in vitro propagation and secondary metabolite production in crocus sativus l

... and related to the following combination of explanatory variables: total length (Ltotal ), whole-body weight (W body ), Ltotal and predicted liver weight (W liver,predicted ), and Ltotal and W ... underscoring of these tiny cells, especially when large, swelling oocytes dominated in the sample This risk of visually overlooking small structures under the microscope also applied to POFs, but ... Vertical lines refer to the time of the autumnal equinox The lower horizontal line separates immature or regressing individuals (following the first spawning season;

Ngày tải lên: 04/09/2015, 12:47

... explants Shoot proliferation of P cineraria was also promoted by high levels of BA, 10 mg/I BA, mg/I NAA resulting in shoots/node K failed to induce multiple shoot production in P cineraria Shoot ... of K in combination with auxins, promote shoot proliferation probably by stimulating axillary bud growth Medium to high concentrations of auxin in combination with low to medium cytokinin concentrations ... study was P chilensis > P cineraria > P juliflora The results for all species show a similar pattern In general, IBA promotes rooting and K is less inhibitory to root production than is BA High...

Ngày tải lên: 09/08/2014, 04:20

... result in PKR-mediated inhibition of protein synthesis in a leukaemia cell line [4], this would suggest that a complex interplay of signalling pathways are involved in SMase-mediated PKR signalling ... SMase-induced increase in total collagen production is not due to increases in type I or III collagen, but further investigation is clearly warranted We propose that small increases in cellular ... 105 cells/well of a 48-well plate) were treated with sphingomyelinase (0.1 U/ml) in the presence of 20 µCi/ml of [3H]-proline and 10 µCi/ml [35S]-sulphate (GE Healthcare, Chalfont St Giles, UK)...

Ngày tải lên: 09/08/2014, 08:22

... 1.1.2 In vivo metabolic engineering and its challenges One way to overcome these challenges in using cells as bioreactor is the exploitation of cell-free synthesis In vitro cell-free synthesis plays ... in vitro Novel industrial optimization tools and systemlevel modelling approach were explored to improve the AD titer to near theoretical yield The insights gained and strategies employed will ... concept of balancing multienzymatic flux in vitro by combinatorial approach and rapidly identify the optimum enzymatic levels (2) Following the pathway balancing in vitro, we was able to achieve...

Ngày tải lên: 09/09/2015, 11:13

... the accumulation in relatively small plants as well as for understanding the basic properties of phosphorus accumulation Accumulated phosphorus in leaves [μg-P/plant] 1.7 μg/(day･plant) day 264 ... et al., 2007) And gramine (N-(1H-indol-3-ylmethyl)-N,N-dimethylamine), which is widely used as an initial compound in the synthesis of a variety of substituted indoles, can be isolated from the ... that will remain the following year are cropped before the start of the dying down period Then, after all of the above-ground part has died down, the whole plants including the roots and rhizomes...

Ngày tải lên: 05/09/2013, 09:38

... et al / VNU Journal of Science, Natural Sciences and Technology 24 (2008) 280-286 sludge for replaced material as color blending (pigment) and raw material in flower brick and cement brick production ... sludge partly replaced raw material having fine surface but more sticky and more difficulty when demolishing (table 3) Table The results of the experiment in Flower brick production Material ... material (%) In further study, heavy metal leaching into rain water with lower pH value and longer soaking time should be investigated; the use of mixing treated sludge with raw material/powder...

Ngày tải lên: 12/02/2014, 17:20

... with 100 lM neurotoxin; lane 2, a-synuclein with 200 lM neurotoxin; lane 3, a-synuclein with 100 lM neurotoxin and 50 lM dopamine; lane 4, a-synuclein with 200 lM neurotoxin and 50 lM dopamine nents ... with 200 lM neurotoxin; lane 4, with 100 lM neurotoxin and 50 lM dopamine; lane 5, with 200 lM neurotoxin and 50 lM dopamine (D) Lane M, prestained molecular mass markers; lane 1, a-synuclein with ... RNA-binding protein TLS is a major nuclear aggregate-interacting protein in huntingtin exon with expanded polyglutamine-expressing cells J Biol Chem 283, 6489–6500 Uversky VN, Li J, Bower K & Fink...

Ngày tải lên: 14/02/2014, 19:20

... consistently localized in the synovial lining layers and the interstitium that precludes the lymphoid aggregates, vascular walls, and cartilage chondrocytes in all samples (Fig 6) In the subintimal interstitium, ... synovial cells (a mixed population of synovial lining cells and interstitial fibroblasts) Although sPLA2-IIA and -V transcripts were barely detectable in unstimulated cells, they were markedly induced ... of sPLA2-IIA (Fig 2A) Thus, sPLA2-IID appears to be preferentially induced in the lymph follicular cells and capillary endothelial cells in synovial tissues with active RA Although no staining...

Ngày tải lên: 19/02/2014, 16:20

... many operating inputs, including feed, bedding and litter, fuel, repairs, and hired labor, but only those for feed and fuel were statistically different from these costs on conventional operations ... production technology (which typically confines milk cows in large barns and limits access to pasture) and because of economies of size, larger farms likely have less incentive to consider alternatives ... repairs; hired labor; other costs; and operating interest Capital ownership costs include the annualized cost of maintaining the capital investment (economic depreciation and interest) in the dairy...

Ngày tải lên: 22/02/2014, 05:20

... 6-monthly intervals once the blood glucose level and blood glucose lowering therapy are stable R18 If HbA1c levels remain above target levels, but pre-meal self-monitoring levels remain well controlled ... to develop clinical guidelines for the NHS NHS National Health Service – this guideline is written for the NHS in England and Wales NICE National Institute for Health and Clinical Excellence – ... National Institute for Health and Clinical Excellence (NICE) to undertake commissions for national clinical guidelines on an annual rolling programme Citation for this document National Collaborating...

Ngày tải lên: 08/03/2014, 14:20

... following categories: Broilers and Other Meat-Type Chickens Sold; Broiler Operations With Sales; and Total Cropland, which includes cropland harvested, cropland used only for pasture or grazing, ... Production in America product in serious oversupply, in many instances becoming a liability rather than an asset.28 Still, individual growers—not the large, well-capitalized integrators—remain primarily ... non-point pollution now dominates pollutant flows and remains the primary obstacle to cleanup Although the law designates CAFOs as point sources, it also specifically exempts “agricultural stormwater”...

Ngày tải lên: 17/03/2014, 10:20

... synoviolin-null cells and wt cells As shown in Fig 2, western blot analysis of the intracellular degradation of NCT in synoviolin-null cells and wt cells following cycloheximide treatment revealed ... synoviolinnull cells and wt cells To determine this, we detected NCT localized at the plasma membrane in synoviolinnull cells For this purpose, we labeled the cell surface proteins with biotin, and ... cells, although endogenous PS, APH-1 and PEN-2 were not changed in synoviolin-null cells Interestingly, the mobilities of immature and mature NCT on the gel in synoviolin-null cells were slightly...

Ngày tải lên: 23/03/2014, 04:20

... from these policies due to several constraints and challenges in implementation hence the need to formulate policies that will stimulate development of the local poultry in smallholder production ... Tables TABLE PRODUCTION COEFFICIENTS OF THE LOCAL CHICKEN IN UGANDA AS REPORTED BY SCHOLARS TABLE LIST OF LOCAL POULTRY STAKEHOLDERS 12 Animal Health, Breeds and Livelihoods Poultry ... Animal Health, Breeds and Livelihoods Poultry Genetic Resources and Small Poultry Production Systems in Uganda 11 Record keeping is a management tool for efficiency, decision making and planning In...

Ngày tải lên: 23/03/2014, 21:20

... findings are presented for all schools and frequently by the following school characteristics: • School instructional level: elementary school; middle school; and high school • School enrollment ... school meals program follows generally healthy eating practices: 1995 Meals program effort Reported following generally healthy eating practices Reported not following generally healthy eating practices ... percent, are the following: Collins, Janet L. , Leavy Small, M., Kann, L. , Collins Pateman, B., Gold, R., and Kolbe, L. , “School Health Education.” Journal of School Health, 65(8) (October 1995):302...

Ngày tải lên: 28/03/2014, 20:20

... protein molecules that may bind nonself plasmids during in vitro selection Two trial enrichments were carried out, in which cells expressing p50–FLAG-PRAI were diluted 103-fold and 106-fold in ... the original dilution, confirming functional epitope display and in vitro selection for a full-length (ba)8-barrel protein Selection from a FLAG-PRAI loop library The results from trial enrichments ... mgÆmL)1 herring sperm DNA, pH 7.4) A single spheroplast pellet was resuspended in mL sterile water, causing osmotic lysis The lysate was diluted to mL with p50 binding buffer, and insoluble debris...

Ngày tải lên: 30/03/2014, 20:20

... explained to them on diagnosis (N=1,127) (%) Very clearly explained 20 Fairly clearly explained 24 Neither clearly nor unclearly explained Not very clearly explained 17 Not at all clearly explained ... Striatum/cerebellum binding ratio alone52 123I-FP-CIT SPECT: Binding index in putamen contralateral to initially clinically affected side50 TRODAT-1 SPECT: Binding index in putamen contralateral to initially ... develop clinical guidelines for the NHS in England and Wales The NCC-CC was established in 2001 It is an independent body, housed within the Clinical Standards Department at the Royal College of...

Ngày tải lên: 31/03/2014, 13:20

... the kinased “short” ohgo with the “long” oligo in the following annealing mixture: 20 pL of kinased “short” oligo, 10 ILL of nmol/p ,L “long” oligo, 10 PL of 10X annealing buffer, and 60 p ,L of ... supernatant and respin briefly 24 Remove all of the remaining liquid, carefully avoiding the small RNA pellet 25 Allow the residual ethanol to evaporate with the tubes open 26 Add 5-10 pL of gel loading ... a heat lamp Place each filter into a scintillation vial, add scintillation fluid, and count Calculate the amount of labeled nucleotide incorporated into RNA (incorporated cpm) per microliter of...

Ngày tải lên: 11/04/2014, 09:47

... focal plane Nissl-stained neurons were differentiated from non-neuronal cells by the clearly defined nucleus, cytoplasm, and a prominent nucleolus After all of the cells were counted, the total numbers ... nitrocellulose filter (Millipore, Bedford, MA) using a semidry blotting apparatus (Bio-Rad Laboratories, Hercules, CA) After blocking with a solution containing 5% nonfat milk, the filters were incubated ... is induced during pathophysiological responses to inflammatory stimuli such as bacterial endotoxin, interleukin-1 (IL-1), and various growth factors [5,6] During the process of prostaglandin production, ...

Ngày tải lên: 19/06/2014, 22:20

... in epithelial cells (oral and nasal) and in peripheral lymphocytes related to FA exposure [13,14] Page of Frequency of micronucleus (MN) in buccal and/ or nasal mucosa cells is being used to investigate ... refrigerated and processed within hours of the blood collection Lymphocytes were isolated using Ficoll-Paque gradient and placed in RPMI 1640 culture medium with L- glutamine and phenol red added ... 10% inactivated fetal calf serum, 50 g/mL streptomycin + 50 U/mL penicillin and 10 g/mL phytohaemagglutinin Duplicate cultures from each subject were incubated at 37C in a humidified 5% CO2 incubator...

Ngày tải lên: 20/06/2014, 00:20

... used in the laboratory (50 lg/ml for ampicillin, 10 lg/ml for streptomycin and 50 lg/ml for kanamycin) [31] Similar results were obtained for M luteus and S aureus too (pictures not shown) We also ... ampicillin with respect to the free ampicillin was shown for all the three bacterial strains we had tested Similar data for streptomycin and kanamycin were plotted also in Fig 7b and c, respectively ... ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin, whereas Gnps conjugation might stabilize the ring and thereby allowing...

Ngày tải lên: 22/06/2014, 06:20