in the course of malignant disease

... an increase in p21waf1 expression, loss of CDK function, and the generation of cells with senescence characteristics In the first part of the investigation, we determined the effect of continuous ... particular phase of the cell cycle, and was associated with an increase in the sub-G1 (apoptotic) portion of the cell cycle We next investigated the effect of combining CDO with the chemotherapeutic ... protein, as seen in HCT116 cells, resulted in the absence of enhanced cell growth inhibition The central role of p21waf1 in mediating senescence is currently being investigated in isogenic cell lines...

Ngày tải lên: 07/03/2014, 15:20

... (OHEA) within the Office of Research and Development, with major contract funding provided by the Indoor Air Division within the Office of Air and Radiation's Office of Atmospheric and Indoor Air ... Average cotinine/creatinine levels for subgroups of nonsmoking women defined by sampling categories of exposure or by self-reporting exposure to ETS from different sources during the days preceding ... editing, word processing, and proofreading the final report Robert Flaak, Assistant Staff Director of the SAB, whose efforts and professionalism in organizing and coordinating the two SAB reviews...

Ngày tải lên: 15/03/2014, 01:20

... 28 from inclusion) over baseline As to the assessment of the quality of analgesic care, the adequacy of pain management will be assessed firstly using the IPM, an index assembled by grouping with ... [19,23,24] The relevant endpoint is the reduction in Adequacy of analgesic-drug therapy: Patient Management index (PMI), categorized in a binary variable according to negative (indicating inadequate ... drug (750) during the longitudinal evaluation period Overall, considering the inception and the longitudinal evaluation, the entire duration of the study is estimated around months At the moment,...

Ngày tải lên: 20/06/2014, 15:20

... is therefore possible that CKBM could induce cytokine release including TNF-α in these animals and inhibit tumor growth However, further study is needed to determine how they are induced and the ... action of CKBM is perhaps through the induction of apoptosis and inhibition of proliferation of gastric cancer cells However, further clinical trials in humans are needed to examine the pharmacokinetics ... Officer of CK Life Sciences Limited Dr Pang was the Head of Department of Physiology at University of Hong Kong prior joining the company He had been the founding Editor and Editor -in- Chief of...

Ngày tải lên: 08/08/2014, 18:20

... cause In breast cancer patients it has been reported that the severity of dermatomyositis follows the clinical course of the malignancy [4] The severity of this patient's symptoms and the level of ... no visceral disease Biopsy of the axillary lymph node confirmed the diagnosis of an oestrogen receptor (ER) positive invasive carcinoma of mammary type Mammography and ultrasound of the breasts ... http://www.wjso.com/content/6/1/132 Competing interests The authors declare that they have no competing interests Authors' contributions KLC and AH treated the patient and conceived the idea JW performed the literature...

Ngày tải lên: 09/08/2014, 07:22

... In addition, we examined the morphology of the cell nuclei to quantify the rate of apoptosis by the use of DAPI staining The results observed in Western blot analyses were supported by the findings ... analyzed the influence of OPN silencing confirmed the impact of OPN expression on the efficacy of irradiation Solberg and co-workers [43] found that irradiation of xenograft tumors in mice induces the ... Germany) linear accelerator at a dose rate of Gy/min Referring to the fractionated daily dose in therapy treatment and DMF 10 -value of the MDA-MB-231 cell line, we have chosen a radiation dose of Gy...

Ngày tải lên: 09/08/2014, 09:20

... rpm for min, 500 μL of 1×binding buffer, μL of FITC-labeled Annexin V and 10 μL of PI were added to the cell suspension and gently mixed After incubation at 25°C for 10 in the dark, the cells ... Analysis of the data and plotting of the figures were performed with the aid of software (Origin Version 7.5 and SPSS Version 13.0) Results Expression of b1- and b2-adrenergic receptors in SGC7901 ... expressed the proteins of the b1- and b2-ARs lower than the irradiation-only group In addition, the pretreatment of isoproterenol had the opposite effect and reduced the downregulation of gene...

Ngày tải lên: 09/08/2014, 09:20

... low levels of Bcl-2 in the PC3 cells may explain why the radiation-induced down-modulation of Bcl-2 was of minor importance for the response of PC3-cells to radiotherapy and the combined treatment ... based on their ability to induce apoptosis in tumor cells and their ability to increase cytotoxic efficacy of chemotherapy and radiotherapy in preclinical investigations [10-12] Induction of apoptosis ... time point, but does not indicate whether the therapy effects observed are due to inhibition of proliferation, cell death induction, or both Therefore, in a next step, we examined whether the anti-neoplastic...

Ngày tải lên: 09/08/2014, 09:20

... to be involved in tissue repair in vitro [12] and to play an important role in the tumour microenvironment and tumour cell invasion [13] In the light of these findings, it could be hypothesized ... activates other MSPs and MMPs [8,9] TATI is a trypsin inhibitor that balances concentrations of trypsin and also functions as a weak inhibitor of other serine proteinases [10,11] In addition, ... [15] In line with previous findings [1], TATI was expressed in the cytoplasm, and the percentage of positive tumour cells was estimated separately in each core Intensity was annotated using a...

Ngày tải lên: 09/08/2014, 09:21

... density values of the rectal wall often very similar to the density of the filling a precise delineation of the inner contour of the rectal wall was difficult for some patients resulting in asymmetric ... delineation of the inner and outer contour of the rectal wall 2) rectal wall based on manual delineation of the outer contour of the rectal wall and automatic calculation the inner contour using ... dose to the rectum in the mid-dose region by 6.3% ± 7.2% In the IMRT treatment plans the influence of the rectal volume on the relationship between dose parameters was different The order of the...

Ngày tải lên: 09/08/2014, 10:21

... carcinogenesis by promoting the expression of proto-oncogenes or by inhibiting the expression of tumor suppressor genes For instance, the down-regulation of let-7, which represses expression of the ... but depleted in the 3' UTRs of down-regulated genes[20] Motivated by these findings, several studies have demonstrated the effectiveness of investigating miRNA regulation by examining their target ... [3,47] In addition, the conservation of binding site, the context of the miRNA-binding site, the proximal AU composition, and proximity to sites for co-clustered miRNAs can enhance the targeting...

Ngày tải lên: 09/08/2014, 20:20

... regulating the activities of phosphotyrosine phosphatases [23] In the current study, we examined XAF1 mRNA and protein expression in four cell lines, and determined regulatory effects of somatostatin ... and MEK inhibitor-induced apoptosis in vitro [12,26-29] Moreover, some researchers have recently indicated the effect of XAF1 combination with these factors on inhibition of tumour growth in vivo ... invasiveness in the development of prostate cancer The antiproliferative effect of somatostatin may result from increased apoptosis In breast cancer cells MCF-7, the cytotoxic effect of somatostatin is...

Ngày tải lên: 10/08/2014, 10:20

... binder-1), induces PI3K activation, triggers the PI3K/Akt pathway, and is involved in the progression of breast cancer[28] It is also confirmed that the binding of CD44 to HA induces c-Src kinase ... 95% The above findings indicate that CD44 is involved in several signal transduction pathways related to tumor cell metastasis, and that inhibiting the expression of CD44 or blocking its binding ... Selection of human ovarian carcinoma cells with high dissemination potential by repeated passage of the cells in vivo into nude mice, and involvement of Le(x)determinant in the dissemination potential...

Ngày tải lên: 10/08/2014, 10:21

... described as “naphthofuranone” by the National Cancer Institute (NCI) in their in vivo“ anti-tumor screening data, testing the drug against P388 Leukemia in CD2F1 mice, however, no final conclusive ... gynaecological malignancy in many countries, in spite of chemotherapy with Platinum derivates and/or Taxol after surgery Of the malignant epithelial tumors (>90% of all ovarian cancers), the serous papillary ... Changes in abdomen size for control and treated mice In spite of the introduction of new drugs into the management of ovarian cancer there is still need for more novel treatments Conclusion The compound...

Ngày tải lên: 10/08/2014, 10:21

... exert its inhibitory effect on proteolytic activity of plasmin by binding to tumor cells through its C-terminal domain II, the major anti-fibrinolytic group The impact of ulinastatin on uPA is ... reflects the number of cells penetrated the Matrigel and membrane of the Transwell As shown in Figure 1, the invasion rates of cells treated with ulinastatin, docetaxel and ulinastatin plus docetaxel ... to its inhibitory effect on the translocation of protein kinase C from the cytoplasm to the membrane and consequent down-regulation of MEK/ERK/c-Jun pathway, thereby causing the decline in uPA...

Ngày tải lên: 10/08/2014, 10:21

... staining scored as 0, with mild brown staining scored as 1, with moderate brown staining scored as 2, with intense brown staining scored as The final positive scores = positive rate score × staining ... that MACC1 might implicate in growth and metastasis of ovarian carcinoma In ovarian carcinoma cells, the antitumor effects of MACC1 RNAi might involve in the inhibition of HGF/ Met and MEK/ERK pathways ... carcinoma patients are suffered with advanced disease, and the majority will relapse and die of their disease [11,12] Despite major efforts in diagnosis and improvements in the treatment of epithelial...

Ngày tải lên: 10/08/2014, 10:21

... cotton swab The cells that migrated to the bottom surface of the insert were fixed in 100% methanol for min, stained in Giemsa for min, rinsed in PBS and then subjected to microscopic inspection ... predominant in coding regions of some active genes [22-25] The intragenic permissive chromatin regions are flanked by the repressive mark, H3K9me3, and the maintenance of the intragenic chromatin ... loading control, GAPDH [10] the cells were incubated for 72 h (invasion) or 36 h (migration) at 37°C in a 5% CO2 incubator, the cells on the top surface of the insert were removed by wiping with...

Ngày tải lên: 10/08/2014, 10:21

... containing μl of DNA template, μl of × Master Mix, μl ddH2O and μl of each primer The relative changes in the extent of promoter methylation were determined by measuring the amount of promoter in immunoprecipitated ... expressions were also increased These results are in accordance with the findings of other reports [15-19], which could be important in the development of new and effective strategy in cervical treatment ... re-expression of CXCL12 in MCF-7 breast cancer as well as AsPC1 in pancreatic carcinoma cell lines, and suggested that they acted synergistically in inhibiting CpG island hypermethylation of tumor...

Ngày tải lên: 10/08/2014, 10:21

... lead to the release of cytokines including interleukin (IL)-12, IL-6, tumor necrosis factor (TNF)-α, and IL-10 Some of these cytokines may play important role in the cancer therapy On the other ... iC3b leads to an increase in the lysis of the target The beta chain of the CR3 molecule (CD18) rather than the alpha chain (CD11b) is responsible to the β-glucan binding [51,52] studies, structurally ... effectively to our cancer patients in the future Competing interests The authors declare that there is no conflict of interests, including conflicts of financial nature involving any pharmaceutical or...

Ngày tải lên: 10/08/2014, 22:20