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in cell nmr of intrinsically disordered proteins in prokaryotic cells

nmr methods for the investigation of structure and transport

nmr methods for the investigation of structure and transport

Đại cương

... continuation of the research unit after the DFG funding as well as Prof Kasper, Prof Kind, Prof Nirschl, and Prof Elsner Prof Nirschl, Prof Kind, Prof Wilhelm, and Prof Elsner contributed in the ... Noise Number of coil turns Number of discretization points in ˛ direction Number of averages Number of nuclear spins Set of indexes (first index) or number of slices Set of indexes (second index) Population ... imaging to the study of the filtration process In: Stapf S, Han SI (eds) NMR imaging in chemical engineering, Wiley-VCH, Weinheim, pp 250–263 24 Stapf S, Han S (2005) NMR imaging in chemical engineering...
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Báo cáo y học:

Báo cáo y học: "Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pptx

Báo cáo khoa học

... identifying the attributes of interest, assigning levels to the attributes, presenting profiles to individuals which involve different levels of the attributes, obtaining preferences for the profiles, ... home 11 In my opinion working according the guideline requires the availability of written information that supports the oral counselling after the diagnosis 12 In my opinion working according the ... according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the guideline requires the availability of written information that...
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báo cáo khoa học:

báo cáo khoa học: " Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pot

Báo cáo khoa học

... identifying the attributes of interest, assigning levels to the attributes, presenting profiles to individuals which involve different levels of the attributes, obtaining preferences for the profiles, ... home 11 In my opinion working according the guideline requires the availability of written information that supports the oral counselling after the diagnosis 12 In my opinion working according the ... according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the guideline requires the availability of written information that...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

Báo cáo khoa học

... respectively retained in the centre of the interhelical linker and at the C-terminus of micelle-associated VpUcyt Position of VpUcyt relative to the micelle NMR signal intensities of individual VpUcyt ... K, Hoffmann S, Bauer F, Friedrich U, Kardinal C, Feller SM, Biesinger B & Sticht H (2002) Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase ... casein kinase II-mediated phosphorylation of two serines in VpU [5] VpU binding to b-TrCP does not induce its own degradation Instead, VpU degradation is reported to be b-TrCP independent and involves...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc

Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc

Báo cáo khoa học

... et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T -cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ) ... characterize ATP-binding and GTP-binding proteins in cells, we profiled purine-binding proteins on a proteome scale [40] For this purpose, we used 5¢-p-fluorosulfonylbenzoyladenosine (FSBA) (Fig ... used to profile ATP-binding and GTP-binding proteins, respectively, in the proteomes of different lymphoid cells [76] In their approach, proteins were labeled with FSBA or FSBG in cell extracts, separated...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx

Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx

Kĩ thuật Viễn thông

... Abstract Mechanical characterization of protein molecules has played a role on gaining insight into the biological functions of proteins, since some proteins perform the mechanical function Here, ... behavior of proteins such as protein unfolding mechanics Such protein unfolding experiments has been illuminated in that these studies may provide the free energy landscape of proteins related ... coarse-grained model of folded polymer chain molecules for gaining insight into unfolding mechanism with respect to folding topology, and it was shown that folding topology plays a role on the protein...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt

Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt

Báo cáo khoa học

... of a PSD95-containing complex by immobilizing a hexapeptide corresponding to the C-terminus of the NMDA receptor and subsequent binding of PSD95 along with associated proteins from mouse brain ... the regulation of synaptic plasticity by binding to the adaptor protein PSD95 This protein contains several PDZ domains one of which binds to the few most C-terminal amino acids of the NMDA receptor ... certainty of MS based protein identification now allows to access even proteins that are present in rather few copies per cell including many of the hitherto functionally unassigned proteins By...
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Báo cáo sinh học:

Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx

Hóa học - Dầu khí

... cell line SW620 expressing EpCAM (>99%) and CK (>99%) was cultured in RPMI 1640 containing mmol/L glutamine and supplemented with 20% fetal calf serum (FCS) at 37°C in air containing 5% CO2 Cells ... mM EDTA in deionized water) was used to remove erythrocytes, and the remaining cells were washed with PBS containing 0.5% bovine serum albumin (BSA) and mM EDTA Cells were resuspended in this ... double-positive cells could be detected in any of the samples We did not observe EpCAM+CK- cells (0/15), whereas we observed the presence of EpCAM-CK+ cells in samples (2/15 = 13%) when cells were...
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báo cáo hóa học:

báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx

Hóa học - Dầu khí

... cell line SW620 expressing EpCAM (>99%) and CK (>99%) was cultured in RPMI 1640 containing mmol/L glutamine and supplemented with 20% fetal calf serum (FCS) at 37°C in air containing 5% CO2 Cells ... mM EDTA in deionized water) was used to remove erythrocytes, and the remaining cells were washed with PBS containing 0.5% bovine serum albumin (BSA) and mM EDTA Cells were resuspended in this ... double-positive cells could be detected in any of the samples We did not observe EpCAM+CK- cells (0/15), whereas we observed the presence of EpCAM-CK+ cells in samples (2/15 = 13%) when cells were...
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báo cáo khoa học:

báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx

Báo cáo khoa học

... attempt of bioconjugating QD-COOH to proteins of increasingly higher pI, using cationic proteins such as cytochrome c and lysozyme However, it was observed that the pI of proteins can play a determining ... proceed readily Reductive amination is a bioconjugation technique popular in the labeling of glycoproteins Taking advantage of the polysaccharide chains within the Fc region of an antibody, it can ... an increased number of negative charges that will ultimately influence the net-charge of the conjugate The increase in peak width of the QD-BSA can be attributed to a number of factors, including...
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báo cáo khoa học:

báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx

Báo cáo khoa học

... were found 6.0-10.0 cm behind the point Page of of impact of the projectile on the gelatin block In this area, the gelatin blocks at which shots were fired with soft point bullets showed significantly ... The radiological examination of the infiltration depth of barium titanate particles within the ruptures of a temporary cavity in the gelatin block revealed a deposition of particles along the ... both types of projectiles examined In the case of the soft point projectile, there were no significant differences between the size of the temporary cavity and the infiltration depth of the barium...
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Báo cáo y học:

Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf

Báo cáo khoa học

... present data Levels of proinflammatory cytokines were also significantly higher in AS patients than in healthy individuals, in line with previous findings [18,19] Association of biomarker signatures ... osteocalcin – was revealed in the course of the biomarker analysis (Figure 4), suggesting that activation of bone-turnover pathways, exceeding that seen in RA or PsA, occurs in AS Also intriguing ... hyaluronic acid binding protein; HSP 60, heat shock protein 60; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; MMP3, matrix metalloproteinase 3; P1NP, procollagen type amino-terminal propeptide;...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

Y khoa - Dược

... progenitor cells results in increased resistance to cyclophosphamide (Wang et al., 2001) ALDH3A1 can also be expressed in certain tumor cells by inducing these cells with catechol MCF–7 cells induced ... inhibition in cancer cell lysates by ALDH3A1 inhibitors .94 Figure 28: Determination of mafosfamide ED50 values in various cell lines 97 Figure 29: Chemosensitivity experiments in cancer cells ... results in an E487K or E504K mutation Glu487, located in the bridging domain, maintains a stable scaffold and facilitates catalysis by linking together the cofactor–binding and catalytic domains...
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guide for structural maintenance of parking structures

guide for structural maintenance of parking structures

Cơ sở dữ liệu

... required Inspect deck drain function M S a Deck drains: remove debris and clean out drain; b Drain lines: check for leaks and damage; c Inspect joint sealant at top of drain AR A Install area drain ... structural inspection of parking structures A regular visual inspection of the structural and waterproofing components of the parking structure is an essential feature of a preventive maintenance ... Review by engineer if uncertain about structural effect maintained because of the structural significance of the post-tensioning function Additional information regarding corrosion of unbonded,...
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Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films

Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films

Cao đẳng - Đại học

... domain switching and the essential features of the process including finite nucleation bias in PFM capturing Molotskii et al [26, 27] proposed a closed-form solution for domain switching in the ... Department of Mechanical Engineering, National University of Singapore (NUS) under the supervision of Associate Professor Zeng Kaiyang To the best of my knowledge, all of the results presented in this ... (a) Domain geometry during tip-induced switching (b) Free energy as a function of the lateral domain size Dashed line, in a uniform electric field; solid line, in a tip-induced electric field...
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Structural characterization of nogo proteins  implications for biological functions and moleculedesign of therapeutic applications

Structural characterization of nogo proteins implications for biological functions and moleculedesign of therapeutic applications

Cao đẳng - Đại học

... Characterizations of Intrinsically Disordered Proteins Typical intrinsically disordered proteins are distinguished from ordered proteins by some unique characteristics They have specific amino sequence ... Intrinsically Disordered Protein 23 1.2.1 Reappraisal of the Protein Structure–Function Paradigm 23 1.2.2 Characterizations of Intrinsically Disordered Proteins 24 1.2.3 Biological Function of ... function information about intrinsically disordered proteins So far DisProt (Release 3.4) contains 460 intrinsically disordered proteins and 1103 disordered regions, encompassing 35 functional categories...
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Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx

Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx

Phần cứng

... method of connecting and protecting outgoing line powering pairs in  ONU (optical network unit) host cabinets • DLC remote terminal OSP pair applications Compact solution for all OSP pairs leaving ... pairs leaving DLC cabinets Saves space in the end chamber by providing cross-connect and protection in one field — up to 100% space savings in certain applications • Cabinet overlay applications ... bars Package of 10, black housing 5909 212-00 Package of 8, black housing 5909 211-00 ComProtect® protectors without ground bars Package of 25 Black housing 6659 008-30 Yellow housing 6659 008-90...
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Fundamentals of quantum mechanics for solid state electronics, optics   c  tang

Fundamentals of quantum mechanics for solid state electronics, optics c tang

Vật lý

... dynamics of charge-carriers in semiconductors, culminating finally in one of the most important building blocks of modern electronic and optical devices: the p–n junction For applications involving ... Society of America, and the Americal Physical Society, and is a member of the US National Academy of Engineering He was the winner of the Charles H Townes Award of the Optical Society of America in ... in this brief overview will be explained and justified in detail in the following chapters The purpose of the qualitative discussion in this chapter is simply to give an indication of the things...
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nanomaterials for solid state hydrogen storage, 2009, p.346

nanomaterials for solid state hydrogen storage, 2009, p.346

Vật lý

... developments in mechanical alloying for processing of nanomaterials, including those for repetitive stressing welding fracturing Fig 1.10 Mechanical alloying through repetitive cold welding and fracturing ... alloy can be ferromagnetic in spite of absolute lack of crystalline lattice (and indeed, absence of a theory of amorphous magnetism) With the invention of a new class of soft magnetic materials it ... fuel cells has been intertwined with a parallel development of alloys for electrochemical hydrogen storage in rechargeable battery cells AB5 alloys used in either hydride battery cell or in a...
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