elicited transfection activity Addition of a second amine group and the subsequent increase in rM increased the Novel cytofectins for gene delivery ... and maintained in serum medium (Dulbeccos modied Eagles medium supplemented with 10% fetal bovine serum, 50 unitsặmL)1 penicillin and 50 lgặmL)1 streptomycin) After incubation for h at 37 C in...

Ngày tải lên: 23/03/2014, 07:20

... natural and neutralized lime and lemon juices and white household vinegar in a highly standardized in vitro testing algorithm that is currently supported by the National Institute of Allergy and Infectious ... cervical-vaginal tissues before being considered as a candidate for evaluation in clinical trials It must not cause inflammation or sloughing of the vaginal epithelium, and even minor, subclinical ... Lemon and Lime Juice and Household Vinegar Exhibit Strong pH-Dependent Cytotoxicity to Cervico-Vaginal Cell Lines In order to determine if the toxic effects observed for permanent cell lines and...

Ngày tải lên: 10/08/2014, 05:21

... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...

Ngày tải lên: 18/02/2014, 04:20

... I (NF-I) required to facilitate initiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting in origin unwinding under conditions of altered ... containing the PyV replication origin [21], used with human DNA polymerase a-primase and JCV TAg Values in panels (A) and (B), columns 2–7 were corrected for background incorporation determined in ... a-primase Standard deviations from two to four experiments are indicated as error bars The experiments in (A) and (B) were performed in parallel replication origin (pJC389) and either recombinant human...

Ngày tải lên: 17/03/2014, 03:20

... dissolved in acetic acid (100 mL), and a cold solution of amino acid (33.77 mM, i.e 2.53 g, g, 5.58 g, and 4.63 g of glycine, b-alanine, L-phenylalanine, and 4-aminobenzoic acid, respectively) in acetic ... fungi, growth in media containing sample under test were determined by linear growth (mm) measuring, and percent inhibition of growth was calculated with reference to negative control using formula ... organoantimony(III) difluorides containing Y,C,Y pincer type ligands using organotin(IV) fluorinating agents J Fluor Chem 129:167–172 Gebel T (1997) Arsenic and antimony: comparative approach on...

Ngày tải lên: 21/06/2014, 02:20

... solution containing the rutin (120 ml) was added to this organic phase maintaining the agitation for a further 10 Then, the organic phase was injected into an aqueous phase (534 ml) containing Tween ... rutin-loaded nanocapsules and nanoemulsions, as aqueous intermediate or final systems to the development of nanomedicines containing rutin Both formulations presented an increase in the rutin ... Biosystems) containing ml of the sample (0.25 mg ml-1) was put into a glass test-tube containing 200 ml of dissolution medium This system was maintained under constant moderate stirring during all the...

Ngày tải lên: 21/06/2014, 17:20

... technique are now used routinely (Webber, 1987; Webber and Painter, in preparation) However, methods for assessing pollen viability in vitro and relating the results to seed set remain rudimentary The ... of pollen grains arising from a single clone or seedling) were stored at a pollen moisture content of < 8% and at -20 °C in evacuated containers (see Webber, 1987; Webber and Painter, preparation) ... varied and ranged from 1-5 yr In vitro in lot viability assays Germination Media type The procedure for germinating Douglas fir pollen initially followed the technique described by Ho and Sziklai...

Ngày tải lên: 08/08/2014, 23:22

... cocktail containing serine/threonine phosphatase inhibitors and one containing tyrosine phosphatase inhibitors) was also used Protein kinases were digested with lysyl endopeptidase and trypsin and subsequently ... López et al.: Discovering and validating unknown phospho-sites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools Journal of Clinical Bioinformatics 2011 1:16 ... the amino acid sequence was inspected to look for indications of why these y/b-ions were missing Peptides lacking a C-terminal arginine or lysine residue, due to a C-terminal position in the...

Ngày tải lên: 10/08/2014, 09:22

... most prominent anti-PAF activity were clarithromycin, azithromycin, linezolid, amikacin and netilmicin, while in the case of rPRP were amikacin, azithromycin, tigecycline and clarithromycin These ... approximately In this case, the most potent antibiotics in the rank were netilmicin, azithromycin, amicacin and daptomycin, while again meropenem did not influence thrombin activity in WRPs Several combinations ... clarithromycin, azithromycin, linezolid, amicacin and netilmycin Other drugs studied such as meropenem and vancomycin, did not influence PAF activity in WRPs From all antibiotics tested, only vancomycin,...

Ngày tải lên: 11/08/2014, 03:20

... most prominent anti-PAF activity were clarithromycin, azithromycin, linezolid, amikacin and netilmicin, while in the case of rPRP were amikacin, azithromycin, tigecycline and clarithromycin These ... approximately In this case, the most potent antibiotics in the rank were netilmicin, azithromycin, amicacin and daptomycin, while again meropenem did not influence thrombin activity in WRPs Several combinations ... clarithromycin, azithromycin, linezolid, amicacin and netilmycin Other drugs studied such as meropenem and vancomycin, did not influence PAF activity in WRPs From all antibiotics tested, only vancomycin,...

Ngày tải lên: 11/08/2014, 06:23

... of two independently folded subunits, the N- and C-terminal domains (NTD and CTD) [4] The N-terminal domains of CAp24 are assembled into hexameric rings [5] and each hexameric ring is joined to ... in HIV strains Since mutation of Asp51 to alanine has shown to be critical for proper capsid formation and subsequent replication of the virus, we extended the above findings and examined amino ... Morphological analysis of in vitro assembled mutant CAp24 Morphological analysis of in vitro assembled mutant CAp24 proteins Mutant and wild type CAp24 proteins were induced for in vitro CAp24 tubular...

Ngày tải lên: 13/08/2014, 05:22

... in table Cell culture and transfection Huh-7 cell lines were used maintained in Dulbecco’s modified eagle medium (DMEM) supplemented with 100 μg/ml penicillin; streptomycin and 10% fetal bovine ... culture and grown as individual cell lines Once the clones had been isolated and individually grown as cell lines, the concentration of neomycin was decreased to 500 μg/ml The individual cell lines ... Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in- vitro testing system for novel anti-HCV drugs Genetic Vaccines and Therapy 2011 9:12 Submit your...

Ngày tải lên: 14/08/2014, 19:22

... performance during in vitro enzyme activity studies This work contributes to understanding the biological function of residues lining the RdRp catalytic domain in DENV NS5 Gaining insight into specific ... both finger and palm domains resulting in the closed conformation of RdRp during de novo initiation This is to restrict access to the template binding channel thereby preventing dsRNA binding to ... are coloured in grey and the GDD motif are in red box The NLS and priming loop are marked with black arrow and indicated in yellow and pink respectively The amino acid residue numbering is labeled...

Ngày tải lên: 02/10/2015, 17:14

... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript Competing ... the original loading solution determined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions XPS was used to further confirm Cu loading and to probe ... carboxylate ligands to bind Cu 2+ ; however, other metal/ligand/polymer combinations could be synthesized including those employing other polymers routinely used in targeted drug delivery, such...

Ngày tải lên: 21/06/2014, 02:20

... of cytokine interactions Previous studies showed that infusion of LPS mimics the endotoxic state and results in the production of multiple inflammatory cytokines, including IL-1, INF-γ and TNF-α ... silence the IL-10 cytokine both in vitro and in vivo and were not toxic to RAW 264.7 cells These findings suggest that JCV VLPs is an effective delivery vector for RNAi delivering with potentially ... to increase the ratio of packed shRNA template, a minimal linear DNA fragment containing HU6 promoter and shRNA template (HU6-shRNA template) was PCR amplified and packed into VLPs This linear...

Ngày tải lên: 10/08/2014, 05:21

... responsible for developing and maintaining successful TB and HIV/AIDS control programs, in addition to securing financial and organizational support for continuous and uninterrupted supply of ... TBHIV interclinic referral; poor service integration and recording; logistical shortages; and high costs of services Another South African study [16] identifying constraints to integrating TB and ... open-coding has been used in a previous South African study on HIV testing and disclosure among TB patients [27] In the current study, the information gathered in the group and individual interviews...

Ngày tải lên: 10/08/2014, 10:23

... or in Milli-Q water by centrifugation (3000 rpm/10 minutes), pelleting and resuspension The final fungal cell suspension was prepared by adjusting the inoculum to × 107 cfu/mL and then diluting ... Final DODAB, AmB and CMC concentrations were 0.1 mM, 0.05 mM and 0.1 g.L1, respectively Interactions DODAB BF/AmB and CMC took place over 20 minutes and AmB/DODAB BF/CMC and PDDA, over 30 minutes ... 0.01–2.00 mg/mL and 0.01–10.00 mg/mL, respectively Firstly, DODAB BF and drug were allowed to interact for 10 minutes Thereafter, CMC was added and allowed to interact for 20 minutes before adding PDDA,...

Ngày tải lên: 11/08/2014, 00:22

... Beta2-integrins mediate stable adhesion in collisional interactions between neutrophils and ICAM-1expressing cells J Leukoc Biol 1998, 64:622-630 Luscinskas FW, Ding H, Lichtman AH: P-selectin and ... With LPS as the single inflammatory stimuli in vitro, adhesion molecules exhibit divergent time courses of expression In this experimental setting investigating human neutrophils and endothelial ... Dickinson) with individual settings for each antibody utilizing Cell Quest Pro Software (Becton Dickinson) Unstained cells were used to discriminate autofluorescence and to adjust forward and...

Ngày tải lên: 13/08/2014, 01:20

... incorporation on the efficiency of siRNA delivery by various polycations was investigated in cell lines stably expressing reporter genes in vitro 50 Figure 17 In vitro delivery of siRNA-Luc in ... chitosans in vitro and in vivo (Paper I) Enhance the transfection efficiency of chitosan oligomer-based pDNA formulations in vitro and after lung administration in vivo by coupling a targeting moiety ... exploited to achieve targeted gene delivery in vitro and in vivo Various ligands that target cell-surface receptors (transferrin, folate, lectin, growth factors and integrin receptors) have been coupled...

Ngày tải lên: 23/08/2015, 17:29