in vitro culture of embryonic kidney rudiments and isolated ureteric buds

... on the transdermal delivery of various molecules, both in vitro and in vivo These methods include in vitro and in vivo delivery of insulin, mannitol, glucose and heparin (17, 80) Phonophoresis ... events include extrusion of lamellar bodies, loss of nucleus and increasing amount of keratin in the stratum corneum (12) The origins of the cells of the epidermis lie in the basal lamina between ... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ...

Ngày tải lên: 13/04/2014, 09:25

... abscission Rooting of P cineraria was obtained only with combinations of K and IBA (0.05 mg/I K, 1-15 mg/I IBA, and mg/I K, 15 mg/I IBA), the greatest number of roots (2.8/ explant) being initiated ... promotes rooting and K is less inhibitory to root production than is BA High concen- trations of BA, but not of K in combination with auxins, promote shoot proliferation probably by stimulating axillary ... Shoot proliferation of P cineraria was also promoted by high levels of BA, 10 mg/I BA, mg/I NAA resulting in shoots/node K failed to induce multiple shoot production in P cineraria Shoot growth...

Ngày tải lên: 09/08/2014, 04:20

... Epstein and Lavee (1986) reported a transformation of IBA into IAA during in vitro culture of Vitis vinifera and Olea europea The chemical pathway could be a 0-oxidation of the butyric side chain, ... horticoles et culture in vitro Rev For Fr 38, 60-68 Epstein E & Lav6e S (1986) Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of gravepine (Vitis vinifera) and olive (Olea ... mechanism of this process remains unknown From the experiments run in the presence and in the absence of BA in the culture medium (Fig B), we postulate that IBA might control the penetration of BA into...

Ngày tải lên: 09/08/2014, 04:20

... written information and written consent All volunteers were healthy and free of medication Blood was taken from a basilic vein using an 18gauge IV catheter and collected in both citrated and heparinzed ... within 30 minutes [11] Estimating average plasma osmolarity of 300 mOsmol*l-1 and an osmolarity of 2400 mOsmol*l-1 for HyperHaes in vitro dilution by 5% would suggest a resulting osmolarity of ... These in vitro high osmolarity conditions could compromise the translation of the results into clinical settings Nevertheless, it Hanke et al Scandinavian Journal of Trauma, Resuscitation and Emergency...

Ngày tải lên: 13/08/2014, 23:20

... shoot, callus and adventitious root production of Vietnamese ginseng, and TLC and HPLC fingerprint methods were utilized for investigating the saponin content biomass of calli MATERIALS AND METHODS ... died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off ... died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off...

Ngày tải lên: 20/04/2015, 21:33

... 12 Lee, E S and Fukui Y Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by in vitro produced bovine morulae and blastocysts ... Production of viable bovine blastocysts in defined in vitro conditions Biol Reprod 1995, 52, 1410-1417 10 Keskintepe, L and Brackett, B G in vitro developmental competence of in vitro- matured bovine ... effects of glucose and/ or phosphate were examined in Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System 75 Ta ble Compostion of...

Ngày tải lên: 07/08/2014, 17:22

... the luc+ gene in lines 156, 49 and 111 In addition, T-DNA tandem repeats were identified in lines 156 and 49, and vector backbone sequences were integrated in lines 17, 156, 49 and 179 (data ... were recorded with an integration time of 20 Time lapses were taken at an interval of 30 for screenings on ZZ and RD2 medium, and 20 for screenings of in vitro plantlets maintained on REG medium ... active in proliferating meristem cultures We also investigated transcription of all 3' tagged sequences of line 17 by RT-PCR in cell colonies (stage I) as well as leaf and root tissues of in vitro...

Ngày tải lên: 12/08/2014, 03:20

... a combination of such factors (Chapter 4) In such Analysis, sequencing and in vitro expression of PCR products 89 cases it is often possible to increase the sensitivity of the analysis in order ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be expressed and...

Ngày tải lên: 25/10/2013, 22:20

... (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments Possible slippage mechanisms are discussed Results and Discussion Effect of DDI-1A and ... change in Tm (DTm) for the ATT bulge increased by 3.4 and 1.7 °C in the presence of DDI-1A and DDI-1B, respectively The increase in DTm for DDI-1A was higher than that for DDI-1B, implying that intercalation ... binding pocket, with its amino sugar anchored Expansion of DNA repeat sequences in the major groove of the DNA pointing toward the 3¢-bulge-flanking base pair We were interested in small molecules that...

Ngày tải lên: 07/03/2014, 06:20

... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... filters and wash any remaining larvae into individual labelled petri dishes and stain with a few drops of helminthological iodine 10 Add 50 μL of helminthological iodine to each well on the culture ... to the methods involved in collecting, preparing and screening plants for bioactive properties for use in manipulating key ruminal fermentation pathways and against gastrointestinal pathogens...

Ngày tải lên: 15/03/2014, 04:20

... ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the ... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...

Ngày tải lên: 16/03/2014, 11:20

... available and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in ... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... filter binding assay in TK8 buffer with nM I-AniI and varying concentrations of end-labeled pCOBLE or uniformly labeled AnCOB pre-RNA RNA binding reactions contained mM MgCl2 and DNA binding reactions...

Ngày tải lên: 17/03/2014, 10:20

... quality and the type of mineral formation obtained in the mineralization medium during the GPIbALP-induced hydrolysis of different phosphate substrates Ó FEBS 2003 Bone GPI-bALP, roles in mineralization ... interpretation of IR spectra of mineral deposits Distinct mineralization steps were observed during the GPI-bALPinduced hydrolysis of b-GP At incubation times in the range 1.5–3 h, two bands located ... that induce mineralization b-GP has been routinely supplemented in bone cells, chondrocytes and cultured cell types to initiate mineralization and to better monitor different aspects of mineral...

Ngày tải lên: 23/03/2014, 17:21

... cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52] Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and enhances ... by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine, 100 U/ml penicillin, and 0.1 mg/ml streptomycin The ... cells in vitro assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in TIL Media...

Ngày tải lên: 18/06/2014, 15:20

... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing LacZ gene ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood...

Ngày tải lên: 18/06/2014, 18:20

... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining of ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection, ... percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E Blocking of T-cell migration in reconstruct T-cell migration in the...

Ngày tải lên: 18/06/2014, 19:20

... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing LacZ gene ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood...

Ngày tải lên: 20/06/2014, 01:20

... cell lines and/ or due to the method used to induce Se deficiency in our in vitro model In studies employing Jurkat E6 cells, the Se-deficient medium comprised of insulin, transferrin and bovine ... medium containing 1% FBS, insulin and transferrin was adequate for growth of Vero and SK-N-SH cells for 10–12 days Se-deficient cells were maintained for 10 days and passaged every days using serum-free ... achieved in both the Vero and SK-N-SH cell lines In vitro response of Vero and SK-N-SH cells to Se deficiency Figure In vitro response of Vero and SK-N-SH cells to Se deficiency Vero and SK-N-SH...

Ngày tải lên: 20/06/2014, 01:20

... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining of ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection, ... percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E Blocking of T-cell migration in reconstruct T-cell migration in the...

Ngày tải lên: 20/06/2014, 03:20

... was incubated at 42°C for 90 min, and terminated by heat inactivation at 70°C for 15 Measurement of GAG levels The level of GAG appearing in the medium of explants, cell cultures, and papain-digested ... metalloproteinases mediating matrix degradation in interleukin 1-stimulated human articular cartilage Matrix 1993, 13:95-102 Vincenti MP, Clark IM, Brinckerhoff CE: Using inhibitors of metalloproteinases ... non-silencing siRNA; and G.4 = MMP-3 siRNA 70% due to RNA interference [26] And NF-kBp65-specific siRNA can inhibit the expression of COX-2, NOS-2 and MMP-9 in IL-1β-induced and TNFα-induced chondrocytes...

Ngày tải lên: 20/06/2014, 04:20