in vitro follicle growth and maturation

... Therefore, replacing of 20% dried and ensiled and fresh cassava forage in Napier grass diets improved weight gain Table 4.10 showed that feed intake and weight gain increased in diet ... Crantz) in diet on growth and methane production in cattle” 1.2 Research objectives To measure in vitro digestibility and methane production of feeds and different mixtures of Napier grass and dried ... grass and para grass; dried cassava forage, cotton seed meal and copra meal; mixtures of Napier grass and dried cassava forage in cattle diets using in vitro gas production technique with Sindhi

Ngày tải lên: 04/05/2016, 08:23

... X-100 and metalloproteinase activity was reactivated by incubating the gel in a buffer containing 40 mM Tris-HCl pH 7,5; 0,1 M benzamidine (SIGMA) and 10 mM CaCl2, for 72 h The gel was stained ... and loaded, using cell number for normalization Samples were run in a 10% polyacrylamide gels containing gelatin (0.05%) under non-reducing conditions SDS was removed by extensively washing in ... E-cadherin is associated to the progression from benign to malignant tumour Indeed, it was reported that in vitro re-expression of E-cadherin protein in E-cadherin negative tumour cells inhibit

Ngày tải lên: 16/01/2020, 00:35

... valued petaloid and gynomonoecious lines can only be maintained through vegetative propagation Initial in vitro axenic culture establishment, poor multiplication rates, excess callusing and vitrified ... (T3), 2.0 (T4) and 3.0 (T5) mg/l), kinetin (0.5 (T6) and 1.0 (T7) mg/l) individually and in combination (0.5 + 0.5 (T8), 1.0 + 0.5 (T9) mg/l) with NAA (0.1 mg/l) On the basis of initial experiment ... of shape, size and good keeping quality Among the floriculture crops, it is cultivated in an area of 56.04 thousand with 501.87 thousand MT production and occupied first in area and production

Ngày tải lên: 26/03/2020, 02:34

... to investigate the effect of incorporation of graded levels of dried sugar beet pulp in the concentrate mixtures on in vitro nutrient digestibility and rumen fermentation pattern using goat inoculum ... Pulpin Goat Inoculum R.S Khant, Jasmine Kaur*, R.S Grewal, J.S Lamba and P.S Chawla Department of Animal Nutrition, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India ... *Corresponding author ABSTRACT Keywords Dried sugar beet pulp, Goat inoculum, In vitro digestibility, Methane Article Info Accepted: 15 June 2018 Available Online: 10 July 2018 The main objective

Ngày tải lên: 21/05/2020, 20:43

... RNA-binding activity [12] Prokaryotes contain proteins harboring only a single CRM domain, whereas land plants contain proteins harboring multiple CRM domains Splicing of group I and group II introns ... plants, indicating the important roles of CRM domain proteins in plant growth and development [11,12,17,20,21] Despite an increased understanding of the roles of CRM domain-containing proteins in ... CFM4 is involved in the processing and maturation of 16S and 4.5S rRNAs (Figure 5B) This observation is in line with previous findings that prokaryotic proteins harboring a single CRM domain participate

Ngày tải lên: 27/05/2020, 01:43

... immunohistological staining of tumor sections showing reduced staining in sh1 and sh1b tumors staining for MMP-1 in sh1 and sh1b tumors compared with tumors of the control cell lines (Figure 3C) The incidence ... cytokines, latent growth factors, growth factor-binding proteins, and adhesion molecules [17] Understanding how MMP-1 and other members of the MMP family promote metastasis, in part by altering ... excised and immediately frozen in ornithine carbamyl transferase compound Brain sections (10 μm thick) were serially cut every 300 μm and processed for hematoxylin and eosin (H&E) staining, and viewed

Ngày tải lên: 05/11/2020, 08:01

... miR-124-3p, and that SOCS3 knock-down inhibited the expression of miR124-3p in both cell lines (Fig a, b) In addition, imatinib treatment resulted in a significant increase of miR-124-3p in CML cell lines, ... to imatinib-induced apoptosis of CML cells We examined SOCS3 expression levels in K562 and KU812 cells prior to and after imatinib treatment qPCR assays demonstrated a marked increase in mRNA ... up-regulated by imatinib a The expression of SOCS3 in K562 and KU812 cells was examined by qPCR and western blotting after they were treated with imatinib for 48 h β-Actin was served as an internal control

Ngày tải lên: 04/12/2022, 15:31

... 15-40% B in A for 20 min, 40-60% B in A for the next 5 min, then 60-100% B in A for 3 min and 100% B isocratically for 5 min; the flow rate was 1 ml min -1 . ... — Gelling agents affect growth of walnut in vitro cultured shoots. Gelrite promoted shoot elongation and bud production, whereas agar inhibited growth, induced mature ... juvenility in walnut, decreased drastically in the callus. Mineral element accumulation and decrease of hydrojuglone glucoside were evident after growth inhibition, indicating that

Ngày tải lên: 08/08/2014, 23:22

... EP1, EP2, EP3, and EP4 in the growth plate and compared this profile with their expression in cultured growth plate chondrocytes in order to give innovative insights into in situ -in vitro correla- ... However, inadequate information is avail- able on in situ expression of both COX-1 and COX-2 within the growth plate to correlate in vitro findings with the in situ situation. PGE 2 , the principal ... tion and promotes DNA and matrix synthesis in growth plate chondrocytes [7]. In addition to various findings in vitro, the physiological role of prostaglandins was clarified by its stimu- lating

Ngày tải lên: 09/08/2014, 08:22

... effective in inhibiting the function of proteins by blocking the activity domain or critical protein interaction sites There have been numerous antibody drugs undergoing pre-clinical and Du et ... DNA-PKcs is approximately 470 kD consisting of 4128 amino acids Its C-terminus contains a serine/threonine protein kinase domain (3719- 4128) [23] For screening Page of 11 the specific anti-DNA-PKcs ... protein level in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells (B) In vitro detection of Gy-induced DNA-PKcs activity using the Signa-TECT® DNA-Dependent Protein Kinase Assay System Immunoblotting

Ngày tải lên: 09/08/2014, 09:20

... study in which silence of TF expression in lung adenocarcinoma cells by TF-siRNA could inhibit tumor growth and metastasis in vitro and in vivo, and the antitumor effects may be associated with inhibition ... Nanjing Qingzilan Technology Co., Ltd (Nanjing, China). Animal treatment and care were in accordance with institutional guidelines. A549 cells(1 × 10 7 ) were suspended in 100 ul PBS and injected ... tumor growth, vascularization, and metastasis in lung adenocarcinoma. Conclusions In summary, our findings clearly demonstrate that TF plays a crucial role in lung adenocarcinoma tumor growth and

Ngày tải lên: 10/08/2014, 10:21

... Journal of Inflammation Open Access Research Thrombin increases inflammatory cytokine and angiogenic growth factor secretion in human adipose cells in vitro Jennifer L Strande* 1 and Shane A ... inhibitor lepirudin given in conjunction with thrombin prevented the thrombin- mediated increase in cytokine and growth factor secretion. Conclusion: Here we show that thrombin PAR1 and PAR4 receptors ... receptors are present and that thrombin stimulates inflammatory cytokine generation and growth factor release in human adipose tissue and cells in vitro. These data suggest that thrombin may represent

Ngày tải lên: 11/08/2014, 08:22

... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...

Ngày tải lên: 18/02/2014, 04:20

... Fig In vitro transfection activity of cationic lipids in the absence of helper lipid(s) (A) and in the presence of DOPE (B) or DOPE and cholesterol (C), as measured in a murine skin cell line ... and maintained in serum medium (Dulbeccos modied Eagles medium supplemented with 10% fetal bovine serum, 50 unitsặmL)1 penicillin and 50 lgặmL)1 streptomycin) After incubation for h at 37 C in ... series by only a single amine in the polar headgroup, elicited transfection activity Addition of a second amine group and the subsequent increase in rM increased the Novel cytofectins for gene delivery...

Ngày tải lên: 23/03/2014, 07:20

... technique are now used routinely (Webber, 1987; Webber and Painter, in preparation) However, methods for assessing pollen viability in vitro and relating the results to seed set remain rudimentary The ... of pollen grains arising from a single clone or seedling) were stored at a pollen moisture content of < 8% and at -20 °C in evacuated containers (see Webber, 1987; Webber and Painter, preparation) ... varied and ranged from 1-5 yr In vitro in lot viability assays Germination Media type The procedure for germinating Douglas fir pollen initially followed the technique described by Ho and Sziklai...

Ngày tải lên: 08/08/2014, 23:22

... tumorigenesis and metastasis in mice inoculated with Trip10-overexpressing IMR-32 cells (Figure 4A) On the other hand, huntingtin increases cell death by promoting apoptosis Thus, high levels of huntingtin ... distributed in the cytosol of CP70 control cells (Figure immunostaining) Overexpression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and form perinuclear foci In contrast, ... Cdc42 is involved in migration; therefore, strong Cdc42 expression in IMR-32 cells may cause them to become more invasive, possibly explaining the enhanced in vitro colony formation and in vivo...

Ngày tải lên: 10/08/2014, 05:21

... cocktail containing serine/threonine phosphatase inhibitors and one containing tyrosine phosphatase inhibitors) was also used Protein kinases were digested with lysyl endopeptidase and trypsin and subsequently ... López et al.: Discovering and validating unknown phospho-sites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools Journal of Clinical Bioinformatics 2011 1:16 ... the amino acid sequence was inspected to look for indications of why these y/b-ions were missing Peptides lacking a C-terminal arginine or lysine residue, due to a C-terminal position in the...

Ngày tải lên: 10/08/2014, 09:22

... most prominent anti-PAF activity were clarithromycin, azithromycin, linezolid, amikacin and netilmicin, while in the case of rPRP were amikacin, azithromycin, tigecycline and clarithromycin These ... approximately In this case, the most potent antibiotics in the rank were netilmicin, azithromycin, amicacin and daptomycin, while again meropenem did not influence thrombin activity in WRPs Several combinations ... clarithromycin, azithromycin, linezolid, amicacin and netilmycin Other drugs studied such as meropenem and vancomycin, did not influence PAF activity in WRPs From all antibiotics tested, only vancomycin,...

Ngày tải lên: 11/08/2014, 03:20

... most prominent anti-PAF activity were clarithromycin, azithromycin, linezolid, amikacin and netilmicin, while in the case of rPRP were amikacin, azithromycin, tigecycline and clarithromycin These ... approximately In this case, the most potent antibiotics in the rank were netilmicin, azithromycin, amicacin and daptomycin, while again meropenem did not influence thrombin activity in WRPs Several combinations ... clarithromycin, azithromycin, linezolid, amicacin and netilmycin Other drugs studied such as meropenem and vancomycin, did not influence PAF activity in WRPs From all antibiotics tested, only vancomycin,...

Ngày tải lên: 11/08/2014, 06:23

... of two independently folded subunits, the N- and C-terminal domains (NTD and CTD) [4] The N-terminal domains of CAp24 are assembled into hexameric rings [5] and each hexameric ring is joined to ... in HIV strains Since mutation of Asp51 to alanine has shown to be critical for proper capsid formation and subsequent replication of the virus, we extended the above findings and examined amino ... Morphological analysis of in vitro assembled mutant CAp24 Morphological analysis of in vitro assembled mutant CAp24 proteins Mutant and wild type CAp24 proteins were induced for in vitro CAp24 tubular...

Ngày tải lên: 13/08/2014, 05:22