in cell nmr in mammalian cells part 3

... imaging to the study of the filtration process In: Stapf S, Han SI (eds) NMR imaging in chemical engineering, Wiley-VCH, Weinheim, pp 250–2 63 24 Stapf S, Han S (2005) NMR imaging in chemical engineering ... It is obtained by integrating (2 .33 ) to Z t r; t/ D G t /r dt (2 .34 ) Á kr: (2 .35 ) In (2 .35 ) the definition of the wave vector Z t kD G t / dt (2 .36 ) has been introduced.10 The measuring system ... and catalyst support in a fixed bed by magnetic resonance imaging Chem Eng Sci 62(18–20, Sp Iss SI): 533 0– 533 4 DOI 10.1016/j.ces.2007. 03. 034 Grosse J, Dietrich B, Martin H, Kind M, Vicente J, Hardy...

Ngày tải lên: 29/05/2014, 18:32

... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... satisfaction Time investment 3. 88 ± 0.52 3. 86 ± 0.48 3. 81 ± 0. 53 3.81 ± 0.68 3. 75 ± 0.86 3. 73 ± 0. 83 3.52 ± 0.81 2.82 ± 0.90 4; 2–5 4; 2–5 4; 2–5 4; 1–5 4; 1–5 4; 1–5 4; 1–5 3; 1–5 SD: standard ... Status of the guideline Time investment* RI 12 8500 (1) 10 2877 (2) 5 832 (3) 0656 (4) 4579 (5) 8002 (6) 57 43 (7) 1716 (8) 8 736 (9) 18 13 (10) 0 234 (11) 8478 (12) 4884 ( 13) 38 83 (14) 134 8 (15) 2989 (16)...

Ngày tải lên: 11/08/2014, 05:21

... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... satisfaction Time investment 3. 88 ± 0.52 3. 86 ± 0.48 3. 81 ± 0. 53 3.81 ± 0.68 3. 75 ± 0.86 3. 73 ± 0. 83 3.52 ± 0.81 2.82 ± 0.90 4; 2–5 4; 2–5 4; 2–5 4; 1–5 4; 1–5 4; 1–5 4; 1–5 3; 1–5 SD: standard ... Status of the guideline Time investment* RI 12 8500 (1) 10 2877 (2) 5 832 (3) 0656 (4) 4579 (5) 8002 (6) 57 43 (7) 1716 (8) 8 736 (9) 18 13 (10) 0 234 (11) 8478 (12) 4884 ( 13) 38 83 (14) 134 8 (15) 2989 (16)...

Ngày tải lên: 11/08/2014, 16:21

... Vpr( 13 33 ) peptide in micelles Eur J Biochem 268, 38 9 39 5 54 Engler A, Stangler T & Willbold D (2002) Structure of human immunodeficiency virus type Vpr (34 –51) peptide in micelle containing aqueous ... struc- NMR structure of micelle-associated HIV-1 VpUcyt 23 24 25 26 27 28 29 30 31 32 33 ture of the hydrophilic region of HIV-1 encoded virus protein U (Vpu) by CD and 1H NMR spectroscopy Int J ... 4. 430 4. 430 1.509 4.070 7 .33 2 7 .33 2 8.641 8.641 8.641 2. 139 ; 1.774 2.109; 2.457; 1.846; 4.777 4.207 1.017 1.017 1.725 0. 933 2.064 2. 232 2.461 1.940 Calculation of the VpUcyt structure in micelle...

Ngày tải lên: 18/02/2014, 06:20

... in human Jurkat T -cells grown in stable isotope labeling by amino acids in cell culture (SILAC) medium supplemented with either natural, light 12C6-arginine or heavy 13C6-arginine [50] Arginine ... 18O-labeling [52] was used to characterize in vivo protein processing in Fas-induced apoptotic Jurkat cells [34 ] In this study, 93 cleavage sites in 71 different proteins were characterized in a ... characterize ATP-binding and GTP-binding proteins in cells, we profiled purine-binding proteins on a proteome scale [40] For this purpose, we used 5¢-p-fluorosulfonylbenzoyladenosine (FSBA) (Fig 3B), a known...

Ngày tải lên: 18/02/2014, 16:20

... mechanism. 23 Eom et al24,25 provided the coarse-grained model of folded polymer chain molecules for gaining insight into unfolding mechanism with respect to folding topology, and it was shown that folding ... discarded.24 ,33 Once a constant, discrete strain tensor Δε0 is prescribed to a unit cell containing protein crystal, the displacement vector u due to strain Δε0 for a given position vector r of a protein ... the initial conformation of a protein crystal as the native conformation deposited in protein data bank (PDB) for a given protein crystal in a unit cell Such initial confirmation for a protein...

Ngày tải lên: 22/02/2014, 08:20

... cadherin-catenin complex in vitro with recombinant proteins J Cell Sci 107, 36 55 36 63 Rimm, D.L., Koslov, E.R., Kebriaei, P., Cianci, C.D & Morrow, J.S (1995) Alpha (E) -catenin is an actin-binding and ... Elucidation of protein–protein interactions using chemical cross- linking or label transfer techniques Curr Opin Chem Biol 4, 28 33 17 Medina, D., Moskowitz, N., Khan, S., Christopher, S & Germino, J (2000) ... known to be involved in signaling processes related to the regulation of synaptic plasticity by binding to the adaptor protein PSD95 This protein contains several PDZ domains one of which binds to...

Ngày tải lên: 31/03/2014, 07:20

... cultured in RPMI 1640 containing mmol/L glutamine and supplemented with 20% fetal calf serum (FCS) at 37 °C in air containing 5% CO2 Cells were harvested by incubation with phosphate-buffered saline ... EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38 % (14 /37 ) of peripheral blood samples, and the median of the number of these cells was (range: 1- 43) /10 mL blood EpCAM+CKCD45- cells were ... permeabilize the cells, pellet was resuspended in mL of a sterile solution containing 0.1% saponin, 0.05% NaN3 in Hanks’ Balanced Salt Solution (SAP buffer) Cells were centrifuged at 200 × g for minutes;...

Ngày tải lên: 18/06/2014, 19:20

... cultured in RPMI 1640 containing mmol/L glutamine and supplemented with 20% fetal calf serum (FCS) at 37 °C in air containing 5% CO2 Cells were harvested by incubation with phosphate-buffered saline ... EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38 % (14 /37 ) of peripheral blood samples, and the median of the number of these cells was (range: 1- 43) /10 mL blood EpCAM+CKCD45- cells were ... permeabilize the cells, pellet was resuspended in mL of a sterile solution containing 0.1% saponin, 0.05% NaN3 in Hanks’ Balanced Salt Solution (SAP buffer) Cells were centrifuged at 200 × g for minutes;...

Ngày tải lên: 20/06/2014, 03:20

... increasing use in the separation and characterization of inorganic nanoparticles (Ag, Au, TiO2, Al2O3, Fe2O3) [ 13- 17], polystyrene microspheres [18], biomolecules (proteins, peptides) [1 930 ], QDs [31 ], ... bioconjugating QD-COOH to proteins of increasingly higher pI, using cationic proteins such as cytochrome c and lysozyme However, it was observed that the pI of proteins can play a determining factor in ... conjugates' affinity to each other via strong biotin-streptavidin interactions However, present publications reporting the use of QDs in CE-based immunoassays are very preliminary, due in part to a...

Ngày tải lên: 11/08/2014, 00:22

... titanate particles is a simple and reproducible method of systematic examination in the field of terminal ballistics The model permits the infiltration depth of exogenous particles leading to contamination ... for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation Head & Face Medicine 2011 7:18 Competing interests The authors declare ... smaller in diameter This can be explained by cavitation effects in connection with particle inertia This might explain the fact that the suction effect of the negative pressure wave within the...

Ngày tải lên: 11/08/2014, 20:21

... 102 126 701 117 36 5 1 83 134 99 37 5 154 133 33 1 412 659 92 8 03 329 32 4 39 4 894 829 158 37 7 170 37 8 37 9 32 7 477 152 1 13 138 409 449 37 1 36 6 33 4 812 730 742 105 472 172 191 174 1 73 367 782 750 175 ... 665 38 4 38 2 32 3 39 1 35 2 155 37 0 37 3 16 446 1 23 145 167 460 119 829 152 894 750 102 37 5 1 73 1 83 175 412 812 782 165 170 36 5 191 174 126 8 03 379 105 99 1 13 158 138 449 409 37 1 172 133 92 36 6 33 4 ... 154 39 4 33 1 92 701 102 8 03 1 83 117 32 9 36 5 32 4 751 449 37 8 32 7 477 37 1 138 33 4 409 105 172 Figure Chandra et al 99 1 13 158 152 37 9 742 126 1 73 174 37 5 165 134 782 36 7 412 170 829 175 812 133 36 6...

Ngày tải lên: 12/08/2014, 17:22

... (ENAMINE– T5 633 3 437 ), B2– (ENAMINE– T5477154), B4– (ENAMINE–T 539 5179), B5– (ENAMINE–T5 237 7 43) , B6– (ENAMINE–T051261 53) , B9– (ENAMINE– T5804455), B10– (ENAMINE– T56625 23) , B11– (ENAMINE– T56556 73) , ... dimensional map showing CB25 binding with ALDH3A1 84 Figure 25: ALDH expression in A549, SF767, HEK2 93 and CCD13Lu cells 88 Figure 26: Quantitation of ALDH1A1 and ALDH3A1 in A549 and SF767 cells 91 ... B 13 (ENAMINE– T6245968), B15– (ENAMINE– T6241917), B16– (ENAMINE– T60 537 24), B17– (ENAMINE– T6 036 772), B18– (ENAMINE– T6 032 0 83) , B19– (ENAMINE– T6266966), B21– (ENAMINE– T6560452), B22– (ENAMINE–...

Ngày tải lên: 24/08/2014, 09:51

... information regarding concrete durability Testing may include compressive strength, chain dragging, and half -cell testing to locate GUIDE FOR STRUCTURAL MAINTENANCE OF PARKING STRUCTURES 36 2.2R -3 active ... required Inspect deck drain function M S a Deck drains: remove debris and clean out drain; b Drain lines: check for leaks and damage; c Inspect joint sealant at top of drain AR A Install area drain ... 36 2.2R-2 ACI COMMITTEE REPORT 3. 3 .3 Stair and elevator towers 3. 3.4—Exposed metals Chapter 4—General maintenance considerations, p 36 2.2R-9 4.1—Housekeeping and cleaning requirements...

Ngày tải lên: 24/10/2014, 21:59

Ngày tải lên: 14/11/2014, 06:20

... v 2 .3. 1 Thermodynamics of Domain Switching 28 2 .3. 2 Domain relaxation 32 References Chapter 3: Literature Review-Kelvin Probe Force Microscopy 35 38 3. 1 Kelvin Probe Force Microscopy 39 3. 2 Detection ... cross indicating poling point, (b) as-grown domain structure, (c) domains immediately after poling, and (d–f) evolution of domain structure after 4, 90, and 140 after poling Figure 3. 1 Basic principle ... ε11ε 33 ) and γ = ε 33 / ε 11 Here, domain size and energy are found to be re = 0 .34 2d 2 /3 (bc) −1 /3, le = 0.2d/b, and Em = −0.205d 5 /3 (bc) −1 /3 [26, 27] The activation energy for domain nucleation...

Ngày tải lên: 10/09/2015, 08:41

... Phenomenon 30 1 .3. 3 Chemical Shift 31 1 .3. 4 J Coupling 31 1 .3. 5 Nuclear Overhauser Effect (NOE) 34 1 .3. 6 NMR Relaxation Parameters 35 1 .3. 7 General Strategies of NMR Structure Determination 36 1 .3. 7.1 ... Protein sample preparation 36 1 .3. 7.2 NMR spectroscopy 38 1 .3. 7 .3 Resonance assignment 38 1 .3. 7.4 Collection of conformational constraints 40 1 .3. 7.5 Calculation of the 3D structure 40 1 .3. 7.6 ... Water 122 3. 5.1 Nogo-66 fragments 1 23 3.5.2 Extracellular receptor (NgR) domains 126 3. 5 .3 Other proteins 127 3. 5.4 Discussion 129 3. 6 Structural Characterization of NgBR 134 3. 6.1 Cloning and...

Ngày tải lên: 14/09/2015, 12:53

... connecting and protecting outgoing line powering pairs in  ONU (optical network unit) host cabinets • DLC remote terminal OSP pair applications Compact solution for all OSP pairs leaving DLC cabinets ... cabinets Saves space in the end chamber by providing cross-connect and protection in one field — up to 100% space savings in certain applications • Cabinet overlay applications Compact 30 7 type replacement ... 30 sec operation ( 36 .5 mm) (2.64mm) ( 9.4 mm) ( 20.6 13 mm) ( 8.7 mm) ( 11.4 mm) (17.8mm) ComProtect dimensions ComProtect unit inserted into Series Block (21 .33 mm) (61.9mm) (2.64mm) • ons Single-Pair...

Ngày tải lên: 10/12/2013, 18:15

... Broglie waves 3. 1 Free particles and de Broglie waves 3. 2 Momentum representation and wave packets 3. 3 Problems 33 33 37 39 Particles at boundaries, potential steps, barriers, and in quantum wells ... definition the corresponding integral and it is an ordinary number As far as the final numerical result of the integral is concerned, the information on what coordinate system is used in carrying ... value In the language of quantum mechanics, pffiffiffiffiffiffiffiffiffi Áx  Áx2 as defined in (2 .3) is called the ‘‘uncertainty’’ in the position x of the particle when it is in the state C(x) The definitions...

Ngày tải lên: 17/03/2014, 14:41