in vitro antitumor activity

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

Ngày tải lên : 19/02/2014, 06:20

... the hinge loop after ligation Also in this case no clashes were detected at Electrostatic interactions and antitumor RNases the dimer interface of the models or in the surroundings of the hinge ... homologous to the RNases investigated in this study, have been isolated from human eosinophils, namely eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) They are RNases ... cationic proteins, with high isoelectric point values Thus we investigated the effects Table Natural and engineered RNases investigated in this study IC50 is the RNase concentration producing half-maximal...

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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx

Ngày tải lên : 23/03/2014, 07:20

... Fig In vitro transfection activity of cationic lipids in the absence of helper lipid(s) (A) and in the presence of DOPE (B) or DOPE and cholesterol (C), as measured in a murine skin cell line ... series by only a single amine in the polar headgroup, elicited transfection activity Addition of a second amine group and the subsequent increase in rM increased the Novel cytofectins for gene delivery ... 7D), suggesting a minimal inuence of electrostatic interactions on cell internalization of 1,3lbpDNA complexes in vitro 40 30 20 10 1,3lb1 1,3lb2 1,3lb3 1,3lb4 1,3lb5 Fig Lipid mixing, as assessed...

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Báo cáo hóa học: " Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies" pptx

Ngày tải lên : 21/06/2014, 17:20

... solution containing the rutin (120 ml) was added to this organic phase maintaining the agitation for a further 10 Then, the organic phase was injected into an aqueous phase (534 ml) containing Tween ... Biosystems) containing ml of the sample (0.25 mg ml-1) was put into a glass test-tube containing 200 ml of dissolution medium This system was maintained under constant moderate stirring during all the ... rutin-loaded nanocapsules and nanoemulsions, as aqueous intermediate or ﬁnal systems to the development of nanomedicines containing rutin Both formulations presented an increase in the rutin...

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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt

Ngày tải lên : 07/08/2014, 14:20

... 10-methoxy-5H-dibenz[b,f]azepine (Compound d) 10-methoxy-5H-dibenz[b,f]azepine was prepared by brominating N-acetyl-5H-dibenz[b,f]azepine (2.35 g, 10 mM) using bromine (3.2 g, 20 mM) in dichloromethane (25 ml) to obtain ... theoretical information for the medicinal development and supplies some in vitro methods for quickly optimising drugs; it is attracting increased scientific attention from bioorganic and medicinal chemists ... outlined in schemes 1–3 In scheme 3, to obtain compound (e), the reaction was carried out by using a weak base (triethyl amine) with COCl2 at room temperature (RT) instead of triphosgene in the...

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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx

Ngày tải lên : 10/08/2014, 05:21

... particles in vitro [25], caused lesions in the vaginal epithelium in vivo and increased the probability of being infected with HIV-1 [26] To assess whether biological activity of C14 causes inflammation ... showed vaginal irritation indexes within ranges indicating that this compound may be likely suitable for vaginal use in humans [28,29] Conclusion In conclusion, the high anti-HIV-1 activity, ... cell-specific HIV-1-binding protein that enhances trans-infection of T cells Cell 2000, 100:587-97 Turville SG, Vermeire K, Balzarini J, Schols D: Sugar-binding proteins potently inhibit dendritic...

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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf

Ngày tải lên : 11/08/2014, 21:21

... compounds were analysed for in- vitro antiviral activity against HCV NS3SP, among which Epicatechin and Epigallocatechin and their dimers has in- vitro antiviral activity against HCV NS3-SP [39] Similarly ... glycyrrhizin sulphate, inhibit HIV replication, interfere with virus-to-cell binding and cell-to-cell infection, and induce IFN activity [25] Silybum marianum (milk thistle) has been shown to have clinical ... plasmids containing HCV NS3 protease in the presence and absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s...

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Báo cáo sinh học: "Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer" docx

Ngày tải lên : 14/08/2014, 19:22

... Discussion In this report, transfection of CIK cells with IL-2 demonstrated a prominent augmentation of antitumor immunity in vitro against pancreatic carcinoma cell lines via secreting significant ... to induce an immune attack against the tumor by inserting a cytokine gene into the immunological effector cells The aim of this study was to evaluate the antitumor immune responses of a cytokine ... effective in preventing tumor growth than systemic administration in patients with metastatic renal cell carcinoma [22] There are several reports introducing IL-2 producing genes into pancreatic...

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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf

Ngày tải lên : 30/03/2014, 08:20

... N-terminal domains, thus rendering intriguing the possibility that PC1 ⁄ CT-peptide might by itself possess intrinsic inhibiting properties Production of recombinant CT-peptide We expressed in bacteria ... tethering, thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the case ... puriﬁcation of recombinant mPC1 ⁄ and hfurin Recombinant murine PC1 ⁄ was produced using the baculovirus expression system in Sf9 insect cells [7] or through intracoelemic injection in insect larvae...

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Báo cáo hóa học: " First-line chemoimmunotherapy in metastatic breast carcinoma: combination of paclitaxel and IMP321 (LAG-3Ig) enhances immune responses and antitumor activity" pptx

Ngày tải lên : 18/06/2014, 16:20

... compare the immunomonitoring and clinical values obtained at the different time points Only patients with all available time points were included in the immunomonitoring analysis Spearman rank ... IL, Lokshin A, Rosenbloom A, Shurin MR, Shurin GV: Low-dose paclitaxel prior to intratumoral dendritic cell vaccine modulates intratumoral cytokine network and lung cancer growth Clin Cancer ... (day 170) injection for detection of serum anti-IMP321 antibodies Blood samples were collected in lithium heparincontaining tubes at the same time points and also two weeks after the sixth injection...

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Báo cáo sinh học: " Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" pptx

Ngày tải lên : 19/06/2014, 08:20

... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... cleavage profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a...

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báo cáo hóa học:" Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" docx

Ngày tải lên : 20/06/2014, 04:20

... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... cleavage profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a...

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Báo cáo hóa học: " Silver and Gold Nanoparticles Alter Cathepsin Activity In vitro" potx

Ngày tải lên : 21/06/2014, 08:20

... decrease in cathepsin B activity is observed in all treatment groups except for the 25-nm PS-Ag, which interestingly had no effect on cathepsin B activity (Figure table) Cathepsin L Activity in Ag-NP-treated ... utilizes the fluorophore, cresyl violet (CV), linked to two peptides moieties Page of (arginine-arginine for cathepsin B and phenylalaninearginine for cathepsin L), which are cleaved when the enzyme ... cathepsin L activity was observed Conversely, a decline in cathepsin L activity was observed when the Au-NPs were incubated with the purified enzyme This may indicate that the Au-NPs are interacting...

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Báo cáo y học: "Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro" pptx

Ngày tải lên : 09/08/2014, 14:22

... [29] TNFα induced a dose-dependent increase in GAG release supporting data from other investigations on pro-inflammatory cytokines in which IL-1 promoted GAG release in lapine and porcine meniscal ... meniscus following acute exposure to interleukin-1 in vitro J Orthop Res 2008, 26:504-512 McNulty AL, Weinberg JB, Guilak F: Inhibition of matrix metalloproteinases enhances in vitro repair of ... using 0.1% (w/v) gelatin and 0.2% (w/v) casein as substrate in 4.5 to 15% gradient SDS-PAGE, washed in 2.5% (v/v) Triton X-100, rinsed in distilled water and incubated for 16 hours at 37°C in...

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Báo cáo y học: "Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity" potx

Ngày tải lên : 10/08/2014, 05:21

... assay of in vitro migration and invasion activity The results showed that knocking down HSPA4 or HSPA14 in H1299NBS1 cells caused a significant decrease in the in vitro migration and invasion activity, ... decreased in vitro migration and invasion activity To test the role of HSPA4 and HSPA14 in in vitro migration and invasion activity, siRNA-mediated repression of HSPA4 or HSPA14 was carried out in H1299 ... the protein sequence of HSF4b Since HSF4b was shown to be phosphorylated by ERK to increase its DNA binding activity [27], it will be interesting to test whether the ERK activity could be induced...

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Báo cáo y học: " Antiretroviral activity of the aminothiol WR1065 against Human Immunodeficiency virus (HIV-1) in vitro and Simian Immunodeficiency virus (SIV) ex vivo" pptx

Ngày tải lên : 10/08/2014, 05:21

... (ATCC, Manassas, VA) containing 10% fetal bovine serum (Hyclone, Logan, UT), 1% penicillin/streptomycin/ glutamine (Invitrogen, Gaithersburg, MD), 10 U/ml interleukin (IL2, BD Biosciences, San ... doses in vivo, may have clinical utility In addition, in combination studies using both AZT and WR1065 in human TCBs, we found that addition of WR1065 to a non-saturating dose of AZT resulted in ... not saturate virus inhibition, allowing for further inhibition when WR1065 was added (see Table 3, right column) Due in part to interindividual differences in growth, HIV-1 infection capacity,...

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báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma" pot

Ngày tải lên : 10/08/2014, 10:21

... important single cytokine in inducing antitumor immunity In Page of experimental tumor models, recombinant IL-12 has demonstrated marked antitumor effects through mechanisms of both innate and ... Lungs, liver and brains of dead mice were removed and fixed in formalin, embedded in paraffin, Page of and sectioned at μm Hematoxylin & eosin (H&E) stained samples were examined under a light ... effective cells in interleukine 12 induced T cell-dependent tumor rejection Cancer Res 2002, 62:5069-5075 Colombo MP, Trinchieri G: Interleukin-12 in anti-tumor immunity and immunotherapy Cytokine & Growth...

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báo cáo khoa học: "Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro" potx

Ngày tải lên : 11/08/2014, 00:22

... allowed to interact for 10 minutes Thereafter, CMC was added and allowed to interact for 20 minutes before adding PDDA, which was also allowed to interact for 20 minutes, before determining zeta-average ... or in Milli-Q water by centrifugation (3000 rpm/10 minutes), pelleting and resuspension The final fungal cell suspension was prepared by adjusting the inoculum to × 107 cfu/mL and then diluting ... substantially reduce fungus viability For final coverage with polylysines (PL) of increasing molecular weight at mg/mL PL, there was an increase in the final zeta-potential modulus and a larger...

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Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment" pptx

Ngày tải lên : 11/08/2014, 08:21

... (FCS, PAA, Pasching, Austria), 25 mM MgCl2, mM L-glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin) Cells were incubated in serum-free MEM containing μg/ml trypsine, mM L-glutamine, 100 U/ml ... or Hep-2 cells infected with HSV-1 (data not shown) However, in HeLa cells infected with inactivated HRV an increase in IFN production was obtained in both assays A 56%-increase in IFN release ... the interpretation of the data and were highly involved in drafting and revising the manuscript BS has given final approval of the version to be published Competing interests The complex combination...

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Báo cáo y học: "Bioavailable constituents/metabolites of pomegranate (Punica granatum L) preferentially inhibit COX2 activity ex vivo and IL-1beta-induced PGE2 production in human chondrocytes in vitro" pps

Ngày tải lên : 11/08/2014, 08:22

... lipoxygenase activity and production of prostaglandins E2 (PGE2) in vitro, suggesting that the primary effect of delphinidins (also present in pomegranate fruit) may be against inflammatory responses ... exerted a powerful influence in inhibiting the expression of inflammatory cytokines IL-1β and IL-6 in adjunctive periodontal therapy [33] Other in vitro studies have shown that the bioactivity of total ... in the culture medium indicating enhanced eicosanoid generating enzyme activity in chondrocytes Interestingly, chondrocytes stimulated with IL-1β in the presence of control plasma showed no inhibition...

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Báo cáo y học: " In vitro evaluation of marine-microorganism extracts for anti-viral activity" pot

Ngày tải lên : 12/08/2014, 04:20

... high inhibition of CCV replication in CCO up to 48 hr post-infection, which decreased slightly by 84 hr post-infection All remaining extracts tested against CCV in CCO cells were determined to ... of 12.5 μg/ml in the latter experiments involving EPC cells Viral Attachment/Entry Inhibition Since little is known about the antiviral nature of these marine extracts at the beginning of these ... as having no meaningful inhibition (< 20%), slight inhibition (≥ 20%), moderate inhibition (≥ 50%), or high inhibition (≥ 80%) Viral Attachment/Entry Inhibition Assay Viral Replication Inhibition...

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