in hl 60 cell line by methacrylic monomers

... decrease in cell viability (Fig 1B) Inhibition of HL- 60 cell growth by LA is both time and dose dependent Initially, we investigated the effect of LA on cell growth inhibition Exponentially growing HL- 60 ... diminution of cell growth by 1, 2.5 and mM LA, respectively, which was accompanied by ~1, ~3% and LA induces HL- 60 cell cycle arrest by altering the expressions of specific signaling proteins ... phase cell population (28.4% in control vs 63.1% and 74% in 2.5 and mM LA treated cells) To further explore the cell cycle arrest by LA in HL- 60 cells, specific cell cycle regulatory proteins required...

Ngày tải lên: 10/08/2014, 22:20

... al.: All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein Journal of ... that ATRA suppressed the cell proliferation and induced apoptosis in GIST-T1 cells, suggesting anti-cancer effect of ATRA on GISTs The cell death inducing mechanism by ATRA in cancers has not yet ... AJ: Inhibition of c-kit receptor tyrosine kinase activity by STI 571, a selective tyrosine kinase inhibitor Blood 2000, 96:925-932 Okuda K, Weisberg E, Gilliland DG, Griffin JD: ARG tyrosine kinase...

Ngày tải lên: 10/08/2014, 10:20

... lacking an internal promoter has major deletions in the 3'LTR enhancer elements rendering its intrinsic promoter machinery transcriptionally inactive in transduced cells B Transgene expression in ... carcinoma cell line, was obtained from the American Type Culture Collection (ATCC, Manassas, VA) and was maintained in DMEM supplemented with 10%heat-inactivated FBS and 1% penicillin-streptomycin ... generated by stable transfection of the 293GPG packaging cell line as previously described [10] In brief, stable producer cells were generated by co-transfection of µg FspI-linearized control SIN vector...

Ngày tải lên: 20/06/2014, 01:20

... is mediated predominantly by CAD and partially by other nucleases Page of Methods Cell lines NPC cell lines SUNE1 and HONE1, as well as the EBV genome-positive marmoset cell line, B95-8 (gifts ... the internucleosomal linker DNA They also cleave at the borders of chromatin loops, releasing chromatin domains of sizes ≥ 50 kb [11] This chromatin loop domain structure is maintained by the interaction ... aberrations in infected cells [17] Similarly, LMP1 expression was found to induce aneuploidy in human epithelial cells [25] Knowing that EBV infection and LMP1 expression induce apoptosis in mammalian cells...

Ngày tải lên: 10/08/2014, 05:21

... expression in HL- 60 cells during ATRA-induced differentiation The microRNA profile in HL- 60 cells treated with ATRA or 0.1% alcohol was determined using a microRNA microarray as described in the ... roles in the genesis of leukemia Using a bioinformatic approach followed by in vitro experiments, we identified the microRNA gene expression profile of the AML cell line HL- 60 during ATRA induced ... treatment in HL- 60 cells, which is the first report of the involvement of miR-663 in ATRA-induced differentiation In HL- 60 cells, Pizzimenti et al reported miR-663 was upregulated by 4-Hydroxynonenal...

Ngày tải lên: 10/08/2014, 21:23

... exposure, cells were grown on inserts submersed in medium for d to grow to confluence The confluency of the cell layer was confirmed by (LSM) resulting in an average cell density of 600 0 ± 400 cells/ ... dehyde in PBS Fixed cells were treated with 0.1 M glycine in PBS for and permeabilized in 0.2% Triton X-100 in PBS for 15 at room temperature The cells were incubated with Phalloidin rhodamine (dilution ... concentrations, indicating a particle-induced lipid trafficking to the APM This finding is in accordance with studies on lipid trafficking to the APM, due to deformation stress performed in A549 cells...

Ngày tải lên: 12/08/2014, 14:20

... Gejyo F, Fujii M: PDZ domain-binding motif of human T -cell leukemia virus type Tax oncoprotein is essential for the interleukin independent growth induction of a T -cell line Retrovirology 2005, ... Tax protein in transformed cells Figure Expression of Tax protein in transformed cells Cell lysates were prepared from the indicated cells, and the amounts of Tax proteins were measured by a Western ... can not inhibit the cell growth of any HTLV-1-transformed T -cell lines [37] NFAT-inducible T -cell growth promoting factors for their growth Unlike the HTLV-2-transformed human T -cell lines sensitive...

Ngày tải lên: 13/08/2014, 09:20

... Monocytic Cell Lines 3.4 CD137L Signalling Activates Sphingosine Kinase 3.5 CD137L Signalling Does Not Induce Sphingosine Kinase Protein 3.6 Involvement Of Sphingosine Kinase In CD137L-Induced Inflammatory ... Sphingosine kinase was expressed in THP-1 cells and 65 U-937 cells Cross-linking of CD137 ligand induced sphingosine 67 kinase activity in THP-1 cells Sphingosine kinase protein level remained ... Its Ligand In Inflammation Sphingolipids 1.2.1 Sphingosine Metabolism Pathway 1.2.2 Sphingosine Kinase 1.2.3 Sphingosine Kinase Inhibitors 1.2.4 Natural Products As Sphingosine Kinase Inhibitors...

Ngày tải lên: 02/10/2015, 17:15

... intracellular domain containing proline-rich motifs Class semaphorins have PDZ-binding domain at their C-termini of intracellular domain The specific receptors for semaphorins are plexins The plexins are ... Furthermore, the cytoplasmic domain of plexin-B family has a specific sequence responsible for binding PDZ domain-containing protein (PDZ-binding domain) at the C-terminus Plexin-B1 interacts directly with ... are required from origins in neural tube to final myelination stage, and and regulated by both cell- extrinsic factors and cell- intrinsic factors Cell- intrinsic factors include olig-1, olig-2...

Ngày tải lên: 03/10/2015, 20:32

... influence of CGF on inducing apoptosis in 3T3-L1 preadipocytes was minor In contrast, CGF perturbed the cell cycle and increased cells in the G2/M cell cycle phase beginning at concentration ... SU receptor/ K+ ATP channel β -cell Increase insulin secretion Hypoglycaemia; Weight gain Intestine Decrease rate of intestinal carbohydrate digestion Gastrointestinal disturbances Fat, muscle, ... sensitivity, increasing peripheral glucose uptake, reducing plasma fatty acid concentration and enhancing adiponectin secretion [15, 54] Adiponectin influences insulin sensitivity by improving glucose...

Ngày tải lên: 05/10/2015, 19:06

... digested away in the absence of proteasome inhibitors (lane in Fig 1B, 32P vs WB) Inhibition of TPH degradation in the cell- free proteasome system by protein kinase inhibitors We examined the involvement ... phosphorylation in TPH degradation in living cells In previous works [22,23], we demonstrated in mast cell lines RBL2H3 and FMA3 that de novo biosynthesis of TPH enzyme protein was accompanied by rapid ... next h The cells were then disrupted with 1% NP-40 in 50 mM Tris/HCl (pH 7.8) containing the protease inhibitor cocktail (32P): In order to examine 32P-incorporation into proteins, the cell extracts...

Ngày tải lên: 08/03/2014, 16:20

... Rac–PAK interaction in HL- 60 (Eur J Biochem 269) 2625 Fig Increase of PAK proteins in subcellular fraction during ATRAinduced granulocytic differentiation of HL- 60 cells The differentiation of the cells ... Fig Time-dependent intracellular superoxide generation in dormant HL- 60 after starting the incubation with and without ATRA HL- 60 cells were incubated with 2¢,5¢-dichlorofluorescin for 30 and then ... without ATRA induction The increase in hydrogen peroxide, which is formed via dismutation of O2–, is also measured by using 2¢,5¢dichlorofluorescin As shown in Fig 2, HL- 60 cells gave intracellular...

Ngày tải lên: 31/03/2014, 15:20

... tubulin may be caused by an increase in PDT induced intracellular calcium (Ca2+) [52] Role of calcium in photofrin and phthalocyanine mediated photohemolysis and apoptosis in rabbit red blood cells, ... monolayer cells were incubated in growth medium, harvested and counted (using hemocytometer) after varying intervals of time Floating cells were collected separately before harvesting attached cells by ... squamous carcinoma cell lines J Photochem Photobiol B 2003, 69:107-120 Dwarkanath BS, Jain VK: Energy linked modifications of the radiation response in a human cerebral glioma cell line Int J Radiat...

Ngày tải lên: 18/06/2014, 16:20

... http://www.jhoonline.org/content/4/1/32 Page of Figure Cell growth inhibition by dasatinib and cellular signaling in a nilotinib resistant cell line (A) K562NR cells exposed to dasatinib or nilotinib for ... Cite this article as: Okabe et al.: Dasatinib preferentially induces apoptosis by inhibiting Lyn kinase in nilotinib-resistant chronic myeloid leukemia cell line Journal of Hematology & Oncology ... in the growth and apoptotic regulation of hematopoietic cells [9] Activation of SFK including Lyn may play a dominant role in the proliferation and survival of the nilotinib-resistant cell line, ...

Ngày tải lên: 10/08/2014, 21:23

... persistently HBV expressing cell line HepG2.215 and its parent cell line, a non-HBV expressing cell line HepG2 We found that cIAP1 and cIAP2 were clearly increased in the HBV expressing cells but XIAP ... as pancreatic carcinoma cells, lung cancer cells [7, 10 ], prostate cancer cells [24] and renal carcinoma cell line [28, 19, 44 ] Other interesting finding is that the cancer cells resistant to ... probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation of RNA HepG2 cell line was bought from ATCC and HepG2.215 cell line, which persistently produces...

Ngày tải lên: 02/11/2012, 11:17

... determined by dry cell weight (oven drying at 105°C for 24 h) and COD was determined by the dichromate method NBN-T 91-201 Proteins were determined by multiplying the organic nitrogen portion by ... glucosaminidase β-glucosidase α-glucosidase β-galactosidase β-glucurosidase α-galactosidase Phosphohydrolase Acid phosphatase Chemotrypsine Trypsine Cystine aminopeptidase Valine aminopeptidase Leucine ... that proteins constituted the essential of the remaining COD in the effluent of reactor R5 The higher concentration of proteins observed in the effluents of reactors could be explained by their...

Ngày tải lên: 05/09/2013, 09:08

... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins ... Thin lines represent background staining with mouse Igs (B) Histograms show background staining with no antibody (thin lines) or with rabbit Igs (thick lines) as first step in permeabilized cells ... determined by an increase in DCFH mean fluorescence intensity (Fig 2B, thick line) In addition, determin- Fig Regulation of iron related parameters by iron loading in HepG2 cells HepG2 cells were...

Ngày tải lên: 19/02/2014, 16:20

... exert their action by blocking the binding of eEF1A to its ligand in cancer cells, perhaps to zinc finger proteins involved in the modulation of cell proliferation, as proposed by Gangwani et al ... B., Tolazzi, G., Ustulin, F & Quadrifoglio, F (1998) Human cancer cell lines growth inhibition by GTn oligodeoxyribonucleotides recognizing singlestranded DNA-binding proteins Eur J Biochem 252, ... residues of lysine, arginine and histidine, could occur However it seemed probable that the eEF1A isoforms could act as a controlling event in maintaining the viability and/or promoting the growth...

Ngày tải lên: 21/02/2014, 00:20

... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... that insulin has an anti-apoptotic effect, since PKB is an important signal transducer of insulin In our laboratory, Wang et al [17] found that in a human hepatocarcinoma cell line, H7721, both insulin...

Ngày tải lên: 21/02/2014, 00:20

... This is indicated by the massive increase of cells in the S phase, the reduction in cell proliferation, and the decrease in bromodeoxyuridine (BrdU) incorporation into newly synthesized DNA In addition, ... H (2001) Growth inhibition and apoptosis induced by P2Y2 receptors in human colorectal carcinoma cells: involvement of intracellular calcium and cyclic adenosine monophosphate Int J Colorectal ... tumor cells Am J Physiol Lung Cell Mol Physiol 285, L376–L385 30 Afework M & Burnstock G (2005) Changes in P2Y2 receptor localization on adrenaline- and noradrenalinecontaining chromaffin cells in...

Ngày tải lên: 07/03/2014, 12:20